KERN Optics OBD 127 User Instructions

KERN Optics OBD 127 User Instructions

Transmitted light laboratory microscope digital
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User instructions
Transmitted light laboratory microscope
(digital)
KERN
OBD-1
Version 1.0
01/2015
OBD-1-BA-e-1510
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-Mail: info@kern-sohn.com
OBD 127, OBD 128
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com

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Summary of Contents for KERN Optics OBD 127

  • Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-Mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light laboratory microscope (digital) KERN OBD-1 OBD 127, OBD 128 Version 1.0 01/2015 OBD-1-BA-e-1510...
  • Page 3: Table Of Contents

    KERN OBD-1 Version 1.0 01/2015 User instructions Transmitted light microscope (digital) Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............
  • Page 4: Before Use

    1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments.
  • Page 5: Storage

    Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down.
  • Page 6: Maintenance And Cleaning

    1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth.
  • Page 7: Nomenclature

    2 Nomenclature OBD-1-BA-e-1510...
  • Page 8 Rear view OBD-1-BA-e-1510...
  • Page 9: Technical Data / Features

    3 Technical data / Features Model Standard configuration KERN Optical system Tube Illumination OBD 127 Infinity Trinocular 6V / 20W Halogen (Transmitting) OBD 128 Infinity Trinocular 6V / 20W Halogen (Transmitting) Eyepieces: WF 10x / Ø 20 mm Objectives: 4x / 10x / 40x / 100x...
  • Page 10 OBD-1-BA-e-1510...
  • Page 11: Assembly

    4 Assembly 4.1 Microscope head First you must loosen the fixing screw on the tube connection point and remove the black protective cover. You can then insert the round dovetail bracket on the head into the round dovetail bracket on the housing and fix it with the fixing screw. When doing this, you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures.
  • Page 12: Condenser

    4.5 Condenser We recommend that you use the course adjustment knob to bring the specimen stage to its uppermost position. Use the focus dial of the condenser to move the condenser holder to the central position. In this way the condenser can be fitted at the right place in the condenser holder and fixed with the adjusting screw.
  • Page 13: Pre-) Focussing

    5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).
  • Page 14: Adjusting The Interpupillary Distance

    5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly.
  • Page 15: Adjusting The Magnification

    5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path.
  • Page 16: Adjusting The Koehler Illumination

    5.6 Adjusting the Koehler illumination To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. If, as with the devices in the KERN OBD-1 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light.
  • Page 17 4. Open the field diaphragm until it just disappears out of the field of view. If necessary, simply re-centre using the centring screws on the condenser holder. 5. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image.
  • Page 18: Using The Camera

    Thus images or sequences of an object can be measured or digitally documented easily. Scope of delivery: USB cable Software disk Micrometre slide (for calibration) Specifications of camera + software: Image size OBD 127 3 MP Image size OBD 128 5 MP Sensor CMOS 1/2“...
  • Page 19: Using Eye Cups

    5.8 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces.
  • Page 20: Using Oil Immersion Objectives

    5.9 Using oil immersion objectives The 100x objectives of the OBD-1 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. 1.
  • Page 21: Changing The Bulb

    6 Changing the bulb You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt. Before changing the bulb the device must be switched off and unplugged. To change the bulb, tip the device carefully to the back or side.
  • Page 22: Changing The Fuse

    7 Changing the fuse The fuse housing is on the rear of the microscope below the mains power supply socket. With the device switched off and unplugged, you can pull out the housing. When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse can be removed from its housing and be replaced with a new one.
  • Page 23: Dark Field Units

    8.2 Dark field units There is the following way to carry out dark field applications. 1. A special dark field condenser can be used in place of the standard condenser. This as a paraboloid construction and also meets the requirements of professional application fields, in contrast to a dark field attachment.
  • Page 24 Simple phase-contrast unit This consists of a simple PH condenser, a PH objective with a specific magnification (10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a centring telescope and two green filters. To use this, you need to replace the standard condenser of the microscope with the PH condenser.
  • Page 25: Trouble Shooting

    9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set...
  • Page 26 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
  • Page 27: Service

    10 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.

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