KERN Optics OBE 121 User Instructions
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KERN Optics OBE 121 User Instructions

Transmitted light laboratory microscope
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User instructions
Transmitted light laboratory microscope
KERN
OBE-1
Version 1.0
01/2020
OBE-12_13-BA-e-2020
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-Mail: info@kern-sohn.com
OBE 121, OBE 122, OBE 124
OBE 131, OBE 132, OBE 134
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com

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  • Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-Mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light laboratory microscope KERN OBE-1 OBE 121, OBE 122, OBE 124 OBE 131, OBE 132, OBE 134 Version 1.0 01/2020 OBE-12_13-BA-e-2020...

  • Page 3: Table Of Contents

    KERN OBE-1 Version 1.0 01/2020 User instructions Transmitted light microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............8 Assembly ..................

  • Page 4: Before Use

    1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments.

  • Page 5: Storage

    1.3 Storage You should ensure that the device is not exposed to direct sunlight, temperatures which are too high or too low, vibrations, dust or a high level of humidity. The ideal temperature range is between 0 and 40°C and a relative humidity of 85% should not be exceeded.

  • Page 6: Maintenance And Cleaning

    1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth.

  • Page 7: Nomenclature

    2 Nomenclature Lens barrel Camera adapter Eyepiece connection Microscope head / Tube Control dial X – Y axis Nosepiece Specimen Objective stage Object holder Coarse Specimen stage adjustment knob Condenser Fine adjustment knob Field lens Dimmer Main switch OBE-12_13-BA-e-2020...
  • Page 8 Power connection OBE-12_13-BA-e-2020...

  • Page 9: Technical Data / Features

    3 Technical data / Features Standard configuration Model Optical Tube Eye pieces Objectives Illumination KERN system Achromatic OBE 121 Finite Monocular WF 10x / Ø 18 mm 3W LED (Transmitted) 4x/10x/40x Achromatic OBE 122 Finite Binocular WF 10x / Ø 18 mm...
  • Page 10 OBE-12_13-BA-e-2020...

  • Page 11: Assembly

    4 Assembly 4.1 Microscope head The microscope head is firmly attached at the microscope body and cannot be removed. However thanks to the butterfly design the tube is rotatable by 360°, which enables a flexible adjustment of the eyepieces 4.2 Objectives Depending on the model all three or four objectives are already mounted to the nosepiece.

  • Page 12: Operation

    5 Operation 5.1 Getting started The very first step is to establish a power connection using the mains plug. After switching on the lighting you should first adjust the dimmer to a low level, so that when you look through the eyepiece for the first time, your eyes are not immediately subject to a high level of light.

  • Page 13: Pre-) Focussing

    5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).

  • Page 14: Adjusting The Interpupillary Distance

    5.3 Adjusting the interpupillary distance (For binocular and trinocular devices) With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly.

  • Page 15: Adjusting The Magnification

    5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path.

  • Page 16: Adjusting The Illumination

    5.6 Adjusting the illumination To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. The necessary control elements for this are the height-adjustable condenser with aperture diaphragm.

  • Page 17: Using Eye Cups

    5.7 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces.

  • Page 18: Using Oil Immersion Objectives

    5.8 Using oil immersion objectives The 100x objectives of the OBE-13 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. 1.

  • Page 19: Using Optional Accessories

    7 Using optional accessories 7.1 Dark field unit There is the following way to carry out dark field applications. 1. A dark field attachment with an integrated black disk can be screwed into the standard condenser of the microscope from below (see right illustration).

  • Page 20: Trouble Shooting

    8 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in The bulb does not light There is no power at the socket Defective bulb The aperture diaphragm and/or field diaphragm are not opened wide enough The field of view is dark The condenser is not correctly centred The brightness control has been set incorrectly...
  • Page 21 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...

  • Page 22: Service

    9 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.
  • Page 23 NOTES OBE-12_13-BA-e-2020...

This manual is also suitable for:

Obe 1 seriesObe 122Obe 124Obe 131Obe 132Obe 134

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