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Harvard Bioscience Biochrom Libra S6+ User Manual

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Libra S6
User Manual
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Libra S6
80-5001-20
5061-071 Rev 1.0

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  • Page 1 Libra S6 User Manual Libra S6 80-5001-20 5061-071 Rev 1.0...

  • Page 2: Table Of Contents

    Table of Contents Essential Safety Notes ..............................4 Unpacking, Positioning, Installation ..........................4 Warranty and Repair ................................5 Technical Specifications ……………………………………………………………………………………………………………….6 Operation..……………………………………………………………………………………………………………………………….7 Introduction ……………….…………………………………………………………………………………………………………7 Sample Handling Tips ..……………………………………….……………………………………………………………………7 Keypad and Display ..…………….…………………………………………………………………………………………………8 Software Style …………..……………………………………..……………………………………………………………………9 Applications Folder…………………………………………………………………………………………………………………….10 Applications…………………………………………………………………………………………………………………………11 Single Wavelength…………………………………………………………………………………………...………………11 Concentration…………………………………………………………………………………………………………………13 Wavescan…..………………………………………………………………………………………………………………...16 Simple Kinetics……………………………………………………………………………………………………………….19 Standard Curve……………………………………………………………………………………………………………….22 Multiple Wavelength………………………………………………………………………………………………………….29 Absorbance Ratio…………………………………………………………………………………………………………….31...
  • Page 3 Delete a Method …………………………………………………………………………………………………………………..58 Lock Method………………………………………………………………………………………………………………………..58 Unlock Method……………………………………………………………………………………………………………………..59 Favourites…………………………………………………………………………………………………………………………..60 Saving Data onto a USB Memory Stick ………………………………………………………………………………………...61 Utilities Folder ………………………………………………………………………………………………………….……………..63 Date and Time …………………………………………………………………………………………………………………….64 Regional …………………………………………………………………………………………………………………………...64 Export Data ………………………………………………………………………………………………………………………..65 Preference …………………………….…………………………………………………………………………………………..65 Contrast ……………………………………………………………………………………………………………………………66 About ……………………………………………………………………………………………………………………………….66 Print Via Computer ……………………………………………………………………………………………………………………67 Installation ………………………………………………………………………………………………………………………….67 Test Tube and Heated Cell Holder Installation …………………………………………………………………………………….68 Accessories ……………………………………………………………………………………………………………………………69 Cleaning and General Care of the Instrument ……………………………………………………………………………………..69...

  • Page 4: Essential Safety Notes

    Essential Safety Notes There are a number of warning labels and symbols on your instrument. These are there to inform you where potential danger exists or particular caution is required. Before commencing installation, please take time to familiarize yourself with these symbols and their meaning. Caution (refer to accompanying documents).

  • Page 5: Warranty And Repair

    Warranty and Repair Biochrom warrants this instrument for a period of 12 months (1 year) from the date of purchase. Where appropriate, Biochrom will repair or replace the unit for defects of workmanship or materials. This warranty does not extend to damage resulting from misuse, neglect, or abuse, normal wear and tear, or accidental damage.

  • Page 6: Technical Specifications

    Technical Specifications Wavelength range 325nm to 1100nm Monochromator Flat grating Wavelength calibration Automatic upon switch on Spectral bandwidth <7nm Wavelength accuracy ± 2nm Wavelength reproducibility ± 1nm Light sources Pulsed Tungsten halogen Detector CMOS array Photometric range - 0.300 to 2.500A, 0.3 to 199%T ±...

  • Page 7: Operation

    Operation Introduction Your spectrophotometer is a simple-to-use visible instrument. It has no moving parts, which is the basis of the rapid scanning operating system. It has been designed to meet the routine spectroscopy needs of customers requiring a compact instrument that is easy to use. The product is reliable and requires low maintenance. The user interface is built around folders which are displayed on the home page when the instrument is turned on.

  • Page 8: Keypad And Display

    Keypad and Display The back-lit liquid crystal display is very easy to navigate around using the alphanumeric entry and navigation arrow keys on the hard wearing, spill proof membrane keypad. Alphanumeric Keys Escape/Cancel On/Standby OK Key Set Reference Arrow Keys Red light will appear View Options when USB is inserted...

  • Page 9: Software Style

    Software Style The user interface is built around having folders of files which are displayed on the home page when the instrument is switched on. Different folders are numbered and opened by using the associated number key on the keypad. Folder Keypad Number Description...

  • Page 10: Applications Folder

    The Applications Folder Function Keypad Number Description Absorbance or %T (transmission) at a single user defined wavelength. Concentration measurement at a single wavelength based on a simple Factor entered or calculated from a single standard. Wavelength scan between two user-defined wavelengths. Range 330-950 nm, with user configurable peak finding function.

  • Page 11: Applications

    to store its last settings. If the “History” parameter is turned off, all parameters and options will return to their default settings when you leave that application. (Unless it has been saved as a method). Applications Single wavelength This makes simple absorbance (A) and % transmission (%T) measurements on samples, measuring the amount of light that has passed through a sample relative to a reference (this can be air).
  • Page 12 Results Screen The result at the selected wavelength is displayed on screen. Use the left and right arrows to move the cursor and display the value at the cursor position (+/- 15nm from set wavelength). Press Cancel to return to the Applications Folder.

  • Page 13: Concentration

    Concentration This makes simple concentration measurements on samples, by measuring the amount of light that has passed through a sample relative to a reference (this can be air). Concentration is obtained by multiplying the measured absorbance at a specific wavelength by a factor. The factor may be known in advance, or may be calculated by the instrument by measuring a standard of known concentration.
  • Page 14 Step 6 Select whether or not to adjust the name of your sample before each new run, using left and arrow keys to select Yes or No. Step 7 To enter the results screen with the selected parameters press OK or Cancel the selections and return to the Applications Folder by pressing Cancel...
  • Page 15 Press to return to the Applications Folder. Press to display available Options which are described below. Note: If below zero or negative the concentration value calculated will be shown as ---. A measured negative absorbance (reference measurement higher absorbance than measured sample absorbance) would require a negative factor to create a positive concentration.

  • Page 16: Wavescan

    Wavescan An absorption spectrum can be obtained from your instrument, enabling simple identification of peak height and position. The procedure is as follows: Step 1 Set start wavelength by using keypad numbers or left and right arrows. Press the down arrow key. Step 2 Set end wavelength by using keypad numbers or left and right arrows.
  • Page 17 Results Screen A graph of the wavescan is displayed, along with a table of Absorbance/%T at each peak. Use the left and right arrows to move the cursor along the graph. When it reaches a peak the peak height and width of the peak is displayed at the top of the screen.
  • Page 18 Peak Detect on Zoom: Determines whether peaks are re-assessed and tabulated when the user zooms into a region of the wavescan. If off, it leaves the peak detection as determined on the un-zoomed display. Sort peaks by…: Determines the sequence that peaks are reported by.

  • Page 19: Simple Kinetics

    Simple Kinetics Kinetics studies, where the change in absorbance needs to be followed as a function of time at a fixed wavelength, can be readily performed. Reagent test kits are routinely used for the enzymatic determination of compounds in food, beverage and clinical laboratories by measuring NAD / NADH conversion at 340 nm.
  • Page 20 Step 7 Units: The user can enter a text string up to 8 characters long. To access a list of pre-defined units press the Options key and then use the left/right arrows (µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or none). These units can also be edited once OK pressed.
  • Page 21 Press Cancel to return to the Applications Folder. Press to display available Options. Options (select using key pad numbers) 1. Return to parameter 1 screen (step 1 above). 2. Print data on the results screen via selected method. 3. Print all the data. 4.

  • Page 22: Standard Curve

    Standard Curve The construction of a multi-point calibration curve from standards of known concentration to quantify unknown samples is a fundamental use of a spectrophotometer; this instrument has the advantage of being able to store this curve as a method, using up to 9 standards. To include a zero concentration standard, include this in the number of standards to be entered and enter 0.00 for concentration;...
  • Page 23 Step 5 Select the calibration mode: either Standards (measure prepared standards), Manual (keypad data entry), or New Standards (same as Standards but forces new standards to be measured when the method is re-run even if history is enabled and standards have already been measured) Press the down arrow.
  • Page 24 Calibration Screen (with Replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert the reference and press the reference key . This will be used for all subsequent samples until changed. Step 12 Insert the standard (use C to clear previously stored results before measuring.
  • Page 25 Step 15 Press Next when all standards have been entered. If any duplicate or non-monotonic (increasing entries) are present the unit will beep and highlight the incorrect entry or press back return to the Parameter screen. Step 13 Select whether or not to adjust the name of your sample before each new run, using left and arrow keys to select Yes or No.
  • Page 26 A graph will display the results and the fitted curve as the measurements are input. Use the up and down arrows to select a standard to be repeated if a poor reading has been obtained. Use C to clear the previous reading.
  • Page 27 Step 20 Press to display the replicates entry box. Use C to clear previously stored results before measuring. You will need to use the down arrow key to clear each line. Step 21 Insert the reference. Press the reference key Step 22 Press to measure the standard and store the...
  • Page 28 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected method. 3. Toggle graph on/off. Displays calibration graph, cursors give values for last measured sample. 7. Sample number – add a prefix to the sample number and reset the incrementing number to the desired value.

  • Page 29: Multiple Wavelength

    Multiple Wavelength This makes up to 5 absorbance measurements on the same sample. The procedure is as follows: Step 1 Select the number of wavelengths. Press the down arrow key. Step 2 Enter the first wavelength using either the number keys or the left and right arrows.
  • Page 30 Results Screen A scan plot covering the range of wavelengths selected (with cursors at the relevant wavelengths) and a table of values is displayed. Press to return to the Applications Folder. Press to display available Options. Options (select using key pad numbers) 1.

  • Page 31: Absorbance Ratio

    Absorbance Ratio This makes simple absorbance ratio measurements on samples, measuring the amount of light that has passed through a sample relative to a blank (this can be air) at two wavelengths. The procedure is as follows: Step 1 Enter the first wavelength by using the keypad numbers or the left and right arrows.
  • Page 32 Step 7 (Calculate Dilution Factor) Press the options key Enter the volume of the sample (range 0.01 – 9999), using the keypad numbers. Press the down arrow. Enter the volume of diluent (range 0.01-9999) by using the keypad numbers. Press OK to calculate the dilution factor and return to the Parameters screen (or press Back to cancel selections).
  • Page 33 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected method. 3. Toggle graph on/off. Graph shows a wavescan plot across the selected wavelengths in place of the individual wavelength. 7. Sample number – add a prefix to the sample number and reset the incrementing number to the desired value.

  • Page 34: The Life Science Folder

    The Life Science Folder This folder contains five different protein methods outlines below: Method Keypad Number Description Protein Determination at 562 nm Protein Determination at 592 nm Protein Determination at 750 nm Protein Determination at 546 nm Cell Culture OD600 with correction factor 34 | P a g e...

  • Page 35: Applications (Protein Determination)

    Applications Protein Determination Protein Determination at 595, 546, 562 and 750 nm  The Bradford method depends on quantitating the binding of a dye, Coomassie Brilliant Blue, to an unknown protein and comparing this binding to that of different, known concentrations of a standard protein at 595 nm; this is usually BSA, bovine serum albumin.

  • Page 36: Bca

    The procedure is as follows: Step 1 Wavelength for this stored method is pre-set to 562nm. Step 2 Enter the number of standard concentration points (1- 9) to be used in the curve using the keypad numbers or left and right arrows. Press the down arrow key.
  • Page 37 Step 6 (if Standards selected) Select the number of replicates using the left and right arrows. This determines the number of standards to be measured and averaged at each standard concentration point. Can be OFF (1), 2 or 3. Step 7 Press Next to enter the Standards screen or press Cancel...
  • Page 38 Calibration Screen (Replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert the reference sample. Press the reference key . This will be used for all subsequent samples until changed. Step 12 Insert the standard (use C to clear previously stored results before measuring).
  • Page 39 Repeat Step 15 for all replicates and standards. A graph will display the results and the fitted curve as the measurements are input. Use the up and down arrows to select a standard to be repeated if a poor reading has been obtained. Use C to clear the previous reading.
  • Page 40 Press to return to the Life Science Folder. Press to display available Options. Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected method. 3. Toggle graph on/off. Displays the calibration graph, cursors give values for last measured sample.

  • Page 41: Bradford

    Bradford The procedure is as follows: Step 1 Wavelength for this stored method is pre-set to 595 Step 2 Enter the number of standard concentration points (1-9) to be used in the curve using the keypad numbers or left and right arrows. Press the down arrow key.
  • Page 42 Step 5 Select the calibration mode, either standards (measure prepared standards), manual (keypad data entry), or new standards (same as Standards but forces new standards to be measured when the method is re-run even if history is enabled and standards have already been measured). Step 6 (if standards selected) Select the number of replicates using the left and right arrows.
  • Page 43 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert the reference and press the reference key . This will be used for all subsequent samples until changed. Step 12 Insert the standard (use C to clear previously stored results before measuring) Press to measure the standard and store the...
  • Page 44 Use the up and down arrows to select a standard to be repeated if a poor reading has been obtained. Use C to clear the previous reading. Step 16 Press to accept the calibration and go to the Results screen or press Back to return to the Parameters screen.
  • Page 45 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected method. 3. Toggle graph on/off. Displays the calibration graph, cursors give values for last measured sample. 7. Sample number – add a prefix to the sample number and reset the incrementing number to the desired value.

  • Page 46: Lowry

    Lowry The procedure is as follows: Step 1 Wavelength for this stored method is pre-set to 750 Step 2 Enter the number of standard concentration points (1-9) to be used in the curve using the keypad numbers or left and right arrows. Press the down arrow key.
  • Page 47 Step 5 Select the calibration mode, either standards (measure prepared standards), manual (keypad data entry), or new standards (same as Standards but forces new standards to be measured when the method is re-run even if history is enabled and standards have already been measured) Step 6 (if standards selected) Select the number of replicates using the left and right arrows.
  • Page 48 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert the reference and press the reference key . This will be used for all subsequent samples until changed. Step 12 Insert the standard (use C to clear previously stored results before measuring) Press to measure the standard and store...
  • Page 49 Use the up and down arrows to select a standard to be repeated if a poor reading has been obtained. Use C to clear the previous reading. Step 16 Press to accept the calibration and go to the Results screen (see below) or press Back return to the Parameters screen.
  • Page 50 Press to return to the Life Science Folder. Press to display available Options which are described below. Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected method. 3. Toggle graph on/off. Displays the calibration graph, cursors give values for last measured sample.

  • Page 51: Biuret

    Biuret This procedure is as follows: Step 1 Wavelength for this stored method is pre-set to 546 Step 2 Enter the number of standard concentration points (1- 9) to be used in the curve using the keypad numbers or left and right arrows. Press the down arrow key.
  • Page 52 Step 6 (if standards selected) Select the number of replicates using the left and right arrows. This determines the number of standards to be measured and averaged at each standard concentration point. Can be OFF (1), 2 or 3. Step 7 Press Next to enter the Standards screen or press Cancel...
  • Page 53 Repeat step 12 for all standards. A graph will display the results and the fitted curve as the measurements are made. Use the up and down arrows to select a standard to be repeated if a poor reading has been obtained. Use C to clear the previous reading.
  • Page 54 Calibration (Manual entry and New Standards) Shows previously entered calibration values and allows values to be entered via the keypad. The highlighted box can be edited in order to enter an absorbance value corresponding to a given concentration value using the keypad numbers. Range 0.001 to 9999.

  • Page 55: Od600

    OD600 Bacterial cell cultures are routinely grown until the absorbance at 600 nm (known as OD600) reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6. It is important to note that for turbid samples such as cell cultures, the absorbance measured is due to light scattering, and not the result of molecular absorption.
  • Page 56 Step 5 (if cells/ml selected) Enter the factor using the keypad numbers. Range 0.00 to 9999. Use the C button to clear the last digit entered. Press the down arrow key. Step 6 (if cells/ml selected) Select the multiplier using the left and right arrows. Options are 1000 or 1,000,000.

  • Page 57: Methods And Favourties Folder

    Methods and Favourites Folder These folders are the storage locations for any user modified Applications (Methods) that are saved in the Options menu. Both are accessible from the home folders page. Methods These are further storage folders enclosed in the top level of the Methods Folder. Up to 9 methods may be stored in each folder.

  • Page 58: Delete A Method

    Use the left and right arrow keys to save the methods to a methods folder (1-9), in favourites, or to a USB. While in the methods folder, press display the following options: Delete Method Press 1 to select delete method. Select the method to be deleted using the left and right arrows.

  • Page 59: Unlock Method

    Unlock Method Press 3 to select unlock method. Select the method to be unlocked using the left and right arrows. Press the down arrow key. Enter the pass code using the keypad numbers or left and right arrows. Press to unlock the method or cancel to return to the Methods folder.

  • Page 60: Favourites

    Favourites This folder enables the user to quickly select any frequently used Methods. Up to 9 Methods may be stored in this folder. Operation is identical to the Methods Folder. Saving a Method to Favourites In the results screen of the application, press key to display the options.

  • Page 61: Saving Data Onto A Usb Memory Stick

    Saving Data onto a USB Memory Stick When a USB memory stick is inserted into the connector in front of the instrument an audible click will be heard as the stick is recognized. Whenever a USB stick is inserted, all data will automatically be saved to the USB memory stick. The format used is selected from the utilities menu under printer options.
  • Page 62 Data is stored on the USB memory stick under the following directory structure \Instrument serial no\PVC Double clicking on a file opens it into the PVC application from which it can be exported or saved. Full details on the use of PVC are covered in the PVC user manual 62 | P a g e...

  • Page 63: Utilities Folder

    Utilities Folder Folder Keypad Description Number Set correct time and date Select preferred language and number format Export output options Select screen layout (themes) and history Adjust screen contrast & brightness Serial number and software version 63 | P a g e...

  • Page 64: Date And Time

    1. Date and Time Enter the day using the keypad numbers or left and right arrows. Press the down arrow key. Enter the month as above. Press the down arrow key. Enter the year. Press the down arrow key. Enter the hour. Press the down arrow key.

  • Page 65: Export Data

    3. Data Output Select whether auto-print is on or off using the left and right arrows. When auto-print is on the results are automatically printed after a measurement is taken. When it is off, printing has to be initiated manually. This can also be set using the Options in each application or method.

  • Page 66: Contrast

    5. Contrast Ambient light and temperature can affect the display. This function can optimize the display for local conditions. Adjust the contrast using the left and right arrows. Press the down arrow key. Adjust the brightness using the left and right arrows.

  • Page 67: Print Via Computer

    Print Via Computer (PVC) This PC application running on a Windows computer can be used to transfer result data from an attached instrument. The result data transferred can be printed or stored in a variety of formats including Excel spreadsheet, graphical (EMF), comma delimited text (CSV), tab delimited text (TXT), rich text format (RTF) and PVC.

  • Page 68: Test Tube And Heated Cell Holder Installation

    Test Tube and Heated Cell Holder Installation 1. Lay the instrument upside down on a soft surface. 2. Unscrew the two screws and remove the cuvette cell holder by tilting the holder to allow the cell ring to clear the baseplate.

  • Page 69: Accessories

    Accessories Description Catalog Number Heated cell holder 80-3007-13 Test tube holder 80-3007-12 Cleaning and General Care of the Instrument External cleaning Switch off the instrument and disconnect the power cord. Use a soft damp cloth. Clean all external surfaces. A mild liquid detergent may be used to remove stubborn marks. Changing cell holder or removal for cleaning This can be removed by undoing the appropriate screws on the bottom of the instrument.

  • Page 70: Icon Glossary

    Icon Glossary Status Bar Icons Meaning Measurement in progress (lamp lit). The icons are cycled through to indicate the lamp coming up to full brightness and then shown in reverse when the lamp is turned off. Heated cell holder at temperature. Heated cell holder fitted but not enabled.
  • Page 71 Preferences setup. Regional settings. Data export setup defines the settings for the “Print” output to PC and USB memory stick. Spectro Blocks game (only available if enabled in preferences). Sudoko game (only available if enabled in preferences). Method storage folder. Menu folder.
  • Page 72 China Biochrom Room 1902E 19F, Building B Zhong Shan Plaza 1065 West Zhig Shan Road Changning District Shanghai, China 200051 Telephone +86 21 2230 5128 Email support@hbiosci.com Website www.biochrom.co.uk United Kingdom Biochrom Building 1020 Cambourne Business Park Cambourne Cambridge, CB23 6DW, United Kingdom Telephone +44 (0) 1223 423 723 +44 (0) 1223 4220 164...

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