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Harvard Bioscience biochrom Expert Plus Service Manual

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Expert Plus
Biochrom Ltd, Unit 7 Enterprise Park, 3970 Cambridge Research Park, Waterbeach, Cambridge, CB25 9PE, England
E-mail: enquiries@biochrom.co.uk
Service Manual
Microplate Reader
EZ read 800
Version: 5.1
Model
Expert Plus
Yes
EZ read 800 Plus
Yes
EZ read 800
No
Telephone: +44 (0)1223 423723
EZ Read 800 Plus
Display
Data connection
RS232
Mini USB
Mini USB
Fax: +44 (0)1223 420164
Support:
support@biochrom.co.uk

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  • Page 1 Service Manual Microplate Reader Expert Plus EZ read 800 EZ Read 800 Plus Version: 5.1 Model Display Data connection Expert Plus RS232 EZ read 800 Plus Mini USB EZ read 800 Mini USB Biochrom Ltd, Unit 7 Enterprise Park, 3970 Cambridge Research Park, Waterbeach, Cambridge, CB25 9PE, England Telephone: +44 (0)1223 423723 Fax: +44 (0)1223 420164 E-mail: enquiries@biochrom.co.uk...

  • Page 2: Table Of Contents

    Contents HEALTH AND SAFETY ARNINGS AND AZARDS ATED PERATING ONDITIONS LEANING AND ISINFECTING THE NSTRUMENT MERGENCY NSTRUCTIONS INTRODUCTION ENERAL ESCRIPTION ECHNICAL PECIFICATIONS 3.0 THEORY OF OPERATION NTRODUCTION AIN COMPONENTS PTICAL SYSTEM ROCESS ONTROL ALIBRATION RUN EASUREMENT LATE TRANSPORT SYSTEM / EZ 800 P EYBOARD AND ISPLAY...
  • Page 3 UNCTIONS OF THE ERVICE IAGNOSTIC 5.3.1 Service - Submenu “Transport” 5.3.1.1 Calibrate Transport 5.3.1.2 Position Test 5.3.2 Service - Submenu “Filter Wheel” 5.3.2.1 Calibrate Filter Wheel 5.3.2.2 Filter Wheel Position Test 5.3.3 Service - Submenu “Meas. unit” 5.3.3.1 Dioden Test 5.3.3.2 Filter Calibrate 5.3.3.3 Measurement 5.3.3.4 Define Plate Positions...
  • Page 4 7.4 R EPLACE AINS 7.5 R EPLACE OWER UPPLY 7.6 R EPLACE IGHT ONTROL OARD 7.7 R EPLACE 7.8 R EPLACE ENSORS 7.8.1 Transport Sensors Replacement 7.8.2 Filter Wheel Sensor Replacement 7.9 R EPLACE OARDS 7.9.1 Replace the CPU board 7.9.1.1 Data Backup 7.9.1.2 Physically replace CPU board 7.9.1.3 Restoring the original Data (if a backup as in 7.9.1.1 was possible)
  • Page 5 9.0 CLEANING AND DISINFECTION NTRODUCTION LEANING THE INSTRUMENT LEANING ILTERS NSTRUMENT ISINFECTION 9.4.1 Disinfection Procedure 11.0 PERFORMANCE VERIFICATION 12.0 PREVENTATIVE MAINTENANCE 13.0 SPARE PARTS LIST APPENDIX 1 - DECLARATION OF CONTAMINATION STATUS Expert Plus / EZ read 800 / EZ read 800 Plus Version: 5.1...

  • Page 6: Health And Safety

    1.0 Health and Safety Please note that only suitably qualified service engineers should open up and work on this equipment due to the hazards involved. 1.1 Warnings and Hazards There a number of warning labels and symbols on your instrument. These are there to inform you of a potential danger that may exist or where particular caution is required.

  • Page 7: Rated Operating Conditions

    Toxic Fumes. Efficient laboratory ventilation must be provided when working with volatile solvents or toxic substances. Waste disposal. Disposal of some solvents and chemicals may be classed as hazardous waste and must be dealt with in accordance with local regulatory practice. Personal protective equipment including but not limited to gloves, laboratory coats and safety glasses is recommended when using this instrument.

  • Page 8: Cleaning And Disinfecting The Instrument

    1.3 Cleaning and Disinfecting the Instrument For disinfection, authorized trained personnel must operate in a well-ventilated room while wearing disposable gloves, protective glasses and clothing. A declaration of decontamination form is given in Appendix 1 (at the end of this document). This should be completed and signed by the person who decontaminates the equipment.

  • Page 9: Introduction

    2.0 Introduction 2.1 General Description The EZ read 800 (Plus) and Expert Plus instruments are a microprocessor controlled 8-channel microplate reader designed to measure the optical density of liquids in the wells of 96-well microplates. It combines a large, graphics display with easy to use on-board software, making it ideal for use in any laboratory performing ELISA and other colorimetric microplate assays.

  • Page 10: Theory Of Operation

    3.0 Theory of Operation 3.1 Introduction This chapter gives the description of the main components, the optical system layout and description. 3.2 Main components The main components of the instrument are the CPU board, the pre-amplifier board with the photodiodes, the plate transport mechanism, the light source, the filter wheel and the power supply unit.

  • Page 11: Optical System

    3.3 Optical system The optical system consists of the lamp, the condenser lens, the interference filters on the six position filter wheel, the fibre bundle, a focusing lens for each of the eight measurement channels and eight silicon photodiodes with a focusing lens in front of each detector. 3.4 Process Control The CPU of the Expert Plus reader can control the lamp intensity by means of a 16-bit Digital-Analogue converter in combination with the lamp power-supply.

  • Page 12: Measurement

    3.6 Measurement After the initial calibration run, the required light intensity for each wavelength is “known” by the system, this means that for any subsequent measurements the light intensity is set to the stored value and only a check as described below is performed. The plate carrier moves partially inside the reader to avoid influence of external light.

  • Page 13: Plate Transport System

    3.7 Plate transport system The plate transport system consists of the plate carrier, a linear guide and a stepper motor driving the belt. The Plate Carrier itself features an auto centring function, so that any plate type can be inserted easily and is being brought into correct position as soon as the plate carrier moves the plate inside the instrument for reading.

  • Page 14: Keyboard And Display (Expert Plus/Ezread800 Plus Only)

    3.8 Keyboard and Display (Expert Plus/EZread800 Plus only) The display and the keyboard of the instrument represent the user interface and the components for the operator to communicate with the instrument. The Display is a graphical LCD display. The Keyboard gives access to all functions and menus of the instrument;...

  • Page 15: Instrument Setup Menu

    4.0 Instrument Setup Menu 4.1 Introduction This chapter gives the description of the Instrument Setup software menu. When the instrument is first switched on, or during standby, the display shows following main (home) screen: To enter the Instrument Setup Menu, press key “5”. The instrument now enters the Setup menu and displays the following screen: 4.2 Functions of the Setup Menu The possible settings in the Instrument Setup Menu are:...

  • Page 16: Printer Type/Model Selection

    4.2.1 Printer type/model selection The instrument has a range of printer drivers pre-programmed, these however are all for old printers that are no longer available. This setting is used to define which printer is connected to the instrument, so that the correct printer driver is being used.

  • Page 17: Setup Filters

    4.2.2 Setup Filters This menu point is used for entering / changing filter wavelengths when new / other filters are being inserted, and for calibrating filters. Calibrating the filters is required after first installation of the instrument. Also, when replacing one or more of the optical filters, the optical system must be calibrated to these (see more detailed explanation in chapter 3.5.1.2).

  • Page 18: Date / Time Setting

    4.2.3 Date / Time Setting Enter the Instrument Setup Menu as described in chapter 4.1. When the Instrument Setup Menu Start screen is displayed, select the menu point “Date/Time” by pressing the numerical key “3” and the display will show the following screen: Press “F1”...

  • Page 19: Language Setting

    4.2.5 Language Setting Enter the Instrument Setup Menu as described in chapter 4.1. When the Instrument Setup Menu Start screen is displayed, select the menu point “Language” by pressing the numerical key “5” and the display will show the following screen: Use the keys “Cursor Up”...

  • Page 20: Service / Diagnostic Menu

    5.0 Service / Diagnostic Menu 5.1 Introduction This instrument is fitted with a highly sophisticated service and diagnosis software for verification of instrument functions, trouble shooting and special settings. This chapter will explain the functions and the handling of this menu. 5.2 Enter the Instrument Service / Diagnostic Menu When the instrument is first switched on, or during standby, the display shows following screen: This menu allows settings that, if performed by un-authorised personnel or not performed...

  • Page 21: Service - Submenu "Transport

    5.3.1 Service - Submenu “Transport” This submenu allows to calibrate the plate transport and to check specific positions, and to move the plate transport “manually” step by step. Enter the Service/Diagnosis Menu as described in chapter 5.2. When the Service/Diagnosis menu start screen is displayed, select the menu point “Transport”...

  • Page 22: Service - Submenu "Filter Wheel

    This menu allows you to define specific positions, to move the plate carrier and to manually step the transport in and out. Use the keys “Cursor Up” and “Cursor Down” to select the entry fields for “Move to”. Enter values (between 0 and 3037) using the numerical keys.

  • Page 23: Filter Wheel Position Test

    5.3.2.2 Filter Wheel Position Test To enter the Position Test menu, press “3” from the filter wheel sub menu (5.3.2). The display will now show the following screen: This menu allows you to define specific positions to move the filter wheel to, and to check the Filter wheel sensor. Use the keys “Cursor Up”...

  • Page 24: Dioden Test

    5.3.3.1 Dioden Test This submenu can be considered as the main verification tool of the optical system of the instrument. To enter the “Dioden Test” menu, press “1” from the meas. unit submenu (5.3.3). The display will now show the following screen: This menu allows you to check each of the eight optical channels, with each of the available wavelengths and at selectable lamp intensity.

  • Page 25: Filter Calibrate

    Light distribution verification 1) Set Lamp intensity to 15000, use the keys “Cursor Up” and “Cursor Down” to apply the change in light to the lamp. The digital values of all channels must be in overflow (65535), with all filters. 2) Select Filter 1, increase lamp intensity step by step until the highest digital value is approx.

  • Page 26: Recall

    Use the keys “Cursor Up” and “Cursor Down” to select the entry fields. Use the keys “Cursor left” and “Cursor right” to select values. The values in “Bright” and “Dark” are factory set and should not be changed. The values in “1 Col”...

  • Page 27: Io Port

    5.3.4.1 IO Port This submenu is used to define some of the settings for communication between the instrument and connected printer. These settings should only be changed if there are problems with the printout and only after prior consultation of the manufacturer.

  • Page 28: Test

    5.3.4.4 Test This submenu is used to test the communication between the instrument and printer, and may be used for testing printer commands. The instrument has pre-defined test sets, which can be sent to the connected printer. To enter this submenu, press “4” from the printer submenu (5.3.4). The display will then show the following screen: Use the keys “Cursor Up”...

  • Page 29: Watch Sensors

    5.3.5.1 Watch Sensors This submenu is used to test the function of the optical sensors of the instrument. To enter this submenu, press “1” from the “others” submenu (5.3.5). The display will then show the following screen: This screen shows the actual status of all three sensors of the instrument. The instrument has three sensors: Transport Home, Transport End and Filter wheel.

  • Page 30: Test Keyboard

    ATTENTION: Setting LCD voltage to either Min or Max will have one of the following effects: LCD Min LCD Max Pressing the key “esc” will escape this submenu and get the display contrast back to the preset range (as defined in instruments settings, “Display Contrast”, chapter 4.2.4).

  • Page 31: Exit From Service Menu

    5.3.7 Exit from Service Menu To exit from Menus and submenus, press the “esc” key. Each time the “esc” key is pressed, the instrument will switch to the next upper level in the menu structure. If the “esc” key is pressed when the Service Menu Start screen is displayed, the instrument will perform an initialization routine and return to standby (home screen).

  • Page 32: Error Handling And Fault Finding

    6.0 Error handling and fault finding 6.1 Error indication If an error occurs during measurement, the corresponding error message or number is displayed in the status window of the PC measurement software or alternatively on the instrument display itself if being used in stand-alone mode. Error Codes for instrument errors: Instrument errors are indicated by a numeric and/or textual error message in the status window.
  • Page 33 Instrument Error Messages The following error messages may be displayed on the instrument screen when in stand-alone operation mode: Error Message Error Description Possible Remedy Filter wheel movement Check for wrongly inserted or Filter wheel movement timeout jammed. loose filter. Filter wheel movement Check for wrongly inserted or Filter wheel is too late in Low/High...

  • Page 34: Trouble-Shooting Error Codes

    6.2 Trouble-shooting Error Codes Error 1: General system error This error can happen during the initialisation of the reader after switch-on only. When one of the initialization procedures does not finish within a certain time, the error message no. 1 will be shown. Transport blocked, filterwheel blocked Components to be checked in case of Error 1: Plate transport, optical sensors, filter wheel, AD-converter.
  • Page 35 Error 7: In case the light intensity for the lowest and/or the highest channel measured before the actual reading is different from the light intensity found during the calibration, the error message 7 is shown. Possible causes and remedies: The lamp has changed the intensity. Perform a calibration and try some repeated measurements. If the error 7 disappears, monitor the instrument for the next days.
  • Page 36 6.3 Trouble-shooting Error Messages 6.3.1 General error This error may happen during the initialisation of the reader after switch-on. When one of the initialisation procedures cannot be finished within a certain time, error “1” will be shown. Components to be checked in case of Error 1: Plate transport, optical sensors, filter wheel, AD-converter.
  • Page 37 The filter wheel is not in the correct position. → Open the lid for the filter compartment and start a measurement. Watch if the light beam is in the centre of the filter. If this is not the case, the position of the filter wheel must be readjusted: Open the filter compartment at the right side of the instrument.

  • Page 38: Parts Replacement

    7.0 Parts Replacement 7.1 Introduction This chapter will give instructions on how to replace parts and components of the instrument and how to perform adjustments that might be necessary after replacing any parts. Some parts and components of the instrument can be replaced with the cover closed. Most parts however, require at least a partial disassembly of the instrument.

  • Page 39: Bottom Plate Removal

    4. Carefully lift the cover up and place to the left. The cover is now connected to the instrument by the display and keyboard cables. To completely remove the cover, disconnect the two ribbon cables from the instrument. To re-assemble the cover, follow the above description in reverse order. Do not tighten screws until they have all been located in the corresponding threaded holes.

  • Page 40: Replace Filters

    7.3 Replace Filters The instrument can accommodate up to 6 interference filters on a filter wheel. The filter wheel can be accessed by opening the filter compartment door, located on the right side of the instrument: The filters are held in place on the filter wheel by spring wires and can easily be replaced without using tools. Filter Position 1 Filter...

  • Page 41: Replace Mains Fuse

    7.4 Replace Mains Fuse The following steps must be performed to replace the mains fuse, which is in mains receptacle block, in the rear panel of the instrument. Fuse Cover Switch off the instrument and unplug the power cord. Open the plastic cover of the fuse compartment, by inserting a small screw driver into the slot at the right of the cover and pushing the cover out.

  • Page 42: Replace Light Control Board Fuse

    7.6 Replace Light Control Board Fuse This fuse protects the 24V supply used for the lamp and the stepper motors and is located on the light regulation board. Before this fuse can be accessed, the instrument cover, base plate and bottom plate must be dis-assembled as described in Chapters 7.2.1.

  • Page 43: Replace Sensors

    Note that the bulb used in the old UV variant differs to the Notch standard reader. The filament is much smaller in the UV version. Insert the new lamp, making sure that the spring loaded ball snaps into the notch of the lamp socket. Re-assemble the instrument in reverse order.

  • Page 44: Filter Wheel Sensor Replacement

    7.8.2 Filter Wheel Sensor Replacement The filter wheel sensor shares the connector of the filter wheel motor. The sensor is supplied with the filter wheel motor assembly; therefore the entire assembly must be replaced if a new sensor is required. Filter wheel connector Filter...

  • Page 45: Replace Boards

    7.9 Replace Boards WARNING: Always ensure you use ESD (Electro-Static Discharge) equipment whilst handling PCBs. An ESD wrist band and work mat are highly recommended. The instrument is fitted with five boards as shown below. Board Light Keyboard Control Interface connector Board Board...

  • Page 46: Replace The Cpu Board

    7.9.1 Replace the CPU board All plate data, methods and important instrument setup data are stored in the memory components of the CPU board. Therefore it is important to save these data before a CPU board has to be changed. This is only possible when the CPU board is at least partially functioning.

  • Page 47: Restoring The Original Data (If A Backup As In 7.9.1.1 Was Possible)

    7.9.1.3 Restoring the original Data (if a backup as in 7.9.1.1 was possible) Connect the reader to the computer, do not switch the reader on Copy VrRestore.exe from the service CD to your chosen directory. Copy vrdata.s3 and vrprog.s3 from the directory where you stored VrBackUp.exe to the directory where VrRestore.exe resides.

  • Page 48: Replace The Power Supply Board

    7.9.3 Replace the Power Supply board Before the Power Supply board can be accessed, the instrument cover, base plate and bottom plate must be dis-assembled as described in Chapters 7.2.1 and 7.2.2. ATTENTION: Before touching the Power Supply Board, make sure that the power cord is disconnected from the instrument! The Power Supply board is located in the bottom plate.

  • Page 49: Replace The Pre-Amplifier Board

    Arrow down to highlight ‘lamp’. Enter a value of 15500, followed by the up key that will highlight the box above and apply the changes to the lamp. With the lamp at this brightness, you must quickly turn the filter wheel to cover the optics and prevent damage to the fibre optics.

  • Page 50: Replace Lcd Display

    7.10 Replace LCD display The display is replaced complete with its cable. Before the Display board can be replaced, the instrument cover must be removed as described in Chapter 7.2.1. The display is mounted to the cover with 4 hex screws. After replacing the display, adjust the contrast as described in chapter 4.2.4 ‘Display Contrast setting’.

  • Page 51: Cpu Board Battery Replacement

    7.12 CPU Board Battery Replacement If the date/time or method settings reset themselves between switch on and off, then the battery has lost its charge and requires replacement. The factory standard battery is a 3V CR2430 battery, although higher capacity batteries can be obtained if required.

  • Page 52: Adjustments

    8.0 Adjustments Several adjustments are required after certain parts replacement. Use the sections below if instructed to in previous chapters or by the manufacturer. Important: Adjustments specified within this section have a major role in terms of functionality in accordance to the specification. Do not edit any parameter unless instructed to.

  • Page 53: Set Serial Number

    Tracing Paper Make adjustment to the position of the plate carrier by loosening the three 2.5mm hex bolts as shown in red circles above. Ensure that the holes in row 12 are correct after correct adjustment for the holes in row 1. The light beam must be perfectly central in relation to the holes in the Asys QC test plate.

  • Page 54: Light Intensity Voltage Adjustment

    8.3 Light Intensity Voltage Adjustment If you suspect an issue with the light control board or you need to verify the light control board functionality, these steps will ensure correct voltage. The Light intensity board is fitted with one potentiometer, which defines the range for the light intensity and which is factory adjusted.

  • Page 55: Darkness Value Adjustment

    8.4 Darkness Value Adjustment The darkness values are the values of each photodiode when they are receiving no light. The instrument top cover must be on during this check. Connect the reader to a PC with Mikrowin 2000 Open Mikrowin and select Installation > Driver. From the list of installed reader drivers, double click on ‘Asys HiTech Expert Plus’...

  • Page 56: Condenser Lens Adjustment

    8.5 Condenser Lens Adjustment If the light absorbed by the diode boards does not reach the maximum tolerance (62000) then adjustment to the condenser lens may be necessary. Tools required: 2mm Hex Driver 2.5mm Hex Driver Remove the six screws holding the upper housing of the instrument to the base assembly. With the instrument door facing you, lift the cover off towards the left side and place next to the instrument.

  • Page 57: Light Distribution

    8.7 Light Distribution If you are experiencing issues with the optical system, it is necessary to check the light output from each of the 8 channels. This guide should also be followed if new fibre optics are fitted. Enter the instrument service / diagnostic menu. When the instrument is first switched on, or during standby, the display shows the following screen: This menu allows settings that, if performed by un-authorised personnel or not performed properly, may cause major malfunctions of the instrument!
  • Page 58 This menu allows you to check each of the eight optical channels, with each of the available wavelengths and at selectable lamp intensity This screen allows you to verify the functionality of the: Actual digital values of channel A (now selected in this example). Actual digital value of channels Meaning of the three numbers: A –...
  • Page 59 Fine adjustment of the condenser lens position. Adjustment of the lamp position. By polishing the ferrule end of the optics on fibres with low light levels (a cotton swab is good for polishing) Fine adjustment of the fibre optic loom, it is possible to turn the ferrules slightly. Do not turn them too much or they will break! If new fibre optics are fitted or old removed for adjustment Once all values are within tolerance, apply two-part epoxy resin glue to the ferrules on both ends of the optics...

  • Page 60: Cleaning And Disinfection

    9.0 Cleaning and Disinfection 9.1 Introduction This chapter gives instructions on how to clean parts, and how to properly maintain the instrument in order to assure longest possible functionality. Also the procedure for disinfection of the instrument is described. 9.2 Cleaning the instrument This instrument is a precision instrument and requires regular cleaning to ensure the continued precision.

  • Page 61: Performance Verification

    11.0 Performance Verification QC Plate (P/N: SG010160): To check the performance of the Instrument, we recommend the use of an Asys QC plate with WinQC software. The QC plate checks the mechanical alignment of the plate transport, the optical path and tests the accuracy and precision. The QC plate consists of a precisely machined aluminium body in the dimensions of a standard microplate and three neutral density glass filters.

  • Page 62: Preventative Maintenance

    12.0 Preventative Maintenance The use of the following checklist is at the discretion of the service engineer. It is intended to help service engineers conduct servicing in a consistent and methodical manner. Copies of this check list are not formally required for instrument user or Biochrom Ltd. This section can however be printed, filled out and kept as a record of the service history.

  • Page 63: Spare Parts List

    13.0 Spare Parts list Description Part Number Orange Test Plate S5450174 Dust cover (for instruments without a display) 80-4002-61 Dust cover (for instruments with a display) 80-4002-62 Stepper Motor (X Transport) 80-4000-31 Motor Gear Wheel (Pack of 3) S5480010000 Cog (Rear - X Transport) S5480009 Plate Transport Sensors &...
  • Page 64 Description Part Number 500nm Filter SB010080 504nm Filter SB010074 510nm Filter SB010079 517nm Filter SB010085 520nm Filter SB010082 525nm Filter SB010081 535nm Filter SB010297 540nm Filter SB010086 546nm Filter SB010105 550nm Filter SB010063 560nm Filter SB010084 562nm Filter SB010096 570nm Filter SB010067 580nm Filter SB010094...

  • Page 65: Appendix 1 - Declaration Of Contamination Status

    Appendix 1 - DECLARATION OF CONTAMINATION STATUS Prior to the Inspection, Servicing, Repair or Return of Medical and Laboratory Equipment. Customer: Address: Tel No Fax No: Instrument Type Serial Number: Complete section A / C and if applicable complete all of section B, providing further information as requested or appropriate. A.

This manual is also suitable for:

Biochrom ez read 800Biochrom ez read 800 plus

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