Shimadzu CoreFocus Shim-pack NovaCore C18-HB Instruction Manual

日本語取扱説明書はP.4以降をご参照ください。
■ Introduction
This is the instruction manual for the Shim-pack NovaCore C18-
HB. The Shim-pack NovaCore C18-HB is a core-shell column that
utilizes an organic silica hybrid . To ensure stable and long-term
use of the Shim-pack NovaCore C18-HB, which has excellent
column performance, please read this instruction manual
carefully and use it correctly.
■ Operating Precautions
•
Please check for any abnormalities in the appearance and
packaging box of the column.
• Verify that there are no errors in the name of the packing
material, particle size, column size, etc., by checking the
labels attached to the packaging box and the column.
• The inspection report includes the column lot number,
column serial number, and chromatographic test conditions.
Please keep the report for future reference.
• The number of theoretical plate stated in the inspection
report is for quality checks at the time of shipment and does
not indicate the performance (specifications) of the column.
■ Column Performance
The Shim-pack NovaCore C18-HB undergoes rigorous QC testing
to ensure quality and stability. The shipping solvent is
Acetonitrile / Water = 45 : 55 (v : v). Refer to the table below for
the specification of the Shim-pack NovaCore C18-HB columns.
Item
Functional Group
Pore Size
Surface Area
Carbon Content
Endcapping
Column hardware
Usable pH range
Common temperature
Maximum temperature
* The upper temperature limit is 60°C, but using it at conditions where the pH of
mobile phase exceeds 8 at 60°C will accelerate the degradation rate of the column.
High Performance Packed Column for HPLC
Shim-pack NovaCore C18-HB [Reversed phase column]
取 扱 説 明 書
INSTRUCTION MANUAL
Specification
Octadecylsilyl (ODS) group
10 nm
200 m /g
2
11%
〇
Stainless (SUS)
1-12
20 - 40°C
60°C*
NOTE
Near the pH limit of use, please use a mobile phase containing at
least 10% organic solvent. At the pH limit, column lifetime may be
shortened depending on conditions such as temperature and mobile
phase composition. Additionally, continuing to use the column at the
maximum temperature limit may also reduce its lifetime.
■ Column Installation
• The flow direction of the column is indicated by an arrow on
the column label (→). When installing the column, ensure
that the flow direction arrow matches the mobile phase flow
direction.
•
Use PEEK or SUS tubes with an inner diameter of 0.25 – 0.3
mm (UHPLC: 0.1 – 0.2 mm) and an outer diameter of 1.6 mm.
However, do not use PEEK tubes when working with
fluorinated organic solvents such as tetrahydrofuran (THF),
chloroform, concentrated sulfuric acid, concentrated nitric
acid, dichloroacetic acid,
dimethyl sulfoxide (DMSO), and hexafluoroisopropanol
(HFIP).
• The 1.7 μm particle size column produces higher
backpressure than the 5 μm or 2.6 μm particle size columns.
Please be aware of the maximum pressure of the HPLC
system and connect the appropriate tubes. Generally,
UHPLC systems that have a maximum pressure above 60
MPa are appropriate for 1.7 µm columns.
• Use the shortest possible tubes connection from the injector
to the column to minimize peak broadening.
• Use male nuts or fittings for connecting the column. Be
careful to avoid creating any unnecessary gaps during the
connection. For information on column connections, please
also refer to the system guide and the operating manual of
the LC system.
•
Male nuts or fittings can be obtained with the following
product names and part numbers (P/N).
Item Name P/N
Male Nut, PEEK 228-18565-84
Male Nut Fitting Kit 228-45717-01
UHPLC Fitting 2 S 228-56867-41
NOTE
Particulates or air in the system flow line may deteriorate the
column. Before connecting the column, be sure to filter all solvents
and flush the flow line up to the column with mobile phase.
ERAD-0001-9087A
acetone, dichloromethane,
Pressure
Remarks
Limit
5 pcs 20 MPa
2 pcs 35 MPa
1 pc 130 MPa