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2100 SERIES SPECTROPHOTOMETER USER’S MANUAL United Products & Instruments, Inc. 182 Ridge Road, Suite E Dayton, NJ 08810, USA TEL: 732-274-1155 FAX: 732-274-1151 www.unicosci.com...
General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual.
Introduction The UNICO Model’s 2100 and 2100UV Spectrophotometers are single beam, general purpose ® instruments designed to meet the needs of the conventional laboratory. UNICO ® 2100 and 2100UV are ideal for various applications, such as: Clinical Chemistry, Biochemistry, Petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control.
to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display. Specifications Table-1 lists the specification for both Model 2100 and 2100UV. Table-1 Specifications Model 2100 Model 2100UV Wavelength Range 325~1000 nm 200~1000 nm Spectral Bandpass...
4. Select either 230V or 125V/115V on the voltage selector switch on the back of the 2100 and 2100UV shown in Figure-2, to match your local main voltage supply. 5. Turn on your UNICO 2100 and 2100UV and allow it to warm up for 15 minutes before ®...
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If using UNICO ® Windows-based Application Software, press this button to communicate with the software (refer to the UNICO ® 2100 Series Spectrophotometer Software User’s Manual Version SB-2.10 for details). If operating in F Mode, then press to enter factor. This causes the Mode to enter the factor number and change to C mode.
Figure-3 Model 2100UV Spectrophotometer Figure-4 Operation Panel of Model 2100UV Model 2100 Operation Panel The Model 2100 has the similar outlook as Model 2100UV. It also has the following components:...
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If using UNICO ® Windows-based Application Software, press this button to communicate with the software (refer to the UNICO ® 2100 Series Spectrophotometer Software User’s Manual Version SB-2.10 for details). If operating in F Mode, then press to enter factor. This...
Each step is displayed on the Wavelength Display Screen. Figure-6 to Figure 12 and Table-3 show the detailed information of the Start-up Self-Calibration: P1 Process, P2 Process, P3 Process, P4 Process, Show UNICO Process, PC CONN Process, and Normal Start-up Screen. Figure-6...
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Monochromator locate “0” order light and Monochromator locate “0” order light, and measure initial light energy measure initial light energy • • Monochromator go to 546 nm Monochromator goes to 546 nm • • Instrument sets 0%T Instrument sets 0%T. •...
Figure-10 Show UNICO Process Figure-11 PC CONN Process Figure-12 Normal Start-up Screen Changing Cuvette Holders There are seven different Cuvette Holders (Sample Holders) (refer to Figure-13 and Figure- 14, from left to right): • S-2100-101P: Test Tube Holder for 8~20 mm diameter test tubes. Includes Universal Base, one holder, and Top Hat for up to 150 mm tall test tubes •...
temperatures from 0~95°C. Includes Holder, Universal Base, and Flowthru Panel (Water Bath Required) • S-2100-106P: Micro-Cuvette Holder Package for use with 10 mm microcuvettes. Includes adjustable x-y base and holder • S-2100-109P: Peltier Flowcell Package for 25, 30 and 37°C. Includes p/n S-2100-109, peristaltic temperature/pump/flow-thru controller, p/n S-2100-107A, Peltier Thermal Electric Cuvette Holder and peltier/flow-thru Refer to the Attachment in this Manual for the detailed Installation Guide.
Select either the T (Transmittance Mode) or A (Absorbance Mode) by pressing the MODE button until the red light for T or A is on. Select the desired wavelength by pressing the appropriate Wavelength Control buttons ∧ and ∨. At any time if the wavelength is changed, the Data Display Screen will display “BLA”.
“frozen”. This requires the operator to re-start at step 1. Output and Data Processing Printer Model 2100 and 2100UV have a RS232C Interface connector that can be connected to any RS232 printers (UNICO ® provides RS232 printer, p/n: S-1100-206, as an optional accessory). It...
Absorbance vs. Time Kinetics: measure a sample’s absorbance change over a selected period of time, store the test results in data table, and display the results graphically. Refer to the UNICO ® 2100 Series Spectrophotometer Software User’s Manual Version SB-2.10 for detail. Maintenance Lamp Replacement Replace Tungsten-Halogen Lamp 1.
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6. Remove the lamp by disconnecting the 3-wire connector and pulling straight up on the lamp socket. DO NOT PULL on the lamp itself. This may cause the lamp to break. 7. Replace the pre-aligned lamp with a lamp provided by UNICO ®...
2100 Series spectrophotometer retains its wavelength calibration indefinitely. However if the instrument receives a severe shock or is abused, use the following methods to check wavelength calibration. Please note that this test requires the UNICO ® Didymium filter, p/n S-2100-116, or the Holmium Oxide filter, p/n S-2100-115.
Absorbance Accuracy Check Specification: ± 0.004A at 0.5A, ± 1% at 2A. The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards. Contact your UNICO ® representative for more information (1-800-588-9776).
Table-4 2100 an d 2100UV Parts List Catalog # Description S-2100 UNICO Model 2100 Spectrophotometer 5 nm Bandpass ® Wavelength range: 325~1000 nm, automatic wavelength change, Voltage preset at 110V Complete with 4-position Cuvette Holder, 4 matched Optical Glass Cuvettes...
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S-2100-104 Cylindrical Cuvette Holder without base (Requires Universal Base) S-2100-105 Water-Jacketed Single Cuvette Holder with flow-thru panel but without base (Requires Universal Base, Water Bath) S-2100-107A Peltier Thermal Electric 10 mm Cuvette Holder with panel S-2100-107B Peltier controller unit for controlling temperature from 15 to 40°C S-2100-108A Flow-thru panel for use with ambient flowcell system (requires flowcell, tubing and p/n S-2100-109 flow- thru/peltier controller...
Troubleshooting Table-5 Troubleshooting PROBLEM Possible Cause Solution Instrument Power cord not connected to outlet Plug instrument in Inoperative Dead Power outlet Change to a different outlet (Power Wrong voltage setting indicator has Internal fuse blown or defective electronic Call an authorized service no light) component engineer...
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Troubleshooting continued PROBLEM Possible Cause Solution Digital Display Concentration reading “frozen” MODE switch has been does not change changed from C to F, T or A and regardless of back to C Restart measurement sample procedure concentration Wrong wavelength setting Check sample procedure and wavelength setting Insufficient sample volume...
Troubleshooting continued PROBLEM Possible Cause Solution Incorrect readings Insufficient sample volume Fill cuvette with more samples obtained Wrong wavelength setting Check analytical procedure and Failed to blank (0A/100%T) wavelength setting Check wavelength Failed to set 0%T accuracy according to procedure in this manual Stray sample preparation vapors Prepare sample away from instrument.
ERR 03 Instrument unable to locate “0” order • Monochromator contact switch or grating- light ( 2100 and 2100UV); or driven motor is malfunctioning (2100UV) monochromator unable to locate • Check tungsten-halogen lamp. Replace lamp if starting position (2100UV) necessary (both) •...
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Step 2 The only tool needed is a standard Philips head screwdriver. Step 3 Remove the 4-position Cuvette Holder: Open the Sample Compartment cover and remove any cuvettes or any breakable items. Push in the Cuvette Holder Control Knob (black knob) all the way back (First position is into the light beam).
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from holder. Gently slide the guide rod out of the Sample Compartment. Step 5 Looking over the Sample Compartment, remove the front screw (Figure-15). Step 6 To remove the back screw, place your hand ON TOP of the 4-position Cuvette Holder and gently slide towards the front.
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Micro-Cuvette Holder Package Accessory Installation Guide for (S-2100-106P) Step 1 Remove all items from the accessory package made sure that they are complete (refer to Figure-14 for the S-2100-106P): A Cuvette Holder with X-Y adjustable bas 1 pc Installation Guide 1 pc Step 2 The only tool needed is a standard Philips head screwdriver.
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Tighten the rear screw. It may not be necessary to replace the front screw since the alignment pins will properly place the base. Adjusting the Micro-Cuvette Holder Step 8 Please turn on the power to the instrument if the unit is off. After self-calibration, the spectrophotometer will stop at 546 nm (green light) Step 9 Place your micro-cell cuvette into the cuvette holder.
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Figure-18 Micro-Cuvette Holder Front View Figure-19 Micro-Cuvette Holder Right-side View (Note: Light beam guide is eliminated from latest design change) Peltier/Flowcell Accessory Installation Guide (S-2100-109P) Step 1 Check all items from the accessory package and make sure they are complete (refer to Figure-14 for S-2100-109P): Peltier/Flowcell Controller with peristaltic pump 1 unit...
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AA) Horizontal setting: There is one knob located in front-right of the Cuvette Holder base for horizontal (backward or forward) adjustment. Turning this knob clockwise will “push” the Cuvette Holder backward and counterclockwise will “push” the Cuvette Holder toward you. Turn on the spectrophotometer and MONITOR the transmittance reading on the display.
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positions on both the Front and Back side of the Peristaltic Pump. Figure -22 Peltier/Flowcell Controller Side View Step 14 The external components are ready. Now, with tubing supplied by either the manufacturer or the user, connect the flowcell to the Flow-Thru Panel. Two different tubing will be needed inside the Sample Compartment: Inlet tubing: from inlet connector (pipe) to the flowcell.
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Figure-24 Peltier/Flowcell Accessory Front View Step 16 Press the Mode button on the Controller. The number showing is the number of seconds the peristaltic pump on the flowcell system will operate. To adjust this, use the “up” and “down” buttons. PLEASE NOTE: Since sample size and desired volume varies from sample to sample, the user must experiment to find the optimum pump time.
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