Operating Procedure - Nikon LABOPHOT-POL Instructions Manual

Polarizing microscope
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IV.
MICROSCOPY
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1) Turn
the brightness control
dial
(including
power switch) to light the lamp.
2) Bring the analyzer and the Bertrand lens out of
the optical path. (Refer to P. 12
&
13)
3) Place the specimen on the stage and swing the
10X objective into position. Focus on specimen.
4) Adjust the interpupillary distance and diopter.
(Refer to P. 8)
5) Place the filter on the field lens.
6) Carry out
the centering procedure for the
objective. (Refer to P. 8)
7) Carry out the centering procedure for the con-
denser. (Refer to P. 9)
8) Bring the analyzer into the optical path.
9) Swing in the objective to be used and refocus on
specimen.
10) Brightness is adjusted by changing the lamp
voltage.
Table 1
Orthoscopic
Conoscopic
y
microscopy
IN
higher
IN
4X or
OUT
wer
ertrand lens
OUT
IN
Circumscribed
70% ~ 80% of the
the circumfer-
cal aperture
ence of the
higher
of the objective
conoscoplC
4X or
field of view
opened
(or fully
opened)
scribed the
Circumscribed
circumference of
the circumfer-
higher
the eyepiece field
ence of the
of view
orthoscopic
Fully opened
field of view
10

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