Related Manuals for KERN Optics OBN-14
Summary of Contents for KERN Optics OBN-14
- Page 1 Fluorescence microscope OBN-14 OBN 141, OBN 142, OBN 147, OBN 148 PROFESSIONAL MEASURING english version Fluorescence microscope operating instructions Version 2024-09 OBN-14-BA-en-2411.docx...
- Page 2 You can find other language Further language versions Más versiones de idiomas versions online at you will find online under se encuentran online bajo www.kern- www.kern- www.kern- sohn.com/manuals sohn.com/manuals sohn.com/manuals Inne wersje językowe znajdą Vous trouverez d'autres Trovate altre versioni di Państwo na stronie versions de langue online lingue online in...
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Page 3: Table Of Contents
KERN Optics OBN-14 Fluorescence microscope Fluorescence microscope operating instructions Version 2024-09 english version Table of contents: Technical data ........................... 3 Declaration of conformity ......................4 Overview of the device......................6 Nomenclature ..........................6 Rear view ............................ 8 Before use ..........................10 General information ........................ - Page 4 Service ............................. 56 Power supply .......................... 57 16.1 Mains connection ........................57 Maintenance, servicing and disposal ................... 58 17.1 Cleaning ........................... 58 17.2 Maintenance and repair ......................58 17.3 Waste disposal ......................... 59 Further information ........................ 60 2 | en OBN-14-BA-en-2411.docx...
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Page 5: Technical Data
5V, 1A 50/60Hz 2A Plug-in power supply Plug-in power supply type Built-in power supply unit Fuse 2A 5x20mm Focusing mechanism Coaxial coarse and fine drive Packaging dimensions 430x220x490 mm Net weight 16 kg Gross weight 24 kg OBN-14-BA-en-2411.docx en | 3... -
Page 6: Declaration Of Conformity
Declaration of conformity The current EC/EU Declaration of Conformity can be found online at: https://www.kern-sohn.com/shop/de/DOWNLOADS/ 4 | en OBN-14-BA-en-2411.docx... - Page 7 NOTE: The fluorescence microscopes of the OBN-14 series can be divided into two main components: • Transmitted light microscope (KERN Professional Line) • Fluorescence incident light unit The following operating instructions initially relate purely to the transmitted light microscope itself.
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Page 8: Overview Of The Device
Overview of the device Nomenclature 6 | en OBN-14-BA-en-2411.docx... - Page 9 X - Y axis Object table ON/OFF switch Dimmer Fine drive Coarse drive Adjusting screw Condenser Centering screw Condenser Field lens with Luminous field diaphragm Centering screw Condenser Aperture setting Condenser Condenser Object table Object holder Objective Nosepiece OBN-14-BA-en-2411.docx en | 7...
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Page 10: Rear View
Rear view 8 | en OBN-14-BA-en-2411.docx... - Page 11 Coarse drive Fine drive Mains connection (for OBN 141/142) Mains connection and fuse (for OBN 147/148) Main switch (for OBN 147/148) Carrying handle Condenser Focus wheel Adjustment ring Torque Main switch (for OBN 141/142) and dimmer OBN-14-BA-en-2411.docx en | 9...
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Page 12: Before Use
If the performance of the microscope is to be maintained, it must never be disassembled. Parts such as objective lenses and other optical components should therefore be left as they are at the start of operation. 10 | en OBN-14-BA-en-2411.docx... -
Page 13: Basic Information (General)
The symbol indicates the type of hazard. Indicates general hazards or a danger point Warning of electrical voltage Warning of optical radiation Warning of explosive substances Warning of flammable substances Indicates electrostatic sensitive devices OBN-14-BA-en-2411.docx en | 11... -
Page 14: Intended Use
Indicates a prescribed action Intended use The OBN-14 is used in particular for the visualization of cell components of sophisticated preparations (e.g. living mammalian cells, tissue, possibly also microorganisms, immunofluorescence, FISH, DAPI staining, etc.). Important applications include the specific staining of proteins with fluorescently labeled ligands, antibodies or fluorescent proteins. -
Page 15: Basic Warnings And Safety Instructions
● Similarly, the lamp must not be switched on again immediately after it has been switched off. ● During a pause in observation, the control lever for the lighting should always be pushed in to interrupt the light beam. OBN-14-BA-en-2411.docx en | 13... - Page 16 ● Never look into the eyepieces when the beam path is open (using the control lever for illumination) and an empty filter position is selected on the FL module. There is an acute risk of blindness here. 14 | en OBN-14-BA-en-2411.docx...
- Page 17 ● An HBO lamp has a certain service life. The closer it is brought to its limit, the greater the risk of the lamp exploding and releasing toxic mercury vapor. This must be prevented by all means. OBN-14-BA-en-2411.docx en | 15...
- Page 18 Do not use the device in environments with high humidity or water mist NOTE ● To avoid damaging the device, do not expose it to extreme temperatures, extreme humidity or moisture. ● Do not use harsh cleaners, abrasive cleaners or solvents to clean the appliance. 16 | en OBN-14-BA-en-2411.docx...
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Page 19: Transportation And Storage
• Only the original packaging is to be used for return transportation. • Disconnect all connected cables and loose/movable parts before shipping. • Refit any transportation locks provided. • Secure all parts against slipping and damage. OBN-14-BA-en-2411.docx en | 17... -
Page 20: Unpacking And Commissioning
If any damage is evident, please notify KERN GmbH immediately. Initial commissioning To ensure the function of the microscope, it must be cleaned as described in chapter 18 | en OBN-14-BA-en-2411.docx... -
Page 21: Assembly
When doing this, you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures. OBN-14-BA-en-2411.docx en | 19... -
Page 22: Objectives
When doing this, you should be able to read the scale from the front. You should avoid touching the optical lenses with bare fingers. For points 9.6 (polarization unit) and 9.7 (camera connection), see 13 Use of optional accessories. 20 | en OBN-14-BA-en-2411.docx... -
Page 23: Operation
To move the sample into the beam path, the adjustment wheels on the right-hand side of the mechanical stage must be operated accordingly (see illustration on the right). A total of two slides can be placed at the same time. OBN-14-BA-en-2411.docx en | 21... -
Page 24: Pre-) Focusing
Important In order to avoid damaging to the focussing system, the left and right adjustment wheels for the coarse and fine adjustment knob must never be rotated at the same time in opposite directions. 22 | en OBN-14-BA-en-2411.docx... -
Page 25: Adjusting The Interpupillary Distance
Then look through the left eyepiece with the left eye and use the lefthand dioptre adjustment ring to focus the image. To do this, you just need to turn the ring in both directions (see illustration), to find out where the image is at its most focussed. OBN-14-BA-en-2411.docx en | 23... -
Page 26: Adjusting The Magnification
If you have focussed the object to be observed for a specific magnification, then if you select the objective with the next greatest magnification, then the object will be slightly out of focus. Use the fine adjustment knob to make a slight adjustment and restore the focus 24 | en OBN-14-BA-en-2411.docx... -
Page 27: Adjusting The Koehler Illumination
If, as with the devices in the KERN OBN-14 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 28 5. Open the field diaphragm until it just disappears out of the field of view. If necessary, simply re-centre using the centring screws on the condenser holder. 26 | en OBN-14-BA-en-2411.docx...
- Page 29 If another magnification is selected afterwards, then the Koehler illumination does not have to be reset from scratch, only the aperture diaphragm and field diaphragm need to be adjusted as required. As a result you can always check whether the condenser needs to be re-centred OBN-14-BA-en-2411.docx en | 27...
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Page 30: Using The Eyecups
In order to maintain hygiene, we would therefore recommend that you clean the eye cups regularly (e.g. with a damp cloth). Eyecups High Eye Point eyepiece (recognizable by the glasses symbol) 28 | en OBN-14-BA-en-2411.docx... -
Page 31: Use Of Oil Immersion Lenses
10.8 Use of oil immersion lenses The 100x objectives of the OBN-14 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. -
Page 32: Fluorescence Incident Light Unit
The barrier filter finally eliminates all wave ranges out of the beam path not belonging to the observed fluorescence. Thus the resulting image is just developed by the fluorescent light emitted from the sample. 30 | en OBN-14-BA-en-2411.docx... - Page 33 Overview of the wavelengths for excitation and emission per excitation filter Blue line: Wavelength excitation Green line: wavelength emission OBN-14-BA-en-2411.docx en | 31...
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- Page 36 Fixing Allen screw for microscope head Main body Connection point for microscope head Fixing screw for lamp housing HBO lamp housing Power connection Screw for mounting the protective plate Connection point for microscope head housing Allen wrench 34 | en OBN-14-BA-en-2411.docx...
- Page 37 Adjustment lever for field diaphragm Centering device for aperture diaphragm Adjustment lever for aperture diaphragm Condenser control Screw for cover of lamp housing Centering screw for lamp (vertical) Centering screw for lamp (horizontal) Control lever for illumination OBN-14-BA-en-2411.docx en | 35...
- Page 38 Nomenclature (power supply unit for HBO lamp) Description Operating time Ampere meter Main switch 36 | en OBN-14-BA-en-2411.docx...
- Page 39 Nomenclature (control elements) for OBN 141/142 Description 3W epi-fluorescence unit ( B/G) with OBN 141 3W epi-fluorescence unit ( B/G/UV/V) with OBN 142 Input socket Connection cable for fluorescence unit LED switch lever (B/G) LED changeover wheel (B/G/UV/V) OBN-14-BA-en-2411.docx en | 37...
- Page 40 Nomenclature (power supply unit for LED lamp: OBN 141/ OBN 142 5W LED Power Box (front) DC input LED Power Box (rear) DC input LED power cable Description ON/OFF switch Dimmer External power connection LED powerbox output socket DC/AC adapter Connection cable for fluorescence unit 38 | en OBN-14-BA-en-2411.docx...
- Page 41 HBO lamp must first be mounted in the lamp housing. For the procedure, see "Replacing the bulb" chapter 11 (Remove the previously fitted bulb transport lock for this). To open the lamp housing, loosen the hexagon socket screw (10) with a hexagon wrench. OBN-14-BA-en-2411.docx en | 39...
- Page 42 Loosen the fixing screw for the microscope head using the Allen key. Insert the connection point of the microscope head (8) into the opening provided in the centerpiece (9). Secure the microscope head with the locking screw using an Allen key. The microscope is now fully assembled. 40 | en OBN-14-BA-en-2411.docx...
- Page 43 4. Once the sample has been placed, the desired objective can be brought into the beam path. 5. Move the FL module control wheel to the desired position. 6. When using an HBO lamp, the control lever for the lighting can now be pulled out. 7. Start of observation. OBN-14-BA-en-2411.docx en | 41...
- Page 44 Operating time display on the power supply unit As soon as 100 h are reached→ Lamp replacement required • Before putting a new lamp into operation, press the reset button on the power supply unit next to the ammeter with a pointed object. 42 | en OBN-14-BA-en-2411.docx...
- Page 45 4. When looking through the eyepieces, a crosshair and the off-centered light spot of the lamp (also cross-shaped) appear. 5. Now use the two centering screws on the lamp housing to move the light spot into the center of the crosshairs (caution: heat build-up on the lamp housing). OBN-14-BA-en-2411.docx en | 43...
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Page 46: Lamp Replacement
The new bulb may only be handled with sterile gloves or with the aid of its packaging film in order to insert it into the holder. Grease or dust residues can have a negative impact on the light quality and service life. Halogen bulb Screw for bulb 44 | en OBN-14-BA-en-2411.docx... - Page 47 4. Pull the upper part of the housing upwards until it separates from the lower part ( see illustration) 5. The bracket in which the lamp is to be installed is located in the upper part. OBN-14-BA-en-2411.docx en | 45...
- Page 48 Due to the long service life of LED lighting, regular lamp replacement will not be necessary with this microscope. In most cases, problems with the lighting would therefore be caused by defects in the electrical system. In such a case, our Technical Service can help. 46 | en OBN-14-BA-en-2411.docx...
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Page 49: Fuse Replacement (Obn 147 / Obn 148)
It is advisable to use a screwdriver or similar tool to help with this. The defective fuse can now be removed from its housing and replaced with a new one. The fuse housing must then be reinserted into the slot below the mains plug connection OBN-14-BA-en-2411.docx en | 47... -
Page 50: Use Of Optional Accessories
The aperture setting of the condenser must be in position PH. For its starting position, the polariser must be turned to the position in which you can see the highest level of darkness in the field of view (without object slide). 48 | en OBN-14-BA-en-2411.docx... -
Page 51: Camera Connection
13.2 Camera connection Due to the trinocular tube, which is a standard fitting for the whole OBN-14 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed. -
Page 52: Phase Contrast Units
PH slider is now in the beam path. If the aperture setting on the PH condenser is now set to "PH", the phase contrast application can begin. To return to the bright field application, the PH slider must be moved further 50 | en OBN-14-BA-en-2411.docx... - Page 53 The position of the ring in the objectives cannot be altered, that of the rings in the condenser disc however can be. OBN-14-BA-en-2411.docx en | 51...
- Page 54 Depending on the preferences of the user, using a green filter can produce a more effective and pleasant image. To achieve this, the green filter must be screwed onto the underside of the PH condenser. 52 | en OBN-14-BA-en-2411.docx...
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Page 55: Troubleshooting
The diopter setting was not made correctly The field of vision of one eye does not Different eyepieces are used on the right and match that of the other eye left The eyes are not used to microscopy OBN-14-BA-en-2411.docx en | 53... - Page 56 The coarse drive is difficult to turn The cross table is operated by a Solid body blocked. The table moves down by itself The adjusting wheel/torque is not tightened The fine adjustment drive adjusts itself enough 54 | en OBN-14-BA-en-2411.docx...
- Page 57 Touching the table blurs the image The table was not assembled correctly OBN-14-BA-en-2411.docx en | 55...
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Page 58: Service
If, despite studying these operating instructions, you still have questions about commissioning or operation, or if, contrary to expectations, a problem should occur, please contact your specialist dealer. The device may only be opened by trained service technicians authorized by KERN. 56 | en OBN-14-BA-en-2411.docx... -
Page 59: Power Supply
( sticker ) and the local mains voltage are identical. Important: ▪ Check the mains cable for damage before commissioning ▪ Ensure that the power supply unit does not come into contact with liquids ▪ The mains plug must be accessible at all times. OBN-14-BA-en-2411.docx en | 57... -
Page 60: Maintenance, Servicing And Disposal
If handled correctly and checked regularly, the microscope will function smoothly for many years. 17.2 Maintenance and repair Do not make any changes to the device and do not install any spare parts. Contact the manufacturer for repair or device inspection. 58 | en OBN-14-BA-en-2411.docx... -
Page 61: Waste Disposal
• Electronic components (printed circuit boards, electrical cables • Plastic (housing) • Metal Improper disposal of the appliance can have harmful effects on people and the environment. Proper and environmentally friendly disposal can prevent harmful effects and recover raw materials. OBN-14-BA-en-2411.docx en | 59... -
Page 62: Further Information
The illustrations may differ slightly from the product. The descriptions and illustrations in these operating instructions are subject to change without notice. Further developments to the device may result in such changes. language versions include non-binding translation. The original German document is binding. 60 | en OBN-14-BA-en-2411.docx...
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