Subscribe to Our Youtube Channel
Related Manuals for Thermo Scientific Steri-Cult 3307 Series
Summary of Contents for Thermo Scientific Steri-Cult 3307 Series
- Page 1 Steri-Cult Model 3307 and 3310 Series CO Incubator Controlled RH with Sterilization Cycle Operating and Maintenance Manual 7023307 Rev. 28 Visit us online to register your warranty www.thermoscientific.com/labwarranty...
- Page 2 Added pilasters and shelf channels to Parts List 11/24/10 Corrected typo in chart above: 3308/3310, medium/large 25128/IN-3882 4/7/10 Missed correction from water bottle change (pgs 1-10, 7-3 & 7-4) 25480/SI-10033 11/4/09 Common shelf channel (pg 1-7 & 1-8 artwork and 11-1 parts list) Thermo Scientific 7023307 Steri-Cult...
- Page 3 Caution If the incubator is not used in the manner specified in this operating manual, the protection provided by the equipment design may be impaired. Intended Use: The Thermo Scientific Steri-Cult CO Incubators are intended to maintain an optimal environment for the incubation of cells and tissues for scientific research and clinical applications.
- Page 4 Always use the proper protective equipment (clothing, gloves, goggles, etc.) 4 Always dissipate extreme cold or heat and wear protective clothing. 4 Always follow good hygiene practices. 4 Each individual is responsible for his or her own safety. Thermo Scientific 7023307 Steri-Cult...
- Page 5 Canada Statement: This ISM device complies with Canadian ICES-001. Cet appareil ISM est conforme à la norme NMB-001 du Canada. 7023307 Steri-Cult Thermo Scientific...
- Page 6 We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...
- Page 7 • Repairs to parts or systems resulting from unauthorized unit modifications. • Any labor costs other than that specified during the parts and labor warranty period, which may include additional warranty on CO sensors, blower motors, water jackets, etc. 7023307 Steri-Cult Thermo Scientific...
-
Page 8: Table Of Contents
System ......3-2 Calibrating Relative Humidity (RH) ......3-3 Thermo Scientific 7023307 Steri-Cult... - Page 9 Sterilization Cycle ..........8-1 viii 7023307 Steri-Cult Thermo Scientific...
- Page 10 Replacing the Fuses ........11-3 Replacing the CO and RH Air Filters ..... . .11-3 Thermo Scientific 7023307 Steri-Cult...
- Page 11 Table of Contents 7023307 Steri-Cult Thermo Scientific...
-
Page 12: Installation And Start-Up
Control Panel - Keypad, Displays & Indicators (Figure 1-3) • Fold-down Storage Area - Store manuals, markers etc. • Leveling Feet - Used to level the unit Note The incubators are stackable. Information follows in this section. Thermo Scientific 7023307 Steri-Cult... -
Page 13: Inventory Management System
Humidity (RH) Display Mode Select Switch Mode Settings Calibrate Configuration Up Arrow Message Center CLASS 100 Enter Button Enter Down Arrow Left and Right Arrows Silence Button Silence Sterilization Button Alarm Indicator Enviro-Scan™ Figure 1-3. Control Panel Components 7023307 Steri-Cult Thermo Scientific... -
Page 14: Operation Of The Keypad
Run is the default mode which the incubator will normally be in during operation. Settings is used to enter system setpoints for incubator operation. Calibrate is used to calibrate various system parameters. Configuration allows for custom setup of various options. Thermo Scientific 7023307 Steri-Cult... -
Page 15: Displays
0.5 micron size or larger per cubic foot of air. 3 Upper Displays: The first upper display shows the temperature. The second display shows the percentage of CO . The third display shows the humidity percentage. 7023307 Steri-Cult Thermo Scientific... -
Page 16: Installing The Incubator
1. Unscrew and remove the 4 leveling feet from the unit to be stacked on top and lift it onto the base unit. Align all sides. Figure 1-4. Stacking Bracket Thermo Scientific 7023307 Steri-Cult... -
Page 17: Preliminary Cleaning
Fit each pilaster securely into the indentations at each side of the ceiling and the floor of the chamber. Figure 1-7 shows the diffuser pan, pilasters, and shelf channels. 2. Install the diffuser pan in the bottom of the unit with the flanges down. 7023307 Steri-Cult Thermo Scientific... - Page 18 Figure 1-10. Access Port Filter Installation Caution Filter MUST be installed inside the chamber or it will become plugged with condensation and inhibit the recovery of the RH system. Do not replace this filter with a solid cover of any kind. Thermo Scientific 7023307 Steri-Cult...
-
Page 19: Installing The Hepa Filter
HEPA filter carefully over the back edge of the metal lip. 5. Lift the front of the filter to the ceiling of the chamber. Push filter bracket back over the filter frame. Tighten the wingnuts. 6. Refer to Section 6 for HEPA filter maintenance. 7023307 Steri-Cult Thermo Scientific... -
Page 20: Leveling The Unit
Tap water is not recommended as it may contain chlorine, which can deteriorate the stainless steel. Tap water may also have a high mineral content, which would produce a build-up of scale in the pan. Thermo Scientific 7023307 Steri-Cult... -
Page 21: Filling The Humidity Water Bottle
Note For customer convenience, 12 feet of 1/4” I.D. vinyl tubing with two 3/8” hose clamps are included in the shipping materials for connecting the unit to the gas supply. 1-10 7023307 Steri-Cult Thermo Scientific... -
Page 22: Humidity And Loading The Chamber
2% above setpoint, the incubator microprocessor turns on dehumidification. This process pulls outside air in through the nebulizer and exhausts air through the access port. Humidity decreases. However, in the process, CO consumption increases. Ensure your tank and product are monitored. Thermo Scientific 7023307 Steri-Cult 1-11... - Page 23 Section 1 Installation and Start-Up 1-12 7023307 Steri-Cult Thermo Scientific...
-
Page 24: Incubator Settings
3. Press the up/down arrow key until the desired temperature setpoint is displayed. 4. Press Enter to save the setpoint. 5. Press the Mode key until the Run indicator lights for Run mode or press the right/left arrow keys to go to next/previous parameter. Thermo Scientific 7023307 Steri-Cult... -
Page 25: Setting The Overtemp Setpoint
3. Press the up or down arrow key until the desired Overtemp setpoint is displayed. 4. Press Enter to save the setting. 5. Press the Mode key until the Run indicator lights or press the right or left arrow to go to the next or previous parameter. 7023307 Steri-Cult Thermo Scientific... -
Page 26: Setting The Co Setpoint
On power-up or a temperature setpoint change, RH is delayed until the temperature is within 0.5°C of the setpoint. RH WAITS ON TEMP is displayed until this temperature is reached. Thermo Scientific 7023307 Steri-Cult... -
Page 27: Set Mode
CO2 XX.X% Press Enter Enter to save setting Numbers decrease Press to return to previous parameter Percent Numbers increase RH XX% Relative Humidity Press Enter Enter to save setting Numbers decrease Press to return to previous parameter 7023307 Steri-Cult Thermo Scientific... -
Page 28: Calibration
Temperature Stabilization Periods Startup - Allow 12 hours for the temperature in the cabinet to stabilize before proceeding. Presently Operating - Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding. Thermo Scientific 7023307 Steri-Cult... -
Page 29: Calibrating The Infrared Co System
3. Press the right arrow until “CO2 CAL XX.X%” appears in the message center. 4. Press the up/down arrow to adjust the display to match the independent instrument reading. 5. Press Enter to store the calibration. 6. Press the Mode key to return to Run mode. 7023307 Steri-Cult Thermo Scientific... -
Page 30: Calibrating Relative Humidity (Rh)
2. Press the right arrow key until “RH CAL XXX%” appears in the message center. 3. Press the up/down arrow to match the display to the independent instrument. 4. Press Enter to store the calibration. 5. Press the Mode key to return to Run mode. Thermo Scientific 7023307 Steri-Cult... - Page 31 Press Enter to Enter save the setting Numbers decrease Press to return to previous parameter Numbers increase Percent RH RH CAL XXX% Press Enter to Enter save the setting Numbers decrease Press to return to previous parameter 7023307 Steri-Cult Thermo Scientific...
-
Page 32: Configuration
After replacing the HEPA filter with a new one, clear the display and reset the timer by following the steps below. 1. Press the Mode key until the Configuration indicator lights. 2. Press the right arrow until NEW HEPA XXX is displayed in the message center. Thermo Scientific 7023307 Steri-Cult... -
Page 33: Setting The Replace Hepa Filter Reminder
HEPA XXX displays. This number is the remaining days before the specified filter replacement time runs out. For example, if 12 months was chosen in the REPLACE HEPA XX message screen, the NEW HEPA number would be 365 days. 7023307 Steri-Cult Thermo Scientific... -
Page 34: Setting An Access Code
OFF will not allow the temp alarms to trip the contacts. The factory setting is ON. 1. Press the Mode key until the Configuration indicator lights. 2. Press the right arrow until TEMP RELAY ON/OFF is displayed. Thermo Scientific 7023307 Steri-Cult... -
Page 35: Setting A Low Co Alarm Limit
3. Press up/down arrow to change the high CO alarm limit. 4. Press Enter to save the high CO alarm limit. 5. Press the Mode key to return to run mode or right/left to go to next/previous parameter. 7023307 Steri-Cult Thermo Scientific... -
Page 36: Enabling Co
3. Press up/down arrow to change the RH low alarm limit. 4. Press Enter to save the RH low alarm limit. 5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter. Thermo Scientific 7023307 Steri-Cult... -
Page 37: Setting A High Rh Alarm Limit
3. Press up/down arrow to toggle the setting ON or OFF. 4. Press Enter to save the setting. 5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter. 7023307 Steri-Cult Thermo Scientific... -
Page 38: Selecting A Primary Tank
3. Press up/down arrow to toggle the setting ON or OFF. 4. Press Enter to save the setting. 5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter. Thermo Scientific 7023307 Steri-Cult... - Page 39 3. Press up/down arrow to change the RS485 address. 4. Press Enter to save the RS485 address. 5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter. 7023307 Steri-Cult Thermo Scientific...
- Page 40 Section 4 Configuration Thermo Scientific 7023307 Steri-Cult...
- Page 41 CO2 alarm relay ON CO2 RELAY ON/OFF Press Enter to CO Alarm Relay Enter save the setting CO2 alarm relay OFF Press to return to previous parameter refer to next page 4-10 7023307 Steri-Cult Thermo Scientific...
- Page 42 Section 4 Configuration Thermo Scientific 7023307 Steri-Cult 4-11...
- Page 43 Section 4 Configuration 4-12 7023307 Steri-Cult Thermo Scientific...
-
Page 44: Alarms
15 minutes. The temperature alarms are disabled when the temperature setpoint is 10°C. The CO alarms are disabled when the CO set point is 0.0%. RH alarms are disabled when the setpoint is 0%. Thermo Scientific 7023307 Steri-Cult... -
Page 45: Temp Controller Failure Temp Cntrl Error
If the tank not in use goes low, the warning light is activated. If both banks go low, the audible alarm and warning light are activated. 7023307 Steri-Cult Thermo Scientific... -
Page 46: Water Level Alarms
The door must be securely latched for heat and CO injection to resume after a door opening. If the door is latched, yet the display still shows “Door Ajar”, the door switch could be faulty. Call Technical Services. Thermo Scientific 7023307 Steri-Cult... - Page 47 Section 5 Alarms 7023307 Steri-Cult Thermo Scientific...
-
Page 48: Troubleshooting
Condensation on cabinet face Removable gasket not reinstalled properly. and the glass door. Condensation on cabinet interior RH out of calibration. Calibrate the RH, see Section 3. RH sensor not reinstalled correctly. Ensure RH sensor bottoms-out in hole. Thermo Scientific 7023307 Steri-Cult... -
Page 49: Preventive Maintenance
Perform Sterilization Cycle as needed. Inspect the inner and outer door latches and hinges for proper operation and excessive mechanical wear; inspect the line cord insulation and inner door silicone gasket for chips and cracks. Replace as required. 7023307 Steri-Cult Thermo Scientific... -
Page 50: Routine Maintenance
2. Wash the shelves and pilasters with disinfectant, then rinse with sterile water. Optional: The shelves and pilasters may be autoclaved. 3. Wash the inner door gasket with disinfectant. This gasket may be removed to be cleaned, or replaced. Thermo Scientific 7023307 Steri-Cult... -
Page 51: Cleaning The Humidity System
See Figure 7-1. Drain fitting 4. Install the hose barb end of this fitting to Figure 7-1. Water Bottle the supplied tubing and route to a drain or suitable 0.5 gallon (3.8 liter) container. 7023307 Steri-Cult Thermo Scientific... - Page 52 11. Also behind the access door is the humidifier. See Figure 7-5. Lift the latches on both sides of this small unit. 12. Separate the top and bottom sections. Carefully clean the inside of both sections and spray with 70% alcohol. Thermo Scientific 7023307 Steri-Cult...
- Page 53 8 seconds on, 2 seconds off continuous cycle. 8. Allow the incubator to run approximately 30 minutes to fill and agitate the solution in the humidity system. 9. Reconnect the drain fitting and drain the system again. 7023307 Steri-Cult Thermo Scientific...
-
Page 54: Flushing The Humidity System
Sterilization cycle. Caution The HEPA filter must be removed before the Sterilization cycle. The filtering material cannot withstand the 140°C heat. It will melt onto the shelves and be difficult to remove. Thermo Scientific 7023307 Steri-Cult... -
Page 55: Hepa Filter Maintenance
3. Install a new HEPA by first hooking the back edge of the filter as previously. 4. After the back edge is hooked, lift the front of the HEPA to the top of the chamber. Slide the filter bracket over the HEPA frame to secure it. Tighten the two wingnuts. 7023307 Steri-Cult Thermo Scientific... -
Page 56: Sterilization Cycle
The RH and CO2 sensors also need to be removed. • During the Sterilization Cycle, discoloration of some materials may occur. For example, stainless steel turns a straw color after a period of exposure to high temperatures. This is normal. Thermo Scientific 7023307 Steri-Cult... - Page 57 When the temperature cools down to the original set operating temperature or 30°C, whichever is higher, the display toggles between “CYCLE CANCELLED”, “REPLACE HEPAs”, “REPLACE SENSORS”, and “PRESS ENTER”. The green LED is lighted but no longer flashes. After the unit is powered back on with the CO sensor reinstalled, the display returns to “SYSTEM OK”. 7023307 Steri-Cult Thermo Scientific...
- Page 58 8. After ENTER is pressed with the sensor hole plugs installed, the Heat Phase initiates. The cycle initiation button flashes and the display toggles between “STERILIZING” and “HEAT PHASE”. During this period, the HEAT light will be on and the cabinet heats to sterilization temperature. Thermo Scientific 7023307 Steri-Cult...
- Page 59 “STERILIZING”. 10. After approximately 2 hours, a 5-second audible tone sounds, signaling that sterilization is complete. The Cool Phase begins. The display toggles between “STERILIZING” and “COOL PHASE”. Figure 8-4. Cool Phase in Sterilization Cycle 7023307 Steri-Cult Thermo Scientific...
- Page 60 Be sure the sensors reach the hole limit. Caution RH and CO sensors must be reinstalled correctly for systems to properly sense chamber conditions! If not, RH and CO levels may be uncontrolled. Thermo Scientific 7023307 Steri-Cult...
- Page 61 19. Verify the calibration of the sensors. See Section 3, CO or RH. Note The sterilization process can cause the inner door knob to become difficult to turn. A small amount of mineral oil, vegetable oil or similar oil applied to the knob should remedy this. 7023307 Steri-Cult Thermo Scientific...
-
Page 62: Factory Installed Options
Figure 9-1. Remote Alarm Outputs Note After connecting the incubator to the external alarm system, verify proper alarm operation by simply placing the incubator power switch to its off position to simulate a power outage condition. Thermo Scientific 7023307 Steri-Cult... -
Page 63: Rs485 Interface
(2) orange wires (1) red wire (1) green wire (1) black wire (3) yellow wires 1535 Junction to incubator RJ-11 connector Figure 9-3. RS485 Wiring CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge (ESD) 7023307 Steri-Cult Thermo Scientific... -
Page 64: Connecting The Analog Output Boards
191763 0-1 V 191762 0-5 V Output Scaling Output Scaling Output Scaling 4-20 mA Equals 0-1 V Equals 0-5 V Equals Temperature 12.5-162.5°C 0.0-150.0°C 0.0-150.0°C 0-100 %RH 0-100 %RH 0-100 %RH 0.0-100.0 %CO 0-100.0 %CO 0-100.0 %CO Thermo Scientific 7023307 Steri-Cult... - Page 65 ±1 unit. There is no calibration of the analog outputs by the incubator, so the instrument(s) connected to the analog outputs must be calibrated to match the incubator display before returning to service. 7023307 Steri-Cult Thermo Scientific...
-
Page 66: Co 2 Gas Guard
Inlet # 2. If the gas supply to Inlet #1 is replenished, the incubator will continue to operate using the gas supplied through Inlet #2 unless the operator changes the Tank Select from #2 to #1 through Configuration mode. Refer to Section 4, Configuration. Thermo Scientific 7023307 Steri-Cult... - Page 67 “Tank 1&2 Low”. The audible alarm continues to ring until the Silence key is pressed. The audible alarm rings back every 15 minutes after the alarm is silenced if the Gas Guard continues to detect that both gas supply pressures are below 10 psig (68.94 kPa). 7023307 Steri-Cult Thermo Scientific...
-
Page 68: Specifications
*Specifications are based on nominal voltages of 115 V or 230 V in ambients of 22°C to 25°C. **Humidity greater than or equal to 90% may lead to condensation depending on environmental conditions in the work area. Thermo Scientific 7023307 Steri-Cult 10-1... - Page 69 ....1/4” (0.64 cm) hose barbed Drain port ..3/8” (0.95 cm) barbed fitting with shutoff 10-2 7023307 Steri-Cult Thermo Scientific...
- Page 70 Net weight ..Model 3307/3308 - 330 lbs. (149.7 kg), . . Model 3310/3311 - 410 lbs. (186.0 kg) Shipping (motor)..Model 3307/3308 - 400 lbs. (181.4 kg), Model 3310/3311 - 480 lbs. (217.7 kg) Thermo Scientific 7023307 Steri-Cult 10-3...
- Page 71 Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. 10-4 7023307 Steri-Cult Thermo Scientific...
-
Page 72: Servicing
290174 RH sensor assembly, 1ft. 230180 12 A, DPDT circuit breaker switch (power) 400201 Switcher kit, 40 W 360213 Pressure switch (Gas Guard option) 250121 Solenoid gas valve (12 VDC Gas Guard option) Thermo Scientific 7023307 Steri-Cult 11-1... - Page 73 Water cleaning cell (for water reservoirs, pans, and bottles) 130101 Water cleaning cell (quantity of 6) 5300613 Filter/Stopper Assy (SMART-VUE) 430108A Auto-Lock power inlet cord with IEC320 female plug 400218 12 VDC Single Output Power Supply 11-2 7023307 Steri-Cult Thermo Scientific...
-
Page 74: Replacing The Fuses
RH air filters are recommended to be replaced annually. Follow the steps below to replace the filters. 1. Turn off the incubator’s power switch and disconnect the power cord. 2. Remove the four screws from the left side panel. Set the panel aside. Thermo Scientific 7023307 Steri-Cult 11-3... - Page 75 4. In the right side of the opening is the humidity system. Locate the RH air filter in the upper right corner (Figure 11-2). Disconnect from the tubing and replace. 5. Install the panel and secure with the screws. 6. Return the unit to service. 11-4 7023307 Steri-Cult Thermo Scientific...
- Page 76 Thermo Scientific 7023307 Steri-Cult 12-1...
- Page 77 Section 12 Warranty Information 12-2 7023307 Steri-Cult Thermo Scientific...
- Page 79 thermoscientific.com © 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. Thermo Fisher Scientific (Asheville) LLC 401 Millcreek Road Marietta, Ohio 45750...
Need help?
Do you have a question about the Steri-Cult 3307 Series and is the answer not in the manual?
Questions and answers