Teledyne ACCQPrep HP125 Installation And Operation Manual
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Teledyne ACCQPrep HP125 Installation And Operation Manual

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ACCQPrep HP125
Installation and Operation Guide
60-5233-812
Copyright © 2017 Teledyne Technologies Incorporated. All rights reserved.
Rev. C, January 2019

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Summary of Contents for Teledyne ACCQPrep HP125

  • Page 1 ACCQPrep HP125 Installation and Operation Guide 60-5233-812 Copyright © 2017 Teledyne Technologies Incorporated. All rights reserved. Rev. C, January 2019...
  • Page 3 OSHA Guide 1910.1000. To reduce those levels to a safe exposure, Teledyne Isco recommends that you place the system in a laboratory hood designed for the purpose of ventilation. This hood should be constructed and operated in accordance with federal state and local regulations.
  • Page 4 ACCQPrep HP125 Installation and Operation Guide Safety Warnings DANGER DANGER – limited to the most extreme situations to identify an imminent hazard, which if not avoided, will result in death or serious injury. Hazard Symbols The equipment and this manual use symbols used to warn of hazards.
  • Page 5 ACCQPrep HP125 Installation and Operation Guide Safety Warnings For Additional Technical assistance for the Teledyne Isco ACCQPrep HP125 can Information be obtained from: Teledyne Isco 4700 Superior St. Lincoln NE 68504 Phone: (800) 775-2965 or (402) 464-0231 Fax: (402) 465-3001...
  • Page 6 ACCQPrep HP125 Installation and Operation Guide Safety Warnings...

  • Page 7: Table Of Contents

    ACCQPrep HP125 Table of Contents Section 1 Introduction 1.1 Overview ............1-1 1.2 Product Overview .
  • Page 8 6.5 ACCQPrep HP125 Maintenance ........
  • Page 9 ACCQPrep HP125 Table of Contents 6.8.1 ESI and APCI Removal from PurIon S and PurIon L ....6-22 6.8.2 ESI and APCI Replacement PurIon S and PurIon L ....6-22 6.8.3 Cleaning the Ionization Source Capillary .
  • Page 10 ACCQPrep HP125 Table of Contents...

  • Page 11: Section 1 Introduction

    The ACCQPrep HP125 is available in different configurations to meet your needs. An optional Solvent Selector Valve Module sup- ports up to three choices for solvent A and three different choices for solvent B.
  • Page 12 ACCQPrep HP125 with optional ELSD – T h i s s y st em h a s the same high performance features as the ACCQPrep HP125 system, but includes an internal evaporative light scattering detector (ELSD).

  • Page 13: Operating Overview

    The system also supports TCP/IP communication. This allows direct control of the system by an external computer between Ethernet ports of the ACCQPrep HP125 system and the com- puter. TCP/IP communication also allows remote control of the system via an established network.

  • Page 14: Specification

    ACCQPrep HP125 Section 1 Introduction 1.5 Specification Table 1-1 ACCQPrep HP125 Dimensions of ACCQPrep 27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm) (Footprint without column or column cover installed) Dimensions of ACCQPrep AS 2x1 18.9 x 14.0 x 19.3 in (48.0 x 35.6 x 49.0 cm) Weight 93.2 lbs (42.3 kg)
  • Page 15 Maximum Altitude 2000 meters Note 1. All specifications are subject to change. Table 1-2 ACCQPrep HP125 with PurIon System Dimensions (H x W x D) ACCQPrep: 27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm) Mass Spectrometer 26 x 11 x 22 in (66 x 28 x 56 cm)
  • Page 16 ACCQPrep HP125 Section 1 Introduction...

  • Page 17: Section 2 Installation

    ACCQPrep HP125 Section 2 Installation 2.1 ACCQPrep Installation The following section will cover the installation, connection of the pump, and a review of the priming steps. Note When the system ships, it will ship in two boxes to mini- mize the weight. One box will contain the unit and the other box will have the pump and assorted accessories.

  • Page 18: Pump Installation

    ACCQPrep HP125 Section 2 Installation 2. Push the arm to the left or right to remove the spacer. 3. Store the bracket and spacer. These parts should be rein- stalled if the system must be shipped again. When you are ready to place the system, be sure it is situated in such a way that the power cord is accessible to disconnect in case of malfunction.
  • Page 19 ACCQPrep HP125 Section 2 Installation Figure 2-3 Pump compartment with electrical connection shown 4. When the pump assembly is most of the way in the pump compartment, connect the electrical connections on each side of the pump. First, connect the black connection on the left side.

  • Page 20: Solvent Line Installation

    ACCQPrep HP125 Section 2 Installation 5. Once the electrical connections are complete, push the pump assembly the rest of the way into the pump compart- ment until the holes in the front of the pump assembly are aligned with the corresponding holes in the case bottom.

  • Page 21: Fluid Line Installation

    ACCQPrep HP125 Section 2 Installation 3. Connect the shorter FEP inlet solvent line to the left side pump’s PEEK tee. Slide the open side of the wrench (included) over the tubing and FEP fitting and make small turns as to not bend the tubing (Figure 2-8).

  • Page 22: Accqprep Equipped With Optional Elsd

    Section 2 Installation 2.2.4 ACCQPrep Equipped with Optional ELSD Note Teledyne ISCO recommends >99% pure nitrogen from a source that can deliver 2.5 SLPM at 60 to 70 psi. Figure 2-9 Location of ELSD gas port (Nitrogen input line circled) The ACCQPrep ELSD system has several additional connections.
  • Page 23 ACCQPrep HP125 Section 2 Installation 2. ELSD operation requires a carrier gas. To connect the car- rier gas to the ACCQPrep: • Locate the inch O.D. FEP tubing (PN:023-0503-02) from the accessory package. • Push one end of the tubing into the NITROGEN INLET (Figure 2-9) port on the back of the system.

  • Page 24: Priming Steps

    CAUTION Discontinue use of the ACCQPrep if liquid is present at the Pump Drain. Contact Teledyne ISCO technical service for assistance with correcting the leak. 2.2.5 Priming Steps The system will require priming with the desired solvents. To prime the system: 1.

  • Page 25: Installing The Manual Injection Port

    ACCQPrep HP125 Section 2 Installation Figure 2-13 Turn the valve to the Prime position • If the solvent select option is not installed, the system will immediately prime both solvent lines. • If the solvent select option is installed, a dialog box opens when the valve is switched to the P position.

  • Page 26: Installing The Autosampler

    ACCQPrep HP125 Section 2 Installation Connect the included check valve(209016629) to the inject valve waste line. Ensure that the arrow on the check valve points toward the waste container. This check valve ensures sample doesn’t run from the port when the injection syringe is removed.
  • Page 27 ACCQPrep HP125 Section 2 Installation Figure 2-16 Z-Drive mounted on the Y-axis carriage 3. Remove the two screws from the Y axis carriage. Slide the Z-Drive onto the arm until the two holes align with the matching holes in the Y-axis carriage.
  • Page 28 ACCQPrep HP125 Section 2 Installation Figure 2-18 Z-Drive rotation 6. Mount the wash well by placing it into the receptacle located on the right-rear portion of the AutoSampler Mod- ule bed (Figure 2-19). Overflow drain Wash station Waste line Wash station...
  • Page 29 ACCQPrep HP125 Section 2 Installation Note The wash fluid peristaltic pump uses Pharm-A-Line tubing. Please ensure the wash fluid is compatible with this tubing, such as methanol. Figure 2-20 AutoSampler Module peristaltic pump with tubing correctly attached 9. Connect the tube labeled “Wash Waste” to the tube labeled “4”...
  • Page 30 ACCQPrep HP125 Section 2 Installation Auto Injector Optional Auto Auto External Sampler Sampler Detector Power Power Ethernet Column Valve To bottom Optional column port Waste Auto Auto Check Valve Auto Sampler Probe Sampler Sampler Wash Station Wash Pump 1 – Wash Inlet 2 –...
  • Page 31 ACCQPrep HP125 Section 2 Installation Auto External Sampler Detector Power Auto Sampler Waste Level Waste Sense Solvent Inlet Main Power Solvent Level Sensing Drain Circuit Breaker Figure 2-23 Diagram of the AutoSampler and ACCQPrep USB connections (See Appendix A, Figure A-2 for a larger diagram) 13.

  • Page 32: Establishing The Fluid Connections

    ACCQPrep HP125 Section 2 Installation Note The power supply accompanying the AutoSampler will not be used, the AutoSampler will receive its power supply through the ACCQPrep. 15. Power for the AutoSampler is provided by the ACCQPrep. On the rear panel of the ACCQPrep, the power cord is coiled and retained (Figure 2-24).
  • Page 33 ACCQPrep HP125 Section 2 Installation Probe tubing Figure 2-26 Probe tubing affixed to the side of the ACCQPrep 4. Install the included 18 mm test tube in the receptacle at the right rear corner of the AutoSampler platform just to the left of the wash well (Figure 2-27).
  • Page 34 ACCQPrep HP125 Section 2 Installation 2. Turn O the power to the ACCQPrep. A red light on the AutoSampler will light up, the arm will move to the H position, and then the light will turn blue (Figure 2-29). Figure 2-29 Power switch for the ACCQPrep Prime AutoSampler Wash 1.

  • Page 35: Column Installation

    ACCQPrep HP125 Section 2 Installation Overflow drain Wash station Waste line Wash station supply line Figure 2-31 Wash station and overflow drain 2.5 Column Installation The ACCQPrep is compatible with HPLC columns ranging from 4.6 mm up to 50 mm diameter using typical 10-32 HPLC style fit- tings.
  • Page 36 ACCQPrep HP125 Section 2 Installation Figure 2-32 Column Mounting Parts To install a column onto the ACCQPrep: 1. Select the proper sized column mount and simply slip the mount into one of the two column mounting tracks on the side of the ACCQPrep (Figure 2-33).

  • Page 37: Column Configuration

    ACCQPrep HP125 Section 2 Installation Figure 2-34 Column with inlet and outlet lines connected Note Metal tubing and fittings for the column connection are recom- mended. The high pressure capability of the ACCQPrep could cause a rupture if polymer tubing or fittings are used and a blockage occurs.

  • Page 38: Installation Troubleshooting

    ACCQPrep HP125 Section 2 Installation Note Mixed reverse phase and normal phase solvents may require manual flushing of the pumping system between separations. • If the CSVM was ordered with the instrument, it will arrive installed in the ACCQPrep. • If you decide to add this capability at a later time, this...
  • Page 39 ACCQPrep HP125 Section 2 Installation AutoSampler control not visible on the ACCQPrep software – The ACCQPrep only loads the AutoSampler control features if it can detect the presence of the AutoSampler Check the AutoSampler pilot light (upper right corner of AutoSampler) to determine the next step.
  • Page 40 ACCQPrep HP125 Section 2 Installation Table 2-1 Installation Qualification Checklist Installer Operator Step Section Description Initials Initials Unpacking the unit Instrument location Connect power Install AutoSampler (if applicable) Set up solvent lines Set up waste lines ELSD/PurIon connections and gas supply (if...

  • Page 41: Section 3 Configuration

    ACCQPrep HP125 Section 3 Configuration 3.1 Configuration of the ACCQPrep Instrument Configuration Instrument Configuration can be found under: > C > I OOLS ONFIGURATION NSTRUMENT ONFIGURATION Figure 3-1 Instrument configuration screen From this menu you can select the solvents for both solvent A and B (Figure 3-1).

  • Page 42: Network Configuration

    ACCQPrep HP125 Section 3 Configuration Naming the System – Under the ACCQP option, you can name the system so when reports print you know what system was used. Setting Time Zones and Date/Time – The time zone will auto- matically change the time during daylight savings time. Set the Date/Time to your local day and current time since all separa- tions are tagged with the date and time of operation.

  • Page 43: Prep Hplc

    ACCQPrep HP125 Section 3 Configuration reviewing a previous separation, this information isn’t available on the screen, but many times, the profile of the trace is adequate for troubleshooting. Show Absorbance During Equilibration – Shows absorbance of the system during equilibration to verify baseline stability.

  • Page 44: Define A Column

    ACCQPrep HP125 Section 3 Configuration 3.4.1 Define a Column To define a column: 1. Press the N button. This will open a keyboard where you can enter the name of the column. 2. Once the name is complete, press E NTER 3.

  • Page 45: Creating Other Methods

    ACCQPrep HP125 Section 3 Configuration Figure 3-2 Prep HPLC screen 3.4.3 Creating Other Methods created in this screen are associated with a particular Methods column when created. If multiple methods are associated with a single column, these methods can b e easily accessed whenever a particular column is selected.

  • Page 46: Creating Focused Gradients

    ACCQPrep HP125 Section 3 Configuration Figure 3-3 Define Methods screen 3. Press N 4. Enter in the name you have chosen. 5. Once named, press E NTER 6. The newly created method uses the default method parameters. To modify the new method: a.

  • Page 47: Section 4 Operation

    ACCQPrep HP125 Section 4 Operation 4.1 Introduction The ACCQPrep HP125 is unique in the Preparative HPLC market. There are several methods of operation possible that can be selected to fit your needs. Methods contain all of the information used during a separation of a single sample, such as gradient profile, detection and peak cutting parameters, and fraction size.

  • Page 48: Starting A Separation

    ACCQPrep HP125 Section 4 Operation 4.3 Starting a Separation To separate a single sample, press P to start a separation. The M screen is displayed. This INIMUM EQUIREMENTS allows you to select an injection technique and select where the fractions will be collected (the starting rack and tube position). In addition, the system projects the amount of solvent required and the separation duration.
  • Page 49 ACCQPrep HP125 Section 4 Operation 3. If you want to automatically perform multiple injections (or perform a single injection using the AutoInjector Mod- ule), select the A drop down as shown in Fig- NJECTOR ure 4-1. This is the default selection if an AutoInjector Module is present.
  • Page 50 ACCQPrep HP125 Section 4 Operation Figure 4-2 Selecting the sample volume and number of injections PeakTrak will divide the total volume by the number of injec- tions to determine the injection volume for each separation. If this volume exceeds 50% of the loop volume, a warning message will appear recommending a change in the parameters.

  • Page 51: Autoinjection Operating Steps

    ACCQPrep HP125 Section 4 Operation 6. At the completion of the sequence, the final separation results will be displayed. To easily view previous separa- tions, press the R key to return to the H screen. EWIND 7. Select F to view previous separations.
  • Page 52 ACCQPrep HP125 Section 4 Operation over fluid from a previous separation and fills the loop with the initial gradient conditions from the current separation. This prevents any strong solvent remaining in the loop from affecting the current separation. 3. Near the end of the equilibration, the inject valve moves to the sample load position.

  • Page 53: Separation Using An Autosampler Module

    ACCQPrep HP125 Section 4 Operation 4. 10 mL of weak solvent is aspirated through the probe to wash away any remaining strong solvent from the probe so it cannot interfere with future separations. 4.3.3 Separation using an Verify that the wash station contains fluid. If not, go to...
  • Page 54 ACCQPrep HP125 Section 4 Operation Sample Name – Allows you to name each sample. Naming is optional. If left blank, the sample is named based on the date and time the separation was started. Column (drop down menu) – I f a C o l u m n S e l e c t o r Va l v e Module (CSV-4) is installed, this menu allows you to select a sep- aration column and associated method for use.

  • Page 55: Autosampler Injection Techniques

    ACCQPrep HP125 Section 4 Operation ever, by touching the sample name corresponding to that run. 5. During the separation, completed fraction racks can be removed when full and replaced with empty racks to allow continuous separations. If a single rack containing frac-...
  • Page 56 ACCQPrep HP125 Section 4 Operation injection minimizes the risk of sample crash by placing a small amount of a user selected solvent on each end of the sample fluid. All sample injection steps are listed below for reference. Default Injection Sequence 1.
  • Page 57 ACCQPrep HP125 Section 4 Operation 1. The inject valve will be moved to bypass to prevent con- tamination of the loop during the cleaning process. 2. Air is drawn into the probe to eliminate any remaining sol- vent in the probe.

  • Page 58: Fraction Collection With The Autosampler

    ACCQPrep HP125 Section 4 Operation volume of the sample probe. Since the loop is still in the separation position, the wash solvent is sent to waste. 10. The loop is switched to the load position. 11. The programmed injection volume is aspirated into the sample loop.

  • Page 59: Operation Troubleshooting

    ACCQPrep HP125 Section 4 Operation When the system moves from the ACCQPrep fraction racks to the AutoSampler fraction racks, the fluid uses an alternative path. This leaves a portion of tubing with fluid remaining for the fraction collector previously used. To prevent loss of any com-...
  • Page 60 ACCQPrep HP125 Section 4 Operation · Inadequate priming of the pump may cause the A or B solvent to flow at lower rates. This may be due to sticking check valves. Open the front panel and tap the check valves with a wrench while priming to assist in bubble passage through the pump.
  • Page 61 ACCQPrep HP125 Section 4 Operation should be visible with a small gap between the rods. · This is not a lamp or detector hardware problem. These failures will have a corre- sponding error message. Peaks elute too early – • Solvent A not being delivered properly. Check solvent...
  • Page 62 ACCQPrep HP125 Section 4 Operation • Impurities in solvent; evaluate with runs with no sample injection to determine if impurity is from the sample. Make a run with a standard injection volume, then double the equilibration time. If the peak in question become larger, it is solvent related.
  • Page 63 ACCQPrep HP125 Section 4 Operation · Semivolatile compounds may be difficult to detect or may benefit from modification of the ELSD sensi- tivity, gain or operating temperatures. Refer to the discussion on ELSD operation in section 5.2.7 before adjusting the ELSD operating temperatures.
  • Page 64 ACCQPrep HP125 Section 4 Operation a. Remove guard column (if installed) and check back pressure. If this corrects the problem, replace the guard column or following the wash- ing procedure below. b. Remove preparative column and check back pressure. c. If the column is clogged, try flushing with stron- ger solvent than mobile phase: (this procedure may damage polymeric reverse phases).

  • Page 65: Section 5 Peaktrak

    ACCQPrep HP125 Section 5 PeakTrak 5.1 Overview PeakTrak is the software that controls the ACCQPrep. This section discusses the basic functionality of PeakTrak when operated via the touchscreen or a remote browser window. 5.1.1 PeakTrak Window The M window displays the current M .

  • Page 66: Run File

    Default Methods When PeakTrak is started, the system loads a D EFAULT ETHOD contains Teledyne Isco’s recommended basic EFAULT ETHOD and advanced settings for the use of a default column (as selected in the config screen). There can be a D...
  • Page 67 ACCQPrep HP125 Section 5 PeakTrak 5.2.1 File The File menu lists the following commands: New – Select the N command to open a new method file. PeakTrak will open the M window using the default program settings for the default configured column size.

  • Page 68: File

    ACCQPrep HP125 Section 5 PeakTrak download window so you can select a location and enter a file name. (You can open and print PDF files with Adobe Reader.) Save Run As TXT – Choose this command to save the displayed run on a remote computer’s hard drive or a USB Flash storage device connected to the system.
  • Page 69 ACCQPrep HP125 Section 5 PeakTrak 5.2.3 MS The MS menu item is only available when a PurIon Mass Spectrometer is added to the ACCQPrep system. The following options are available: Method Development – Choose this command to open the MS M...

  • Page 70: Tools

    ACCQPrep HP125 Section 5 PeakTrak WARNING Do not shut down or disconnect the vacuum line to the roughing pump until the PurIon is fully vented as indicated by PeakTrak. 5.2.4 Tools The Tools menu has the following options: Manual Control – Choose this command to open the window.

  • Page 71: Help

    ACCQPrep system software. Export Log Files – During operation, the system writes operating data to a log file. Teledyne Isco service personnel can interpret this data to optimize system performance or to troubleshoot difficulties. Teledyne Isco recommends that you use the menu command only when advised by a qualified service technician.

  • Page 72: Peaktrak Windows

    ACCQPrep HP125 Section 5 PeakTrak 5.2.6 PeakTrak Windows This section contains descriptions of the windows used for most PeakTrak tasks. In this section you will find information on the following: • M INDOW • M ETHOD DITOR • G RADIENT PTIMIZER •...
  • Page 73 ACCQPrep HP125 Section 5 PeakTrak rather than a pan function.) The gradient plot area also provides a convenient method to edit the gradient curve. You can click and drag any of the points to change the shape of the curve and use the buttons at the bottom of the plot area to insert and delete points.
  • Page 74 ACCQPrep HP125 Section 5 PeakTrak Spaces are not allowed in the name. If you do not type a Sample Name, PeakTrak will generate a date/time stamp for the name when you click the P button to begin the run. • Flow Rate — Type or select the desired flow rate for the run.
  • Page 75 ACCQPrep HP125 Section 5 PeakTrak Unlike the R button, the F button EWIND ORWARD will not modify the current method’s run length setting. • F — Click this button to jump to the next ORWARD step of a run. Once you’ve started a run, the system performs several steps.

  • Page 76: Method Editor

    ACCQPrep HP125 Section 5 PeakTrak You can return to the rack and tube display by clicking the D button. ISPLAY 5.2.7 Method Editor The M window has several sections. The B ETHOD DITOR UTTON at the top of the window gives you quick access to file opera- tions, and column data.
  • Page 77 ACCQPrep HP125 Section 5 PeakTrak • I & T — Click this button to NITIAL ASTE INDOWS open a window from which you can view and modify these settings. For the Initial Waste, type or select the volume of eluent that is allowed to pass through the column to the waste port after the sample is injected.
  • Page 78 ACCQPrep HP125 Section 5 PeakTrak once for each gradient point that must be added to the plot area. • D —Click this button to enable the gradient point ELETE delete mode. When this mode is active, the system will delete the point nearest the next click on the gradient curve.
  • Page 79 ACCQPrep HP125 Section 5 PeakTrak • P — Click this button to collect only eluted peaks in EAKS the fraction collection tubes during a run. • N — Click this button to divert all fluids during a run to the waste port.
  • Page 80 ACCQPrep HP125 Section 5 PeakTrak value. The peak will end when the detector signal drops to .01 AU below the programmed value. This reduces the potential of multiple peaks if a noisy signal oscillates around the threshold value. Note If both Slope and Threshold peak detection methods are checked, the system considers a peak to be present when any one condition is met.
  • Page 81 ACCQPrep HP125 Section 5 PeakTrak · Monitor – Clicking on this option prevents fraction- ation based on the mass spectrometer signal. · Terminate On Target – stops the run after all mass spectrometer detection ions have been detected. · Detection Ions – used to set ions for detection or to be monitored.

  • Page 82: Column Data

    ACCQPrep HP125 Section 5 PeakTrak to 90 °C, but is limited to a range of 5 °C below and 60 °C above the ambient temperature. The drift tube temperature must be high enough to evaporate the solvent aerosol entering the drift tube. If the temper-...

  • Page 83: Set Data Path

    ACCQPrep HP125 Section 5 PeakTrak · N — Click this button to create and name OLDER a new folder. · U — Click this button to browse the contents of the next-higher folder level. · S — Enter a keyword and click the S...

  • Page 84: Ms Method Development

    ACCQPrep HP125 Section 5 PeakTrak • Current Data Path — The top-left corner of the window displays the path (current folder). As you browse through the files, the path will update as you go. • N — Click this button to add and name a subfolder within the currently selected folder.

  • Page 85: Ms Manual Control

    ACCQPrep HP125 Section 5 PeakTrak detected. The red spectrum is reset after injecting a sample. To inject a sample, move the PurIon injection valve to the “inject Sample position” • Inject the sample (>20 uL) and move the valve to the “Scan Mass”...

  • Page 86: Ionization Settings

    ACCQPrep HP125 Section 5 PeakTrak To prime the pump, open the priming valve on the fluid interface counterclockwise. Use the 5 mL Luer lock syringe provided with the accessory kit to draw liquid into the pump. Repeat this procedure once more to completely fill or purge the line from the carrier solvent reservoir to the priming valve.
  • Page 87 ACCQPrep HP125 Section 5 PeakTrak mass range even when the range is set within than the minimum and maximum values allowed for the spectrometer. Values can range from 50 to 1200 Da (PurIon and PurIon S) or 2000 Da (PurIon L). Changing...

  • Page 88: Manual Control

    ACCQPrep HP125 Section 5 PeakTrak • Offest - Offset is the voltage applied to all masses. Large values tend to increase fragmentation but reduce adduct formation. • Span - Span voltage defines an increased voltage applied as the mass increases. As with the offset, larger values increase fragmentation.

  • Page 89: Set Administrator Password

    ACCQPrep HP125 Section 5 PeakTrak • The “Static IP” option will require an IP address, Netmask, and Gateway provided by you network admin- istrator. The IP address, Netmask, and Gateway will be automatically retrieved from the DHCP server when selecting the DHCP option.

  • Page 90: Examples Of Peaktrak Actions

    ACCQPrep HP125 Section 5 PeakTrak 4. 4.Click the OK button to save your settings and close the window. The following menu commands will require a password before displaying the requested window: • T > U OOLS ANAGEMENT • H > S ERVICE •...
  • Page 91 ACCQPrep HP125 Section 5 PeakTrak Initial Waste & Time Windows – The initial waste diverts a user-determined volume to the waste port until the eluant is about to be collected. At the start of a run, the internal solvent lines hold some solvent from the previous run. This...
  • Page 92 ACCQPrep HP125 Section 5 PeakTrak Peak Detection – This section of the window contains option buttons to enable and disable various peak detection options. When an option is enabled, the window also allows you to modify the setting details for that peak detection option. All enabled options will be displayed on the chromatogram.
  • Page 93 ACCQPrep HP125 Section 5 PeakTrak enabled, click the D button to configure additional ETAILS settings. These settings include the slope-based and peak width options described in λ1 and λ2 above. Additionally, you can type or select the minimum and maximum wave- lengths limits in nanometers.

  • Page 94: Alternative Ways To Create Method Files

    ACCQPrep HP125 Section 5 PeakTrak • Detection Ions: Sets ions for detection or to be monitored. Up to 4 single ions may be chosen, or a range of ions and up to 3 single ions may be chosen. On PurIon S and PurIon L systems, detected ions may be a mixture of positive and negative ions.

  • Page 95: Real-Time Gradient Editing

    ACCQPrep HP125 Section 5 PeakTrak to add a point. You can then drag the new point to the desired location. Delete a point by clicking the D button and then ELETE clicking the undesired inflection point. The above methods work in the M...

  • Page 96: Monitoring The Purity Measure

    ACCQPrep HP125 Section 5 PeakTrak 5.3.6 Monitoring the Purity When using two wavelength detection options, a ratio of the two Measure wavelengths can also be displayed which at times can provide the best indication of compound purity. Refer to the following dis- cussion.
  • Page 97 ACCQPrep HP125 Section 5 PeakTrak view this information. On PurIon equipped systems, the mass spectrum of the fraction contents can be displayed by pushing the MS button and clicking on a tube.” • Method parameters — Click the D ISPLAY...
  • Page 98 ACCQPrep HP125 Section 5 PeakTrak • UV-visible spectrum (UV-Vis systems only). Clicking in the Chromatogram window displays a mass spectrum at that point during the run. • E button — Click the E button XTRACT ETHOD XTRACT to load a new method file based on the parameters for the run you are viewing.

  • Page 99: Manual Control Of The Accqprep

    ACCQPrep HP125 Section 5 PeakTrak 5.3.8 Manual Control of the Manual Control can assist with method development, mainte- ACCQPrep nance of the system plumbing, and system troubleshooting. To manually control the ACCQPrep system, first open the MANUAL CONTROL window by clicking the T >...
  • Page 100 ACCQPrep HP125 Section 5 PeakTrak 5-36...

  • Page 101: Section 6 Maintenance

    Section 6 Maintenance 6.1 Introduction This section will cover some common maintenance routines for the ACCQPrep HP125. 6.1.1 Cleaning To clean the exterior surfaces, use a cleaning cloth dampened with a mixture of distilled water and a mild detergent. Use iso- propyl alcohol for tougher stains.

  • Page 102: Tubing Inspection

    ACCQPrep HP125 Section 6 Maintenance cord can be unplugged, or use a power strip where the plug can quickly be removed from the outlet in the event of an emergency. When you first place the system in S , internal compo-...

  • Page 103: Preventive Maintenance

    ACCQPrep HP125 Section 6 Maintenance Vacuum or pressurized air applied to the outlet end of the drain tube must exist at the collection tray drain hole. Figure 6-1 Fraction collection tray drain hole 6.3 Preventive The system requires preventive maintenance for safe and reliable operation.

  • Page 104: Flow Cell Cleaning

    ACCQPrep HP125 Section 6 Maintenance 6.4 Flow Cell Cleaning 6.4.1 Post Separation As a preventive measure, all default column methods finish the separation run with a high percentage of solvent B (Figure 6-2). This brief time (one to six column volumes) of strong solvent flushes residual compounds from the column, flow cell, and internal tubing.

  • Page 105: Quick Cleaning When Recommended

    ACCQPrep HP125 Section 6 Maintenance 6.4.2 Quick Cleaning when When the lamp energy is lower than normal, the system will rec- Recommended ommend flow cell cleaning (Figure 6-3) before starting a sepa- ration run. When the system displays this message you can: •...

  • Page 106: Monthly Flow Cell Cleaning

    If the numerical values of the Raw Lamp Energy (Figure 6-3) do not change, or if the first number remains at zero, contact Teledyne ISCO’s Technical Service department. 6.4.3 Monthly Flow Cell Perform this procedure as part of your scheduled preventive...

  • Page 107: Accqprep Hp125 Maintenance

    EQUIRED and monitor the Raw Lamp Energy. (Figure 6-3) If the lamp energy is in the green range return the system to operation. If the lamp energy is red, contact Teledyne ISCO’s Technical Service department for assistance. 6.5 ACCQPrep HP125 Maintenance 6.5.1 Injection Valve Rotor...
  • Page 108 ACCQPrep HP125 Section 6 Maintenance Gap will appear here Screws Figure 6-5 Location of screws on the stator retainer 2. Remove the screws and pull the stator outward. This may require a side-to-side motion until removed. 3. When the stator is removed, use your finger or a small tool to pry the black plastic rotor out of its recess (Figure 6-6).

  • Page 109: Pump Seal Replacement

    ACCQPrep HP125 Section 6 Maintenance 5. Clean and inspect the stator sealing surface (which con- tacts the rotor) for scratches. If this part is scratched it may need replacement also. Installing the New 1. Place the new rotor into the recess with the Injection Valve Rotor passages facing outward.
  • Page 110 ACCQPrep HP125 Section 6 Maintenance Figure 6-9 Removing the fasteners 9. Carefully pull the pump head forward and off the guide pins. Pull straight and slowly to prevent damage to the pis- ton (Figure 6-10). Note The seal back-up washer may remain on the piston. Remove the washer from the piston if it did not stay in the pump head.
  • Page 111 ACCQPrep HP125 Section 6 Maintenance Figure 6-11 Removing the pump spacer block 11. Insert the flanged end of the seal insertion/removal tool into the seal cavity on the pump head. Tilt it slightly so that the flange is under the seal, and pull out the seal (Fig- ure 6-12).
  • Page 112 ACCQPrep HP125 Section 6 Maintenance Figure 6-13 Cleaning the piston 13. Place a replacement seal on the rod-shaped end of the seal insertion/removal tool so that the spring (energizer) is visi- ble when the seal is fully seated on the tool. Insert the tool into the pump head (Figure 6-14).
  • Page 113 ACCQPrep HP125 Section 6 Maintenance Figure 6-15 Reinstalling the pump spacer block 15. Place the seal back-up washer on the piston. Replace the pump head. Make sure that the inlet check valves are on the bottom and the outlet check valves are on top (Figure 6-16).

  • Page 114: Seal Conditioning

    The following instructions are for the removal and replacement Replacement of the pump check valves for the ACCQPrep HP125 system. 1. Turn the power O 2. Access the pump compartment by grabbing the edges of front panel and pulling forward on both sides (Figure 6-18).
  • Page 115 ACCQPrep HP125 Section 6 Maintenance 3. The solvent supply lines to the pumps have fluid in them that will be drained during this procedure. If it is accept- able to drain this fluid back into the supply bottles, follow step ‘a’ below, if not, go to step ‘b’.
  • Page 116 ACCQPrep HP125 Section 6 Maintenance Inlet lines Outlet lines Pump drive connector Figure 6-20 Pump assembly 7. Pull the pump assembly forward to the position shown in Figure 6-21. Figure 6-21 Pump assembly 8. With the pump assembly pulled out, remove the inlet fit- tings connected to the bottom of the pump head.
  • Page 117 ACCQPrep HP125 Section 6 Maintenance 9. Using a phillips screwdriver, remove the ‘Y’ fitting located above pump heads (Figure 6-23). Figure 6-23 Location of tee mounting screws 10.Using a ” wrench, remove the check valve housings from the top and bottom of the pump heads (Figure 6-24).

  • Page 118: Autosampler Maintenance

    ACCQPrep HP125 Section 6 Maintenance 6.6 AutoSampler The Peristaltic pump on the back of the AutoSampler may need to be replaced. Maintenance Peristaltic Tubing The peristaltic tubing P/N SP7477. Replacement Figure 6-25 Back of the AutoSampler 1. Removed the outer band of the peristaltic pump (Figure 6-26).

  • Page 119: Elsd Maintenance

    ACCQPrep HP125 Section 6 Maintenance 3. Slide the new peristaltic tubing with the thicker tubing towards the AutoSampler (Figure 6-28). Figure 6-28 Attaching the new tubing 4. Replace the outer band of the peristaltic pump (Figure 6-29). Figure 6-29 Replace the outer band 6.7 ELSD Maintenance...
  • Page 120 ACCQPrep HP125 Section 6 Maintenance Figure 6-30 ELSD Detection Options screens 5. Lift the p-trap drain tubing up to approximately case top level. Using a wash bottle, syringe, or suitable measuring device, slowly fill the drain line with up to 40 mL of ace- tone.
  • Page 121 ACCQPrep HP125 Section 6 Maintenance 6. Place the open end of the p-trap drain line in a 100 mL (or greater) beaker. Figure 6-32 Draining the acetone into a 100 mL beaker 7. Allow the acetone to drain out of the unit by dropping the end of the p-trap drain line, while it is in the 100 mL bea- ker, below the p-trap drain line level.

  • Page 122: Purion Maintenance

    ACCQPrep HP125 Section 6 Maintenance 6.8 PurIon Maintenance 6.8.1 ESI and APCI Removal Refer to Figure 6-33. from PurIon S and 1. Place the mass spectrometer in S mode. TANDBY PurIon L 2. Unscrew the ¼-28 PEEK fitting at the top of the ion source housing.

  • Page 123: Replacement Of Ion Source Housing

    ACCQPrep HP125 Section 6 Maintenance 6.8.4 Replacement of Ion 1. Place the ion source housing on top of the base plate and Source Housing line up with the rear electrical connection. Push down until source chamber is seated evenly across the base plate.

  • Page 124: Replacing Check Valves

    ACCQPrep HP125 Section 6 Maintenance Figure 6-34 Check valve holder 2. Remove check valves from pump head and place in a bea- ker of methanol. Sonicate check valves for 15 to 20 min- utes. 3. Reinstall check valves into pump head making sure that the ends of the check valves (with multiple holes) are fac- ing up towards the outlet of the pump.

  • Page 125: Capillary Inlet Cone Removal

    Even with the easy cleaning capability, Teledyne Isco recommends keeping a spare capillary inlet cone assembly (Teledyne ISCO part number 25-0000-085) to allow rapid replacement to minimize downtime while cleaning the plugged capillary.

  • Page 126: Capillary Inlet Cone Installation

    ACCQPrep HP125 Section 6 Maintenance 6.8.11 Capillary Inlet Cone 1. Ensure that the O ring seals are in position. Installation 2. Place the capillary inlet cone into the opening and press down until the threads are able to engage. There may be slight restriction in a downward motion as the part is almost completely inserted.
  • Page 127 ACCQPrep HP125 Section 6 Maintenance Table 6-1 Common PurIon Error Codes and Resolutions A plug has occurred in the PurIon fluid lines. The separa- This error message is displayed if a plug is detected dur- tion can be continued without PurIon detection or contin- ing a separation.
  • Page 128 ACCQPrep HP125 Section 6 Maintenance 6-28...

  • Page 129: Diagrams For The Autosampler And Accqprep

    ACCQPrep HP125 Appendix A A.1 Diagrams for the The following diagrams are for connecting the AutoSampler to the ACCQPrep system. These diagrams include: AutoSampler and ACCQPrep • A tubing diagram of the connections from the AutoS- ampler to the ACCQPrep (Figure A-1) •...
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  • Page 131 ACCQPrep HP125 Appendix A ACCQPrep HP125 Appendix A Auto Injector Optional Auto Auto External Sampler Sampler Detector Power Power Ethernet Column Valve To bottom Optional column port Waste Auto Auto Check Valve Auto Sampler Probe Sampler Sampler Wash Station Wash Pump 1 –...
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  • Page 133 ACCQPrep HP125 Appendix A Auto External Sampler Detector Power Auto Sampler Waste Level Sense Waste Solvent Inlet Main Power Solvent Level Sensing Drain Circuit Breaker Figure A-2 USB wiring diagram of the Autosampler connected to the ACCQPrep...
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  • Page 135 Appendix A ACCQPrep HP125 This page is intentionally left blank.
  • Page 136 This page is intentionally left blank.
  • Page 137 I, the undersigned, hereby declare that the design of the equipment specified above conforms to the above Directives and Standards as of July 17, 2017. Edward J. Carter USA Representative Edward J. Carter Director of Engineering Teledyne Isco, Inc. 4700 Superior Street Lincoln, Nebraska 68504 Phone: (402) 464-0231 Fax: (402) 464-3799...
  • Page 139 Name and amount of Hazardous Substances or Elements in the product Hazardous Substances or Elements Component Name (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) LCD Display Circuit boards Wiring Internal Cables Line Cord Stepper Motor Deuterium lamp Valve Body Name and amount of Hazardous Substances or Elements in the product O: Represent the concentration of the hazardous substance in this component’s any homogeneous pieces is lower than the ST/ standard limitation.
  • Page 141 NE 68504, U.S.A. *This warranty applies to the USA and countries where Teledyne does not have an authorized dealer. Customers in countries outside the USA, where Teledyne Isco has an authorized dealer, should contact their Teledyne dealer for warranty service.

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