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Related Manuals for KERN Optics OBN 132
Summary of Contents for KERN Optics OBN 132
- Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light laboratory microscope KERN OBN-13 OBN 132, OBN 135 Version 1.0 01/2015 OBN-13-BA-e-1510...
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Page 3: Table Of Contents
KERN OBN-13 Version 1.0 01/2015 User instructions Transmitted light microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............8 Assembly .................. -
Page 4: Before Use
1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments. -
Page 5: Storage
Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down. -
Page 6: Maintenance And Cleaning
1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth. -
Page 7: Nomenclature
2 Nomenclature Tube connectors with dioptre adjustment ring Eyepiece Camera adapter connection with adjusting screw Microscope head / Tube Trinocular toggle rod Nosepiece Objective Adjusting screw Microscope head Object holder Specimen Control dial stage X – Y axis Condenser Specimen stage Aperture adjustment... - Page 8 Rear view Carrying handle Coarse adjustme nt knob Fine adjustment knob Main switch Fuse Power connection Adjusting ring torque Condenser Focus dial OBN-13-BA-e-1510...
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Page 9: Technical Data / Features
3 Technical data / Features Standard configuration Modell Optical system Tube Illumination KERN OBN 132 Infinity Trinokular 6V / 20W Halogen (Transmitting) OBN 135 Infinity Trinokular 3W LED (Transmitting) Eyepieces: WF 10x / Ø 20 mm Objectives: 4x / 10x / 20x / 40x / 100x... - Page 10 OBN-13-BA-e-1510...
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Page 11: Assembly
4 Assembly OBN-13-BA-e-1510... -
Page 12: Microscope Head
4.1 Microscope head First you must loosen the fixing screw on the tube connection point and remove the black protective cover. You can then insert the round dovetail bracket on the head into the round dovetail bracket on the housing and fix it with the fixing screw. When doing this, you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures. -
Page 13: Condenser
4.5 Condenser We recommend that you use the course adjustment knob to bring the specimen stage to its uppermost position. Use the focus dial of the condenser to move the condenser holder to the central position. In this way the condenser can be fitted at the right place in the condenser holder and fixed with the adjusting screw. -
Page 14: Pre-) Focussing
5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration). -
Page 15: Adjusting The Interpupillary Distance
5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly. -
Page 16: Adjusting The Magnification
5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path. -
Page 17: Adjusting The Koehler Illumination
5.6 Adjusting the Koehler illumination To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. If, as with the devices in the KERN OBN-13 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 18 3. Adjust the height of the condenser until the image from the field diaphragm appears clearly in the field of view. For some microscopes there is a risk that you will lift the condenser up so high that it collides with the object holder. Therefore care is needed when doing this.
- Page 19 6. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image. The scale divisions on the condenser can be used as a guideline. Select in accordance with the objective being used. The view in the tube without the eyepiece should look something like the illustration on the right.
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Page 20: Using Eye Cups
5.7 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces. -
Page 21: Using Oil Immersion Objectives
5.8 Using oil immersion objectives The 100x objectives of the OBN-13 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. 1. -
Page 22: Changing The Bulb
6 Changing the bulb Halogen You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt. Before changing the bulb the device must be switched off and unplugged. To change the bulb, tip the device carefully to the back or side. -
Page 23: Using Optional Accessories
8 Using optional accessories 8.1 Polarisation unit The polarisation unit consists of a polariser and an analyser. Both consist of a round glass plate with an external holder ring. To mount the analyser there is a slider with a socket fitted on the front of the microscope directly below the nosepiece (see illustration on page 10), where you can simply insert the analyser (see left illustration). -
Page 24: Camera Connection
8.2 Camera connection Due to the trinocular tube, which is a standard fitting for the whole OBN-13 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed. After the plastic cover has been removed from the camera adapter connector on the top of the microscope head, then a suitable adapter must be fitted. -
Page 25: Phase-Contrast Units
8.4 Phase-contrast units Fundamentally there are two different ways for you to conduct the phase-contrast procedure. A simple method and a more comprehensive method. 1. Simple phase-contrast unit This consists of a simple PH condenser, a PH objective with a specific magnification (10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a centring telescope and two green filters. - Page 26 With the surface marked “TOP” facing up, the PH slider is pushed upwards into the insertion point on the PH condenser until it clicks into the first position. At this first position, the phase ring of the PH slider is in the beam path. If the aperture setting on the PH condenser is set to “PH”, then the phase-contrast application can begin.
- Page 27 The adjustment for this is carried out as follows. a. First adjust the microscope using bright field mode. b. Move the hub to the required PH position (e.g. “10x”). c. Open the aperture diaphragm on the condenser to its maximum (slider to the left).
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Page 28: Fluorescence Reflected Light Unit
8.5 Fluorescence reflected light unit There are samples, which can be excited by light beams and thereby show emissions, which have different wave lengths than the previous excitation beams. The wave length of the emission is always bigger than the wave length of the excitation (Stokes shift). - Page 29 Overview of wave lengths for excitation and emission per excitation filter Blue line: Wave length excitation Green line: Wave length emission OBN-13-BA-e-1510...
- Page 30 OBN-13-BA-e-1510...
- Page 31 OBN-13-BA-e-1510...
- Page 32 Nomenclature (components) Protective plate HBO/LED lamp housing Cover for FL module Power connection Fixing Allen screw for microscope head Screw for attaching the protective plate Main body Connection point for microscope housing Connection point for microscope head Allen wrench Fixing screw for lamp housing OBN-13-BA-e-1510...
- Page 33 Nomenclature (control elements) Control wheel for FL module Condenser control Centring device for field diaphragm Screw for cover of lamp housing Control lever for field diaphragm Centring screw for lamp (vertical) Centring device for aperture diaphragm Centring screw for lamp (horizontal) Control lever for aperture diaphragm Control lever for illumination OBN-13-BA-e-1510...
- Page 34 Nomenclature (Power supply unit for HBO lamp) Basic data There are three different fluorescence reflected light units available for the microscopes of the series OBN-13. They have the following specifications. Article Illumination Excitation Centring Input voltage number filter objective power supply unit OBB-A1155 100W HBO UV / V / B / G...
- Page 35 Assembly In order to assemble the fluorescence reflected light unit correctly and mount it afterwards to the microscope housing, the following steps have to be carried out. 1. When the microscope is already assembled without the FL unit, you have to remove the microscope head at first.
- Page 36 Control elements for illumination The following control elements for the illumination play a role within the fluorescence microscopy: Field diaphragm, aperture diaphragm, condenser: Optimisation of the contrast and the light yield. Control lever for illumination: When located at the medium position there is a filter, which softens the lighting, so that samples, which are not stable enough for the full illuminating power, can be observed as well.
- Page 37 Centring the lamp (HBO) When running the HBO lamp for a long time there is the possibility, that the holder of the lamp is moving out of its centre due to the strong heat development. This circumstance has to be corrected when it appears, because therefore the field of view is no longer equally illuminated.
- Page 38 Changing the fuse The fuse housing is on the rear of the power supply unit next to the mains power supply socket. With the device switched off and unplugged, you can screw out the housing. The defective fuse can be removed from its housing and be replaced with a new one.
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Page 39: Trouble Shooting
9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set... - Page 40 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
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Page 41: Service
10 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.
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