Evident CX23 Instructions Manual
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Evident CX23 Instructions Manual

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INSTRUCTIONS
CX23
Biological Microscope
With trinocular tube
This instruction manual is for the Olympus biological microscope.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating
this microscope, and always keep this manual at hand when operating this product.
Optical Microscope and Accessory
Retain this instruction manual in an easily accessible place near the work desk for future
reference.

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  • Page 1 INSTRUCTIONS CX23 Biological Microscope With trinocular tube This instruction manual is for the Olympus biological microscope. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating this microscope, and always keep this manual at hand when operating this product.
  • Page 2 In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.

  • Page 3: Table Of Contents

    Contents Safety precautions ............................. 1 Standard combination......................... 2 Nomenclature of operating portions ..................3 Outline of brightfield/darkfield observation methods ..........4 Observation procedures ........................Turning ON the LED illumination ............................Selection between the eyepiece light path and the camera light path ........Placing the specimen slide ...............................
  • Page 4 5 Glossary of optical performance terminology ............. 6 Troubleshooting ............................7 Specifications ............................8 List of optical performances ......................9 Assembly ............................... 9-1 Assembly diagram ............................27 9-2 Assembly procedures ..........................28 Removing the standard 10X eyepiece ........................Attaching the eyepiece micrometer .......................... Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H) ...........

  • Page 5: Safety Precautions

    CX23 Safety precautions If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product may also be damaged. Always use the product according to this instruction manual.
  • Page 6 CAUTION - LED (light emitting diode) Do not look directly at the light from LED for a long time. If you feel that the light from LED is too bright during observation, adjust the light intensity using the brightness adjustment knob and continue observation. The LED built in this product is basically eye-safe. However, do not look directly at the light from LED for a long time while feeling too bright, since it may cause damage to your eyes.
  • Page 7 CX23 Handling precautions · This product is a precision instrument. Handle it with care NOTE and avoid subjecting it to a sudden or severe impact. · Never disassemble any part of the product. Otherwise, failure could be caused. · The objectives are screwed in tightly to prevent them from being loosened during transportation.
  • Page 8 Maintenance and storage Do not leave stains or fingerprints on the lenses and filters. If they get dirty, blow away dust with a commercially available blower and gently wipe the lens or filter with a piece of cleaning paper (or clean gauze). Only when cleaning fingerprints and oil stains, slightly moisten a piece of cleaning paper with commercially available absolute alcohol and wipe them off with it.

  • Page 9: Standard Combination

    CX23 Standard combination Refer to the drawing below and make sure that all necessary components are included in the product you purchased. Tube Eyepieces Objective Power cord Revolving nosepiece Stage Condenser Auxiliary lens unit Field diaphragm unit AC adapter Microscope...

  • Page 10: Nomenclature Of Operating Portions

    Nomenclature of operating portions Pre-focusing knob Binocular portion Light path selection knob Tube fixing knob Condenser height Condenser height Diopter adjustment knob adjustment knob adjustment rings Revolving nosepiece Specimen holder Y-axis knob Aperture Tension adjustment ring Tension adjustment ring diaphragm lever Auxiliary lens Auxiliary lens Coarse focusing knob...

  • Page 11: Outline Of Brightfield/Darkfield Observation Methods

    CX23 Outline of brightfield/darkfield observation methods (Operation portion) (Page) Main switch 9  Set the main switch to (ON) and adjust the brightness. Brightness adjustment knob Light path selection knob Select the light path. (Trinocular tube) Specimen holder Place the specimen slide on the stage.
  • Page 12 7 7 7 Make a copy of this observation procedure guide and put it near the microscope to use for observation.

  • Page 13: Observation Procedures

    CX23 Observation procedures Turning ON the LED illumination Set the main switch a to (ON). Rotating the brightness adjustment knob b in the arrow direction increases the brightness and rotating it in the opposite direction decreases the brightness. The values shown around the knob represent the rough indication of brightness.

  • Page 14: Placing The Specimen Slide

    Placing the specimen slide Rotate the coarse focusing knob a in the arrow direction to fully lower the stage. Press the holding lever knob b backward (arrow direction) to open the specimen holding lever c , and place the specimen slide d by sliding it on the stage from front toward back.
  • Page 15 CX23 Stage operation in X/Y direction Scale of specimen position By reading and taking a note of X-axis and Y-axis scales (coordinates) of the specimen observation position on the specimen slide, you can return to the original observation position easily even if the specimen is moved.

  • Page 16: Selecting The Objective

    Selecting the objective Hold the revolving nosepiece a and rotate it so that the intended objective comes exactly above the specimen slide. · Do not rotate the revolving nosepiece by holding the NOTE objective. · Be careful if you rotate the revolving nosepiece while observing the edge of the specimen slide with the high magnification objective (40X, etc.), the objective may interfere with the specimen holder.

  • Page 17: Adjusting The Interpupillary Distance

    CX23 Using the pre-focusing lever The pre-focusing lever prevents the specimen slide from being damaged by collision between the specimen slide and objective. After bringing the specimen into focus with the objective which has the shortest WD, rotate the pre-focusing lever a clockwise until it stops.

  • Page 18: Adjusting The Diopter

    Adjusting the diopter. The diopter adjustment is to compensate for the difference in eyesights of left and right eyes of the observer. Rotate the diopter adjustment rings a of the right and left eyepieces and move each scale “0” to each index b . Engage the 10X objective in the light path and rotate the coarse/fine focusing knobs to bring the specimen into focus.

  • Page 19: Adjusting The Condenser Position

    CX23 Using the eye shades When wearing eyeglasses Use the eye shades in the normal, folded-down position. When not wearing eyeglasses Extend the folded eye shades in the arrow direction. Since the eye shades prevent the unnecessary light from entering between eyepieces and eyes, you can observe the specimen comfortably.

  • Page 20: Adjusting The Field Diaphragm (Fs)

    Adjusting the field diaphragm (FS) The field diaphragm is a diaphragm to adjust the illumination area of the specimen. By adjusting the illumination area slightly wider than the field of view depending on objectives to be used, the excess light can be blocked to acquire the image with good contrast.
  • Page 21 CX23 Rotate the field diaphragm ring f in the direction shown in the picture on the left to narrow down the field diaphragm. (Picture A shown below) Field diaphragm image Eyepiece Field of view The field diaphragm image in view is slightly blurred.

  • Page 22: Attaching The Darkfield Ring Slit (For Darkfield Observation Only)

    Attaching the darkfield ring slit (for darkfield observation only) For darkfield observation, the darkfield ring slit CH2-DS (option) must be attached to the condenser. Remove the auxiliary lens unit a attached to the condenser. When removing the auxiliary lens unit a , be sure to hold NOTE it and push it down.

  • Page 23: Observation

    CX23 Observation Engage the objective to be used in the light path and bring the specimen into focus. (See page 12.) Rotate the brightness adjustment knob a to adjust the brightness of the LED illumination. (See page 9.) Acquiring the image with the camera The observed image can be acquired by attaching the camera adapter and the digital camera for microscope to the trinocular tube.

  • Page 24: Using The 100X Oil Immersion Objective

    Using the 100X oil immersion objective · Apply the immersion oil specified by Olympus to the tip of the 100X oil immersion objective. NOTE Otherwise, the observed image is not in focus. · Always use the immersion oil provided by Olympus. Using the immersion oil which is not provided by Olympus cannot deliver the intended optical performance.

  • Page 25: Glossary Of Optical Performance Terminology

    CX23 Glossary of optical performance terminology Total magnification The size of the specimen image to be observed is obtained by multiplying the eyepiece magnification by the objective magnification. This value is referred to as the total magnification. Example: Eyepiece (10X) x Objective (40X) = 400X Resolution The resolution is the ability of the lens to separate the image created by multiple proximal points.
  • Page 26 Aperture diaphragm The aperture diaphragm is a diaphragm to adjust the numerical aperture of the condenser. Adjusting the numerical aperture of the condenser appropriately with respect to the numerical aperture of each objective allows you to observe the specimen with the best contrast and resolution. In general, since the contrast of the specimen to be 70-80% observed with microscope is low, it is appropriate to adjust...

  • Page 27: Troubleshooting

    CX23 Troubleshooting If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Olympus for assistance. Problem Cause Remedy Page The LED illumination does not...
  • Page 28 Problem Cause Remedy Page One-sided blur is found. The objective is not engaged in the Rotate the revolving nosepiece until it The image looks as if flowing. light path properly. clicks to engage the objective in the light path. The specimen slide is not placed on Place the specimen slide on the the stage properly.

  • Page 29: Specifications

    CX23 Specifications Item Specification Optical system Infinity optical system Microscope frame CX23RTFS2 / CX23LTFS2 Illumination system Built-in 0.5 W LED illumination system Microscope frame (rated input power): 5-6 V 0.5 A AC adapter (rated input power): 100-240 V $ 50-60 Hz 0.4 A AC adapter (rated output power): 5 V 2.5 A...

  • Page 30: List Of Optical Performances

    List of optical performances Magnification The following table shows the optical performances when combining eyepieces and Numerical aperture objectives. Mechanical tube The picture on the right shows the various length performances indicated on the objectives. Objective field number * Color band Cover glass thickness Objective type (on the back side) * “FN”...

  • Page 31: Assembly

    CX23 Assembly 9-1 Assembly diagram The number in the following diagram indicates the order to attach each unit. The detail assembly procedures are described on and after next page. Camera DP22 / DP27 (Option) Camera adapter Standard 10X eyepiece U-TV1XC...

  • Page 32: 9-2 Assembly Procedures

    9-2 Assembly procedures Removing the standard 10X eyepiece The standard 10X eyepieces are clamped with screws. Loosen the clamping screws a of the 10X eyepieces using the small flathead screwdriver and remove the eyepieces. Attaching the eyepiece micrometer Use the eyepiece micrometer of 24 mm in diameter and 1.5 mm in thickness.

  • Page 33: Attaching The Eyepieces (Standard 10X Eyepieces Or Whsz15X-H)

    CX23 Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H) Insert WHC15X-H into the eyepiece sleeves and tighten the clamping screws a using the flathead screwdriver. Attaching the objective CXPL20X or CXPL100XO Screw in the objective to the mount hole of the revolving nosepiece completely by rotating in the arrow direction paying attention not to drop it.

  • Page 34: Attaching The Auxiliary Lens Unit And Field Diaphragm Unit

    Attaching the auxiliary lens unit and field diaphragm unit For details, see page 16. Attaching the filter holder CH2-FH and darkfield ring slit CH2-DS Insert the darkfield ring slit CH2-DS a into the filter holder CH2-FH b . One filter of 32.5mm in diameter can be inserted to the filter holder in addition to the darkfield ring slit.

  • Page 35: Connecting The Ac Adapter And Power Cord

    CX23 Connecting the AC adapter and power cord · Always use the AC adapter and power cord provided by Olympus. If the proper AC adapter and the CAUTION power cord are not used, the electric safety and the EMC (Electro-Magnetic Compatibility) performance of the product cannot be assured.

  • Page 36: Attaching The Allen Wrench Holder

    Attaching the allen wrench holder · It is recommended to attach the provided allen wrench holder a to the back of the microscope frame. · The provided allen wrench b can be fixed to the provided allen wrench holder a .
  • Page 37 CX23 Proper selection of the power supply cord If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below: Caution : In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 38 Table 2 HAR flexible cord Approval organizations and cordage harmonization marking methods Alternative marking utilizing Printed or embossed black-red-yellow thread (Length harmonization marking (May be of color section in mm) Approval organization located on jacket or insulation of internal wiring) Black Yellow Comite Electrotechnique Belge...
  • Page 40 Distributed by EVIDENT EUROPE GmbH Caffamacherreihe 8-10, 20355 Hamburg, Germany EVIDENT EUROPE GmbH UK Branch Part 2nd Floor Part A, Endeavour House, Coopers End Road, Stansted CM24 1AL, U.K. OLYMPUS SCIENTIFIC SOLUTIONS AMERICAS CORP. 48 Woerd Ave Waltham, MA 02453, U.S.A.

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