Agilent Technologies 1100 Series Reference Manual page 49

In addition, the purity of a peak can be investigated by comparing spectra
obtained within a single peak. When a peak is calculated to be within the
user-defined purity limits, the purity factor is the mean purity value of all
spectra that are within the purity limits.
The reliability of the purity and the match factor depends on the quality of
spectra recorded. Because of the lower number of data points available with
the fluorescence detector in general, the match factors and purity data
obtained show stronger deviations compared to data from the diode array
detector, even if the compounds are identical.
Table 11 shows an automated library search based on the emission spectra
from a PNA reference sample.
Table 11 Peak confirmation using a fluorescence spectral library
Meas. Library CalTbl
RetTime
[min] [min] [min]
4.859 4.800 5.178
6.764 7.000 7.162
7.137 7.100 7.544
8.005 8.000 8.453
8.841 8.800 9.328
9.838 10.000 10.353
10.439 10.400 10.988
12.826 12.800 13.469
13.340 13.300 14.022
15.274 15.200 16.052
16.187 16.200 17.052
16.865 16.900 17.804
18.586 18.600 19.645
19.200 19.100 20.329
20.106 20.000 21.291
1100 Series FD Reference Manual
Signal Amount
[ng]
1 1.47986e-1
1 2.16156e-1
1 1.14864e-1
1 2.56635e-1
1 1.76064e-1
1 2.15360e-1
1 8.00754e-2
1 1.40764e-1
1 1.14082e-1
1 6.90434e-1
1 5.61791e-1
1 5.58070e-1
1 5.17430e-1
1 6.03334e-1
1 9.13648e-2
First Steps with the Fluorescence Detector
Purity # Match
Factor
- 1 993
- 1 998
- 1 995
- 1 969
- 1 993
- 1 997
- 1 1000
- 1 998
- 1 999
- 1 999
- 1 998
- 1 999
- 1 999
- 1 995
- 1 991
Libary Name
Naphthalene@em
Acenaphthene@em
Fluorene@em
Phenanthrene@em
Anthracene@em
Fluoranthene@em
Pyrene@em
Benz(a)anthracene@em
Chrysene@em
Benzo(b)fluoranthene@em
Benzo(k)fluoranthene@em
Benz(a)pyrene@em
Dibenz(a,h)anthracene@em
Benzo(g,h,i)perylene@em
Indeno(1,2,3-cd)pyrene@em
2
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