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Allsheng Feyond-A500 Operation Manual

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Operation Manual
V1.1
Feyond-A500
Microplate Reader
Hangzhou Allsheng Instruments Co.,Ltd.

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  • Page 1 Operation Manual V1.1 Feyond-A500 Microplate Reader Hangzhou Allsheng Instruments Co.,Ltd.
  • Page 2 Version modification Record: Version No. Date Modification Description V1.0 2024.04.03 Initial Release 1.Add handling instructions; 2. Add fuse V1.1 2024.06.25 information...

  • Page 3: Foreword

    If anything wrong or missing, please contact the distributor or the manufacturer. HANGZHOU ALLSHENG INSTRUMENTS CO., LTD. Add: Building 9 No.7 of Zhuantang Science and Technology Economic Zone, Xihu District, Hangzhou City, 310024 Zhejiang P.R. China Tel.: 0086-0571-88948289...

  • Page 4: Safety Warnings And Guidelines

    Safety Warnings and Guidelines 1. Important information for safe use Users should have a clear main idea on how to use this instrument before operate, please read this manual carefully prior to operation. Any improper operation may cause injured or electric shock. Please read the manual carefully and operate safely according to the guidelines.
  • Page 5 Warning: Avoid injury. Keep your body or any part of body away 15cm (or more) from the instrument when running. Except for the part which can be opened by the user according to the manual, the user is forbidden to disassemble the instrument. Doing so will disqualify your warranty and may result in an electric shock.

  • Page 6: Table Of Contents

    Contents Foreword......................I Safety Warnings and Guidelines ............... II Contents......................IV Chapter 1 Introduction...................1 Chapter 2 Features ..................3 Chapter 3 Instrument structure..............6 Chapter 4 Installation..................8 1． Opening check ..................8 2． Installation requirement..............8 3． Installation steps................. 8 Chapter 5 Operation Guide ................

  • Page 7: Chapter 1 Introduction

    Chapter 1 Introduction Chapter 1 Introduction Feyond-A500 is a powerful and mult-mode microplate reader. It is a widely used biological equipment, according to the principle of enzyme labeling, the presence, absence and depth of the color were analyzed qualitatively or quantitatively by enzyme-linked immunoassay.
  • Page 8 Feyond-A500 Microplate Reader Operation Manual Chapter 1 Introduction 7) The instrument mainly consists of four read types: EndPoint, Kinetic and Spectrum scanning, and well scanning functions. 8) With temperature control system which can be used to incubate the microplate, suitable for temperature sensitive experiments;...

  • Page 9: Chapter 2 Features

    Chapter 2 Features Feyond-A500 Microplate Reader Operation Manual Chapter 2 Features Working conditions: Ambient temperature: 10C~40C The relative humidity: 10%~80%(not be condensed) Power: AC100-240V 50-60Hz 2A The basic parameters and characteristics Name Microplate reader Model Feyond-A500 Absorbance(ABS), fluorescence(FL), Function luminescence(Glow, Flash), fluorescence polarization...
  • Page 10 Chapter 2 Features Feyond-A500 Microplate Reader Operation Manual Filter EX / EM 3 groups, single module insert and remove ≤2.5pM(Under optimized conditions, it can detect down Detection limit to 1 pM.) Linear dynamic range 6 logs Luminescence Plate Formats 6-, 12-, 24-, 48-, 96-, 384-well...
  • Page 11 Chapter 2 Features Feyond-A500 Microplate Reader Operation Manual ±1μL @ 5-50μL Accuracy ±2% @ 51-1000μL Waste liquid collection 50mL Others 2 USB A type ports 1 USB B type port Instrument port 1 Ethernet port Rs232 bus interface (injector connection) Size(W×D×H)mm...

  • Page 12: Chapter 3 Instrument Structure

    Feyond-A500 Microplate Reader Operation Manual Chapter 3 Instrument structure Chapter 3 Instrument structure This chapter mainly introduces the structure of the instrument. Before first use of this instrument, please read this chapter carefully to make a better preparation. Structure Upper cover...
  • Page 13 Feyond-A500 Microplate Reader Operation Manual Chapter 3 Instrument structure Air outlet Internet port RS232 bus interface USB port PC port Power socket Switch ─ ─...

  • Page 14: Chapter 4 Installation

    Chapter 4 Installation Chapter 4 Installation 1．Opening check Each Feyond-A500 is thoroughly tested before shipping, but please check again when you receive the instrument. Note if the following damage exist: The outer package is up side down or damaged The outer package has any obvious moisture stains...
  • Page 15 Feyond-A500 Microplate Reader Operation Manual Chapter 4 Installation Note: The instrument needs to be carried by multiple people. Open the side door and press the button on the head of the locking pin with your finger to release the locking. Keep the pressing state, and upward to pull out the locking pin.

  • Page 16: Chapter 5 Operation Guide

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Chapter 5 Operation Guide 1. Start the instrument After installing the instrument according to chapter 4, turn on the power switch to start the instrument and enter the self-checking interface as Fig 5-1.
  • Page 17 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5-1 lists the permissions of the three types of users. Table 5-1 Name Permissions content Remark User administrator permissions, all permissions related to Unique account, Admin user operations. Users cannot log in after forgetting the fixed name, initial password;...
  • Page 18 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 4 Change password interface After you select a user and enter the password, the system main interface is displayed, as Figure 5-5. Fig 5- 5 Main interface In this case, you can log out by pressing the button in the upper right corner, return to the user interface, and log in again.

  • Page 19: Protocol Settings

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide The files in this software are divided into three categories: protocol file (P), result file (R) and standard curve file (SC). Table 5-2 lists the file functions on the main interface;...
  • Page 20 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide After selecting protocol parameters, click the "OK" to enter the protocol interface, as Figure 5-7. It mainly consists of title bar, main display area, menu bar and option bar. Title bar...

  • Page 21: Title Bar

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5- 3 Relationship between read modes and read types EndPoint Kinetic Spectrum Well Scan 1.General 1.General 1.General 1.General 2.Shake 2.Detection 2.Shake 2.Shake 3.Advanced 3.Shake 3.Advanced 3.Advanced 4.Area Selection 4.Advanced 4.Area Selection 4.Area Selection...
  • Page 22 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5- 4 Functions of title bar Name Functions Clicking the pop-up prompt box asks the user if they want to save the current file. Clicking 'Yes' saves successfully. Clicking 'Cancel' returns to the current interface.
  • Page 23 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide all the wells in this column, and click the blank block above A to select all the wells of the plate. The microplate types range from 6 to 384 wells, and plates with fewer than 96 wells do not have scaling functionality.
  • Page 24 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 11 Heat diagram interface after protocol is finished Figure 5-12 shows the interface when the protocol is finished, when Kinetic or Spectrum is running. Fig 5- 12 Protocol finished interface(Kinetic) ─...
  • Page 25 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 2.3 Option bar In the options bar, switch between step and analysis at the top, and parameter display, set, and layout buttons at the bottom, as Figure 5-13. Fig 5- 13 Option bar 2.4 Menu bar...
  • Page 26 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Click to pop up the filter setting box; Click to execute shaking, lasting 1 second; When the plate of current instrument is out, click the plate into, otherwise the click plate out;...
  • Page 27 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 16 Injector interface 1 Fig 5- 17 Injector interface 2 Table 5-7 lists the functions of injection interface. Table 5- 7 Functions of injection interface Name Function Wash Select at least one injector to be washed;...

  • Page 28: Layout

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 5-18, showing the information about the current filter and the filter that can be replaced. Fig 5- 18 Filter interface Table 5-8 describes the functions of the filter pop-up box. Table 5- 8 Functions of filter interface...
  • Page 29 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Display area Option bar Sample type Fig 5- 19 Layout interface Different colors correspond to different options, and different options include different operational functions. Sample types mainly include blank, standard, quality control, unknown, negative, positive and clear. These seven types are in single mode and displayed with stroke display after selection, as Table 5-9.

  • Page 30: Step Parameter Setting

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Negative samples are used for classification of special Negative samples, default are replicates Positive samples are used for classification of special Positive samples, default are replicates This parameter is used to clear the well status and set the Clear well status to None.
  • Page 31 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Click "Read" to enter the Kinetic parameter setting interface. The Kinetic Settings are divided into total time and number of readings, as shown in Figure 5-21 and Figure 5-22 respectively. When choosing the total time, the calculation of reading number: Rounding the value of time/ interval and then adding 1.
  • Page 32 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5- 10 Function of Kinetic option columns Name Function Total Time/No. of Enable the total time to control the Kinetic cycle or according readings to the total number; Total time Enter the total kinetic cycle time, the maximum is 99:59:59;...
  • Page 33 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide advanced, and area selection parameters settings are the same as in endpoint method. The well scanning interface is shown in Figure 5-25. Fig 5- 24 ABS well scannin G factor interface...
  • Page 34 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide horizontal, vertical, cross, and fill, with the default being horizontal style. Taking a 12-well plate as an example, the well scanning settings are described in Table 5-11. Table 5- 11 Function of Kinetic option columns...
  • Page 35 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5- 12 Function of the general setting interface of Endpoint and Kinetic for FL Name Function Display the excitation wavelength EX and emission wavelength EM Wavelength of the instrument, which cannot be changed;...
  • Page 36 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 28 Liquid injection interface for LUM When select injector, as Figure 5-29 to 5-30. Input injector volume, default is 100μL. When both injectors are selected, the injector integrals are devided into injector volume 1 and injector volume 2, ranging from 5 to 500.
  • Page 37 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 31 The general setting interface of Endpoint and Kinetic for TRF Table 5- 13 Function of the general setting interface of Endpoint and Kinetic for TRF Name Function Display the excitation wavelength EX and emission wavelength EM Wavelength of the instrument, which cannot be changed;...
  • Page 38 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 32 FP Endpoint, Kinetics general settings interface Table 5- 14 Function for FP Endpoint, Kinetics general settings interface Name Function Display the excitation wavelength EX and emission wavelength EM Wavelength of the instrument, which cannot be changed;...
  • Page 39 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 33 G factor Settings Table 5- 15 Specific Functions of G factors Name Function Click to switch to the G factor setting interface. In the G factor G factor setting, you can select 3 options.
  • Page 40 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 34 Database Selection Choosing computation is depicted in Figure 5-35. Clicking on standard and blank wells prompts a well selection pop-up window, as shown in Figure 5-36. The default blank well is A1, and the default standard well is A2, with the default display area being a 96-well plate consisting of 8 rows (A-H) * 12 columns (1-12).
  • Page 41 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 35 Computation Fig 5- 36 Well selection ─ ─...
  • Page 42 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 37 Reminder for Unset Wells Table 5- 16 Function for computation Name Function When selecting multiple wells, the display shows the first well - the Standard well last well...
  • Page 43 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 4.9. Shake Click Shake to enter the shake interface, as shown in Figure 5-38. Fig 5- 38 Shake interface Table 5-17 lists the default shake settings. Table 5- 17 Default shake settings...

  • Page 44: Area Selection

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 4.10. Advanced Click the advanced button to enter the advanced setting interface. At this time, the sub-option is closed. The advanced screen is displayed, as shown in Figure 5-39. The pre-run wait times are all turned off by default. the default time for LUM is 00:01:00 with a range of 1min-30min, and the rest of the defaults are 00:00:25 with a range of 00:00:01-01:59:59.

  • Page 45: Analysis Interface

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 5. Analysis interface When the user clicks the "Analysis" button in the sidebar, the option bar will switch to the analysis interface, as Figure 5-41. The main display area includes the operation bar and the display area. The switch box is the data analysis area, and the option bar is the algorithm parameters and calculation type.

  • Page 46: Algorithm

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide corresponding content. For example, when a standard curve calculation method is selected in the data analysis area, the display area will be the standard curve interface, and the option bar on the right will be the parameter settings of the standard curve calculation method.
  • Page 47 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 1.Linear 1)The read values of the standard samples were linearly fitted by the least square method. 2.Linear(through origin) 1)The read values of the standard samples were linearly fitted by the least square method. And must through origin.
  • Page 48 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5- 21 Kinetic algorithms restrict relation Name Restriction condition Blank Subtraction Blank samples must be set Kinetic Analysis Kinetic readings must be performed The specific algorithm of the kinetic is shown in Table 5-22.
  • Page 49 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 1)Set the readings, select a single reading; 6.Select Reading Range 1)Set reading range and take the kinetic read data of each wavelength; 7.Maximum(Peak) 1)The maximum value of each curve 6.3 Spectrum Table 5-23 shows the constraints of the spectrum algorithm.

  • Page 50: Algorithm Parameter

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 1)Set the start and end wavelengths and read the measurements in the wavelength range. 5.Select Single Wavelength 1)Set the wavelength value and read the measured value at that wavelength. 6.4 Well scanning algorithm Well scanning algorithm is the same as the algorithm of the endpoint method, except that there is no qualitative analysis.
  • Page 51 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 42 Standard curve algorithm parameter interface Table 5-25 describes the parameters of the standard curve algorithm. Table 5- 25 Algorithm parameter function of standard curve Name Function Click to display the type selection interface of standard Fit type curve, as shown in Figure 5-43;...
  • Page 52 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide cubic spline, and Logit/log. Fig 5- 43 Selecting interface of standard curve type Fig 5- 44 Data Source interface Table 5-26 describes the functions of display area on the standard curve interface.
  • Page 53 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Saved to SC Click saved the standard curve to SC QR code Click to generate the standard curve to QR code Click to zoom to restore the initial value Click to switch to the run log interface...
  • Page 54 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide the data are displayed on the right. The selection type is single. Fig 5- 46 Data selection interface of basic calculation 7.3 Quality control When quality control is selected, the calculation parameter setting interface is shown in Figure 5-47.
  • Page 55 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 5-27 describes the parameters of the quality control calculation. Table 5- 27 Function of calculation parameters of quality control Name Function Click to enter the target value, default 1.000, range 0-99.999, up Target to three decimal places;...
  • Page 56 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Click to enter K1 values, default 1, range 0-99.999, Up to three decimal places; Click to enter K2 values, default 0, range 0-99.999, Up to three decimal places; Click to enter K3 values, default 0, range 0-99.999, Up to three decimal places;...
  • Page 57 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 7.6 Spectral analysis When spectral analysis is selected, calculation parameter settings vary according to the selection type. Figure 5-50 shows the selection interface of spectral analysis calculation type, which includes five calculation types, namely spectral maximum, spectral normalization, ratio within spectrum, select wavelength range, and select single wavelength.
  • Page 58 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 51 Algorithm parameter settings interface for Polarization 7.8 Anisotropy When the calculation method is set to Anisotropy, the algorithm parameter settings interface is shown in Figure 5-52. The original parameters set before running cannot be changed;...

  • Page 59: U-Nano Plate(Optional)

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 52 Algorithm parameter settings interface for Anisotropy 8. u-Nano Plate(optional) This microplate is an optional part. If the user has purchased the U-Nano plate of our company, this function can be used for U-Nano plate related tests. Click the "u-Nano"...
  • Page 60 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 53 Reading type of u-Nano 8.1 Nucleic acid Select nucleic acid on the u-Nano interface and click OK to enter the nucleic acid reading interface and create a protocol. The main display area of nucleic acid interface is shown in Figure 5-54 and 5-55.
  • Page 61 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 55 Nucleic acid interface(spectrum) Table 5-29 describes the function of nucleic acid interface. Table 5- 29 Function of nucleic acid interface Name Function Click to prompt whether to exit, if click "Yes" to prompt whether to save the current file, then click "Yes"...
  • Page 62 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide The reading mode can be selected from "multi-wavelength (230,260,280)" λ(nm) or "spectrum (220-350nm)"; Click to view the graph of the selected well, only when the reading mode is spectrum, the button will be available;...
  • Page 63 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 56 Curve interface The functions of the control area are shown in Table 5-31. The functions of the control area of protein and UV-VIS are consistent. Table 5- 31 Functions of the control area...
  • Page 64 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 57 File interface Table 5- 32 Function of file interface Name Function Search based on input Select the protocol to operate Displays the file name and the creation date...
  • Page 65 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Click to export the currently selected protocol to the USB disk, where the directory is U disk: \ Feyond\ username\ Nucleic, as shown in Figure 5-49. If there is no USB disk, it will prompt. The folder name of Protein is Protein, the folder name of UV-VIS is Uv, and the folder name of Cuvette is Cuv;...
  • Page 66 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide 8.2 Protein Select the protein in the u-Nano interface and click OK to enter the protein reading interface, as shown in Fig 5-60 and Fig 5-61, where protein reading can be performed.
  • Page 67 Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide coefficient of A280 is 10.0, BSA is 6.7, IgG is 13.7, Lysozyme is 26.4, and the default value of Others is 25.0. It can manually enter, the range is 0.01-99.99, as shown in Figure 5-62.

  • Page 68: Report Export

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 63 Uv-Vis interface 9. Report export After the results are processed, the processed data and raw data can be exported by the Report interface. Click "protocol" in the upper left corner, and then click "Report"...

  • Page 69: Power Off

    Feyond-A500 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5- 64 Report export In the File Type column, it can select the file format to export. Currently, two text formats are available:  Xls  Csv On the right of Export content, select the content to be exported by click. If √ is displayed on the right, the option is selected.

  • Page 70: Chapter 6 Maintenance, Storage, Transportation

    Feyond-A500 Microplate Reader Operation Manual Chapter 6 Maintenance, storage, transportation Chapter 6 Maintenance, storage, transportation 1. Maintenance  Keep storage environment dry and clean to prevent moisture, corrosion, away from strong electromagnetic interference sources.  Instrument already calibrated before leave factory. User is not allowed to disassembly and make adjustment.

  • Page 71: Chapter 7 Trouble Shooting

    Feyond-A500 Microplate Reader Operation Manual Chapter 7 Trouble shooting Chapter 7 Trouble shooting Failure and solution: Possible Trouble description Solution reason a.Check the if the instrument energized. b. If the power plug loose The Microplate Reader can Power supply c. Check the voltage...

  • Page 72: Memo

    Memo ─ ─...

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