Setting The Cross-Section To Be Observed - Olympus FLUOVIEW FV1000 User Manual

1-2-7-7 Setting the Cross-section to be Observed

While acquiring image, move the Z stage to select the cross-section to be observed.
1.
<Z stage fine adjustment> buttons
Displace the Z stage by 0.1 µm per
step.
2.
3.
Getting Started FLUOVIEW/
From the panel page tabs shown on the bottom right of the [Acquire] panel, select the
[Z Stage] sub-panel.
Fig. 1-8 [Z Stage] Sub-panel
Check the [Locked] check box in the [Z Stage] sub-panel.
Do not turn the fine focus adjustment knob while the [Locked] check box
is checked, for this may damage the Z motor.
While observing the image in the [Live] panel, locate the plane to be observed by
displacing the stage using the <Z stage coarse adjustment> and <Z stage fine
adjustment> buttons in the [Z Stage] sub-panel.
Outline of LSM Observation Procedures
[Current Pos] text box
Shows the current position of the stage.
The value can also be entered directly
from the keyboard.
<Z stage coarse adjustment>
buttons
Displace the Z stage by 1.0 µm
per step.
[Locked] check box
Z-motor is engaged by checking this
box.
OPERATION INSTRUCTIONS
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