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CombiFlash NextGen Systems
Section 5 Maintenance
Figure 5-2 Top shelf drain hole
5.5 Flow Cell Cleaning
5.5.1 Post Separation
Figure 5-3 Default post-separation column and flow cell
flush
5-4
As a preventive measure, all default column methods finish the
separation run with a high percentage of solvent B (Figure 5-3).
This brief time (one to six column volumes) of strong solvent
flushes residual compounds from the column, flow cell, and
internal tubing.
Skipping the post-separation flush may cause residual com-
pounds to build up and crystallize, which might result in:
• Cross contaminating later separation runs.
• Higher operating pressures.
• Reduced flow cell lamp energy.
• A noisy baseline on the absorbance trace.
• Frequent messages recommending flow cell cleaning
(Figure 5-4).
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
Vacuum or pressurized air applied
to the outlet end of the drain
tube must exist at the top self
drain hole.
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