Related Manuals for Genevac HT-4X
Summary of Contents for Genevac HT-4X
- Page 1 Genevac HT-4X Evaporation System with XDS5 vacuum pump User Manual Original instructions Part number 04-7415 Issue 1-16 August 2014...
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Page 3: Table Of Contents
Contents Introduction ..........2 Loading Samples ........32 Sample holders ..........32 Safety ............4 Use of correct accessories ......32 Warnings and cautions ........4 Mass limit ............. 33 Risk assessment ..........4 Sample holders and swings ......33 Precautions ............. -
Page 4: Introduction
Introduction Genevac HT evaporation systems are designed to provide very high performance coupled with ease of use. They are simple to set up, easy to operate and very flexible. HT-4X comprises a built-in cryopump-condenser and a separate vacuum pump. Programmable software can store up to 100 methods (or runs) which can be configured independently or can be linked together to form multi stage programmes for a wide variety of solvent removal applications. - Page 5 User Manual Genevac HT-4X Evaporation System Automatic end of run detection software can be utilised to complete evaporation runs in the shortest possible time, opening the possibility for two or more runs to be completed in a day where previously overnight running may have been required.
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Page 6: Safety
Make sure exhaust / ventilation hoses are securely fitted and take solvent vapours away from the equipment and from the operator Only use sample holders and / or swings that are approved for use by Genevac Do not leave the system unevenly loaded, someone may start it ... -
Page 7: Combustible Solvents
Emergency shutdown All Genevac evaporators are designed to be safe with respect to samples when they are switched off in an emergency. The system will automatically vent and the rotor will spin down slowly when power is removed. To shutdown in an emergency, switch off the power socket at the wall and unplug (or simply unplug if there is no switch). -
Page 8: Options
Genevac HT-4X Evaporation System User Manual Options Standard equipment HCl resistance Genevac HT-4X evaporators feature the All vulnerable parts of the evaporator are following equipment as standard: manufactured from inert material. A special software feature conditions the vacuum ® ... -
Page 9: Infinity Trolley
For further details of sample holders, contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac web site at www.genevac.com. eXalt crystallisation package The package consists of additional software features plus a tool kit that provides an array of baffles. -
Page 10: Basic Operation
Genevac HT-4X Evaporation System User Manual Basic Operation Power up / down Before switching on, make sure the pump control cable is connected as shown, the power cable is connected to a mains power outlet and the power is switched on. - Page 11 User Manual Genevac HT-4X Evaporation System To start the system, press the green START button on the evaporator keypad. The vacuum pump starts and the SELECT RUN menu appears on the evaporator LCD screen. The condenser also powers up and begins to cool down to its operating temperature. The system will not allow an evaporation run to commence until the condenser reaches 0°C;...
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Page 12: Opening / Closing The Lid
Genevac HT-4X Evaporation System User Manual Opening / closing the lid To open and close the lid, press the button on the front panel. Note: The lid cannot be opened while the rotor is turning or the evaporation chamber is below atmospheric pressure. -
Page 13: Controls
User Manual Genevac HT-4X Evaporation System Controls Frequently used function keys are positioned on the left hand side of the keyboard. The right side illuminates to accept input for programming via the Run data screen when required. LCD screen: Shows graphical and numerical data. -
Page 14: Lcd Screen
Genevac HT-4X Evaporation System User Manual LCD screen The illustration shows the LCD screen as it appears during an evaporation run, the graphical and numerical information shown relates to evaporation progress. Other menu screens appear during different operations and are discussed later in this User Manual. -
Page 15: Sampleguard
User Manual Genevac HT-4X Evaporation System SampleGuard™ The SampleGuard transmitter has two input connectors for SampleGuard probes, these must be connected for the SampleGurard system to function correctly. The numbers 1 and 2, embossed on the side of the SampleGuard transmitter, correspond to the SampleGuard channels shown on the LCD screen. -
Page 16: Selecting A Run
The use of Genevac Heat Transfer Plates reduces the effect by providing an even heat transfer across the plate. For details of the range of heat transfer plates that are available, contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac website at www.genevac.com. -
Page 17: Run In Progress
User Manual Genevac HT-4X Evaporation System Run in progress The Run Log screen provides data relating to evaporation progress, information is presented in numerical and graphical form. During evaporation, the effect of evaporative cooling (on the sample being monitored) can be seen in the divergence of the SampleGuard channel 1 and channel 2 traces. -
Page 18: End Of Run
Genevac HT-4X Evaporation System User Manual End of run This screen appears after the run finishes. Select Skip, Drain or Defrost using the numeric keys indicated. Skip: The evaporator returns to the Run Select screen. Drain: The system opens the drain valve and pumps any waste solvent into the waste solvent container. -
Page 19: Advanced Operation
User Manual Genevac HT-4X Evaporation System Advanced Operation The evaporator holds a library of up to 100 individual methods (or runs) which can be accessed using the SELECT RUN screen. Runs 50 to 99 are pre-set at the factory and are generic;... -
Page 20: Run Parameters
Genevac HT-4X Evaporation System User Manual Run parameters The following pages describe the function of each run parameter in turn and show the range of setting options available. Instructions for setting the parameter are also given. Run Data Protection The run profile can be protected against accidental alteration. Set the Run Data Protection field to No before attempting to edit an existing run profile or set to Yes to prevent accidental changes being made to the run profile. - Page 21 User Manual Genevac HT-4X Evaporation System Solvent Commonly used solvents can be selected from the window that opens (more than one solvent may be selected if using a mixture). Once selected, solvents are highlighted in blue. Select Done when the solvent or solvents have been selected. For solvents that are not listed, select Other and enter the solvent name manually (no more than 31 characters).
- Page 22 Genevac HT-4X Evaporation System User Manual Sample Volume Enter a value for the volume of sample in each individual well / tube / vial. Note: Once selections for Sample Holder Type, Sample Volume and Solvent have been made from the drop down lists, an option to Auto Program is offered.
- Page 23 User Manual Genevac HT-4X Evaporation System SampleGuard Control Temperature Enter a temperature value up to +70°C. The system heats the sample holders to this temperature. Caution: Risk to Sample Integrity; consider the possible effects on the dried sample when selecting a safe control temperature.
- Page 24 Note: When waiting to cool, the chamber temperature will not drop below the ambient temperature. If in doubt contact Genevac Applications Support for advice. 04-7415 Issue 1-16 August 2014...
- Page 25 User Manual Genevac HT-4X Evaporation System Rotor Speed Select from the options: High Increased centrifugal force (approximately 500 G) helps to hold the samples in place. Select High If using microtitre plates, very full tubes, solvents or mixtures that are prone to bumping, or if at all unsure of the any of the above.
- Page 26 Genevac HT-4X Evaporation System User Manual Heat-Off Option Select from the options: Elapsed Time Manually enter a time for the Heat-Off Option, the evaporator turns off the IR lamps when the time elapses and continues to run without heat input until the end-of-run time elapses.
- Page 27 User Manual Genevac HT-4X Evaporation System Pressure Control Regime Select from the options: Full Vacuum The evaporator reduces the pressure in the chamber to the lowest attainable level. Controlled Pressure Enter the Controlled Pressure required. The evaporator reduces the pressure in the chamber to this preset level.
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Page 28: Run Log
Genevac HT-4X Evaporation System User Manual Run log The Run Log screen allows sample drying progress to be monitored during the evaporation process; information is presented numerically with relevant data also presented graphically to reveal change trends in real time. -
Page 29: Options Menu
User Manual Genevac HT-4X Evaporation System Options menu Select OPTIONS from the horizontal menu bar, the following options appear: Lamp Layer Selection: disable lamp layers if second or third rotor levels are not used. Operator Controls: opens the Controls Menu: Keypress Beep On / Off: turns off the key-press beep response. -
Page 30: Menu Bar
Genevac HT-4X Evaporation System User Manual Menu bar The default position for the horizontal menu bar cursor is with START highlighted. Press the cursor direction left and right keys to return the cursor to START after using any of the other menu options. -
Page 31: Data Transfer
The evaporator requires a sub-directory COPY SETUP.INI FROM DATAKEY: entitled: Series2 to be present on the data For use by Genevac Service Engineers. key. Confirmation requests or advice messages When the evaporator transfers files to a may appear on the LCD screen and file data key for the first time, it creates a transfer progress is indicated. -
Page 32: Condenser
Genevac HT-4X Evaporation System User Manual Condenser The condenser is controlled by the Automatic defrost and drain also helps evaporator so requires little intervention achieve excellent final dryness when from the user. Using the programmable evaporating HPLC fractions. features of the evaporator’s software,... -
Page 33: Manual Defrost And Drain
User Manual Genevac HT-4X Evaporation System Manual defrost and drain Waste solvent container When automatic defrost and drain cannot Check the condition of the waste solvent be used, the condenser should be defrosted container and waste solvent drain hose and drained manually. The term “manual regularly. -
Page 34: Loading Samples
For use in side-bridge swing only. Note: Some Bohdan Miniblock system sample holders exceed the mass limitations of Genevac systems. If you plan to use Bohdan Miniblocks in a Genevac system, Tube holder and side bridge swing please contact Genevac for a list of the relevant weights. -
Page 35: Mass Limit
User Manual Genevac HT-4X Evaporation System Mass limit The mass limit for Genevac HT-SII evaporators is 1.5 kg per swing. This is the total mass that can be loaded onto each position of a swung rotor, and includes: The swing ... -
Page 36: Loading The Rotor
Rotate the rotor by hand. Check that all tubes, holders, plates, etc are correctly located and swings are able to swing freely. Only use the sample holders that were supplied with the system or are specified for use with the system; never use non Genevac approved sample holders. -
Page 37: Balancing The Rotor
User Manual Genevac HT-4X Evaporation System Balancing the rotor Always make sure that swings of the same static weight are placed opposite each other on the rotor. Caution: Opposing swings must be identical (the same type and version) ideally the same version of swing should be used in all four positions of each rotor level. -
Page 38: Method Guide
Note: Runs 99 and 100 are reserved for use by Genevac service engineers. Each of the pre-programmed runs is designed to work for a group of solvents. Please bear in mind it is usually possible to further optimise methods for specific solvents. -
Page 39: Creating New Methods
Run options, may employ single automatic programming software (refer to SampleGuard channel temperature control. Advanced Operation – Setting-up a Genevac are able to provide remote method). Alternatively, existing methods assistance by analysing evaporation data can copied to a new location using the from a log file. -
Page 40: Crystallisation
Genevac HT-4X Evaporation System User Manual Crystallisation For evaporators equipped with the optional eXalt crystallisation package, the Crystallise option is accessed from Pressure Control Regime in the Run Data screen. Selecting Crystallise allows the user to enter control parameters for pressure reduction cycles. -
Page 41: Acceptable Solvents
Carry out a risk assessment for each solvent or solvent mixture before use. Solvents included in the following table are not damaging to the evaporator providing appropriate methods are used. Contact your local Genevac representative for advice before using solvents which are not. -
Page 42: Solvent Mixtures
Genevac HT-4X Evaporation System User Manual Solvent mixtures When creating methods for solvent mixtures, linked runs can be used to optimise the evaporation process; volatile components can be evaporated and then removed from the condenser before a high vacuum stage completes the non-volatile part of the mixture. -
Page 43: Method Examples
User Manual Genevac HT-4X Evaporation System Method examples The following examples show suggested settings for some common applications. Example 1. Drying HPLC Fractions Method consists of three runs linked together to form a multi stage run, with settings as follows:... - Page 44 Genevac HT-4X Evaporation System User Manual Example 2. Fast Lyophilisation of HPLC Fractions Method consists of three runs linked together to form a multi stage run, with settings as follows: Stage 1 Stage 2 Stage 3 Stage 4 Ramp & remove...
- Page 45 User Manual Genevac HT-4X Evaporation System Example 3. Mixture of High and Low Boiling Point Solvents Method consists of three runs linked together to form a multi stage run, with settings as follows: Stage 1 Stage 2 Sage 3 Remove Organic...
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Page 46: Maintenance
Any maintenance or repair of this product, Periodic maintenance other than that specified within this user manual, must be carried out by Genevac Check the condition of the exhaust hose and personnel (or approved representatives of waste solvent drain tubes: Genevac) using only approved spare parts. -
Page 47: General Cleaning
User Manual Genevac HT-4X Evaporation System Clean off any adhered dirt or debris by washing the sample holders in soapy water General cleaning or using a cleaning solvent such as methanol or acetone. The sample holders Cleaning should be performed as and when should then be rinsed in clean water. -
Page 48: Ir Lamp Lens
Changing parts in the field is complex and For systems fitted with an optional requires a high level of skill. Genevac offers condenser flusher, fill the flusher jar with a range of preventive maintenance, service... -
Page 49: Delivery And Installation
User Manual Genevac HT-4X Evaporation System Delivery and Installation Check the contents of the delivery, against Consult Genevac for advice on any other the delivery note as soon as possible. positioning requirements. Notify your local Genevac representative immediately if any parts are missing or Make sure the exhaust hose is free from damaged. -
Page 50: Troubleshooting
Genevac HT-4X Evaporation System User Manual Troubleshooting Audible alerts Notification Button acknowledge Short beep Door lock acknowledge Short beep Illegal button press Short double beep Evaporator stopped Three short beeps when the system comes to rest. Repeats every minute until the door is opened Critical error Three short beeps when the system comes to rest. -
Page 51: Condensation
User Manual Genevac HT-4X Evaporation System Condensation TFA creep Solvent condensation within the evaporator TFA creep is the movement of TFA up the is most likely to occur with high boiling point inside of the tubes, vials or microtitre plates. -
Page 52: Bumping
Genevac HT-4X Evaporation System User Manual Bumping Opening the lid without power Bumping is unpredictable and can occur In the event of power failure, the vacuum with any solvent mixture; it can cause cross system vents automatically, the rotor may contamination between samples and can take up to two minutes to stop spinning. -
Page 53: Circuit Breakers / Fuses
Genevac HT-4X Evaporation System Circuit breakers / fuses If a fuse blows, it may be the result of an electrical failure; fuses should only be replaced by suitably trained personnel. If a fuse blows repeatedly, contact Genevac service for assistance. Evaporator The evaporator fuse is located on the lower right-hand side of the evaporator back panel. -
Page 54: Other Troubleshooting
Genevac HT-4X Evaporation System User Manual Other troubleshooting If one of the following problems occurs, it can normally be addressed by the user; if a fault occurs that is not listed, contact your local Genevac representative for advice or technical support. Symptom Cause... -
Page 55: Technical Data
User Manual Genevac HT-4X Evaporation System Technical Data Specifications Evaporator Emissions Max rotor speed 1745 rpm Typical noise level is 69 dB (A) at one metre Max load per swing 1.5 kg from the evaporator during normal operation. Max operational imbalance... -
Page 56: Ec Declaration Of Conformity
In non-laboratory environments this product might cause interference, in which case the user is required to take appropriate actions. Signed: Name: G Broadbent Position: Research and Development Manager Date: July 2014 Being the person appointed by Genevac Ltd to sign on their behalf 04-7415 Issue 1-16 August 2014... -
Page 57: Warranty Statement
Exhaust vapours must be ducted away from the system, ideally to a suitable laboratory fume extraction system. The system is only used for purpose it was sold, and in accordance with Genevac published compatible solvent list. Preventative maintenance and cleaning must be carried out as detailed in the Maintenance section of this User manual. -
Page 58: Amendment Control
Genevac HT-4X Evaporation System User Manual Amendment control Issue Reason for change Date issued First issue, based on 10-1450 with layout changes and references to early 20-Dec-11 build variants removed. 1-2 Cover page Revised. Specifications updated. 29-Mar-12 Flusher option instructions added. Pre-programmed runs table replaced with screen shot. -
Page 60: Useful Information
Genevac Limited. applications@genevac.com If you need to contact Genevac for assistance, use either the telephone or fax Web site: Hotlines shown. Please have the instrument serial number at hand.
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