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Evident BX43 Instructions Manual

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Instructions
BX43
System microscope
To ensure safety, obtain optimum performance and to familiarize yourself fully with the use
of this product, we recommend that you study this manual thoroughly before operating this
Optical Microscope and Accessory
product, and always keep this manual reachable when operating this product.
For details of products included in the configuration of this microscope, see page 4.

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Summary of Contents for Evident BX43

  • Page 1 Instructions BX43 System microscope To ensure safety, obtain optimum performance and to familiarize yourself fully with the use of this product, we recommend that you study this manual thoroughly before operating this Optical Microscope and Accessory product, and always keep this manual reachable when operating this product.
  • Page 2 In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local our distributor in EU for return and/or collection systems available in your country.

  • Page 3: Table Of Contents

    BX43 Contents Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section “10 Assembly” (Page 34) carefully. Introduction ............................... 1 1 Module nomenclature ........................4 2 Controls .................................
  • Page 4 Rotating the stage ..................................21 Adjusting the stage height ..............................21 5-4 Observation tube .............................. 22 Adjusting the diopter ................................22 Using the eye shades ................................22 Mounting the eyepiece micrometer disk ......................23 Selecting the light path of the trinocular tube ................23 Adjusting the tilt ...................................24 Using eyepieces incorporating a micrometer ................25 5-5 Condenser ................................

  • Page 5: Introduction

    BX43 Introduction Safety precautions (Fig.1) 1. If potentially infectious samples may be observed, use protective gloves or other protective means to prevent the skin from contacting with samples directly. After observation, be sure to clean the portion contacted with samples.

  • Page 6: Getting Ready

    Restrictions in Use When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward diagonally backward 45°...

  • Page 7: Maintenance And Storage

    BX43 Maintenance and storage 1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

  • Page 8: Module Nomenclature

    Eyepieces · WHN10X · WHN10X-H Arm (or vertical illuminator) · BX3-ARM · BX3-URA* · BX3-RFAS* Lamp housing Revolving nosepiece · U-LHLEDC · BX43-5RES · U-LS30-5 · U-5RE-2 · U-D6RE Slide holder · U-HLST-4 · U-HRD-4 · U-HRDT-4 Stage · U-SVLB-4 ·...

  • Page 9: Controls

    BX43 Controls If you have not yet assembled the microscope, see “10 Assembly” on page 34. Condenser height adjustment Interpupillary distance adjustment knob (Page 12) scale (Page 11) Allen screwdriver holder Diopter adjustment ring (Page 22) Slide holder clamping lever (Page 19)
  • Page 10 Main switch (Page 10) : ON : OFF Pre-focusing lever (Page 16) LIM setting switch (Page 13) Fine adjustment knob (Page 16) LED brightness adjustment knob (Page 13) LIM indicator ON : Lit (Green) OFF : Extinguished LIM ON-OFF switch (Page 13)
  • Page 11 BX43 Swing-out condenser Low magnification condenser U-SC3 U-LC Top lens Top lens swing-out knob Auxiliary lens mounting tool (for BX53) Aperture iris diaphragm ring (Page 12) Auxiliary lens for U-LC Condenser mount dovetail * The auxiliary lens is mounted on the LED lamp housing (U-LHLEDC) or LS30 adapter (U-LS30ADP) when the U-LC is used, see page 35.

  • Page 12: Flow Of Observation

    Flow of observation · When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated. · When the halogen bulb is used, set the LBD filter in the filter mount on the base (or in the U-FC filter cassette). (Controls used) (Page) LED lamp...
  • Page 13 BX43 (trinocular tube only) Make a photocopy of the observation procedure pages and post it near your microscope.

  • Page 14: Simplified Observation Procedure

    Simplified observation procedure 4-1 Basic operation (until observation of specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each control, please read the description page specified below. Press the main switch of the microscope frame to “...

  • Page 15: Microscope Adjustments (How To Improve The Observed Image)

    BX43 Rotate the revolving nosepiece to engage the 10X objective in the light path. Rotate the coarse and fine adjustment knobs to bring the specimen in focus. (Details: Page 16) Rotate the stage knob to adjust the observation position. Now you can observe the magnified image of the specimen.

  • Page 16: Adjusting The Centering

    Adjusting the centering Place the specimen. Rotate the revolving nosepiece to select the 10X objective. Rotate the knobs to bring the specimen into focus. Rotate the knob to raise the condenser to its upper limit. Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field of view.

  • Page 17: Using The Controls

    BX43 Using the controls 5-1 Base Adjusting the brightness ( Figs.10 & 11) When the LED lamp is used for observation, turn the LED brightness adjustment knob a clockwise to make illumination brighter. When the halogen bulb is used for observation, adjust the brightness adjustment knob b of the TL4 power supply unit.

  • Page 18: Using The Filters (Halogen Bulb Operation Only)

    Using the filters (halogen bulb operation only) (Figs.13 to 17) You can place a filter in the light path with either method. · Place a filter on the filter mount on the base and engage in the light path. (Fig.13) ·...
  • Page 19 BX43 Using the filter cassette (Fig.17) Usable Filters Applications 45-LBD-IF* Color balancing filter 45-ND6, 45-ND25 Neutral density filter 45G-530, 43IF550-W45* Green B&W contrast filters 45O-560 Orange Up to three of the above filters can be loaded in the filter cassette.

  • Page 20: Focusing Block

    5-2 Focusing block Focusing controls (Figs.18 & 19) The focusing block consists of the controls listed in the following table, which also describes the functions of the controls. Name Function Coarse Moves the focus position widely. adjustment knob Fine adjustment Fine adjusts the focus position.

  • Page 21: Replacing The Fine Adjustment Knob

    BX43 Replacing the fine adjustment knob (Fig.21) CAUTION The fine adjustment knob has been attached on the right side at the factory. The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the X-and Y-axis knobs.

  • Page 22: Stage

    5-3 Stage Placing the specimen (Figs.24 & 25-A & 25-B) · The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. ·...

  • Page 23: Using The Auxiliary Slide Holder

    BX43 When using the specimen hold plate (CX3-SHP) For attaching procedures, see "Attaching specimen hold plate" (page 37). Rotate the coarse focusing knob a to lower the stage sufficiently. Place the specimen on the resin sheet b . CAUTION · As the outside of the aperture c of the resin sheet is not illuminated by the light from the condenser, the specimen Fig.25-B...

  • Page 24: Adjusting The X- And Y-Axis Knob Tension

    Adjusting the X- and Y-Axis knob tension (Fig.27) Hold the X-axis knob a and slide up the Y-axis knob b up to expose the adjustment knobs. Turning the X-axis adjustment knob c or Y-axis adjustment knob d clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it.

  • Page 25: Rotating The Stage

    BX43 Rotating the stage (Fig.28) Using the Allen screwdriver, slightly loosen the stage clamping screw The stage can be rotated both clockwise and counterclockwise by the stage clamping screw a . CAUTION A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunction.

  • Page 26: Observation Tube

    5-4 Observation tube Adjusting the diopter (Fig.31) Set the diopter adjustment rings on both sides to scale “0”. Engage a high-power objective (40X or so) in the light path, look into the right eyepiece with your right eye, and rotate the coarse and fine adjustment knobs to bring the specimen into focus.

  • Page 27: Mounting The Eyepiece Micrometer Disk

    BX43 Mounting the eyepiece micrometer disk (Fig.33) When the WHN10X-H eyepieces are used, an eyepiece micrometer disk can be mounted. Use 24 mm dia. x 1.5 mm thick micrometer disks. Following Fig.33, turn the built-in micrometer-mounting frame b to the arrow direction to remove it from the eyepiece and place a micrometer disk a into the mounting frame so that the surface with the model indication faces downward.

  • Page 28: Adjusting The Tilt

    Adjusting the tilt (Fig.35 to 38) 5° to 35° Adjust the height and tilt of the observation tube to obtain the most comfortable viewing position. U-TBI-3 5° to 35° U-TBI-CLI 5° to 35° U-ETBI 0° to 25° U-TTBI 0° to 25° U-TTR-2 5°...

  • Page 29: Using Eyepieces Incorporating A Micrometer

    BX43 Using eyepieces incorporating a micrometer (Fig.39) When the eyepieces in use incorporate a micrometer, the accuracy of the left-right focusing adjustment (diopter adjustment) can be improved further. Looking into the right eyepiece with your right eye, turn the top of the eyepiece a so that the micrometer in the field of view looks sharpest.

  • Page 30: Immersion Objectives

    5-6 Immersion objectives CAUTION Be sure to use the provided our Immersion oil. Using immersion objectives (Fig.40) Focus on the specimen with objectives in the order of lower-power to higher-power ones. Before engaging the immersion objective, apply a drop of provided immersion oil onto the specimen at the area to be observed.

  • Page 31: Camera Recording

    BX43 Camera recording · Use a trinocular observation tube such as the U-TTR-2 when recording images with camera. The trinocular tube accepts a camera adapter (certain camera adapters necessitate a camera mount adapter). · Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not match that of the image observed through eyepieces.

  • Page 32: Troubleshooting Guide

    Troubleshooting guide Under certain conditions, performance of the system may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact us for assistance. Problem Lamp Cause...
  • Page 33 BX43 Problem Lamp Cause Remedy Page Dirt or dust is visible in the field Clean thoroughly. Common Dirt/dust on the eyepieces. of view. Common Dirt or dust on condenser top lens. Common Dirt/dust on the specimen. Visibility is poor Common...
  • Page 34 Problem Lamp Cause Remedy Page Image cannot be focused: Common Tension adjustment ring is too Tighten ring. loose. · Objective drifts down by itself. · Fine adjustment knob slips. Coarse adjustment will not go Common Pre-focusing lever is locked at a Unlock pre-focusing lever.

  • Page 35: Specifications

    Upper coarse adjustment limit stopper Tension adjustment on coarse focus adjustment knob. 4. Revolving BX43-5RES U-D6RE Type nosepiece Coded 5 position nosepiece for BX43 Universal 6-position revolving nosepiece Transmitted light DIC slider Attachable module Transmitted light analyzer 5. Observation U-TBI-3/...
  • Page 36 Item Specification 7. Condenser U-LC U-AC2 U-SC3 U-AAC Type Low magnification Abbe Swing-out Achromat/ Aplanat Max. N.A. 0.75 1.10 1.40 1.25X (Widefield: FN 22) (Widefield: FN 22) 10X - 100X Applicable objective 2X - 60X 10X - 100X 2X - 100X (Super widefield: powers (Widefield: FN 22)

  • Page 37: Optical Characteristics (Uis2 Series)

    BX43 Optical characteristics (UIS2 series) The following table shows the optical characteristics Magnification of objective Objective series (PL is described as Plan) of combinations of eyepieces and objectives. Numerical aperture (NA) The figure on the right shows the performance data...

  • Page 38: Assembly

    WHN10X-H (FN 22) Intermediate attachment U-TTLBI SWH10X-H (FN 26.5) U-EPA2 U-TR30-2, etc. MICRO SWH10X (FN 26.5), etc. U-DO3 U-TRU, etc. Revolving nosepiece Reflected light BX43-5RES Observation tube mercury /xenon U-5RE-2 clamping screw lamp housing U-D6RE U-D7RES, etc. Vertical illuminator UIS2 series BX3-URA...

  • Page 39: Detailed Assembly Procedures

    BX43 10-2 Detailed assembly procedures Attaching the auxiliary lens for the U-LC condenser (Fig.42) When a condenser other than the U-LC is to be used, remove the auxiliary lens. Attach the auxiliary lens only when the U-LC is to be used.
  • Page 40 Installing the halogen bulb (Fig.45) Use only the designated bulb 6V30WHAL (PHILIPS 5761). Holding the bulb b a piece of gauze, insert the bulb pins c straight and fully into the pin holes d on the lamp socket. CAUTION To prevent reduced bulb life or cracking, do not touch the bulb with bare hands.
  • Page 41 BX43 Attaching the specimen holder (Fig.48 to 53) The attaching procedures are different between the regular specimen holder and the specimen hold plate (CX3-SHP). When using the regular specimen holder (Fig.48, 49) Rotate the coarse focusing knob to lower the stage sufficiently.

  • Page 42: Attaching The Eyepieces

    Place the metal plate c over the resin sheet (one sheet only) b by matching the hole positions of the metal plate with the hole positions of the resin sheet. Match the hole of the overlapped parts with the hole position of the stage plate and place the overlapped parts on the stage plate.

  • Page 43: Attaching The Revolving Nosepiece

    Use only coded 5 position nosepiece for BX43 (BX43-5RES) · Distribute its cable d toward the rear panel by passing it below the arm, and connect the cable to the connector no the rear panel.

  • Page 44: Attaching The Ac Adapter

    Attaching the AC adapter (Fig.58) CAUTION · Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force. · Make sure that the main switch is set to “ ” (OFF) before connecting the power cord. Insert the connector a of the AC adapter firmly into the connector b on the microscope.

  • Page 45: Preventive Inspection Sheet For Illumination Devices

    BX43 11 11 Preventive inspection sheet for illumination devices · We recommend performing “Preventive Inspections“ periodically (every time you replace lamps and at least once every 6 months). · The table below identifies the check items to be observed. Put (X) if not applicable or ( ) if applicable.

  • Page 46: Proper Selection Of The Power Supply Cord

    Proper selection of the power supply cord If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below: Caution : In case you use a non-approved power supply cord for our products, we can no longer warrant the electrical safety of the equipment.
  • Page 47 BX43 Table 2 HAR flexible cord Approval organizations and cordage harmonization marking methods Alternative marking utilizing Printed or embossed black-red-yellow thread (Length harmonization marking (May be of color section in mm) Approval organization located on jacket or insulation of internal wiring)

  • Page 48: Request To Issue The Safety Confirmation

    Evident. · Copy the Safety Confirmation shown on the next page, fill it out, and submit it to Evident maintenance personnel. · If the Safety Confirmation is not issued, we may not be able to provide maintenance, repairs or other services.
  • Page 49 Confirmation of Disinfection/sterilization of this product has not been disinfection/sterilization carried out. of product *We do not accept repairs/inspections of products used in the BSL4 environment. Facility name Department Signature Contact details Telephone, E-mail, etc. Evident Corporation...
  • Page 50 6666 Inatomi, Tatsuno-machi, Kamiina-gun, Nagano 399-0495, Japan Distributed by Evident Europe GmbH Caffamacherreihe 8-10, 20355 Hamburg, Germany Evident Europe GmbH – UK Branch UK Responsible Person Part 2nd Floor Part A, Endeavour House, Coopers End Road, Stansted CM24 1AL, UK Evident Scientific, Inc.

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