Related Manuals for Genevac HT-24
Summary of Contents for Genevac HT-24
- Page 1 Genevac HT-24 Evaporation System User Manual Original instructions Part number 10-1562 Issue 3-7 March 2014...
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Page 3: Table Of Contents
Contents Introduction ........... 2 Loading Samples ........ 34 Sample holders ........34 Safety ............. 5 Use of correct accessories ....34 Warnings and cautions ......5 Mass limit ..........35 Risk assessment ........5 Sample holders and swings ....35 Precautions .......... -
Page 4: Introduction
They are simple to set up, easy to operate and very flexible. The HT-24 Workstation occupies the minimum amount of laboratory space, comprising two HT-8 or HT-12SII evaporators (or a combination of both) mounted on a workstation trolley with the option of an integrated fume hood. - Page 5 & electrical circuit breakers The optional fume hood fits on top of the HT-24 workstation and provides a totally enclosed system. There are three transparent sliding doors in the front for easy access to the evaporators and a window in each side. An exhaust spigot in the roof provides for connection to a suitable fume extraction system and a flow meter is located in the top right corner to monitor performance of the extraction system.
- Page 6 Genevac HT-24 User Manual Convenient pull-out sample shelves provide assistance when loading / unloading the evaporators. Fume hood flow meter Pull out sample shelves Automatic end of run detection software can be utilised to complete evaporation runs in the shortest possible time, opening the possibility for two or more runs to be completed in a day where previously overnight running may have been required.
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Page 7: Safety
Make sure exhaust / ventilation hoses are securely fitted and take solvent vapours away from the equipment and from the operator Only use sample holders and / or swings that are approved for use by Genevac Do not leave the system unevenly loaded, someone may start it ... -
Page 8: Combustible Solvents
Any maintenance or repair of this product, other that that which is detailed in this user manual, must be carried out by Genevac personnel (or approved representatives of Genevac) using only Genevac approved spare parts. -
Page 9: Options
Consult your Genevac representative for advice on any other requirements. Auto defrost and drain AD&D enables the uninterrupted evaporation of mixtures of solvents with dissimilar boiling points, including HPLC fractions. The system facilitates the use of the Genevac LyoSpeed™ fast lyophilisation process. High power lamps High power Coolheat IR lamps can significantly reduce evaporation times when evaporating solvents with high latent heat of evaporation (such as water, methanol or ethanol). -
Page 10: Sample Holders
The contact area is maximised to provide good physical support, optimum heat transfer and even heat distribution for drying loads. For further details of sample holders, contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac web site at www.genevac.com. -
Page 11: Basic Operation
User Manual Genevac HT-24 Basic Operation Power up / down Before switching the system on, make sure 3 x mains power leads are connected and the mains power is switched on. If the EMERGENCY STOP button has been pressed, push and twist to reset it, the emergency stop should not be used to power the system down on a regular basis. - Page 12 Genevac HT-24 User Manual The vacuum pump starts and the SELECT RUN menu appears on the evaporator LCD screen. The condenser also powers up and begins to cool down to its operating temperature. The system will not allow an evaporation run to commence until the condenser reaches 0°C;...
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Page 13: Opening / Closing The Door
User Manual Genevac HT-24 Opening / closing the door To open the door, press the OPEN CHAMBER # button on the workstation control panel. If necessary, wait for fumes to clear before opening the fume hood doors. To close and lock the door, hold the door in the closed position and press and hold the CLOSE button until a beep sounds to indicate the door is locked. -
Page 14: Workstation Controls
Genevac HT-24 User Manual Workstation controls Workstation control panel WASTE FULL: Lights-up when waste solvent container is full. OPEN CHAMBER 2: Press to open evaporator 2 (right-hand-side evaporator). ON indicator: Lights-up to indicate workstation is powered. EMERGENCY STOP: Press to isolate the workstation and stop both evaporators in an emergency. -
Page 15: Evaporator Controls
User Manual Genevac HT-24 Evaporator controls Frequently used function keys are positioned on the left hand side of the keyboard. The right side illuminates to accept input for programming via the Run data screen when required. LCD screen: Shows graphical and numerical data. -
Page 16: Lcd Screen
Genevac HT-24 User Manual LCD screen The illustration shows the LCD screen as it appears during an evaporation run, the graphical and numerical information shown relates to evaporation progress. Other menu screens appear during different operations and are discussed later in this User Manual. -
Page 17: Sampleguard
User Manual Genevac HT-24 SampleGuard™ The SampleGuard transmitter has two input connectors for SampleGuard probes, these must be connected for the SampleGurard system to function correctly. The numbers 1 and 2, embossed on the side of the SampleGuard transmitter, correspond to the SampleGuard channels shown on the LCD screen. -
Page 18: Selecting A Run
The use of Genevac Heat Transfer Plates reduces the effect by providing an even heat transfer across the plate. For details of the range of heat transfer plates that are available, contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac website at www.genevac.com. -
Page 19: Run In Progress
User Manual Genevac HT-24 Run in progress The Run Log screen provides data relating to evaporation progress, information is presented in numerical and graphical form. During evaporation, the effect of evaporative cooling (on the sample being monitored) can be seen in the divergence of the SampleGuard channel 1 and channel 2 traces. When the traces begin to converge, this indicates the sample is approaching dryness. -
Page 20: End Of Run
Genevac HT-24 User Manual End of run This screen appears after the run finishes. Select Skip, Drain or Defrost using the numeric keys indicated. Skip: The evaporator returns to the Run Select screen. Drain: The system opens the drain valve and pumps any waste solvent into the waste solvent container. -
Page 21: Advanced Operation
User Manual Genevac HT-24 Advanced Operation The evaporator holds a library of up to 100 individual methods (or runs) which can be accessed using the SELECT RUN screen. Runs 50 to 99 are pre-set at the factory and are generic; they are designed to get the system up and running quickly. To optimise the system for specific applications, new methods can be created. -
Page 22: Run Parameters
Genevac HT-24 User Manual Run parameters The following pages describe the function of each run parameter in turn and show the range of setting options available. Instructions for setting the parameter are also given. Run Data Protection The run profile can be protected against accidental alteration. Set the Run Data Protection field to No before attempting to edit an existing run profile or set to Yes to prevent accidental changes being made to the run profile. - Page 23 User Manual Genevac HT-24 Solvent Commonly used solvents can be selected from the window that opens (more than one solvent may be selected if using a mixture). Once selected, solvents are highlighted in blue. Select Done when the solvent or solvents have been selected. For solvents that are not listed, select Other and enter the solvent name manually (no more than 31 characters).
- Page 24 Genevac HT-24 User Manual Sample Volume Enter a value for the volume of sample in each individual well / tube / vial. Note: Once selections for Sample Holder Type, Sample Volume and Solvent have been made from the drop down lists, an option to Auto Program is offered.
- Page 25 User Manual Genevac HT-24 SampleGuard Control Temperature Enter a temperature value up to +70°C. The system heats the sample holders to this temperature. Caution: Risk to Sample Integrity; consider the possible effects on the dried sample when selecting a safe control temperature.
- Page 26 Note: When waiting to cool, the chamber temperature will not drop below the ambient temperature. If in doubt contact Genevac Applications Support for advice. 10-1562 Issue 3-7 March 2014...
- Page 27 User Manual Genevac HT-24 Rotor Speed Select from the options: High Increased centrifugal force (approximately 500 G) helps to hold the samples in place. Select High If using microtitre plates, very full tubes, solvents or mixtures that are prone to bumping, or if at all unsure of the any of the above.
- Page 28 Genevac HT-24 User Manual Heat-Off Option Select from the options: Elapsed Time Manually enter a time for the Heat-Off Option, the evaporator turns off the IR lamps when the time elapses and continues to run without heat input until the end-of-run time elapses.
- Page 29 User Manual Genevac HT-24 Pressure Control Regime Select from the options: Full Vacuum The evaporator reduces the pressure in the chamber to the lowest attainable level. Controlled Pressure Enter the Controlled Pressure required, the evaporator reduces the pressure in the chamber to this preset level.
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Page 30: Run Log
Genevac HT-24 User Manual Run log The Run Log screen allows sample drying progress to be monitored during the evaporation process; information is presented numerically with relevant data also presented graphically to reveal change trends in real time. Numerical values are shown for: ... -
Page 31: Controls Menu
COPY TO: copies the highlighted run profile may to a different location DRAIN: drains the condenser DEFROST: enters the Condenser Defrost menu FLUSH: not applicable on HT-24 OPTIONS: enters the Options Menu. 10-1562 Issue 3-7 March 2014... -
Page 32: Data Transfer
COPY LOG FILES TO DATAKEY: Log files for the last four evaporation runs can be downloaded for method validation, troubleshooting or method development purposes SAVE SYSTEM INFO TO DATAKEY: For troubleshooting use by Genevac Service department COPY SETUP.INI FROM DATAKEY: For use by Genevac Service Engineers. -
Page 33: Condenser
Intermediate (short) defrost and drain is permitted but full defrost and drain should not be used. Instead, stop the system, open the door and then perform a manual defrost and drain. For this application, Genevac strongly recommend the use a fume cupboard or fume extraction system. -
Page 34: Manual Defrost And Drain
To empty, open the waste solvent container door, lift the waste solvent container from its tray and place it on the floor in front of the HT-24. The waste solvent drain pipe will have either a quick release connector or a quarter turn valve. - Page 35 User Manual Genevac HT-24 Systems with a quick release connector Disconnect the waste solvent hose and breather hose from the waste solvent container. Remove the cap and static dissipating rod from the waste solvent container as shown. Systems with a quarter turn valve Close the valve, loosen the red cap on top of the waste solvent container (approximately one turn, counter-clockwise) to release the drain pipe then pull the drain pipe out of the container.
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Page 36: Loading Samples
Genevac HT-24 User Manual Loading Samples The following instructions relate to Genevac systems with swung rotors. Your local Genevac representative will be happy to provide information about systems with fixed rotors which have angled holes for holders or individual tubes. -
Page 37: Mass Limit
Genevac. Mass limit The mass limit for Genevac HT-SII evaporators is 1.5 kg per swing. This is the total mass that can be loaded onto each position of a swung rotor, and includes: ... -
Page 38: Loading The Rotor
Rotate the rotor by hand. Check that all tubes, holders, plates, etc are correctly located and swings are able to swing freely. Only use the sample holders that were supplied with the system or are specified for use with the system; never use non Genevac approved sample holders. -
Page 39: Balancing The Rotor
User Manual Genevac HT-24 Balancing the rotor Always make sure that swings of the same static weight are placed opposite each other on the rotor. Caution: Opposing swings must be identical (the same type and version) ideally the same version of swing should be used in all four positions of each rotor level. -
Page 40: Method Guide
Note: Runs 99 and 100 are reserved for use by Genevac service engineers. Each of the listed runs is designed to work for a group of solvents. Please bear in mind it is usually possible to further optimise methods for specific solvents. -
Page 41: Creating New Methods
When performing lyophilisation, fill all positions of the sample holders as samples in partially filled holders may not freeze. It normally requires several trials to establish a robust lyophilisation method, your local Genevac representative will be happy to assist with this. 10-1562 Issue 3-7 March 2014... -
Page 42: Acceptable Solvents
Conduct a risk assessment for each solvent and solvent mixture before use, the presence of solvents on this list indicates only that they will not damage the system in normal use. If in doubt, please contact your Genevac sales representative for further information. 10-1562 Issue 3-7 March 2014... -
Page 43: Solvent Mixtures
User Manual Genevac HT-24 Solvent mixtures When creating methods for solvent mixtures, linked runs can be used to optimise the evaporation process; volatile components can be evaporated and then removed from the condenser before a high vacuum stage completes the non volatile part of the mixture. -
Page 44: Method Examples
Genevac HT-24 User Manual Method examples The following examples show suggested settings for some common applications. Example 1. Drying HPLC Fractions Method consists of three runs linked together to form a multi stage run, with settings as follows: Stage 1... - Page 45 User Manual Genevac HT-24 Example 2. Fast Lyophilisation of HPLC Fractions Method consists of three runs linked together to form a multi stage run, with settings as follows: Stage 1 Stage 2 Stage 3 Ramp & Remove Freeze & Primary...
- Page 46 Genevac HT-24 User Manual Example 3. Mixture of High and Low Boiling Point Solvents Method consists of three runs linked together to form a multi stage run, with settings as follows: Stage 1 Stage 2 Sage 3 Remove Organic Dry High BP...
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Page 47: Maintenance
In order to monitor system performance, Genevac recommend keeping a log of the time taken for the system to reach full vacuum and the ultimate vacuum achieved. -
Page 48: General Cleaning
Genevac HT-24 User Manual General cleaning Cleaning should be performed as and when required. Depending upon the solvents being evaporated, this may range from monthly to after every use. Wipe down the exterior surfaces of the system with a dampened cloth or paper towel; do not apply cleaning solvents to the condenser pot lid. -
Page 49: Planned Maintenance And Servicing
In order to maintain peak performance and avoid costly and unscheduled down time, Genevac strongly recommend implementing a schedule of planned maintenance. Changing parts in the field is complex and requires a high level of skill. Genevac offers a range of preventive maintenance, service and breakdown contracts designed to keep the equipment in top condition. -
Page 50: Delivery And Installation
Genevac HT-24 User Manual Delivery and Installation To ensure that your new Genevac HT system is set up correctly and operates to its full potential, it should be installed and commissioned by Genevac personnel or by an authorised representative of Genevac. -
Page 51: Troubleshooting
User Manual Genevac HT-24 Troubleshooting Audible alerts Notification Button acknowledge Short beep Door lock acknowledge Short beep Illegal button press Short double beep Evaporator stopped Three short beeps when the system comes to rest. Repeats every minute until the door is opened Critical error Three short beeps when the system comes to rest. -
Page 52: Condensation
Carbonised deposit from samples IR lamp glass cracked A range of specialised sample holders is available to protect the quartz lenses. Contact Genevac Sales for details. 10-1562 Issue 3-7 March 2014... -
Page 53: Bumping
Resettable mains power circuit breakers are located above the vacuum pump. If a circuit breaker trips, it may be the result of an electrical failure. If a breaker trips repeatedly, contact Genevac service for assistance. Mains power circuit breakers 10-1562 Issue 3-7 March 2014... -
Page 54: Rectifying Faults And Errors
User Manual Rectifying faults and errors If one of the following problems occurs, it can normally be addressed by the user; if a fault occurs that is not listed, contact your local Genevac representative for advice or technical support. Symptom... - Page 55 See IR lamp / fan failure above SampleGuard fault See SampleGuard error above Poor vacuum Check vacuum connectors. Contact Genevac service if leak cannot be found Aqueous samples freezing Set controlled pressure to 8 mbar for due to evaporative cooling...
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Page 56: Technical Data
Genevac HT-24 User Manual Technical Data Specifications Evaporator Storage / transportation environment Ambient temperature 0°C to 40°C Max rotor speed 1745 rpm Relative humidity 10 o 80% non- condensing Max load per swing 1.5 kg Altitude Sea level to 12000 m... -
Page 57: Ec Declaration Of Conformity
* Note that under the Machinery Directive, the limit for earth leakage current for Class1 portable equipment is specified at 3.5 mA. Whilst every effort has been taken to minimise the leakage current of the HT-24, it may slightly exceed this value at, typically, 4.6 mA. -
Page 58: Warranty Statement
Exhaust vapours must be ducted away from the system, ideally to a suitable laboratory fume extraction system. The system is only used for purpose it was sold, and in accordance with Genevac published compatible solvent list. Preventative maintenance and cleaning must be carried out as detailed in the Maintenance section of this User manual. -
Page 59: Amendment Control
User Manual Genevac HT-24 Amendment control Issue Reason for change Date issued Opening door without power instructions added. Format and layout 20-Dec-11 revised Cover page revised. Specifications updated. 28-Mar-12 Flusher reference removed. Condenser drain pump / filter added to Troubleshooting. (10-1562A restricted distribution for systems... -
Page 60: Useful Information
Genevac Limited. applications@genevac.com If you need to contact Genevac for assistance, use either the telephone or fax Web site: Hotlines shown. Please have the instrument serial number at hand.
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