Pipetting Techniques; Forward Pipetting - Human HumaPette Smart-Line User Manual

pIpettIng technIqueS

5 PIPETTING TECHNIQUES

5.1 Forward pipetting

Make sure that the tip is firmly attached to the tip cone. For
best results the thumb button should be operated slowly and
smoothly at all times, particularly with viscous liquids.
Hold the HumaPette vertically during aspiration. Make sure
that the liquid and container vessel are clean and that the
HumaPette, tips and the liquid are at the same temperature.
1. Depress the operating button to the first stop.
2. Place the tip(s) just under the surface of the liquid
(2–3 mm) and smoothly release the operating button.
Carefully withdraw the tip from the liquid, touching it
against the edge of the container to remove the excess.
3. Liquid is dispensed by gently depressing the thumb button
to the first stop. After a short delay continue to depress
the thumb button to the second stop. This procedure will
empty the tip(s) and ensure accurate delivery.
4. Release the thumb button to the ready position. If neces-
sary change the tip(s) and continue with pipetting.
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