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Related Manuals for KERN Optics OBN 159
Summary of Contents for KERN Optics OBN 159
- Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light phase contrast microscope KERN OBN-15 OBN 158, OBN 159 Version 1.1 01/2023 OBN-15-BA-e-2311...
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Page 2: Table Of Contents
KERN OBN-15 Version 1.1 01/2023 User instructions Phase contrast microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............8 Assembly .................. -
Page 3: Before Use
1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments. -
Page 4: Storage
Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down. -
Page 5: Maintenance And Cleaning
1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth. -
Page 6: Nomenclature
2 Nomenclature OBN-15-BA-e-2311... - Page 7 Rear view Carrying handle Coarse adjustme nt knob Fine adjustment knob Main switch Fuse Power connection Adjusting ring torque Condenser Focus dial OBN-15-BA-e-2311...
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Page 8: Technical Data / Features
Optical system Tube Illumination KERN OBN 158 Infinity Trinokular 6V / 20W Halogen (Transmitting) OBN 159 Infinity Trinokular 3W LED (Transmitting) Eyepieces: WF 10x / Ø 20 mm Objectives: 4x / PH10x / PH20x / PH40x / PH100x Product dimensions:... - Page 9 OBN-15-BA-e-2311...
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Page 10: Assembly
4 Assembly OBN-15-BA-e-2311... -
Page 11: Microscope Head
4.1 Microscope head First you must loosen the fixing screw on the tube connection point and remove the black protective cover. You can then insert the round dovetail bracket on the head into the round dovetail bracket on the housing and fix it with the fixing screw. When doing this, you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures. -
Page 12: Ph Condenser
4.5 PH condenser We recommend that you use the coarse adjustment knob to bring the specimen stage to its uppermost position. Use the focus dial of the condenser to move the condenser holder to the central position. In this way the condenser can be fitted at the right place in the condenser holder and fixed with the adjusting screw. -
Page 13: Pre-) Focussing
5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration). -
Page 14: Adjusting The Interpupillary Distance
5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly. -
Page 15: Adjusting The Magnification
5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path. -
Page 16: Adjusting The Koehler Illumination
5.6 Adjusting the Koehler illumination To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. If, as with the devices in the KERN OBN-15 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 17 3. Adjust the height of the condenser until the image from the field diaphragm appears clearly in the field of view. For some microscopes there is a risk that you will lift the condenser up so high that it collides with the object holder. Therefore care is needed when doing this.
- Page 18 6. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image. The scale divisions on the condenser can be used as a guideline. Select in accordance with the objective being used. The view in the tube without the eyepiece should look something like the illustration on the right.
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Page 19: Using Eye Cups
5.7 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces. -
Page 20: Using Oil Immersion Objectives
5.8 Using oil immersion objectives The 100x objectives of the OBN-15 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. 1. -
Page 21: Phase-Contrast Units
5.9 Phase-contrast units Comprehensive phase-contrast unit This consists of a PH universal rotary condenser, four PH objectives (10x, 20x, 40x and 100x), a centring telescope, two centring turnscrews and a green filter. This comprehensive PH unit is always included in the standard outfit of the models of the series OBN-15. - Page 22 The adjustment for this is carried out as follows. a. First adjust the microscope using bright field mode. b. Move the hub to the required PH position (e.g. “10x”). c. Open the aperture diaphragm on the condenser to its maximum (slider to the left).
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Page 23: Changing The Bulb
6 Changing the bulb Halogen (OBN 158) You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt. Before changing the bulb the device must be switched off and unplugged. -
Page 24: Changing The Fuse
After the lamp holder is folded back into its position and fastened, the change of the LED is complete. 7 Changing the fuse (OBN 159) The fuse housing is on the rear of the microscope below the mains power supply socket. -
Page 25: Using Optional Accessories
8 Using optional accessories 8.1 Camera connection Due to the trinocular tube, which is a standard fitting for the whole OBN-15 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed. After the plastic cover has been removed from the camera adapter connector on the top of the microscope head, then a suitable adapter must be fitted. -
Page 26: Trouble Shooting
9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set... - Page 27 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
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Page 28: Service
10 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN. - Page 29 NOTES __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ ________________________________________________________ OBN-15-BA-e-2311...
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