Allsheng Fluo-200 Operation Manual

Portable fluorometer

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Operation Manual
V1.1
Fluo-200
Portable Fluorometer
Hangzhou Allsheng Instruments Co., Ltd.

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Summary of Contents for Allsheng Fluo-200

  • Page 1 Operation Manual V1.1 Fluo-200 Portable Fluorometer Hangzhou Allsheng Instruments Co., Ltd.
  • Page 2 Version modification Record: Version No. Date Modification Description V1.0 2022.01.11  Initial Release V1.1 2022.04.01  Interface upgrade...
  • Page 3 If anything wrong or missing, please contact the distributor or the manufacturer. HANGZHOU ALLSHENG INSTRUMENTS CO., LTD. Add: Building 1 & 2, Zheheng Science Park, Zhuantang Town, Xihu District, Hangzhou, Zhejiang 310024, China Tel.: 0086-0571-89948289...
  • Page 4 Safety and Operating Precautions 1. Safety Information Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation. NOTICE: Operation before reading Manual forbidden,otherwise instrument in operating may cause injury or electric shock.
  • Page 5 The Instrument should be put in dry place, less dust, far away from water and high-intensity light. What’s more, the place should be well ventilated, no corrosive gas or strong magnetic interference, far away from heater, stove or any other heat. Power off when you finish your work.
  • Page 6 Warranty Warranty content One month from the date of delivery, in case of faults due to material and manufacturing defects, our company will be responsible for replacement. Twelve months from the date of delivery, provide warranty for faults due to material and manufacturing defects.
  • Page 7: Table Of Contents

    Contents Chapter 1 Introduction ................1 Chapter 2 Specifications ................2 2.1 Basic parameters ................2 2.2 Performance parameter ..............2 Chapter 3 Instrument Structure ..............3 Chapter 4 Installation ................4 4.1 Opening Check ..................4 4.2 Installation Conditions ..............4 4.3 Installation Steps ................4 4.4 Operating Considerations ..............
  • Page 8: Chapter 1 Introduction

    (enzyme, receptosome, antibody), nucleic acid, hormone (steroid, thyroid hormones), medicine and microorganism. Fluo-200 fluorometer is based on fluoroimmunoassay to detect the luminous intensity of fluorescent reagent in immunoassay (IA). Under the condition of low concentration, the sample concentration is linear to fluorescence intensity.
  • Page 9: Chapter 2 Specifications

    Fluo-200 Portable Fluorometer Operation Manual Chapter 2 Specifications Chapter 2 Specifications 2.1 Basic parameters Parameters Specifications Ambient temperature 10C40C Relative humidity ≤70% Input 24VDC 2A Display 7 inch touch screen Dimension(W×D×H) 161mm×287mm×61mm N.W.(kg) 1.6kg 2.2 Performance parameter Parameters Specifications Dynamic range...
  • Page 10: Chapter 3 Instrument Structure

    Fluo-200 Portable Fluorometer Operation Manual Chapter 3 Instrument Structure Chapter 3 Instrument Structure This chapter introduces the structure of instrument. If it is used for the first time, please read this chapter carefully to make a better preparation. Structure 1:...
  • Page 11: Chapter 4 Installation

    Chapter 4 Installation Chapter 4 Installation 4.1 Opening Check Each Fluo-200 has been inspected strictly before packing and transportation. Please check again when you receive it. Contact with your local distributor or manufacturer in case of:  The package inverted or deformated;...
  • Page 12 Fluo-200 Portable Fluorometer Operation Manual Chapter 4 Installation Fluorescence PCR tube Fig 4. 1 Instrument operation diagram...
  • Page 13: Chapter 5 Software Introduction

    Chapter 5 Software Introduction 5.1 Self checking This chapter mainly introduces fluo-200 program operation. After connect power supply, turn on the switch on the back of instrument. The instrument power on and enter into self checking interface, then into Login interface.
  • Page 14: Dsdna Test

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction : User choice : Reagent calculation : dsDNA test : RNA test : Oligo test : Protein test : Fluorescence test : Standard curve : Report manage : Settings 5.3 dsDNA test...
  • Page 15 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction : Select different unit: ng/μL, ng/mL, μg/μL, μg/mL, mg/mL; : Click to check calibration parameters; : Click to print all current protocol test data; : Click to test current protocol; : Click to modify the protocol name;...
  • Page 16 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction Enter into calibration interface, insert information of standard sample 1 and then sample 2, click read to calibrate, then finish calibration. 5.3.3 Test After calibration, input volume of the sample, click , start to test, it will display original concentration and RFU after testing;...
  • Page 17 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction interface. Fig 5. 12 Calculator selection box Select the conversion unit, range: ng/μL, ng/mL, μg/μL, μg/mL, mg/mL; After conversion, the unit selection range is M, mM, μM, nM, pM. Input Molecular weight, DsDNA is 660g/mol by default and can be changed manually.
  • Page 18 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5. 13 Normalization calculate interface Fig 5. 14 Result interface The normalization result interface can be switched by clicking the button at the top TAB. The first interface shows the sample volume and buffer liquid volume to be added.
  • Page 19: Fluorescence

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction 5.4 Fluorescence In main interface, click , the options box is displayed, showing three test types: Blue 470nm, Red 635nm, Blue&Red. Fig 5. 17 Fluorescence test type Fig 5. 18 Blue 470nm test Fig 5.
  • Page 20: Standard Curve

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction parameters will automatically calculate the required dye and buffer liquid volume. Test range calculator: By selecting test type, measurement unit, and original sample volume, the range of test can be calculated automatically.
  • Page 21 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction : Filtered against item, such as dsDNA, RNA, Oligo, Protein; : New standard curve; : Manage standard curve; : Filtered against name; : Filtered against type; : Filtered against creator. 5.6.1 New standard curve Click new icon, The new curve category selection box is displayed, select item and test type, and input the name of curve;...
  • Page 22: Report

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction : Click to delete data of RFU1/RFU2/RFU3 or delete sample point; : Click to test fluorescence value; Note: For each standard sample, the average of fluorescence values from up to 3 tests can be used for curve fitting, less than 3 tests, no limit; For more than 3 tests, the top fluorescence value will be removed, and the latest test will be shown at the bottom.
  • Page 23: User Manage

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction the search box to quickly find the test report. Fig 5. 27 Report interface Fig 5. 28 Quickly search Click report to check tested data, click the sample again to view the detailed test data.
  • Page 24: Settings

    Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction : User switch, Click to enter login interface, and can switch user. 5.9 Settings Click in the main interface, enter settings interface; Fig 5. 32 Settings interface Function of buttons: system settings...
  • Page 25 Fluo-200 Portable Fluorometer Operation Manual Chapter 5 Software Introduction Click to enter system settings: Language: Select Chinese or English; Date and time: Set system date and time; Screen brightness: Adjust screen brightness; Sleep mode: Turn on or off sleep mode, set sleep time;...
  • Page 26: Chapter 6 Maintenance, Storage &Transportation

    Fluo-200 Portable Fluorometer Operation Manual Chapter 6 Maintenance, Storage and Transportation Chapter 6 Maintenance, Storage &Transportation 6.1 Maintenance  Keep storage environment dry and clean to prevent from moisture, corrosion, strong electromagnetic interference sources.  Instrument already calibrated before delivery. User is not allowed to disassemble the instrument.
  • Page 27: Chapter 7 Fault Analysis And Treatment

    Fluo-200 Portable Fluorometer Operation Manual Chapter 7 Fault Analysis and Treatment Chapter 7 Fault Analysis and Treatment Fault Analysis and Solutions: Fault Possible Causes Solutions phenomenon Instrument can Check the power supply; Power defective not start Check whether the plug is loose Check the power;...
  • Page 28 Memo...

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