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Evident BX3-UCD8A Instructions Manual

Motorized universal condenser
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INSTRUCTIONS
BX3-UCD8A
MOTORIZED UNIVERSAL CONDENSER
Optical Microscope Accessory
This manual pertains only to the information related to the universal condenser. Before using this
instrument together with the microscope and associated modules, make sure that you have carefully
read their manuals and understand how the system should be operated together.

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Summary of Contents for Evident BX3-UCD8A

  • Page 1 INSTRUCTIONS BX3-UCD8A MOTORIZED UNIVERSAL CONDENSER Optical Microscope Accessory This manual pertains only to the information related to the universal condenser. Before using this instrument together with the microscope and associated modules, make sure that you have carefully read their manuals and understand how the system should be operated together.
  • Page 2 This product is a part of the system product which complies with the CE marking. Please refer to the instruction manual of your system product for the safety instructions related to the CE marking. In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately.

  • Page 3: Table Of Contents

    BX3-UCD8A CONTENTS IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1 SYSTEM CHART 2 NOMENCLATURE 3 OBSERVATION PROCEDURES 6-19 3-1 Preparation .................................................... 6 3-2 Brightfield Observation (BF) ........................................6-8 3-3 Phase Contrast Observation (PH) ....................................9, 1 0 3-4 Differential Interference Contrast Observation (DIC) ........................

  • Page 4: Getting Ready

    BX3-CBH control box, the touch panel controller. · When the BX3-UCD8A is installed on the BX53/43 manual microscope frame to which the U-CBM control box is connected, the semi-motorized operations of the condenser can be controlled from the U-HSCBM hand switch.

  • Page 5: Maintenance And Storage

    BX3-UCD8A 7. Remove the condenser from the microscope before mounting or dismounting optical elements. 8. Do not tighten the optical element centering knobs too much. 9. Do not tighten the optical element centering knobs while the optical element is not present. Otherwise, the knobs may become unable to be returned.

  • Page 6: System Chart

    BX3-CBH Microscope frame BX63* Touch panel controller Motorized Universal Microscope frame Condenser BX53/BX43 BX3-UCD8A* Interface cable U-IFCBL200 Control box U-CBM *This condenser cannot be used if the IX-SVL2 Hand switch for CBM cross stage is installed on the BX63. U-HSCBM...

  • Page 7: Nomenclature

    * To improve the ease of operation, replace the centering knobs and clamping knob of the condenser holder and the clamping knob of the stage with these screws. Mount BX3-UCD8A with one condenser centering screw (right) @ on the right side facing to the condenser and the other condenser centering screw (left) 2 on the left side.
  • Page 8 Optical elements (See pages 26 to 28 for the list of applicable objectives.) DIC prism (Large) Phase contrast ring (Small) DIC prism (Small) U-PH1S U-DP10S DIC prisms other than U-PH2S U-DP10S U-PH3S Low-magnification conversion Darkfield ring Phase contrast ring (Large) lens for UCD U-DFA U-PH3...

  • Page 9: Observation Procedures

    BX3-UCD8A OBSERVATION PROCEDURES 3-1 Preparation · If the top lens and optical element are not assembled, go to Chapter 6, “ASSEMBLY” (pages 24 to 35). · Set the main switch of the BX3-CBH (or U-CBM) control box to “ I ” (ON) and make the microscope system ready for observation.
  • Page 10 } If a transmitted DIC slider (U-DICT, U-DICTS, etc.) is used, pull out the slider till the click position for disengaging it from the light path. } If the reflected light analyzer (U-AN-2) is used, pull out the analyzer till the click position for disengaging it from the light path.
  • Page 11 BX3-UCD8A Field Iris Diaphragm Aperture iris 70-80% { The field iris diaphragm restricts the diameter of the beam of light entering diaphragm image the objective and thus excludes extraneous light, improving image contrast. 30-20% The diameter of the field iris should e adjusted for objective power to the extent that it just circumscribes the field of view.

  • Page 12: Phase Contrast Observation

    3-3 Phase Contrast Observation (PH) Applicable objective magnifications Magnification 100X Top lens IN * Slight flare may be observed in the peripheral area during super-widefield (FN 26.5) observation, but this does not cause problem in image recording. } If a transmitted DIC slider (U-DICT, U-DICTS, etc.) is used, pull out the slider till the click position for disengaging it from the light path.
  • Page 13 BX3-UCD8A 6. Rotate the top of the U-CT30 centering telescope to focus on the bright ring (ring slit) and dark ring (phase film of the objective) in the field of view. 7. Push in and rotate the optical element centering knobs to adjust centering of the phase contrast ring so that the bright ring in the field overlaps with the dark ring (Fig.

  • Page 14: Differential Interference Contrast Observation (Dic)

    3-4 Differential Interference Contrast Observation (DIC) Applicable objective magnifications Magnification 100X Top lens IN A transmitted DIC slider and an analyzer (U-ANT or U-AN-2) are required for the DIC observation. A polarizer is also required; engage the one built into the condenser in the light path. When the U-DICT slider is used Clamping knob Revolving nosepiece in which DIC...
  • Page 15 BX3-UCD8A When the U-DICTS/U-DICTHC/U-DICTHR slider is used Clamping knob** Analyzer (U-AN-2) Prism position selector lever Pushed in: BFP1 (To be mounted in the mount Pulled out: NORMAL of the vertical illuminator) Revolving nosepiece in which DIC can be mounted (U-D7REA, U-D7RES, etc.)
  • Page 16 1. Adjust the polarizer in the following steps. a) Engage the transmitted light DIC slider in the light path and tighten the clamping knob. b) Engage the U-ANT or U-AN-2 analyzer in the light path. 2. Select the BF observation light path (with no optical element engaged in the light path). 3.
  • Page 17 BX3-UCD8A When the U-DICT and U-AN-2 are used a) Pull out the transmitted light DIC slider to disengage it from the light path. b) Look into an eyepiece, loosen the polarizer clamping knob and rotate the polarizer rotation knob until the field of view is completely dark.
  • Page 18 9. Adjust the background color contrast using the prism moving knob of the transmitted light DIC slider as described below. a) Rotate the prism moving knob to select the interference color that can provide the specimen image with the best contrast.

  • Page 19: Darkfield Observation

    BX3-UCD8A 3-5 Darkfield Observation (DF) Applicable objective magnifications Magnification 100X Top lens IN *The peripheral area of the field may be obscured during super-widefield (FN 26.5) observation, but this does not cause problem in camera recording. **Only the objectives with NA of 0.7 or less can be used. (An iris-equipped objective capable of stopping down NA to 0.7 or less can also be used.)

  • Page 20: Simplified Polarized Light Observation (Kpo)

    7. Move the condenser up-down to the position where uniform darkfield illumination can be obtained. 8. Open the field iris diaphragm as far as the brightness does not become irregular. Do not look into the eyepieces when switching the objective during darkfield observation or when switching CAUTION the observation mode from darkfield observation to another.
  • Page 21 BX3-UCD8A } An analyzer (U-ANT or U-AN-2) is required for the simplified polarized light observation. If the vertical fluorescence illuminator is not installed, install the transmitted analyzer (U-ANT) by referring to its instruction manual. } If the vertical fluorescence illuminator is installed, Attach the reflected light analyzer (U-AN-2) by referring to the instruction manual for the vertical fluorescence illuminator.
  • Page 22 General operating precautions · If the U-excited reflected fluorescence observation is performed with the 10X or 20X objective while the condenser is set in the observation position, flare may be noticeable with certain specimens. If the transmitted DIC observation is not required, lower the condenser or use the light shield sheet @ provided with the vertical fluorescence illuminator.

  • Page 23: Troubleshooting Guide

    BX3-UCD8A TROUBLESHOOTING GUIDE Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact us for assistance.
  • Page 24 Problem Cause Remedy Page d) The ring slit does not match. A wrong optical element is engaged in Select the optical element turret the light path. matching the objective. A wrong objective is engaged. Engage the correct objective in the light path.
  • Page 25 BX3-UCD8A Problem Cause Remedy Page g) Interference color is not visible in DIC The polarizer is not engaged in the Engage the polarizer in the light path. observation. light path. Engage the analyzer in the light path. The analyzer is not engaged.

  • Page 26: Specifications

    SPECIFICATIONS Specification Item Dry top lens (U-TLD) Oil-immersed top lens (U-TLO) Applicable microscopes BX63, BX53, BX43 Applicable microscopy Transmitted light (Brightfield, darkfield, phase Transmitted light (Brightfield, DIC) contrast, DIC, polarizer light) Type Achromat, aplanat, motorized top lens swing-out (top lens interchangeable) NA (Number of Aperture) 0.9 (top lens IN)/ 1.4 (top lens IN) oil immersed/...

  • Page 27: Assembly

    BX3-UCD8A ASSEMBLY The motorized mechanisms will be damaged if they are moved by hand with excessive force. After connecting CAUTION the condenser as described in the BX3-CBH or U-CBM control box, set the main switches to “ I ” (ON) and control them from the specified control module.

  • Page 28: Mounting The Optical Elements

    6-2 Mounting the Optical Elements Optical Element Mounting Positions (Fig. 6) 1, 3: The U-DIC10S or U-DP10S optical elements can be mounted. When these positions are not used, the U-PH1S, U-PH2S and/or U-PH3S can be mounted. Any of the U-PH1S, U-PH2S or U-PH3S can be mounted. 4, 8: The U-DFA and/or U-PH3 can be mounted.
  • Page 29 BX3-UCD8A Combinations of Optical Elements and Objectives Per Observation Mode Phase contrast (PH) or darkfield (DF) observation: When the U-TLD top lens is used Optical Observation Applicable Objectives Element U-PH1S PLN10XPH, PLN20XPH, UPLFLN10X2PH, UPLFLN20XPH U-PH2S PLN40XPH, UPLFLN40XPH U-PH3S, U-PH3 PLN100XOPH, UPLFLN60XOPH, UPLFLN100XO2PH, UPLXAPO60XOPH, UPLXAPO100XOPH...
  • Page 30 DIC observation (U-DICT/U-DICTS) (Note) For the U-DICTHC/U-DICTHR, see next page. DIC slider U-DICT Shift type U-DICTS Top lens U-TLD U-TLO U-TLD U-TLO UPLFLN 10X2 U-DIC10, U-DIC10S ---- U-DIC10, U-DIC10S ---- U-DIC20 U-ODIC20 U-DIC20 U-ODIC20 U-DIC40 U-ODIC40 U-DIC40 U-ODIC40 U-DIC60 U-ODIC60 U-DIC60 U-ODIC60 60XO...
  • Page 31 BX3-UCD8A DIC observation (U-DICTHC/U-DICTHR) High-contrast type U- DIC slider High-resolution type U-DICTHR DICTHC Top lens U-TLD U-TLD U-TLO (for VEC/DIC) UPLFLN 10X2 U-DIC10HC U-DIC10HR ---- U-DIC20HC U-DIC20HR ---- U-DIC40HC U-DIC40HR ---- U-DIC60HC U-DIC60HR U-ODIC60HR 60XO 100XO2 U-DIC100HC U-DIC100HR U-ODIC100HR 100XO...
  • Page 32 Mounting the Phase Contrast Ring or Darkfield Ring (Figs. 7 to 9) 1. Set the top lens to the IN position with the motorized control. 2. Loosen the turret cover retaining screw @ using the provided Allen screwdriver and remove the turret cover 2. Be careful not to damage the top lens and the anti-dust glass CAUTION on the turret cover during its removal.
  • Page 33 BX3-UCD8A 5. Insert the phase contrast ring or darkfield ring into the turret position until Optical element it touches the bottom. When inserting, push the spring | on the inner side of the turret lightly with the side of the ring (Fig. 8).
  • Page 34 Mounting the DIC Prism (Figs. 10 & 11) } The mounting method is variable depending on whether the DIC prism is mounted in a position with centering mechanism (1, 3, 4 or 8) or in a position without (5, 6 or 7). Mounting on position 1, 3, 4 or 8 The DIC ring can be mounted in the same way as the phase contrast ring.
  • Page 35 BX3-UCD8A Mounting on position 5, 6 or 7 } The DIC prism should be clamped without using the built-in optical element centering screws. Instead, use the provided Allen wrench 2. 1. Engage the position for mounting the DIC prism in the light path by the...

  • Page 36: Installing The Condenser

    6-3 Installing the Condenser Install the condenser on the condenser holder as described below. Refer also to the instruction manual for the microscope frame. 1. When the microscope frame is the BX53/BX43, detach the revolving nosepiece, and raise the stage as high as possible. When the frame is the BX63, the above is not necessary because the stage height is fixed.
  • Page 37 Operation using BX63 (Fig.12) 8. Attach the cable holder @ to BX63. 9. Connect the interface cable 2 to BX3-UCD8A. 10. Twist the interface cable 2 once so that it bends toward the lower direction, and put it into the cable holder @.

  • Page 38: Centering The Condenser

    6-4 Centering the Condenser } The condenser centering screw on the right can be accessed by inserting the Allen screwdriver from the condenser centering screw guide hole, and that on the left can be accessed directly with the Allen screwdriver. Adjust centering of the condenser as described below.
  • Page 40 Distributed by Evident Europe GmbH Caffamacherreihe 8-10, 20355 Hamburg, Germany Evident Europe GmbH – UK Branch Part 2nd Floor Part A, Endeavour House, Coopers End Road, Stansted CM24 1AL, UK Evident Scientific, Inc. 48 Woerd Ave, Waltham, MA 02453, USA Evident Scientific Singapore PTE.

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