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Summary of Contents for Izon ZENCO BETA
- Page 1 Z E N C O B E T A U S E R M A N U A L www.izon.com...
- Page 2 Izon Science Limited provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorisation of Izon Science Limited.
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Page 3: Table Of Contents
LIVE DATA AND DATA ANALYSIS ........................29 Data Visualisation During a Run ......................... 29 Data Analysis ................................29 CALIBRATION AND TROUBLESHOOTING ..................... 30 Calibration ..................................30 Troubleshooting ................................31 Maintenance ................................33 CONTACT US ................................35 qEV Zenco Beta User Manual... -
Page 4: Definitions And Writing Conventions
The mobile phase may be gaseous or liquid. The total volume between the upper and lower frits. Column Volumes can be found Column Volume in their respective qEV user manual. Method The programmable workflow used to run a qEV column. www.izon.com... -
Page 5: Safety And Hazards
100 mL/min Outlet Tubing Quantity Outer Diameter: 1.6 mm Outlet Tubing Diameter Outlet Inner Diameter: 0.75 mm Pressure Range 0 ~ 5 MPa Do not attempt to run the qEV Zenco outside of these conditions. qEV Zenco Beta User Manual... -
Page 6: Hazards
Personal Protective Equipment (PPE) when handling these substances. *Izon is transitioning from the use of sodium azide to ProClin 200 for the storage of qEV columns. For information on how to identify which storage buffer is in your column, visit support.izon.com... -
Page 7: Safety-Data-Sheet-Sds
(1:10 dilution of household bleach is recommended). Dispose of the following potentially contaminated materials in accordance with laboratory local, regional, and national regulations: Biological samples Reagents Used reaction vessels or other consumables that may be contaminated qEV Zenco Beta User Manual... -
Page 8: Data Sheets
(e.g., buzzing when touched), if water ingresses into the instrument, the supply voltage flashes (indicating unstable power supply), electrical parts display damage, or if the instrument fails to function as expected. Maintenance and repair must be carried out under the guidance of Izon support staff when the instrument is unplugged. - Page 9 Equipment and/or Consumables are recycled or disposed of in accordance with this and/or any other relevant laws and regulations in the countries where the instrument is being recycled or disposed of. Contact your local Izon Science representative for more information. qEV Zenco Beta User Manual...
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Page 10: Introduction To The Qev Zenco
This instrument is for use by professional personnel only who are familiar with operating bioprocessing equipment and managing biological materials. For verification of the entire system, it is recommended that cGLPs are followed to ensure reliable analyses. www.izon.com... -
Page 11: Instrument Specifications
Column holder Cond Conductivity sensor Air sensor Pressure sensor Pump B P V Back pressure valve Indicator lights Outlet valve pH sensor Flow director valve Inlet valve PP centrifuge tube Power switch Power socket Serial port qEV Zenco Beta User Manual... - Page 12 Figure 1: Components of the front of the Zenco Unit. The flow path is represented in blue. Figure 2: Components on the side and rear of Zenco unit. In addition to the Zenco unit, additional components are required for operation. A full list of provided components can be found in Table 5 on page 12. www.izon.com...
- Page 13 Pre-made outlet tubing (800 mm 10-32 PEEK male fingertight connector x 1/16 PTFE tubing & 1/16 PEEK female luer lock (located in the pump head) fingertight connector) x 4 Figure 3: All components provided with the Zenco. qEV Zenco Beta User Manual...
- Page 14 Freshly prepared, filtered (0.22 µm) and degassed buffer. Refer to your qEV user manual for more information about buffers Freshly prepared 0.5 M NaOH solution for column cleaning Fresgly prepared 1 M NaOH solution for system cleaning 20% EtOH DI water Large vessel for waste collection www.izon.com...
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Page 15: Assembly And Setup Instructions
Izon instruments are only to be operated with Izon supplied leads and power supplies. Failure to use the correct power supply may result in invalid operation. - Page 16 To set up the pump, remove the 25 mL centrifuge tube from the front of the instrument, add 15 mL of 20% ethanol and re-attach the centrifuge tube. Weekly maintenance requires replacement of the 20% ethanol attached to the pump. The volume should be topped up if it falls to less than 10 mL. www.izon.com...
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Page 17: Zenco Software
Zenco Software Software Installation Instructions To access the software installation instructions, please head to support.izon.com/how-do-i-download- the-qev-zenco-software Figure 5: qEV Zenco software main screen. qEV Zenco Beta User Manual... - Page 18 Access then to either Change Login Password or Change Signature Password to change the login or signature password respectively. Software Indicator Lights The software shows 4 alert lights directly above the flow path diagram, as shown in Figure Figure 7: Software indicator lights. www.izon.com...
- Page 19 The inlet tubing connects to the inlet valve (V1), and then passes through the pump. From the pump, two tubes connect to the pressure sensor (P1). After this, a single tube exits P1 and passes through the air sensor before entering V2 via the channel labelled ‘IN’. qEV Zenco Beta User Manual...
- Page 20 The flow will then enter the outlet valve (V3) before exiting through the outlet tubing into a designated vessel. The back pressure valve (BPV) can be connected between the UV and pH if required (see Section 5.4: Connecting and Disconnecting the Back Pressure Valve). www.izon.com...
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Page 21: Operating Instructions
If you are running your protocol through manual control, you can set your column volume by clicking on Parameters > Settings. Select the Basic settings tab, shown in Figure 9, and enter the Column Volume of your qEV column – this value can be found in your respective qEV User Manual. qEV Zenco Beta User Manual... - Page 22 Flow rates of 70 mL/min or lower are suitable for continuous operation. Figure 10: Set system pump pop-up Click Run to open the Manual Mode Run Confirmation box. Figure 11: Manual control buttons. www.izon.com...
- Page 23 Click Start to begin timer. This can be useful when running the system manually. The Stop button can be used to stop the timer early. Figure 13: Timer pop-up. qEV Zenco Beta User Manual...
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Page 24: Preparing Your Qev Zenco For Use With Qev Columns
Remove the connector cap and then unscrew the qEV column cap and fill the column with buffer until it reaches the upper frit (i.e., is level with the top edge of the column). Screw the column connector cap back on, forcing buffer up through the connector junction. www.izon.com... -
Page 25: Set Upper Pressure Limit And Leakage Test
Run a manual flow check of buffer in the forward and bypass directions at 10 mL/min to ensure that there are no leaks at any connections. If any leaks are detected, please tighten the relevant connectors. Once no leaks have been detected, press Stop. If leaks continue to be detected, please contact Izon Support for further guidance. - Page 26 Enter your desired flow rate and volume. Set the flow direction to Forwards for column priming. Select whether you wish to “auto zero” the UV sensor during this step. Figure 17: Column priming tab. www.izon.com...
- Page 27 Please note that the flow rate can be higher than gravity for column cleaning. Figure 19: Column cleaning tab. Your method is now ready to be saved by clicking Save at the top of the screen. Select Back to return to the homepage. qEV Zenco Beta User Manual...
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Page 28: Starting A Run With A Method
Turn the power switch to the “O” position to put the the system into standby mode. If the power cord of the instrument is unplugged from the socket or the power socket is turned off, the instrument will enter storage mode. www.izon.com... -
Page 29: Live Data And Data Analysis
Signature can be used to sign your analysis using your login. To compare curves between runs, go to the Curve Comparison tab. To generate a PDF report, use the PDF Report tab and modify the settings there to your needs. qEV Zenco Beta User Manual... -
Page 30: Calibration And Troubleshooting
Use 1413 µS/cm, 12.88 mS/cm, and 111.3 mS/cm conductivity standard for CP2, CP3, and CP4 respectively following the same instructions as step 6. The conductivity will now be calibrated. Test the conductivity using a solution of known conductance. www.izon.com... -
Page 31: Troubleshooting
If this is successful then it will display “Home is found”. Troubleshooting No Power Displayed When System is Powered On Check that the power supply cables are not loose. Unplug and re-plug the power cord. Reboot the system. Check if the cooling fan is running. qEV Zenco Beta User Manual... - Page 32 If the cooling fan is not running or if the reboot does not fix the issue, please get in touch with Izon Science Support Alarm Occurs When the Zenco is First Turned On Check that the Link light is green. If it is red, a communication error is occurring.
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Page 33: Maintenance
Salt crystals may have formed in the optical path of the UV flow cell. Flush the system with DI water for 10 minutes at 50 mL/min with the column bypassed. Check whether the UV reading returns to normal levels. If this does not occur, please contact Izon Science Support. -
Page 34: Section 6.2: System
If the operating time is greater than 4,000 hours, replace the seal ring and replace the flow cell as necessary. Back Pressure Valve Clean the BPV as necessary by connecting it to the Zenco unit and flushing it with water at 10 mL/min for 5 minutes. www.izon.com... -
Page 35: Contact Us
When reporting Zenco issues to Izon support, please provide the serial number of the Zenco, which can be found on the rear of the Zenco as in...
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