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- Page 1 S1205 SERIES SPECTROPHOTOMETER USER’S MANUAL V 3.0 United Products & Instruments, Inc. 182-E Ridge Road Dayton, NJ 08810 732-274-1155 FAX 732-274-1151 TABLE OF CONTENTS...
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Page 2: Table Of Contents
General information……………………………………………………………… Safety…………………………............……… Electrical………………………………………………………………..Warning…………………………………………………………………….. Performance………………………………………………………………… Radio Interference………………………………………………………….. Introduction…................……………… Working Principle...............…………… Unpacking Instructions.............………………….. Specifications…....................Installation................……....Operational Panel..............……....Description of Keys..............……................……………....Operation Instruction Prepare the spectrophotometer................. BASIC MODE-%T/Abs Measure..........……………. Quantitative Test...............……………… Run test……………………………………………………………….. Define/Establish Test Method……………………………………….. By Known Factors……………………………………………. By Standard………………………………………………….. UTILITY………………………………………………………………………... -
Page 3: Safety
The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this apparatus it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual. -
Page 4: Performance
Introduction The UNICO S1205 is single beam, general purpose instruments designed to meet the needs of the Conventional Laboratory, They are ideal for various applications, such as: Clinical Chemistry, Biochemistry, Petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control and research. -
Page 5: Unpacking Instructions
The S1205 incorporate an RS-232C for printing and a USB port for connecting to a PC for using the UNICO Application Software. There is a function select switch above the USB and RS-232 ports. -
Page 6: Installation
Photometric Accuracy + 0.004@0.5A Data output RS232 and USB Power Requirements 80-240Vac, 50-60 Hz Dimensions 420W x 340D x 180H (mm) Light Source Tungsten Halogen Weight 8.5kg Shipping Weight 19 lbs Shipping dimension 20 x 16 x 11 inches Installation: 1. -
Page 7: Operation Instruction
【CLEAR】 Clear number or character when editing in Quantitative Mode; 【LOAD】 Load saved data or saved curve ; 【PRINT】 Print data; 【SAVE】 Save data or curve; 【100%T】 Set 0Abs and 100%T; 【EXIT】 Return to previous screen; 【ENTER】 Confirm the inputted data or selected item; Go to next setup or screen; 【... -
Page 8: Basic Mode-%T/Abs Measure
2. Dark Current will be taken shown and be saved shown after pressing EXIT Important Notes: It is always a good practice to reset the dark current periodically. It is especially important if the instrument is hanging on for a long time or the environment is not stable. - Page 9 【 ∧ 】,【 ∨ 】 Select wavelength using Press Enter to set the new wavelength Blank automatically Make a blank reference solution by filling a clean cuvette with distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid.
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Page 10: Quantitative Test
position by pulling on the sample holder rod until the holder “click” into place. Be sure to make note of the readout (or print) for each sample. Remove the sample cuvette(s). Operational Note: 1. If you are reading 3 or less samples, then place the reference cuvette in the position nearest you, and the samples in the next available position. -
Page 11: Run Test
The instruments will automatically set to the proper wavelength as defined and blank the reference(Press 0Abs/100%T button to re-blank). Goto preset wavelength Blank automatically Ready after Blanking 3. Insert the sample cuvette into the light-path (working position) and sample test result will be displayed. -
Page 12: Define/Establish Test Method
2. Press CLEAR to remove it. (Note: The test method is still saved in All Test List and can be reloaded as needed). Define/Establish Test Method Test method (curve) must be defined and established before quantitative tests can be run. This instrument has open platform for you to establish your own test methods (curves). - Page 13 Set wavelength by pressing 【 ∧ 】,【 ∨ 】. Press Enter to confirm wavelength selection (581nm in this example case). The screen returns to previous menu and the * move to “Conc. Unit” automatically. Select concentration unit by pressing 【∧】,【∨】. Press Enter to confirm Conc. Unit selection.
- Page 14 Use CLEAR to delete/erase any incorrect entry. Press 【∨】to alter the polarity of the number. Check and make sure the intercept value is correct. Then press ENTER to confirm and move to the next screen as displayed. Enter the slope value in the same way as the intercept value is entered. The Slope value in this example is 2.588.
- Page 15 Save the Curve defined: with the * at “Save Curve” press ENTER to save the define curve. Then the * moves to “Dis & Print Curve” automatically. 10. Display and Print-out Defined Test (Curve parameter): with the * at “Dis & Print Curve” Press ENTER to display and print (if printer is connected) the defined test parameter.
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Page 16: By Standard
Select “By Standard” by pressing ENTER button. Follow the screen step by step to enter data and parameters. Set wavelength by pressing 【 ∧ 】,【 ∨ 】. Press Enter to confirm wavelength selection. The screen returns to previous menu and the * move to “Conc. Unit” automatically. Select concentration unit by pressing【∧】,【∨】. - Page 17 1st order linear: Concentration = Intercept+ Slope*Absorbance; order linear through zero: Concentration = Slope*Absorbance; Press Enter to confirm fitness method selection. The screen returns to previous menu and the * move to “Measure Standard” automatically. Important Note: At least two standard solutions are required to establish 1 order linear through zero curve.
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Page 18: Utility
If the “No. of Replics” is “3”, then place the second solution of STD No.1 in the cuvette holder. Pressing ENTER will measure the second solution of STD N0.1 and the second measurement value will be displayed at STD No.1= 0.18775A2. Repeat the same procedures to measure the third solution of STD No.1 and screen will display STD. -
Page 19: Wl Calibration
There are eight functions in UTILITY. WLCalibration: the instrument will implement a self-check and calibration procedure. It includes filter positioning, lamp positioning, and wavelength calibrating, dark current resetting; Dark Current: measure and refresh instrument dark current. It is designed for eliminating the error from electronic drifting. -
Page 20: System Baseline
2. Display of Date or Time The instrument screen can either display date or time (hour:min:sec). Select Display Mode in Fig. 30. Press 【∧】【∨】to select Display Time or Display Date. Press ENTER to confirm. Press EXIT to return to Edit Clock screen. 3. -
Page 21: Reset Defaults
Reset Defaults: Reserved for future application; Memory Manager: It has three functions as shown. A. Load Saved data Select Load Saved data. The saved data will all be listed. With * next to the saved data interested (COBALT in this case), press ENTER, you can print the data (if printer installed) and display it on screen, or you can send the data to computer . -
Page 22: Pc Link
In Fig. 7, move * to the left of PC LINK and press ENTER; the following screen is displayed. When the communication between the instrument and the computer is established the computer is in control. Please details of the PC software please refer to UNICO application software manual. Wavelength Calibration: Normally the spectrophotometer retains its wavelength calibration indefinitely. -
Page 23: Holmium Oxide Filter Method
Oxide filter. In the filter method, the didymium filter has two distinct absorbance peaks at 529nm and 807nm. The Holmium filter has a distinct peak at 361nm. When the instrument is calibrated properly you will find minimum Transmittance (maximum Absorbance) at the range +2nm from these peaks. -
Page 24: Absorbance Accuracy Checks
The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards. Contact your UNICO representative for more information (800-588-9776). An alternative method using potassium dichromate is described below. Due to the many factors that might affect the results (i.e. -
Page 25: Stary Light Check
Note:It is recommended that you refresh the Dark Current (refer to Page21) before check. Stray Light Check Specification: Less than 0.3%T at 340nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows: Set the wavelength to 340nm. -
Page 26: Error Codes Definitions
Instrument drift and Lamp not adjusted Check lamp has been properly installed or properly.(misalignment) has moved during transit. noise Lamp old or defective. Replace with a new lamp. Defective or dirty detector or Call an authorized service engineer. defective electronic component. Insufficient sample volume Fill cuvette with more sample. - Page 27 • Instrument unable to Error 1 Check power supply to see if proper voltage is initialize and/or locate the selected. • lamp (S2100UV+only). Open cover of instrument and check the connector J3 between CPU board and Lamp-change-over motor . • Lamp-change-over motor or lamp initialization switch malfunctioning,Check the connector J8 on CPU board.
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