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1200 SERIES SPECTROPHOTOMETER USER’S MANUAL United Products & Instruments, Inc. 182 Ridge Road, Suite E Dayton, NJ 08810, USA TEL: 732-274-1155 FAX: 732-274-1151 www.unicosci.com sales@unicosci.com...
General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this spectrophotometer it is essential for laboratory personnel to follow generally accepted safe procedures in addition to the safety precautions called for in this manual.
Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity, ® it is necessary that only shielded cables supplied by UNICO are used when connecting the instrument to computers and accessories. Introduction ® UNICO 1200/1201 Spectrophotometers are single beam, general purpose instruments ®...
Model 1200 Model 1201 Wavelength Range 325~1000 nm Spectral Bandpass 5 nm Wavelength Accuracy ± 2 nm Wavelength Repeatability ± 1 nm Stray Radiant Energy < 0.5%T at 340 and 400 nm Photometric Range 0~125%T 0~2.0 Abs 0~1999C (0~1999 Factor) Photometric Accuracy ±...
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Mode 0A/100%T Print/Enter Sample compartment Wavelength Knob Figure-3 UNICO ® 1200 Spectrophotometer MODE Button: There are four modes. T mode is transmittance mode; A mode is absorbance mode; C mode is to measure unknown sample concentration through a standard solution;...
Turn on the spectrophotometer by turning on the Power Switch (IO). Allow 15 minutes for the instrument to warm up. ® Even through the 0%T is pre-set at UNICO ; it may shift due to unexpected factors like temperature change. To check 0%T, simply insert the Black Block (item number: S-1200-111, used to check the 0%T) into the Cuvette Holder and close the lid.
follow step 2 above to set the concentration of the standard to the reduced value. 8. With the standard concentration set, determine the concentration values of samples with unknown concentration by inserting the sample cuvette into the Sample Compartment and reading the value direct from the Digital Display. 9.
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3. Loosen-up 2 lamp holding screws pointed by arrows on the image below. Remove damaged bulb and insert the new bulb all the way in to the holder. Lightly tighten the holding screws but not all the way. 4. Plug the power cord back and turn the instrument on. Set the wavelength to 340nm. Avoid to stair directly on to the bulb.
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Wavelength Calibration Check Normally the UNICO ® 1200 Series spectrophotometer retains its wavelength calibration indefinitely. However if the instrument receives a severe shock or is abused, use the following methods to check wavelength calibration. Please note that this test requires ®...
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It should be within 527~531 nm. Absorbance Accuracy Check Specification: ± 1% at 1A The absorbance accuracy should be checked against a set of neutral density filters ® accurately calibrated to the NIST standards. Contact your UNICO representative for more information (800-588-9776 or 732-274-1155).
Specification: Less than 0.5%T at 340 nm by ASTM E 387 The stray light should be checked against a set of filters accurately calibrated to the ® NIST standards. Contact your UNICO representative for more information (800-588- 9776 or 732-274-1155).
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Bubbles or particles in solution Check sample preparation and analytical procedure Instrument out of electronic calibration Call an authorized service engineer UNICO Authorized Service Contact Information UNICO 182 Ridge Road, Suite E, Dayton, NJ 08810, USA Tel.: 800-588-9776 or 732-274-1155 Fax: 732-274-1151 Web: www.unicosci.com...
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