Human HumaCount 30TS Service Manual
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Human HumaCount 30TS Service Manual

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HumaCount 30
and 60
TS
TS
| Service Manual
16420/302

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  • Page 1 HumaCount 30 and 60 | Service Manual 16420/302...
  • Page 3 No part of this documentation may be reproduced in any form, nor processed, copied or distributed by means of electronic systems, without prior permission of Human GmbH in writing. Since all precautionary measures were taken into account in producing these operating instructions, the manufacturer accepts no responsibility for any errors or omissions.

  • Page 5: Table Of Contents

    CONTENTS TABLE OF CONTENTS 1 SAFETY INSTRUCTIONS 1.1 INTRODUCTION 1.2 USER WARRANTY 1.3 INTENDED USE OF THE INSTRUMENT 1.4 GENERAL SAFETY WARNINGS 1.5 DISPOSAL MANAGEMENT CONCEPT 1.6 INSTRUMENT DISINFECTION 1.7 BIOHAZARD WARNING 2 INTRODUCTION 2.1 NAME AND SERIAL NUMBER 2.2 INTEGRATED SOFTWARE 3 FUNCTIONAL DESCRIPTION 3.1 MAIN ELECTRONIC PARTS OF THE ANALYZERS 3.1.1 Counting chamber with electrodes and measuring aperture...
  • Page 6 4.2 ASSEMBLED ANALYZER 4.2.1 Front Panel 4.2.2 Side Panel 4.2.3 Rear Panel (HC30TS) 4.2.4 Construction Front 4.2.5 Construction Side 5 OPERATION OF THE FLUIDIC SYSTEM 5.1 INITIALIZATION OF THE FLUIDIC SYSTEM 5.2 OPERATION OF THE FLUIDIC SYSTEM IN HUMACOUNT 30 TS 5.2.1 Flow diagram of measurement 5.2.2 Sampling process 5.2.3 Needle washing process...
  • Page 7 CONTENTS 6.4 SERVICE CALIBRATION 6.5 SETTING RBC AMPLIFIER GAIN 6.6 SETTING WBC AMPLIFIER GAIN 7 CHECKIG THE PROPER OPERATION 7.1 SELF TEST 7.1.1 Self Test Screens 7.1.2 Normal range of Self Test parameters 7.1.3 Troubleshooting Guide for Self test 7.2 SERVICE MENU 7.2.1 Entering to Service Menu 7.2.2 Troubleshooting 7.2.3 Stress...
  • Page 8 10.3 CABLING DIAGRAM 10.4 TUBING SCHEMATICS 10.5 RECOMMENDED KIT OF TOOLS...

  • Page 9: Safety Instructions

    HUMAN, not regularly maintained, used with equipment not approved by HUMAN or used for purposes for which it was not designed.

  • Page 10: Intended Use Of The Instrument

    Use only the fuse type and rating specified by the manufacturer for this instru- ment, use of fuses with improper ratings may pose electrical and fire hazards. To avoid fire or shock hazard, observe all ratings and markings on the instrument. HumaCount 30TS / 60TS | Service manual...

  • Page 11: Disposal Management Concept

    1.6 Instrument Disinfection Analytical instruments for in vitro diagnostic involve the handling of human samples and controls which should be considered at least potentially infectious. Therefore every part and accessory of the respective instrument which may have come into contact with such samples must equally be considered as potentially infectious.

  • Page 12: Biohazard Warning

    1.7 Biohazard warning Analytical instruments for in vitro diagnostic application involve the handling of human samples and controls which should be considered at least potentially infectious. Therefore every part and accessory of the respective instrument which may have come into contact with such samples must equally be considered as potentially infectious.
  • Page 13 SAFETY INSTRUCTIONS Notes:...
  • Page 14 HumaCount 30TS / 60TS | Service manual...

  • Page 15: Introduction

    The software version number can be read out from the Device Information or from the Self test submenu. Every HC30TS / HC60TS software version is upgradeable (using an USB flash drive) by the latest program developed by HUMAN, and it can be downloaded from: http://www.human.de...
  • Page 16 HumaCount 30TS / 60TS | Service manual...

  • Page 17: Functional Description

    FUNCTIONAL DESCRIPTION 3 FUNCTIONAL DESCRIPTION 3.1 Main electronic parts of the analyzers HC30 / HC60 contain the following electronic parts: 1. Counting chamber (2 pcs in HC60TS) with electrodes and measuring aperture 2. HGB Measuring Head 3. Cell Counter Amplifier Board 4.
  • Page 18 FIGURE 2 Block diagram HumaCount 30TS / 60TS | Service manual...

  • Page 19: Counting Chamber With Electrodes And Measuring Aperture

    FUNCTIONAL DESCRIPTION 3.1.1 COUNTING CHAMBER WITH ELECTRODES AND MEASURING APERTURE Impedance method is used for determination of volume and number of cells. In this method a known volume of dilution is drawn through a small aperture. Constant current is passed through the aperture from one side to the other. When a cell passes through the aperture, it causes a change in resistance, which generates a voltage pulse.

  • Page 20: Hgb Measuring Head

    HSW signal and isolate the input of the amplifier. Test circuit makes possible to generate test pulses (with TEST and PLS signals through FETs) for checking the proper operation of the amplifier channel. HumaCount 30TS / 60TS | Service manual...

  • Page 21: Cell Counter Amplifier Board - Hc60Ts

    FUNCTIONAL DESCRIPTION FIGURE 6 Cell counter amplifier board 1 Connection to HVB on Main 2 Connection to CSA1 on Main 3 Connection to DIGIO on Main Amplifier board includes a 3-stage main amplifier channel, which gains input signal to the 0...3.3 V range (this is the input range of the A/D converter, which is placed on the MAIN board).
  • Page 22 DHON signal - from the MAIN board - switches on (with Q4) the LED and the PLS signal switches off the Photo Detector in the HGB head, to prevent noise generated by the HGB detector. HumaCount 30TS / 60TS | Service manual...

  • Page 23: Main Cpu Board

    FUNCTIONAL DESCRIPTION 3.1.5 MAIN CPU BOARD This board contains: DIMM-PC and measurement processing unit, 4 motor controllers, valve & pneumatic controller/drivers, pump driver(s) power supply for internal printer (+7.5V) and digital circuitry (+5V, +3.3V) MAIN board is responsible to control the instrument: contains the main power regulator circuits, valve and motor driver circuits and other connections for the fluidic and pneumatic system’s parts, responsible for the specific measurement processing functions.
  • Page 24 >5VDC, 3.3VDC 9 High Voltage Circuit 12VDC -> ±12VDC, 50VDC for measurement, 150VDC for cleaning 10 Microcontroller 11 TFT connection 12 TFT backlight driver 13 Motor drivers 14 Valve drivers 15 Pump drivers HumaCount 30TS / 60TS | Service manual...
  • Page 25 FUNCTIONAL DESCRIPTION FIGURE 10 Main board - rear view 1 Pressure Sensor 2 Motor Connectors 3 Front Panel USB Connector 4 Connector to TFT, Backlight and Start Button 5 Connectors to Amplifiers 6 Internal battery 7 Power Connector 8 Power Switch Connector 9 USB B Type Interface 10 USB A Type Interfaces 11 Connectors to Optos...

  • Page 26: Dimm-Pc* Module

    10 SSD 3.1.7 OPTO SENSORS Opto sensor snap-in modules are responsible for checking motor positions. There are 6 opto sensors in HC60TS, and 5 in HC30TS (see cabling diagram). FIGURE 12 Opto sensor HumaCount 30TS / 60TS | Service manual...

  • Page 27: Valve Boards

    FUNCTIONAL DESCRIPTION 3.1.8 VALVE BOARDS There are two kinds of valve boards: Valve board 1-5 and Valve board 6-12. FIGURE 13 Valve assembly 1 Valves 2 Valve Board 3 Connection to Main board HC30TS has 5 valves, while HC60TS has 6 valves in Valve board 6-12 module. The valve boards are connected to controller and driver chips which are located on the MAIN board.

  • Page 28: External Power Supply

    The input socket of the power supply is a standard 3-terminal plug, with power cable connection; the output is a coaxial power connector. FIGURE 15 Power supply 1 230 AC inlet 2 12V DC outlet HumaCount 30TS / 60TS | Service manual...

  • Page 29: Main Mechanic And Fluidic Parts

    MAIN MECHANIC AND FLUIDIC PARTS 4 MAIN MECHANIC AND FLUIDIC PARTS HC30TS and HC60TS Hematology Analyzers consist of the following mechanic and fluidic parts: 1. Sample rotor 2. Sampling needle 3. Washing head 4. Sample/Horizontal moving unit 5. Micro Dilutor 6.

  • Page 30: Single Parts

    The Sample rotor unit uses a stepper motor, connected to the MAIN board directly. The rotor has opto sensors for positioning. FIGURE 18 Sample probe motors 1 Vertical motor 2 Sample/Horizontal motor (not visible) HumaCount 30TS / 60TS | Service manual...

  • Page 31: Sampling Needle

    MAIN MECHANIC AND FLUIDIC PARTS 4.1.2 SAMPLING NEEDLE Sampling needle is assembled in the H&V moving unit and it makes the sample aspirations. Correct setting of sampling needle is necessary and very important (see Chapter Adjustments). 4.1.3 NEEDLE WASHING HEAD Washing head is located at the bottom of the H&V moving unit and it is for Clean or replace washing head cleaning the outer surface of the sampling needle.

  • Page 32: Dilutor Block - Hc30Ts

    (cogwheel and cogged bar) should be done regularly using machine grease. It is recommended to check and repeat greasing of piston tips, and transmission gear every year, or after 10000 measurements. HumaCount 30TS / 60TS | Service manual...

  • Page 33: Dilutor Block - Hc60Ts

    MAIN MECHANIC AND FLUIDIC PARTS 4.1.6 DILUTOR BLOCK – HC60TS FIGURE 22 1 Micro dilutor 2 Diluent 1 syringe 3 Positioners 4 Positioners 5 Lyse syringe 6 Diluent 2 syringes In HC60TS this unit includes two dilutor channels – one for diluent and sampling, and another one for lyse and diluent reagents.

  • Page 34: Measuring Block (Hc30Ts)

    The measuring block contains all components, counting chamber, measuring tubes, HGB head, draining tubes. FIGURE 23 4.1.8 MEASURING BLOCK (HC60TS) The measuring block contains all components, counting chambers, measuring tubes, HGB head, draining tubes. FIGURE 24 HumaCount 30TS / 60TS | Service manual...

  • Page 35: Pump

    MAIN MECHANIC AND FLUIDIC PARTS 4.1.9 PUMP Pump generates regulated vacuum and drains the fluidic system. It is connected to the MAIN board and it has its own driver circuit (Power FET). In HC60TS there are two pumps. The pump is maintenance free. FIGURE 25 4.2 Assembled Analyzer 4.2.1 FRONT PANEL...

  • Page 36: Side Panel

    2 Reagent inlets 3 cover screw 4 Power switch 5 Cover screw 6 USB A inlet 7 USB B inlet 8 Cover Screw 9 Grounding screw 10 12 VDC power IN 11 Cover screw HumaCount 30TS / 60TS | Service manual...

  • Page 37: Construction Front

    MAIN MECHANIC AND FLUIDIC PARTS 4.2.4 CONSTRUCTION FRONT FIGURE 29 1 Main board 2 Pump II (only in HC60TS) 3 Vertical motor 4 Pump I 5 Amplifier box 6 Valves 7 Dilutor motor 8 Valves 9 Sample/Horizontal motor 4.2.5 CONSTRUCTION SIDE FIGURE 30 1 Valves 1-5 2 Micro dilutor...
  • Page 38 FIGURE 31 Construction side of HC30TS 1 MAIN board 2 Dimm-PC FIGURE 32 Construction side of HC60TS 1 MAIN board 2 Dimm-PC HumaCount 30TS / 60TS | Service manual...

  • Page 39: Operation Of The Fluidic System

    OPERATION OF THE FLUIDIC SYSTEM 5 OPERATION OF THE FLUIDIC SYSTEM This section describes the main fluidic steps of HC30TS / HC60 TS measurement cycle. The instrument’s Fluidic Schematics are shown in Section 10.4 of this manual. The following figures show total measurement flow diagram and detailed descriptions of processes for understanding the fluidic system work.

  • Page 40: Operation Of The Fluidic System In Humacount 30 Ts

    5.2.1 FLOW DIAGRAM OF MEASUREMENT FIGURE 33 Chamber draining Fill up with 1ml diluent for WBC dilution Sampling process Needle washing Diluting process (1:160) Sampling process (from primary dilution) Needle washing Lysing process HumaCount 30TS / 60TS | Service manual...
  • Page 41 OPERATION OF THE FLUIDIC SYSTEM FIGURE 34 Generating Fill up with 1ml measuring diluent for RBC vacuum dilution WBC counting process Dilution process (1:25600) measurement Needle washing Extensive cleaning Generating measuring vacuum Blank HGB measurement measurement Draining...

  • Page 42: Sampling Process

    There is also another sampling process for the second (RBC) dilution, 25 μL of primary dilution is aspirated by the sampling needle from the chamber but it is kept in the sampling needle during the WBC measurement and the cleaning process. HumaCount 30TS / 60TS | Service manual...

  • Page 43: Needle Washing Process

    OPERATION OF THE FLUIDIC SYSTEM 5.2.3 NEEDLE WASHING PROCESS Both instruments clean the sampling needle with diluent in the washing head after sampling. It is important to clean the outer surface of the sampling needle to avoid inaccurate sampling. The Macro syringe doses and the pump drains the diluent from the washing head, while sampling needle moves upwards so that the total length of it is washed and cleaned.

  • Page 44: Diluting Process

    The second sample – 25 μL of primary dilution – is stored in the sampling needle during the WBC measurement and the cleaning process. The instrument makes the second (RBC) dilution into the chamber after these processes. HumaCount 30TS / 60TS | Service manual...

  • Page 45: Lysing Process

    OPERATION OF THE FLUIDIC SYSTEM 5.2.5 LYSING PROCESS In this step the set lysing reagent is added into the measuring chamber through V5 (On), while the Lyse syringe moves upwards. This process makes the WBC/ HGB dilution with lyse reagent. FIGURE 38 V2 DilWash V4 DilNeedle...

  • Page 46: Counting Process

    WASTE 1-3 = Off Open = On 2-3 = On For noise prevention there is no mechanical or electronic activity during the counting process and the door should be closed for better shielding. HumaCount 30TS / 60TS | Service manual...

  • Page 47: Chamber Draining Process

    OPERATION OF THE FLUIDIC SYSTEM 5.2.7 CHAMBER DRAINING PROCESS Chamber draining is made under pressure control. Pump drains chamber while puffer reservoir and thus the pressure sensor is connected to the draining tube. The instrument can detect the empty state of the chamber from drop of vacuum FIGURE 40 V2 DilWash V4 DilNeedle...

  • Page 48: Operation Of The Fluidic System In Hc60Ts

    (1:160) (1:160) Sampling process (from primary dilution) Lysing process Needle washing Dilution process Dilution process (1:23000) (1:23000) Generating measuring vacuum WBC counting process, HGB measurement RBC counting process Draining Cleaning Fast Blank measurement HumaCount 30TS / 60TS | Service manual...

  • Page 49: Sampling Process

    OPERATION OF THE FLUIDIC SYSTEM 5.3.1 SAMPLING PROCESS The aspirating needle aspirates 25 μL (50 μL in prediluted mode) of blood sam- ple. The Micro dilutor syringe makes the aspirating while the M3 Micro-dilutor motor moves down. The syringes are mechanically connected with a loose me- chanism, so there is a phase along the track, where the micro dilutor doesn’t move.

  • Page 50: Diluting Process

    Open = On 2-3 = On WASTE The second sample – 25 μL of primary dilution – is made right after the first dilution. The second dilution is made in the RBC chamber. HumaCount 30TS / 60TS | Service manual...

  • Page 51: Lysing Process

    OPERATION OF THE FLUIDIC SYSTEM 5.3.3 LYSING PROCESS In this step the set lysing reagent is added into the measuring chamber through V10 (On), while the Lyse syringe moves upwards. This process makes the WBC/ HGB dilution with lyse reagent. FIGURE 44 V2 DilNeedle V1 DilWash...

  • Page 52: Counting Process

    1-3 = Off Open = On 2-3 = On WASTE For noise prevention there is no mechanical or electronic activity during the counting process and the door should be closed for better shielding. HumaCount 30TS / 60TS | Service manual...

  • Page 53: Chamber Draining Process

    OPERATION OF THE FLUIDIC SYSTEM 5.3.5 CHAMBER DRAINING PROCESS Chamber draining is made under pressure control. Pump drains chamber while puffer reservoir and thus the pressure sensor is connected to the draining tube. The instrument can detect the empty state of the chamber from drop of vacuum. FIGURE 46 V2 DilNeedle V1 DilWash...

  • Page 54: Cleaning Process

    After that the Diluent 2 syringes pushes the cleaner reagent remaining in the tube between V10 and V3 into the chamber. 5.3.7 SHUTDOWN PROCESS The fluidic shutdown performs a fluidic system cleaning with cleaner. HumaCount 30TS / 60TS | Service manual...

  • Page 55: Adjustments

    ADJUSTMENTS 6 ADJUSTMENTS The adjustments below are made in the factory. Readjustment of following parts is necessary if some components are replaced. 6.1 Common adjustments 6.1.1 VERTICAL MOVEMENT, SETTING TIMING BELT TENSION The timing belt tension could be set with positioning the vertical motor using the oval holes in the mounting plate.

  • Page 56: Setting The Needle Shaft

    FIGURE 50 6.1.3 SETTING THE NEEDLE SHAFT The needle shaft must be fastened. If it was loose it could be adjusted with the set screw on top end of the shaft. FIGURE 51 HumaCount 30TS / 60TS | Service manual...

  • Page 57: Setting The Position Of The Chambers

    ADJUSTMENTS 6.1.4 SETTING THE POSITION OF THE CHAMBERS After setting the needle position according to chapter 5.2.2 and 5.3.1. the horizontal position of chamber bracket should be checked. The needle must not go down exactly in the center of the chamber. Chamber bracket can be moved left or right if necessary.

  • Page 58: Hc30Ts Specific Adjustments

    The setting of the horizontal movement is correct, when the opto wheel is in the position shown in the picture, then the aspirating needle bracket is as close to the cantilever as possible. FIGURE 54 HumaCount 30TS / 60TS | Service manual...

  • Page 59: Hc60Ts Specific Adjustments

    ADJUSTMENTS 6.3 HC60TS specific adjustments 6.3.1 SETTING THE DILUTOR MECHANICS The micro-dilutor’s movement must be set by the following procedure: 1. Push the dilutor pistons up as possible. 2. Fasten the set screw of the upper fixing ring. 3. Fasten the lower fixing ring’s set screw in the position shown in picture on the left.

  • Page 60: Service Calibration

    Press white data field to modify calibration factor. A numeric input screen will show up so that you can enter values. All values must be in the 0.8…1.2 range. Press Accept to proceed with new settings, or Cancel to keep values unchanged. HumaCount 30TS / 60TS | Service manual...

  • Page 61: Setting Rbc Amplifier Gain

    ADJUSTMENTS History: You can check the previous calibration factors with the date of change in a table form. Apply user calibration factors function is used to combine user and service calibration factors. The software will multiply the existing factors, and move them to the Service level to set user factors to 1.00.

  • Page 62: Setting Wbc Amplifier Gain

    WBC measurement could be increased or decreased by approx. ±10%. Please be advised that changing this value requires to recalibrate the device to get proper results! The settings are under Settings-->Measurement-->Settings-->Calibration. HumaCount 30TS / 60TS | Service manual...

  • Page 63: Checkig The Proper Operation

    CHECKIG THE PROPER OPERATION 7 CHECKIG THE PROPER OPERATION 7.1 Self Test There is a built-in Self test and Service menu in each model. Self test can be used to check the operation of the instrument. The test results can be printed or saved to USB flash disk. With the Retry button the self test is repeated.

  • Page 64: Normal Range Of Self Test Parameters

    Fault on Amplifier Offset tiometer Amplifier HIGH board board Amplifier Boards is not Check cables and connected to main connectors coming from board the Amplifier Amplifier test HIGH Instrument not grounded Check mains ground lead HumaCount 30TS / 60TS | Service manual...

  • Page 65: Service Menu

    CHECKIG THE PROPER OPERATION Check current generator, LOW or Peak of pulses Fault on Amplifier board and test generator FET on HIGH Amplifier board Instrument not Dev. (noise) HIGH Check mains ground lead grounded Instrument not Noise HIGH Check mains ground lead grounded Vacuum Peristaltic pump failure...

  • Page 66: Troubleshooting

    7.2.2 TROUBLESHOOTING With Troubleshooting options provide tools to test mechanical components. From the Motor Test submenu the service person could run each or all motor tests. Optosensor’s states can be checked in this screen. HumaCount 30TS / 60TS | Service manual...

  • Page 67: Stress

    CHECKIG THE PROPER OPERATION Pressing the 0 or 1 buttons the valves could be toggled. 1 stands for electronically forced state. Pressing the Pump 0 or 1 button the peristaltic pump could be switched on/off. The current vacuum in the puffer reservoir is displayed under the buttons. You can check the offset on the amplifier board.

  • Page 68: Needle Position Check

    Certain service functions are accessible only for the SERVICE user. In Main menu touch Exit button. If you are logged in as different user touch Logout(USERNAME) and then the Log In button, else touch Log In button. HumaCount 30TS / 60TS | Service manual...
  • Page 69 CHECKIG THE PROPER OPERATION At Log In screen Press the START button on front panel, so the Login Name will change to Service. Touch Password empty field. Type in the Service User password which is 6484, then touch Enter button. Touch Accept button on Log In screen.
  • Page 70 HumaCount 30TS / 60TS | Service manual...

  • Page 71: Service Operation

    SERVICE OPERATION 8 SERVICE OPERATION 8.1 Possible Causes of Noise Generally high count of any particle - even if you think it should be low, or near zero - can be caused by noise, i.e. something interfere with the measurement. The most important thing in these cases is to identify the source of the noise, otherwise you cannot protect the system against it.

  • Page 72: External Electrical Noise

    Replacement of chamber is required. Contaminated draining tube starts to conduct due to protein or lipid build- up. It is very easy to identify this case. After replacing the drain tube of the HumaCount 30TS / 60TS | Service manual...
  • Page 73 SERVICE OPERATION measuring chamber (mainly WBC), WBC histogram peak, or PLT becomes low soon. Normally a good cleaner is required to dissolve lipid or protein build-up. Sometimes the cleaner is not strong enough to keep this tube clean enough. Periodic washing using 1% hand warm bleach solution helps. 8.1.4.2 Bad insulation of electronic signal paths: In these cases check for any capacitive coupling of electronic signals to the chamber:...
  • Page 74 Lyse path guides in noise (during counting, if the a liquid in the draining tube is touching lyse reagent in T-fitting, noise can appear). Check the lyse path, and the lyse valve as well. HumaCount 30TS / 60TS | Service manual...

  • Page 75: Maintenance

    MAINTENANCE 9 MAINTENANCE 9.1 Weekly User Maintenance Perform weekly maintenance before turning on the power switch. The right side has a side door giving access to the fluidic system and the mechanical parts easily. 9.1.1 CLEANING NEEDLE WASHING HEAD Needle washing head cleans the outer surface of the aspirating needle with di- luent.

  • Page 76: Periodic Maintenance By Service

    The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease. Apply just a thin layer, and move it along the perimeter of the piston, so that some of the material goes into the gaps between the sealing rings. HumaCount 30TS / 60TS | Service manual...

  • Page 77: Checking And Replacing Washing Head

    MAINTENANCE Repeat this step for lyse and dilutor pistons as well. Check the condition of the micro piston sealing, and replace if necessary. 9.2.4 CHECKING AND REPLACING WASHING HEAD Check the state of the washing head, and replace if necessary. After replacing washing head, do not forget to perform correct adjustment of sampling needle height (see Section 4.1.2).
  • Page 78 HumaCount 30TS / 60TS | Service manual...

  • Page 79: Appendices

    APPENDICES 10 APPENDICES 10.1 Warning flags Analyzer SW displays warning flags for each individual measurement to notify user about status of results. The following table summarizes warning flags and gives explanation of their possible cause and a few hints to overcome the problem.

  • Page 80: Usb B Connector Communication

    RBC blank 10.2 USB B connector communication The byte stream is a human readable ASCII character stream, with occasional control characters. Most programming environments are able to handle this stream as a simple ASCII string or text. The stream is line-oriented with special characters to separate fields.

  • Page 81: Characters And Basic Structure

    APPENDICES 10.2.1 CHARACTERS AND BASIC STRUCTURE The byte stream uses the ASCII characters in the range 1..255 (http://en.wikipedia. org/Ascii ), or 0x01..0xFF in hexadecimal. A record transmission consists of three parts: a small header, a big text body, and a small footer. A single record is never longer than 8192 bytes. A transmission always starts with the control character „Start of Header”...
  • Page 82 „a” to „z” always the same, indicates the beginning of the WBC histo- WBC graph gram wbcscale is maximum 3 digit number, indicating the fl value Scale(fl):<HT>wbcscale of the last channel, value is usually 400 HumaCount 30TS / 60TS | Service manual...
  • Page 83 APPENDICES wbcchannels is the number of channels (columns) in the Channels:<HT>wbcchannels histogram, always 256 WMarker1:<HT>wm1 wm1 is the first WBC discriminator channel (RBC/WBC) WMarker2:<HT>wm2 wm2 is the second WBC discriminator channel (LYM/MON) WMarker3:<HT>wm3 wm3 is the third WBC discriminator channel (MON/NEU) chxx is the histogram height at a given channel (range Points:<HT>ch0<HT>...<HT>ch255 0..255), there are always wbcchannels values here (usually...
  • Page 84 10.3 Cabling diagram FIGURE 59 HC30TS cabling diagram HumaCount 30TS / 60TS | Service manual...
  • Page 85 APPENDICES FIGURE 60 HC60 cabling scheme...
  • Page 86 10.4 Tubing schematics FIGURE 61 MAIN Board Pressure Tubing schematics HC30TS Sensor PUFFER Dilutor Pump HumaCount 30TS / 60TS | Service manual...
  • Page 87 APPENDICES MAIN Board FIGURE 62 V 12 Pressure Tubing schematics HC60TS Sensor PUFFER Pump Pump...
  • Page 88 10.5 Recommended kit of tools Screwdrivers: • Cross Slot Screwdrivers (Philips) • Slot Screwdrivers • Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes) Pocket digital multimeter Diagonal Cutter (plier) Nipper HumaCount 30TS / 60TS | Service manual...
  • Page 89 APPENDICES...
  • Page 90 HUMAN Gesellschaft für Biochemica und Diagnostica mbH Max-Planck-Ring 21 • 65205 Wiesbaden • Germany Tel.: +49 6122/9988 0 • Fax: +49 6122/9988 100 eMail: human@human.de • www.human.de...

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Humacount 60ts

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