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Thermo Scientific EASY-Spray ES081 User Manual
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Thermo Scientific EASY-Spray ES081 User Manual

Thermo Scientific EASY-Spray ES081 User Manual

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Thermo
EASY-Spray
Ion Source
User Guide
60053-97260 Revision A
May 2012

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Summary of Contents for Thermo Scientific EASY-Spray ES081

  • Page 1 Thermo EASY-Spray Ion Source User Guide 60053-97260 Revision A May 2012...
  • Page 2 © 2012 Thermo Fisher Scientific Inc. All rights reserved. Acclaim, EASY-Column, EASY-nLC, EASY-Spray, Exactive, Foundation, nanoViper, PepMap, Q Exactive, Velos Pro, and Viper are trademarks, and Dionex, LCQ, LTQ, Orbitrap, Thermo Scientific, UltiMate, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.
  • Page 3 Selecting Nanospray as the Ion Source ....... 31 Configuring the nanoESI (NSI) Source Parameters ..... . 32 Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 4 Index ..............45 EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 5 Controller PCB removed from the EASY-Spray source....36 Figure 27. Bottom of the EASY-Spray source without the controller PCB... . . 37 Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 7 Regulatory Compliance Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name. Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
  • Page 8 Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument. Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment.
  • Page 9 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 10 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 11 Preface The EASY-Spray Ion Source User Guide describes the hardware components, and provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™ ion source. Use the EASY-Spray ion source with the following Thermo Scientific mass spectrometers (MSs) for nanoelectrospray (commonly referred to as “nanoES” or “nanospray”) analysis: •...
  • Page 12 Environment” chapter in the preinstallation requirements guide for your Thermo Scientific mass spectrometer. CAUTION The EASY-Spray source must connect to a certified Thermo Scientific mass spectrometer, which supplies high voltage capable of delivering a maximum of 8 kV and 100 μA.
  • Page 13 1 set SC901 with three attached ferrules and nuts Sleeves, PEEK™, 1/16 in. OD IDEX™, F-233 To order another EASY-Spray source housing, you must order the EASY-Spray Ion Source Kit (P/N ES081) Thermo Scientific EASY-Spray Ion Source User Guide xiii...
  • Page 14 Hot Surface: Allow heated components to cool before touching or servicing the instrument. Trip Hazard: The power supply cords might present a trip hazard. Be aware of the cords’ locations and secure them in place as needed. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 15 • For countries other than the U.S., when you dial from within the specified country, dial the 0 of the city code. • For countries other than Italy, when you dial from outside the country, do not dial the 0 of the city code. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 16 ES-28108 Alcobendas - Madrid Phone ......[45] (70) 23 62 67 E-mail: service.sid.spain@thermofisher.com Fax......[45] (70) 23 62 63 Phone......[34] (914) 84 59 65 Fax ......[34] (914) 84 35 98 EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 17 E-mail: service.sid.hemel@thermofisher.com Phone (free lines)..800 810 5118 Phone ....... [44] (0) 870 241 1034 ........400 650 5118 Fax......[44] (0) 144 223 3667 Fax ......[86] 108 837 0548 Thermo Scientific EASY-Spray Ion Source User Guide xvii...
  • Page 19 Introduction The Thermo Scientific EASY-Spray ion source, shown in Figure 1, maintains excellent spray stability to ensure efficient evaporation and ionization of the liquid samples—the key to achieving the highest sensitivity at nano-flow rates. Key benefits of the EASY-Spray source are as follows: •...
  • Page 20 Note The Thermo Tune Plus application uses the terms “nanospray” and “NSI.” Functional Description This section describes the following principal components of the EASY-Spray source and their functions: • Source Housing • Controller PCB • Controller Power Supply EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 21 11) with the mass spectrometer’s ion transfer tube. To align the tool’s x-axis (side-to-side) and y-axis position (up-and-down), use the controls that are built into the EASY-Spray source housing. For instructions, see “Adjusting the Emitter Tip Position” page Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 22 “Heater.” The temperature control dial has an OFF setting and temperature settings in 5 °C increments for the 30–60 °C range. As the column heats up, the actual temperature appears on the display. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 23 The total length of the output cord and power adapter cable is approximately 9 ft (274 cm). Make sure that there is an electrical wall outlet within reach for the power supply unit and that you provide suitable clearance around it for cooling. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 25 Contents • Installing the Ion Source onto the Mass Spectrometer • Connecting the Controller Power Supply • Adjusting the Emitter Tip Position • Removing the EASY-Spray Source Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 26 The spray cone is directly behind the ion sweep cone. Figure 5. Ion sweep cone removed from the mass spectrometer’s spray cone Ion sweep cone Spray cone (shown is the offset orifice type for ESI, H-ESI, and APCI) EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 27 Figure 7. Ion source housing connection High voltage (HV) connector Guide pins on the mass spectrometer’s Guide pin holes on the back of the ion source mount assembly EASY-Spray source housing Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 28 Red dot on the socket Red dot on the plug b. Push the plug upward into the socket. CAUTION After completing the power supply connections, route the output cord so that it is not a trip hazard. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 29 Emitter positioning tool Mass spectrometer spray cone EASY-Spray column 3 mm gap between the ion transfer tube and an actual emitter (shown without its cable) Ion transfer tube Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 30 (Figure 11). Figure 11. X- and z-axes position controls on the EASY-Spray source Inserting the emitter positioning tool Z-axis position control (knob) X-axis position control (hex socket-head screw) EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 31 6. After you adjust the tool tip’s position, slowly remove the emitter positioning tool. The position of the column holder is now calibrated for use with any EASY-Spray column. You must repeat this procedure if you later change the x-, y-, or z-axis position. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 32 8. Grasp the source housing with both hands and slowly pull away from the mass spectrometer until it clears the guide pins. After you remove the EASY-Spray source from the mass spectrometer, place it in its original shipping box. The EASY-Spray source does not require cleaning. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 33 Using the 1.5 mm hex key, tighten the hex socket-head screw on the left side of the top observation window (Figure 11 page 12) until it touches the camera—do not overtighten the screw. c. Connect the camera’s black power connector to its power supply unit. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 34 5. Place the monitor on top of the mass spectrometer near the source by using its support arm. Make sure that the monitor is not too close to the edge. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 35 3. If the picture is too dark, turn on the camera light by pressing its top light button (Figure 13 page 16). 4. Adjust the picture by using the camera’s focusing wheel (Figure 13 page 16). Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 37 Connecting the LC to the EASY-Spray Column Follow these procedures to connect the plumbing from the Thermo Scientific LC instrument to the EASY-Spray column. Contents • Tools and Supplies • Installing the EASY-Spray Column for the First Time • Using the nanoViper Fitting •...
  • Page 38 This image shows the Acclaim™ (For the PepMap100 PepMap100 Nano-Trap. Nano-Trap) Fisher Scientific, 164705 Viper™ union Thermo Scientific Dionex™, (for use with the EASY-nLC 1000) 6040.2304 ZDV adapter union (1/16 to 1/32 in.) IDEX, P-881 and plug (not shown) (for use with the EASY-nLC II) —...
  • Page 39 4. Connect the cable to the front output heater socket. Note Do not pull on the cable from the EASY-Spray column. Use the plug to connect and disconnect the column. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 40 2. Using the black knurled fitting tool, tighten the screw clockwise to an angle of 0–45 degrees (1/8-turn). 3. Start operating the system at the desired working pressure and check the backpressure. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 41 Note Remove the nuts and ferrules from the venting tee. • To plumb a one-column setup from an EASY-nLC 1000 instrument • To plumb a two-column setup from an EASY-nLC 1000 instrument page Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 42 3. Connect the venting tee to the EASY-Spray column to complete the one-column setup (Figure 17). Figure 17. Example of a one-column setup with nanoViper fittings Column Out line EASY-Spray column Waste In line EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 43 When the One Column Setup check box is clear (not selected), valve W on the EASY-nLC 1000 moves to position 1–6 during the sample loading step. Excess solvent A exits the venting tee and passes through valve W to the waste beaker. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 44 4. Connect the venting tee to the EASY-Spray column to complete the one-column setup. Figure 17 page 24 shows an example of a one-column setup for the EASY-nLC 1000 instrument, which uses nanoViper fittings. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 45 1/4 in. wrench to tighten the nut until firmly secured—do not overtighten. 3. Connect the venting tee to the EASY-Spray column to complete the two-column setup (Figure 21). Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 46 Follow the appropriate procedure for a one- or two-column setup. The two-column setup includes a precolumn connected across the autosampler valve. Note To connect the RSLCnano system to the EASY-Spray column, you must use the Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY-Spray Application Kit (P/N 6720.0395). •...
  • Page 47 3. Connect the union to the EASY-Spray column to complete the one-column setup (Figure 22). Figure 22. Example of a one-column setup for the RSLCnano system (direct injection) EASY-Spray column 20 μm ID × 65 cm Viper union Autosampler 20 μm ID 1 μL loop Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 48 4. Connect the union to the EASY-Spray column to complete the two-column setup (Figure 23). Figure 23. Example of a two-column setup for the RSLCnano system (preconcentration) EASY-Spray column Viper union 20 μm ID Column compartment with two switching 20 μm ID valves Precolumn EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 49 Configuring the Mass Spectrometer for NSI Mode Follow the applicable procedures to configure the Thermo Scientific mass spectrometer for nanoelectrospray ionization (nanoESI or NSI) mode. Contents • Selecting Nanospray as the Ion Source • Configuring the nanoESI (NSI) Source Parameters...
  • Page 50 • In the Xcalibur application, click the Roadmap View icon, the Instrument Setup icon, and the MS icon. Then, from the main toolbar choose > Start Tune Plus. Figure 24. Tune Plus window for an NSI-configured Velos Pro (example) Icon for the NSI source EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 51 Use 1.90 kV as the start value for the spray voltage. If the intensity of the full-scan spectrum is low, gradually increase the spray voltage to improve the spectrum. The recommended range for the spray voltage is 1.40–2.40 kV. 4. Click OK. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 53 Tools and Supplies Table 6 lists the required tools and supplies for the maintenance of the EASY-Spray source. Table 6. Tools and supplies Description Part number Controller PCB and 2.5 mm hex key ES230 Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 54 4. Unpack the new controller PCB and make sure it is not damaged. CAUTION To prevent damage to the electronic components on the PCB due to ESD, hold the new controller unit by the metal enclosure instead of the PCB. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 55 6. Using the 2.5 mm hex key, install the four hex socket-head screws into the bottom of the controller PCB enclosure. You can now install the EASY-Spray source back onto the mass spectrometer. To dispose of the old controller PCB, see “WEEE Compliance” page Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 57 Try adjusting the spray voltage. If that does not resolve the from particles in the sample, blockage problem, insert a new EASY-Spray column. other small particles from the flow lines or valves, and so on. Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 58 –or– EASY-Spray source. The actual • See “Replacing the Controller PCB” page temperature The EASY-Spray column is Replace the EASY-Spray column. display is blank. damaged. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 59 EASY-Spray column, 25 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles ..ES802 EASY-Spray column, 50 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles ..ES803 Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 61 1–20 nL/min (for static nanoelectrospray) or ionization technique available to transform ions in 100–1000 nL/min (for dynamic nanoelectrospray, solution into ions in the gas phase. which is also called nanoESI nanoLC gradient separation). Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 62 NSI spray current varies. nanospray ionization (NSI) See nanoelectrospray ionization (nanoESI or NSI). source See source. static nanoelectrospray A device that performs continuous analysis of small analyte solution volumes over an extended period of time. EASY-Spray Ion Source User Guide Thermo Scientific...
  • Page 63 EASY-Spray ion source power connection UltiMate 3000 RSLCnano EASY-Spray Application removing x- and z-axes controls y-axis control electrical wall outlets electromagnetic compatibility EMC compliance Thermo Scientific EASY-Spray Ion Source User Guide...
  • Page 64 NSI mode manual configuration, LCQ Deca XP Max mass spectrometer setting the spray voltage NSI Source dialog box power connection, location power supply units, connecting camera controller monitor product requirements regulatory compliance replaceable parts EASY-Spray Ion Source User Guide Thermo Scientific...