Allsheng Auto-Pure Series Operation Manual
Nucleic acid purification system
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Summary of Contents for Allsheng Auto-Pure Series
- Page 1 Operation Manual V3.0 Auto-Pure Series Nucleic Acid Purification System Hangzhou Allsheng Instruments Co., Ltd.
- Page 2 Forewords Thank you for purchasing Auto-Pure series Nucleic Acid Purification System. In order to use the instrument properly, please read carefully this manual before operating and keep it for future reference. Opening Check Please check the instrument and Appendix with the packing list when you first open the package.
- Page 3 Safety Warnings and Guidelines 1 Warning Please read this Manual carefully before operation. Operation without reading the manual may cause damage or even electrical shock. 2 Safety Tips The operation, maintenance and repair of the Instrument should comply with the basic guidelines and cautions as below. Improper use of the instrument may cause damage to the system, inaccurate results, or potentially nullify warranties.
- Page 4 Before connecting to power, make sure the voltage used is same as the instrument required, and the maximum rated load should be sufficient for the instrument. Please replace the power cord with same specs if the power cord is damaged. Please make sure there’s nothing covered the power cord and keep it away from crowds when in use.
- Page 5 3 The maintenance of Instrument The inner side of drawer should be cleaned periodically by the cloth with alcohol. If there are any stains on the Instrument, clean them with cleansing cream. 4 The limiting condition of transportation and storage environment Ambient temperature range: 10℃～35℃...
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Page 6: Table Of Contents
Contents Chapter 1 Brief Introduction................1 Chapter 2 Specification..................2 1. Normal operating conditions................2 2. Basic parameter & Performance..............2 3. Overall Dimension...................3 Chapter 3 Product Introduction................. 4 1. Structure......................4 2. Operation panel....................6 Chapter 4 Operation................... 7 1. Preparation..................... 7 2. -
Page 7: Chapter 1 Brief Introduction
Chapter 1 Brief Introduction Chapter 1 Brief Introduction Auto-Pure series Nucleic Acid Purification System use the magnetic rod to adsorb, transfer and release magnetic particles to transfer the operating sample and in this way to purify DNA/RNA, protein and cell etc. The... -
Page 8: Chapter 2 Specification
Auto-Pure series Operation Manual Chapter 2 Specification Chapter 2 Specification 1. Normal operating conditions Environmental Temperature: 10C～35C Relative Humidity ≤70% Input: AC 100～240V，50Hz/60Hz 2. Basic parameter & Performance Table 1 Basic parameter & Performance Auto-Pure Auto-Pure Auto-Pure Auto-Pure Auto-Pure Auto-Pure... -
Page 9: Overall Dimension
Auto-Pure series Operation Manual Chapter 2 Specification Dimension 400mm×470mm×450mm 400mm×520mm×450mm (W×D×H) Weight（kg） 28kg 30kg 3. Overall Dimension Dimension（W×D×H） Auto-Pure 32A： 400mm×470mm×450mm Auto-Pure 48A： 400mm×470mm×450mm Auto-Pure 20A： 400mm×520mm×450mm Auto-Pure 20B： 400mm×520mm×450mm Auto-Pure 24D： 400mm×520mm×450mm Auto-Pure 24BT：400mm×520mm×450mm ─ ─... -
Page 10: Chapter 3 Product Introduction
Auto-Pure series Operation Manual Chapter 3 Product Introduction Chapter 3 Product Introduction This chapter basically introduce the instrument structure, operation buttons, display panel as well as the preparations before operate. For the first time user, please make sure to read this chapter before start. - Page 11 Auto-Pure series Operation Manual Chapter 3 Product Introduction Auto-Pure 20A/20B/24D/24BT Inside view 1.3. Magnetic rod (Over the magnetic rod UV light cover) (top inside) Magnetic rod cover stand Heating strip Drawer (bottom inside) Note: The only difference between the experiment module of above products is the shape of the heating strip.
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Page 12: Operation Panel
Auto-Pure series Operation Manual Chapter 3 Product Introduction 2. Operation panel Display screen STOP Display screen: Operate by touch screen or mouse which connect with USB port TAB: Select for the shortcut program RUN: Run for the shortcut program STOP: Stop operating ─... -
Page 13: Chapter 4 Operation
Auto-Pure series Operation Manual Chapter 4 Operation Chapter 4 Operation 1. Preparation Take out the instrument from packing carton and tear off the tape on the edge of operation window, then open it and take out the foam. First please screw out the fixed screw of position ①... -
Page 14: Connect The Power
Auto-Pure series Operation Manual Chapter 4 Operation 2. Connect the power Connect one end of power cord to the instrument socket and the other to power (AC100~240V), then turn on the power switch. 3. Install the reagent kit 3.1Install the customized reagent kit for Auto-Pure 20A & Auto-Pure 20B. - Page 15 Auto-Pure series Operation Manual Chapter 4 Operation Customized reagents kits Kits tray drawer door 3.3Install the 96 well reaction plate on Auto-Pure 32A. Take out the drawer and put the 96 well reaction plate which already filled with samples mounted on the locating slot, make sure the plate with chamfering should be on the left side, then push back the drawer slowly.
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Page 16: Install The Magnetic Rod Cover
Auto-Pure series Operation Manual Chapter 4 Operation 96 well reaction plate 4. Install the magnetic rod cover 4.1Install the magnetic rod cover for 20A/20B/24D/24BT Insert the magnetic rod cover completely on the mounting groove, and the installation quantity depends on the reagent kit number. -
Page 17: Remove Magnetic Rod Cover
Auto-Pure series Operation Manual Chapter 4 Operation 4.2Install the magnetic rod cover for Auto-Pure 32A/48A Insert the magnetic rod cover completely on the mounting groove, and the installation quantity depends on the reagent kit number. Auto-Pure 32A Magnetic rod cover Note: Magnetic rod cover for Auto-Pure32A at most is 4pcs, for Auto-Pure at most is 6pcs.The... -
Page 18: Operation
Auto-Pure series Operation Manual Chapter 4 Operation 6. Operation 6.1Start-up Interface Turn on the instrument and make sure the drawer is closed before start, the screen will display the start-up interface. After start, it will enter into the shortcut mode as below: ─... - Page 19 Auto-Pure series Operation Manual Chapter 4 Operation 6.2Program Run 6.3Shortcut mode Under the shortcut mode, select the program needed and click “Run”, it will enter into the program run interface. The program can also be selected by press “Tab” button on the panel and then press “Run” for start or “Stop”...
- Page 20 Auto-Pure series Operation Manual Chapter 4 Operation Auto-Pure 20B Running interface Auto-Pure 24BT Running interface ─ ─...
- Page 21 Auto-Pure series Operation Manual Chapter 4 Operation Auto-Pure 24D Running interface Auto-Pure 32A Running interface ─ ─...
- Page 22 Auto-Pure series Operation Manual Chapter 4 Operation Auto-Pure 48A Running interface On the left side, it shows the current step info., when the exact reagent position start to run, then this position will be highlighted, and there will be temperature display on the position of heating function.
- Page 23 Auto-Pure series Operation Manual Chapter 4 Operation 6.3.1 List mode Users may review the file by scroll bar on the right side. Select the program and click "Run" to enter into the run interface. Click “View” to enter into the check interface.
- Page 24 Auto-Pure series Operation Manual Chapter 4 Operation Click “Run” to enter the running interface. Click “Option” to view the parameter setting of the program. Click “Back” to previous interface. 6.3.2 Lamp At the bottom of interface, the icon “ ” shows that the lamp is on,and the icon “...
- Page 25 Auto-Pure series Operation Manual Chapter 4 Operation ─ ─...
- Page 26 Auto-Pure series Operation Manual Chapter 4 Operation Shortcut operation 6.4.1 Click “√” of the program in the “Manage Prog.” interface, the program will be displayed in the “Shortcut” list interface. In the list of “Lock”, if the icon is “...
- Page 27 Auto-Pure series Operation Manual Chapter 4 Operation Click “Insert” to add a new step. Click “Well” to insert the well number, then input the program name, waiting time, mixing time, magnetic time and sample volume. At the end, please click the mixing speed to select the speed.
- Page 28 Auto-Pure series Operation Manual Chapter 4 Operation For Auto-Pure 20A, only well location 1, 2 & 9 have heating function. For Auto-Pure 20B, only well location 1 & 7 have heating function. For Auto-Pure 24BT, only well location 1 & 8 have heating function.
- Page 29 Auto-Pure series Operation Manual Chapter 4 Operation Click “Cancel”, it will be back to “Management prog” interface and without save. Click “Cancel”, it will stay in the “Insert” surface. 6.4.3 Program Management--Edit Choose the program in the “Manage prog.” interface, then click “Edit” enter into edit program.
- Page 30 Auto-Pure series Operation Manual Chapter 4 Operation parameter of instrument. Remarks：Regularly, there’s no need to set unless it’s failed and need repair, as instrument has already finished setting before factory dispatch, and even if it’s failed, this kind setting will be authorized by distributor or manufacturer.
- Page 31 Auto-Pure series Operation Manual Chapter 4 Operation 6.5.3 System Setting--Language Click “Language Setting” to choose the language that you need. 6.5.4 System Setting -- Fan Click “ Air Ejector Fan” to have the fan setting ─ ─...
- Page 32 Auto-Pure series Operation Manual Chapter 4 Operation 6.5.5 System Setting -- Import/Export Click “Import/Export” and insert U disk to finish the step. 6.5.6 System Setting--Software upgrade Click “Software upgrade” to input the right password enter the interface and then insert the U disk to operation.
- Page 33 Auto-Pure series Operation Manual Chapter 4 Operation 6.5.7 UV sterilization Click “UV sterilization” and input number or click “+” “-”to set time. Click “Start” to open the UV light to start UV sterilization and time count down. Click “Stop” to stop the UV sterilization.
- Page 34 Auto-Pure series Operation Manual Chapter 4 Operation after the drawer is closed. 6.6 Help Click “Help” to check the help info.Help interface displays the relevant features and version information. ─ ─...
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Page 35: Chapter 5 Troubleshooting
Auto-Pure series Operation Manual Chapter 5 Trouble Shooting Chapter 5 Troubleshooting Fault phenomenon Possible Causes Solution Power not connected Check power Switch failure Replace switch No display after switch on Fuse failure Replace fuse （5X20 250V 8A） Others Contact with Distributor... - Page 36 Auto-Pure series Operation Manual Chapter 5 Trouble Shooting Software fault alarm list Fault type Fault name Error message Model T1,T2,T3,T4,T5,T6, E011,E021,E031,E041, T7,T8 Overheat E051,E061,E071,E081 T1,T2,T3,T4,T5,T6, E018,E028,E038,E048, T7,T8 Drive circuit fault E058,E068,E078,E088 Auto-Pure 32A Auto-Pure 48A T1,T2,T3,T4,T5,T6, E015,E025,E035,E045, T7,T8 Open circuit...
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Page 37: Chapter 6 Spare Parts List
Auto-Pure series Operation Manual Chapter 6 Spare Parts list Chapter 6 Spare Parts List 1. Auto-Pure 20A/20B Spare parts list Item Unit Remark Power line Mouse Kits tray Allen wrench 2. Auto-Pure 32A/48A /24D/24BT Spare parts list Item Unit Remark... -
Page 38: Chapter 7 Abbreviation And Symbols
Auto-Pure series Operation Manual Chapter 7 Abbreviation and Symbols Chapter 7 Abbreviation and Symbols 1. Abbreviation Abbreviation used Ampere Alternating current Volt Hertz Watt Universal Serial Bus Secure Digital Card WiFi WLAN Kilogram Millimeter μL Microlitre Hectopascal Degree Centigrade ℃... - Page 39 Auto-Pure series Operation Manual Chapter 7 Abbreviation and Symbols 2. Symbols Symbols used on device Warning Heating Indicates conformity with health, safety, and environmental protection standards for products sold within the European Economic Area ─ ─...
- Page 40 Auto-Pure series Operation Manual Notes Notes ─ ─...
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