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3.4.2 Bypassing the Solid
Load Cartridge
(injection valve
equipped systems
only)
3.4.3 About Solvent Level
Detection (systems
equipped with this
option only)
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
Isocratic Hold – The I
the current value while the system continues to operate. Note
that holding the run extends the separation length. While in the
isocratic hold state,
the button is renamed
and
you can resume the gradient by clicking the button, or stop
the separation by clicking the S
the separation, the system continues with the gradient curve at
the %B when the system was placed in isocratic hold.
Stop Flow, Terminate, and Reset – The S
suspends the entire separation. Unlike the I
state, the pump, peak detection, and fraction collection will stop.
While in the S
F
TOP
LOW
• resume the run by clicking the S
• skip the rest of the separation by clicking the
T
button which proceeds with the air purge (if
ERMINATE
the system is equipped with this feature), or
• end the run by clicking the R
the data from the screen and displays the now-truncated
temporary method.
The advantage of T
ERMINATE
advances to the air purge line flush and detector wash, while
R
performs none of these important maintenance functions.
ESET
If R
is selected, it may be useful to manually flush the lines
ESET
so that strong solvent remaining in the lines doesn't affect subse-
quent separations.
Tube Advance – This button advances the fraction collector to
the next tube position after the delay volume from the detector to
the fraction collector has elapsed. This ensures the fractionation
matches the detector signal at the time the button was pressed.
Occasionally, impurities precipitate in the solid load cartridge as
compounds are purified. This may cause high back pressures
resulting in reduced flow rates and long run times. Clicking this
button changes the injection valve position, which removes the
cartridge from the solvent path and relieves the back pressure.
Any compound that hasn't eluted from the solid load cartridge
will remain in the cartridge and not be purified.
When solvent level sensing is enabled on the Instrument Config-
uration tab of the T
OOLS
will estimate the solvent volume usage by measuring the solvent
level in the container and the monitoring the usage rate. The
system continues to refine this estimate of available solvent
during operation.
At the beginning of a run, the system calculates the amount of
solvent required to complete the run. The system will alert you if
it detects that there is not enough solvent at the start of the run.
Likewise, the system will alert you during the run if the esti-
mated usage rate or the measured level indicates that there is
CombiFlash NextGen Systems
Section 3 Operation
H
button holds the %B at
SOCRATIC
OLD
C
ONTINUE
F
button. If you resume
TOP
LOW
TOP
condition, you can:
button, or
TART
button, which clears
ESET
over R
is that T
ESET
> C
window, the system
ONFIGURATION
G
RADIENT
F
button
LOW
H
SOCRATIC
OLD
ERMINATE
3-7
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