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KERN Optics OBE-12 Operating Instructions Manual

Transmitted light laboratory microscope
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Transmitted light laboratory microscope
OBE 121, OBE 122, OBE 124, OBE 131, OBE 132, OBE 134
English version
Operating instructions Transmitted light laboratory microscope
Version
1.1
2024-09
en
OBE-12_13-BA-e-2411.docx
OBE-12, OBE-13
PROFESSIONAL MEASURING

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Summary of Contents for KERN Optics OBE-12

  • Page 1 Transmitted light laboratory microscope OBE-12, OBE-13 OBE 121, OBE 122, OBE 124, OBE 131, OBE 132, OBE 134 PROFESSIONAL MEASURING English version Operating instructions Transmitted light laboratory microscope Version 2024-09 OBE-12_13-BA-e-2411.docx...
  • Page 2 You can find other language Further language versions Más versiones de idiomas versions online at you will find online under se encuentran online bajo www.kern- www.kern- www.kern- sohn.com/manuals sohn.com/manuals sohn.com/manuals Inne wersje językowe znajdą Vous trouverez d'autres Trovate altre versioni di Państwo na stronie versions de langue online lingue online in...
  • Page 3: Table Of Contents

    English KERN Optics OBE-12, OBE-13 laboratory microscope Transmitted light Operating instructions Transmitted light laboratory microscope Version 2024-09 English version Table of contents: Technical data ........................... 3 Declaration of conformity ......................5 Overview of the device......................6 Nomenclature ..........................6 Before use ..........................9 General information ........................
  • Page 4 Troubleshooting ........................27 Service ............................. 30 Power supply .......................... 31 15.1 Mains connection ........................31 Maintenance, servicing and disposal ................... 32 16.1 Cleaning ........................... 32 16.2 Maintenance and repair ......................32 16.3 Waste disposal ......................... 33 Further information ........................ 34 2 | en OBE-12_13-BA-e-2411.docx...
  • Page 5: Technical Data

    Technical data OBE 121 OBE 122 OBE 124 Kern model Item number/type OBE 121 OBE 122 OBE 124 Dimensions (WxDxH) 360x395x320 mm Tubus Art Monocular Binocular Trinocular Optical system Finite Revolving nosepiece screw-in positions Lens quality Achromatic Standard objectives Eyepiece field width Illuminance Transmitted light Type of lighting...
  • Page 6 OBE 131 OBE 132 OBE 134 Kern model Item number/type OBE 131 OBE 132 OBE 134 Dimensions (WxDxH) 360x395x320 mm Tubus Art Monocular Binocular Trinocular Optical system Finite Revolving nosepiece screw-in positions Lens quality Achromatic Standard objetives 100x Eyepiece field width Illuminance Transmitted light Type of lighting...
  • Page 7: Declaration Of Conformity

    Declaration of conformity The current EC/EU Declaration of Conformity can be found online at: https://www.kern-sohn.com/shop/de/DOWNLOADS/ OBE-12_13-BA-e-2411.docx en | 5...
  • Page 8: Overview Of The Device

    Overview of the device Nomenclature 6 | en OBE-12_13-BA-e-2411.docx...
  • Page 9 Description Eyepieces Lens barrel Camera adapter connection Microscope head / Tube Setting wheel X - Y axis Object Coarse adjustment knob Fine adjustment knob Dimmer+ Main switch OBE-12_13-BA-e-2411.docx en | 7...
  • Page 10 Field lens Condenser Specimen stage Object holder Objective Nosepiece Power connection 8 | en OBE-12_13-BA-e-2411.docx...
  • Page 11: Before Use

    Before use General information The packaging must be opened carefully to prevent the accessories inside from falling to the floor and breaking. In general, a microscope should always be handled with great care, as it is a sensitive precision instrument. Avoiding abrupt movements during operation or transportation is therefore particularly important, especially to avoid endangering the optical components.
  • Page 12: Basic Information (General)

    Basic information (general) General information on warnings Warnings are used in these operating instructions to warn you of possible personal injury or damage to property in certain situations. Signal word Description DANGER Failure to observe the instructions will lead directly to serious injury, permanent impairment (e.g.
  • Page 13: Intended Use

    Indicates a prescribed action Intended use The OBE-12 and OBE-13 are versatile and are mainly used for the analysis of translucent and thin, high-contrast, less demanding specimens (e.g. plant tissue, stained cells and parasites). Improper use Do not use the device in potentially explosive atmospheres or for measurements in liquids or on live parts.
  • Page 14: Basic Warnings And Safety Instructions

    Basic warnings and safety instructions Observe the notes in the operating instructions Read the operating instructions carefully before commissioning/using the device, even if you already have experience with KERN devices. Always keep the instructions in the immediate vicinity of the appliance. Staff training The appliance may only be used by persons who have read and understood the operating instructions, in particular the chapter on safety.
  • Page 15 ⚠ WARNING Risk of injury due to electric shock! ● Risk of short circuit due to penetration of liquids into the housing! ● Do not immerse the appliance or accessories in water. Make sure that no water or other liquids get into the housing. ●...
  • Page 16 CAUTION Keep a sufficient distance from heat sources. Do not use the device in environments with high humidity or water mist NOTE ● To avoid damaging the device, do not expose it to extreme temperatures, extreme humidity or moisture. ● Do not use harsh cleaners, abrasive cleaners or solvents to clean the appliance. 14 | en OBE-12_13-BA-e-2411.docx...
  • Page 17: Transportation And Storage

    Transportation and storage Note If you store or transport the device improperly, the device may be damaged. Observe the information on transporting and storing the appliance. Transportation We recommend using the original packaging for shipping, transportation or storage of the microscope components. To prevent damage from shocks, all moving parts that can be assembled and disassembled must be packed separately.
  • Page 18: Unpacking And Commissioning

    Unpacking and commissioning Unpacking In the event of a return, please observe the instructions in the chapter "Packaging/return transportation On receipt of the device, you should first check that no damage has occurred during transportation, that the outer packaging, the housing, other parts or even the device itself have not been damaged.
  • Page 19: Assembly

    Assembly Microscope head The microscope head is firmly attached to the rest of the microscope and therefore cannot be removed or rotated. The tube, however, can be rotated 360° thanks to the butterfly design, which allows flexible adjustment of the eyepieces. Objectives Depending on the model, all three or four lenses are already screwed onto the nosepiece.
  • Page 20: Operation

    Operation 10.1 First steps The first thing to do is to connect the power supply using the mains plug. The light intensity control (dimmer) should first be set to a low level so that the eyes are not immediately exposed to too much light when looking into the eyepieces for the first time.
  • Page 21: Pre-) Focusing

    10.2 (Pre-) focusing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).
  • Page 22: Adjusting The Interpupillary Distance

    10.3 Adjusting the interpupillary distance (for binocular and trinocular devices) With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly.
  • Page 23: Adjusting The Magnification

    10.5 Adjusting the magnification After pre-focusing has been carried out using the lens with the lowest magnification (see 10.210.2),you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path.
  • Page 24: Setting The Lighting

    In order to obtain perfect image results during microscopic observation, it is important that the microscope's light guidance is optimized. The control element that plays the most important role in the OBE-12 and OBE-13 series is the height-adjustable condenser with aperture diaphragm.
  • Page 25: Using The Eyecups

    10.7 Using the eyecups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece and the result is a better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces.
  • Page 26: Use Of Oil Immersion Lenses

    10.8 Use of oil immersion lenses The 100x objectives of the OBE-13 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps.
  • Page 27: Changing The Bulb

    Changing the bulb The appliances in the OBE-12 and OBE-13 series with lighting are all equipped with LED lamps. Due to the long service life of LED lighting, regular lamp replacement will not be necessary with this microscope. In most cases, problems with the lighting would therefore be caused by defects in the electrical system.
  • Page 28: Use Of Optional Accessories

    Use of optional accessories 12.1 Dark field unit The following option is available for realizing dark field applications. A dark field insert with an integrated black disk can be screwed into the standard condenser of the microscope from below (see illustration on the right).
  • Page 29: Troubleshooting

    Troubleshooting Problem Possible causes The mains plug is not correctly plugged in The bulb does not light There is no power at the socket Defective bulb aperture diaphragm and/or field diaphragm are not opened wide enough The field of view is dark The condenser is not correctly centred brightness control...
  • Page 30 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
  • Page 31 When you move the table, the image The stage was not correctly fitted becomes blurred OBE-12_13-BA-e-2411.docx en | 29...
  • Page 32: Service

    Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.
  • Page 33: Power Supply

    Power supply 15.1 Mains connection The microscope may only be connected to the mains if the information on the microscope ( sticker ) and the local mains voltage are identical. Important: ▪ Check the mains cable for damage before commissioning ▪...
  • Page 34: Maintenance, Servicing And Disposal

    Maintenance, servicing and disposal Disconnect the appliance from the power supply before carrying out any maintenance, cleaning or repair work. 16.1 Cleaning The appliance must always be kept clean and regularly freed from dust. Before wiping the appliance when it gets wet, make sure that the power is switched off.
  • Page 35: Waste Disposal

    16.3 Waste disposal Old appliances and accessories must not be disposed of with household waste. The operator must dispose of the packaging and the device at the place of use in accordance with the applicable national or regional legislation The device consists of various components and materials, such as •...
  • Page 36: Further Information

    Further information The illustrations may differ slightly from the product. The descriptions and illustrations in these operating instructions are subject to change without notice. Further developments to the device may result in such changes. All language versions include a non-binding translation. The original German document is binding.

This manual is also suitable for:

Obe-13Obe 121Obe 122Obe 124Obe 131Obe 132 ... Show all