40 vials of 2 mL each. When equipped with the quadruple cannula assembly, the CMA 470 can collect from four sources into four vials simultaneously. With the dual cannula assembly it can collect from two sources into two vials. There is also an option to collect samples in open vials.
The reusable carton provides excellent protection should it be necessary to transport the instrument or store it for a long period of time. Packing list The CMA 470 Refrigerated Fraction Collector, Ref No 800 2770 consists of: Refrigerated Fraction Collector equipped with Holder for Single Cannula External Power Supply...
Once the plug has been connected to a wall socket, the instrument is ready for use. Control Panel Figure 4.1 Control panel Locate the following items on the control panel of the CMA 470 Refrigerated Fraction Collector. See figure 4.1 Item Description 1.
Rear Figure 4.2 Rear The following are located on the rear of the CMA 470 Refrigerated Fraction Collector, see Figure 4.2 Item Description 1. USB Socket for USB connection to PC or other device with USB host port. 2. RS232 Socket for RS232 interface for connection to PC or other device with serial port.
Cassette Magazine Figure 4.4.1 Cassette magazine, closed lid Figure 4.4.2 Cassette magazine, open lid Locate the following items for the cassette magazine of the CMA 470 Refrigerated Fraction Collector. See Figures 4.4.1 and 4.4.2 Item Description 1. Lid Lid to keep the cooling and to protect from light.
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Step sequences for small vials: Figure 4.5.1 Numbering of the cassette positions for single, dual and quadruple cannulae NOTE: When three cannulae are in use, numbering is as for four cannulae. Every fourth position will be empty. Step sequences for large vials: Figure 4.5.2 Numbering of the cassette positions for single and dual cannulae NOTE: When collecting into large vials up to 2 cannulae can be used simultaneously.
Press the cassette button to be able to release all. Figure 4.5.3 Removal of cassette Warning: Risk for instrument damage. Only use vial cassettes for CMA 470 Collector. These cassettes are equipped with blue lift handles. Cassettes belonging to CMA/170 Collector can not be used, they will damage the instrument.
Single collect Assembly Fig. 4.8.1 Setting up Fig. 4.8.2 Setting up Fig. 4.8.3 Setting up Single Holder for Single Single Cannula for Cannula for Septa Cannula Tubing 4.8.1 Place the Holder for Single Cannula (1) so the tap (2) fit into the loop (3) in the right side slot (4) on the Supporting Arm.
Dual and Quadruple Collect Assembly Fig. 4.9.1 Setting up Fig. 4.9.2 Setting up Fig. 4.9.3 Setting up Quadruple Holder Quadruple Quadruple Cannulae for Cannulae for Septa Cannulae Tubing 4.9.1 Place the Holder for Dual or Quadruple Cannulae (1) on the Supporting Arm so that the Multi Holders for cannula (2) fit into the left slot (3) and the two holes (3).
4.10 Basic Functions The vial cassettes rotate clockwise for positioning and sample collecting. • The Holder for Single Cannula maneuver the cannula into one of two • collection positions, an inner and outer position. The Holders for Dual and Quadruple cannulae have fixed positions for 2 •...
OPERATING INSTRUCTIONS The CMA 470 Refrigerated Fraction Collector is easy to use. It is advisable to read this user manual carefully before starting to use the instrument. The CMA 470 Refrigerated Fraction Collector CMA can collect fractions from up to four sources into four vials simultaneously. The cannulae can be individually fitted and removed.
Set temperature NOTE: Turn on the cooling approximately 30 minutes before use. The cooling capacity is -15 °C from environmental temperature or better. Turn the Selection Knob to move the cursor to the lowest row. Press the Selection Knob to activate the temperature setting. Turn the Selection Knob to set the desired temperature.
Set fraction time Turn the Selection Knob to move the cursor to the third row. Press the Selection Knob to activate the position for the number of hours. Turn the Selection Knob to set the desired value. Press the Selection Knob to acknowledge. The curser moves to the data field to the position for the number of minutes.
Multi Holder can be moved from the Outer to Inner vial row and back (Single mode) with commands from the Menu. Press the front panel buttons Cassette, Step and the Menu/Manual commands Up/Down and In/Out when setting up the CMA 470 as described in chapter 4.8 and 4.9. Press the Menu button.
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Figure 6.1 Menu options Figure 6.2 Menu Manual operations The menu functions Set-Up and Info are only for maintenance and service purpose. Information can be found in the technical manual.
70% alcohol. Periodic wipe off the cooling compartment. Storage If the CMA 470 Refrigerated Fraction Collector will not be used for a significant length of time: Disconnect the mains supply Clean the instrument Wipe off the cooling compartment.
Warranty CMA/Microdialysis AB (called CMA) guarantees all components of the CMA 470 Refrigerated Fraction Collector to be free from defects of material and workmanship for a period of one year after initial purchase. For warranty service or repair, all CMA products must be returned to CMA or to an authorized representative.
10 TROUBLESHOOTING If the CMA 470 Refrigerated Fraction Collector does not function, check that: the input voltage and frequency correspond to the information given on the nameplate on the bottom of the instrument. the wall socket is live. the power cables are undamaged and properly connected both to the wall socket and to the instrument.
European Union customers: Contact your local CMA Microdialysis representative or your local authority for guidance. 13 ORDER INFORMATION Ref No CMA 470 Refrigerated Fraction Collector 800 2770 Including: Cassette for Small Vials, 4 pcs Holder for Single Cannula Holder for Dual Cannulae...
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CMA Microdialysis AB Head Office, Sweden Torshamnsgatan 30A, SE-164 40 Kista, Sweden Tel: +46 8 470 10 00 • Fax: 46-8-470 10 50 E-mail: support@hbiosci.com • www.microdialysis.com Harvard Apparatus 84 October Hill Road Holliston, MA 01746 USA Phone Orders: 800-232-2380 • Fax: 508-429-5732 E-mail: support@hbiosci.com •...
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