Genevac HT-12 User Manual
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Genevac HT-12 User Manual

Evaporation systems
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Summary of Contents for Genevac HT-12

  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
  • Page 2 & & Issue 1-9 – June 2008 Part Number 04-4541 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 4: Table Of Contents

    Genevac HT-8 & HT-12 Series II Evaporating Systems Contents Introduction ............................5 Amendment Control Form ........................6 Safety symbols ............................. 6 Genevac Evaporators and Combustible Solvents ..................... 6 System description and options ....................... 7 Scope of delivery and installation ..................... 8 Checking the delivery ...........................
  • Page 5 Genevac Limited. All rights reserved. © Genevac Limited These operating instructions should be read before you use the Genevac HT-8 & HT-12 Evaporating System. Keep them near the system for easy reference. Your attention is drawn in particular to Section 4 Safety.

  • Page 6: Introduction

    Genevac HT-8 & HT-12 Series II Evaporating Systems Introduction The Genevac Series II range of evaporation systems are state-of-the art and represent a significant step forward in evaporation technology for the R&D laboratory. Drawing on extensive experience in the drug...

  • Page 7: Amendment Control Form

    Please note it remains the responsibility of the user to consider safety when evaporating any combustible solvents and ensure the system is placed in a well ventilated environment. Genevac's position regarding evaporation of such solvents, particularly with respect to the European ATEX directive, is available on our website or from your local sales representative.

  • Page 8: System Description And Options

    Genevac HT-8 & HT-12 Series II Evaporating Systems System description and options Your HT-8 and/or HT-12 Evaporation System is comprised of an evaporation chamber and rotor, with an external a cryopump-condenser unit. Vacuum is provided by the Scroll Pump, although other types of vacuum pumps can be used.

  • Page 9: Scope Of Delivery And Installation

    Genevac Ltd immediately of any missing or damaged parts. (Refer to section 10 for contact details). Arranging commissioning If your system is to be delivered separately, Genevac Ltd will contact you prior to the delivery, to agree a date to commission your system. Training Commissioning will normally include training in the basic operation of the System.

  • Page 10: Fitting The Vacuum Pump

    If your system has been supplied with a Scroll pump then it must not be moved between systems without first consulting Genevac Service for advise. The Scroll pump will only function correctly on a Genevac Evaporator that has been upgraded to work with the Scroll pump.

  • Page 11: Safety

    Genevac HT-8 & HT-12 Series II Evaporating Systems Safety BEFORE OPERATING SYSTEM, IMPORTANT THAT THE FOLLOWING NOTES ARE READ TO ENSURE THAT THE IMPLICATIONS TO THE SAFETY OF PERSONNEL OPERATING THE SYSTEM AND FOR THE PROTECTION OF SAMPLE INTEGRITY ARE UNDERSTOOD.

  • Page 12: Safe Loading Of Rotor - General Document

    Use of Correct Accessories Genevac supply a wide range of sample holders to cover a variety of plate, tube, vial or flask formats. Where no holder is available, custom units can be made. In a few cases worldwide, users have developed their own holders and have collaborated with the Genevac R&D department to ensure the holders are suitable and...

  • Page 13: Adherence To Mass Limit

    Some users wish to run Genevac systems using the “Bohdan Miniblock” system as their sample holder. Please note, some (not all) of this range of items significantly exceed the mass limitations of a Genevac system. If you plan to use Bohdan Miniblocks in a Genevac system, please contact Genevac for a list of the relevant weights.

  • Page 14: Series I Vs Series Ii Swings

    It also has rounded and welded corners. Genevac offer a low cost upgrade to any user who has the old type of swing and who wishes to avoid the possibility of this misloading ever occurring Balancing of swings and sample holders.

  • Page 15: Good Procedural Practice

    Ensure only users familiar with all the issues outlined in this document are permitted to operate the equipment. • Only load swings and sample holders approved by Genevac. • System should be loaded and started by the same person. •...

  • Page 16: Safe Door Operation

    Genevac HT-8 & HT-12 Series II Evaporating Systems 4.3 Safe door operation The door is opened and closed manually but locked automatically by an electric actuator. Close and hold the door firmly against the door seal and press and hold the Door Close switch.

  • Page 17: How To Switch On The Pump

    Genevac HT-8 & HT-12 Series II Evaporating Systems 5.1 How to switch on the pump Connect the pump to the mains and switch on the mains. Switch the pump mains switch on. The pump will not be ready for use until the green ready light on the front panel is illuminated (Approx 7 minutes) –...

  • Page 18: Using The Keypad

    Genevac HT-8 & HT-12 Series II Evaporating Systems 5.3 Using the keypad The Series II keyboard controls and displays have been designed for ease and simplicity in use. The keyboard has the most used function keys on the left hand side.

  • Page 19: What The Screens Do

    Genevac HT-8 & HT-12 Series II Evaporating Systems 5.4 What the screens do There are three main control screens. The SELECT RUN screen lists the library of evaporation profiles you can choose. This screen has the capacity to store 100 different profiles, from which you can select a particular run or set of runs.

  • Page 20: Sample Holder Type

    Genevac HT-8 & HT-12 Series II Evaporating Systems Each line on the SELECT RUN screen is generated from the RUN DATA screen. When the chamber is vented and the rotor stationary, this screen is accessed by selecting VIEW/EDIT at the foot of the SELECT RUN screen and pressing START.

  • Page 21: Solvent

    Genevac HT-8 & HT-12 Series II Evaporating Systems Solvent When you select this field you are given a list of solvent types that have already been programmed into the evaporator. cursor keys move up and down. Press ENTER key to select (Highlighted in blue).
  • Page 22 Genevac HT-8 & HT-12 Series II Evaporating Systems With Auto Program Y[es] selected. The screen will now display Auto Programmed data Cyan (Light Blue). Save Run and continue If you did not select Auto Program or were not given the option,...

  • Page 23: Sampleguard Control Temperature

    Genevac HT-8 & HT-12 Series II Evaporating Systems SampleGuard Control Temperature Set within the range of - 20 to 70°C. SampleGuard Control Channel Channel 1 in Swing or Sample Holder. Channel 2 in Sample. Coolheat Enable Pressure Set within the range of 5–400 mbar.

  • Page 24: Rotor Speed

    Genevac HT-8 & HT-12 Series II Evaporating Systems Rotor Speed There are now 3 options available: High Rotor speed applies nominally 500 G. Rotor speed applies nominally 300 G. Very low Rotor speed = 500 rpm, applies nominally 50 G.

  • Page 25: During A Run

    Genevac HT-8 & HT-12 Series II Evaporating Systems During a run The main section of the RUN LOG screen, gives a graphical display of the real time changes in chamber pressure, spin rate, sample and sample holder temperatures. The top left hand section, displays the current...
  • Page 26 Genevac HT-8 & HT-12 Series II Evaporating Systems When the run is finished, after pressing a key, the following screen is displayed. Select the DRAIN or DEFROST OPERATIONS option using the numeric keys. Press ENTER display DRAIN DEFROST MENU. •...
  • Page 27 Genevac HT-8 & HT-12 Series II Evaporating Systems If you wish to end the work session on the system, go to the Select Run screen and press STOP. This prompt will displayed SELECT RUN screen. Press Y to close the system down or N to select another run.

  • Page 28: How To Enter Control Data

    Genevac HT-8 & HT-12 Series II Evaporating Systems • USER MENU displayed by pressing the MENU key only when the chamber is vented and the rotor stationary. • Lamp Layer Selection is not an option for the HT-4 or Mega.

  • Page 29: Run Data Locked

    Genevac HT-8 & HT-12 Series II Evaporating Systems When set to Yes this setting protects the run data Run Data Locked from inadvertent corruption. Press the ENTER key to toggle between Yes and Note that you will not be able to make amendments or additions if the data is locked.

  • Page 30: Sampleguard Control Temperature

    Genevac HT-8 & HT-12 Series II Evaporating Systems Select Done and press ENTER. Press ENTER and input the temperature you wish to SampleGuard Control Temperature limit the sample to during evaporation. NOT AVAILABLE FOR LYOPHILISATION This can be in the range of -20 to +70° C.

  • Page 31: Rotor Speed

    Genevac HT-8 & HT-12 Series II Evaporating Systems Press ENTER to display the Select Rotor Speed Rotor Speed drop down menu. Use the UP and DOWN cursor keys to select High, Low or Very Low (optional at order). High speed applies nominally 500 G and Low speed nominally 300 G.

  • Page 32: Heat Off Elapsed Time

    Genevac HT-8 & HT-12 Series II Evaporating Systems This can be in a range of 0 (lamps off for the entire Heat-Off Elapsed Time run) to 99 hours 59 minutes. This option is the elapsed time after which the run End-of-Run Option will be stopped.

  • Page 33: How To Use Sampleguard

    The use of Genevac Heat Transfer Plates would prove to be beneficial in preventing this condition arising, by providing an even heat transfer to your samples. Please refer to our “Options & Accessories Brochure” to get the best solution for needs.
  • Page 34 Genevac HT-8 & HT-12 Series II Evaporating Systems When positioning the sample probe, always ensure that the probe tip is located firmly at the bottom of the well, vial or tube. Note that the term TxD indicates that the transmitter is not transmitting and will be displayed when the rotor is not rotating.

  • Page 35: How To Use The Condenser

    Genevac HT-8 & HT-12 Series II Evaporating Systems How to use the condenser The VC3000D condenser fitted to your system has two condensing pots. The vacuum pot of 3 litre capacity, is connected in line between the evaporation chamber and the pump.
  • Page 36 Genevac HT-8 & HT-12 Series II Evaporating Systems AUTO DEFROST/DRAIN ENSURE THAT A SUITABLE RECEPTACLE IS CONNECTED TO THE DRAIN AT ALL TIMES, AS DRAINING OCCURS AUTOMATICALLY, EVEN IF THE RUN HAS COMPLETED OR NOT STARTED. ENSURE THAT THE END OF THE DRAIN HOSE IS ABOVE THE EXPECTED LEVEL OF WASTE SOLVENT IN THE CONTAINER.

  • Page 37: How To Start A Run

    Genevac HT-8 & HT-12 Series II Evaporating Systems How to start a run This section will describe a typical evaporation run of DMSO. In this example, two sets of eight 28 x 60 mm scintillation vials each containing 5 ml of DMSO were held in solid aluminium sample holders.
  • Page 38 Genevac HT-8 & HT-12 Series II Evaporating Systems Dispense the DMSO into the vials and load the sample holders into the sample swings. Always use the upper level when loading partial rotor loads. Always ensure that the vials, sample holders and sample swings are correctly and securely located.
  • Page 39 Genevac HT-8 & HT-12 Series II Evaporating Systems A brief discussion is given here to give the user a better understanding of the processes involved. When the run is started, the pressure will drop steadily as air is evacuated from the chamber and condenser.

  • Page 40: Getting The Best From Your System

    Genevac HT-8 & HT-12 Series II Evaporating Systems Getting the best from your system Routine Checks For high boiling point solvents such as DMSO, NMP, DMF and DMI the best evaporation rates will only be achieved at pressures better than 0.5 mbar.

  • Page 41: Problem Prevention

    Genevac HT-8 & HT-12 Series II Evaporating Systems Problem prevention Condensation of the evaporating solvent will occur Condensation when the solvent vapour temperature is above that of the chamber walls. This is most likely to occur with commonly used solvents such as NMP, DMI, DMSO and possibly DMF.

  • Page 42: Optimising A Run

    Genevac HT-8 & HT-12 Series II Evaporating Systems Use the Dri-Pure option on the drop down menu Limiting the chamber pressure under Pressure Control Regime on the Run Edit screen. • Bumping must be avoided, as it is a potential This option incrementally reduces the pressure and source of cross contamination between samples.

  • Page 43: Pre-Programmed Runs

    61 through to 98 are all associated with these pre-programmed methods, and methods 99 and 100 are for use by Genevac service engineers, please do not alter them. The pre-programmed methods have been designed to cover a group of solvents, such as high boiling point solvents.

  • Page 44: Care Of Your System

    (e.g. scratches) will not affect the performance of a holder or swing. Any structural damage, sufficient to bend of deform any part of the swing, holder or rotor - do not use - contact Genevac for evaluation. Cleaning: Loose dirt or debris can be removed using a brush and / or airline.

  • Page 45: How To Change The Pump Fluid (Cvp Only)

    Genevac HT-8 & HT-12 Series II Evaporating Systems How to change the pump fluid (CVP Only) Under some unusual conditions or for servicing purposes, it may be necessary to change or drain the pump fluid. Switch on the pump and run it for about 10 minutes.

  • Page 46: Maintenance And Service

    Because of the complexity of changing parts in the field, a high level of skill is required to maintain a complete system. To this end, Genevac can offer a range of preventative maintenance, service and breakdown contracts to cater for your particular needs.

  • Page 47: Additional Equipment

    There may be future applications for which your current range of sample holders are not adequate. The following accessories are just an example of the many ways in which Genevac can assist you in developing your system for the future.

  • Page 48: Moving Your System

    Genevac HT-8 & HT-12 Series II Evaporating Systems Moving your system These notes are included to assist you in moving your system to another area. Before moving your system, the following key points should be addressed. • Ensure that there is sufficient bench or fume cupboard space to position your system.
  • Page 49 Genevac HT-8 & HT-12 Series II Evaporating Systems If you have a Scroll Pump refer to the following: Connect the other end of the lead from the pump to this socket on the chamber Connect the control lead from the chamber to the condenser.
  • Page 50 Genevac HT-8 & HT-12 Series II Evaporating Systems If you have a CVP Pump refer to the following: Connect the other end of the lead from the pump to this socket on the chamber Connect the control lead from the chamber to the condenser.

  • Page 51: Fault Finding

    Genevac HT-8 & HT-12 Series II Evaporating Systems Fault Finding This section will describe potential errors and faults that may occur and lists their probable causes. The symptoms are listed on the left hand side of the page and the source of solutions on the right.
  • Page 52 Genevac HT-8 & HT-12 Series II Evaporating Systems General Probable cause • Suspected leak - pressure of no better than 5 • If the tube is satisfactory, reconnect the tube to mbar. the condenser and disconnect the heated inlet from the chamber at the condenser port.

  • Page 53: Technical Data

    Genevac HT-8 & HT-12 Series II Evaporating Systems Technical data HT-8 HT-12 Mechanical data Max rotor speed 1300 Low Speed 1300 Low Speed 1750 High Speed 1750 High Speed 1800 Dri-Pure 1800 Dri-Pure Max Force 300-500G 300-500G Drive system Direct...

  • Page 54: Operating

    Genevac HT-8 & HT-12 Series II Evaporating Systems Technical data (continued) Electrical Power supply 1 - 2.3kW 230V/50Hz, single phase 10A 208V/60 Hz, single phase 10A Power supply 2 – 1.5kW 230V/50Hz, single phase 6.5A 208V/60Hz, single phase 6.5A Environment...

  • Page 55: Ec Declaration Of Conformity

    Genevac HT-8 & HT-12 Series II Evaporating Systems 10 EC Declaration of Conformity 11 Safety We Genevac Limited Declare that this product: HT-8 Series II Evaporating System Complies with the relevant Essential Health and WARNING! Safety Requirements of the European Machinery...

  • Page 56: Warranty Statement

    This warranty is subject to the following provisions: 1. System must be sited, installed and operated in accordance with operator instruction manual. 2. Unit only used for purpose it was sold, and in accordance with Genevac published compatible solvent list.

  • Page 57: Annex A

    Getting the best from Auto Defrost Drain What is Auto-Defrost & Drain? Auto-Defrost and Drain is an option for the standard condensers on Genevac HT-4X, HT-8/12 series 2 and HT- 24 Workstation. Auto-Defrost and Drain enables the system to automatically drain the condenser of volatile solvent(s) between stages in a method, and at the end of the method to fully defrost and drain the system with no user intervention.
  • Page 58 • Drying of HPLC fractions where high vacuum in the final stages of evaporation is beneficial • Use of the Genevac fast lyophilisation method for HPLC fractions, where high vacuum in the latter stages of the process is essential •...
  • Page 59 Auto-Defrost and Drain at the end of the stage - this will be a full defrost and drain, as there are no more stages to follow. See appendix A for programming details. Please contact your local Genevac representative for further details on run times, alternatively please e-mail Applications@Genevac.com Page 58 of 04-4541 Issue 1-9 –...
  • Page 60 It is a very useful to have the Genevac Data Logging software running on an adjacent laptop or PC to collect the evaporation data which can then be sent to Genevac as an aid providing remote assistance.
  • Page 61 Genevac HT-8 & HT-12 Series II Evaporating Systems Appendix A Programming Guide for Drying HPLC Fractions Drying HPLC Fractions Run number Run Data Locked Next Run Number Run Name Fractions Fractions part 2 Fractions part 3 Sample Holder Type Your Holder...
  • Page 62 Genevac HT-8 & HT-12 Series II Evaporating Systems Appendix B Programming guide for fast lyophilisation of HPLC fractions Lyophilising HPLC Fractions Run number Run Data Locked Next Run Number Run Name Fractions Lyo Fractions Lyo part 2 Fractions Lyo part 3 Fractions Lyo part 4...
  • Page 63 Genevac HT-8 & HT-12 Series II Evaporating Systems Appendix C Programming guide for mixtures of high and low boiling point solvents Drying High - Low Mixtures Run number Run Data Locked Next Run Number Run Name High - Low Mix...
  • Page 64 Genevac HT-8 & HT-12 Series II Evaporating Systems NOTES 04-4541 Issue 1-9 – June 2008 Page 63 of Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 65: Useful Information

    If you need to contact Genevac for assistance, use The Sovereign Centre either the telephone or fax Hotlines given. Farthing Road It will always help Genevac Service if you have the Ipswich serial numbers at hand for the components of your IP1 5AP...
  • Page 66 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

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