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Dhyana 95 V2
User Manual
V1.0.0
Copyright 2011-2024 Tucsen Photonics Co., Ltd.
All rights reserved.
Technical Support Email: service@tucsen.com
TEL: +86-591-28055080-818
Fax: +86-591-28055080-826

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Summary of Contents for Tucsen Dhyana 95 V2

  • Page 1 Dhyana 95 V2 User Manual V1.0.0 Copyright 2011-2024 Tucsen Photonics Co., Ltd. All rights reserved. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 2: Table Of Contents

    3.16.2. Software Trigger Mode ........................26 3.16.3. Hardware Trigger Mode ........................26 3.17. Trigger Output ............................32 3.17.1. Hardware Trigger output circuit ...................... 32 3.17.2. Trigger Output Timing Diagram ....................... 32 3.18. Cooling ..............................34 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 3 Appendix 3 : Third party software applications ....................58 Appendix 4 : Third party certification ......................59 Appendix 5 : Dew point table ......................... 60 Appendix 6 : Update log ..........................61 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 4: Introduction

    In any case, TUCSEN shall not be liable for any losses or damages arising from or related to the unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or other reasons, whether caused by infringement or other reasons.
  • Page 5 3) This document may contain technical or printing errors. Under no circumstances shall TUCSEN be liable for any loss or damage caused by unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or otherwise.
  • Page 6: Safety And Warning Information

    ⚫ Please ensure that there is at least 10 cm of space around the device ventilation opening to ensure airflow flow. Do not block the ventilation openings of the equipment during use, otherwise it may cause internal temperature to be too high and damage the equipment. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 7 ⚫ If the voltage applied to the camera is greater than or less than the nominal voltage of the camera, the camera may be damaged or malfunction. Caution ⚫ Please refer to the specification table for the nominal voltage of the camera. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 8: Product Specifications

    2. Product specifications 2.1. Packaging List Standard Item Name Specification Quantity Image sCMOS scientific grade Dhyana 95 V2 camera Power adapter 12V/8A Power adapter power cord Three core 250V/2.5A USB3.0 data cable USB flash drive Software and Drivers Water pipes...
  • Page 9: Quantum Efficiency Curve

    Optional Item Name Specification Quantity Image CameraLink frame grabber Firebird Frame grabber cable Level conversion box Standard 2.2. Quantum efficiency curve Figure 2-1 Quantum efficiency curve of Dhyana 95 V2 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 10: Window Curve

    2.3. Window curve Figure 2-2 Transmission curve of a standard glass window 2.4. Quartz window curve Figure 2-3 Transmission curve of a quartz window Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 11: Camera Power And Signal Connection

    If the software has been shut down but the camera light remains orange, it indicates that the camera has not been fully released and needs to be completely exited from the camera thread or be restarted before the indicator light will return to red. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 12: Features And Functions

    Coupled with large 11 μm x 11 μm pixels, it ensures ultra-high sensitivity. Additionally, the Dhyana 95 V2 has a readout noise of only 1.6 electrons and a full well capacity of approximately 100,000 electrons. This gives it a high dynamic range, enabling it to measure both strong and weak signals simultaneously, making it ideal for use in complex light intensity environments.
  • Page 13: Shutter Mode

    The difference in exposure time points between adjacent rows is also known as the line time (T line Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 14: Front-Illuminated And Back-Illuminated Scmos Technology

    The 95 V2 camera adopts a back-illuminated chip, achieving a peak QE of around 95%. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 15: Readout Noise

    RMS— median values are generally lower than RMS values. Figure 3-5 Readout noise distribution of an typical sCMOS camera Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 16: Defective Pixel Correction (Dpc)

    It is not recommended to use the DPC function for these applications or to use only the weakest correction level. The Dhyana 95 V2 adopts dynamic defective pixel correction, correcting using a 3x3 matrix of pixels. Currently, four correction levels are available, each corresponding to different thresholds, thereby controlling the intensity of defective pixel correction.
  • Page 17: Photo Response Non-Uniformity (Prnu)

    Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 18: Gain Mode

    (HG/LG) mode under HDR, High Gain/Low Gain_High Speed (HG/LG_HS) mode. Each mode has differences in synthesis principles, line times, gain values, and readout noise. Selecting the appropriate mode based on the actual scenario is essential to obtain high-quality imaging results. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 19: High Dynamic Range

    Combining high and low gain images through algorithms generates an HDR image. This mode is suitable for applications with large variations in signal strength. Figure 3-8 Schematic diagram of HDR mode Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 20: High Speed

    The software sets preset subregions and also supports manual settings, where the row window must be a multiple of 4, and the column window must be a multiple of 8. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 21: Binning Readout

    Table 3-2 Typical ROI area measured frame rate table for Dhyana 95 V2 camera under USB 3.0/ CameraLink USB3.0 (fps) CameraLink (fps) Column( HighGain_HS HighGain_HS/ Pixel) (Pixel) HDR/HG/LG HDR/HG/LG LowGain_HS /LowGain_HS 2048 2048 2048 1024 2048 2048 2048 2048 1391...
  • Page 22: Timestamp

    The camera accurately reads the start time of each frame with a time precision of 1 μs. In Mosaic V3 software, images are saved in .sen format, and timestamp information is displayed in the image information, supporting the export of image information to .csv format files. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 23: Frame Rate Calculation

    95 V2 are shown in the following table. Table 3-3 Line time ) Gain mode Readout Time(T readout (T ) line HDR/HG/LG 20.52 μs 2048*20.52 μs=42.02496 ms HighGain_HS/LowGain_HS 20.52 μs 2048/2*20.52 μs=21.01248 ms Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 24: Frame Rate Adjustment

    Hn: The number of rows selected horizontally; Vn: The number of rows selected vertically; : Line time; line : The set exposure time. Table 3-4 Calculation of frame rate for Dhyana 95 V2 camera Line time Horizontal Vertiacal Frame rate...
  • Page 25: Incident Photon Calculation

    Live mode is suitable for real-time preview, providing data stream output. Images are continuously output like a flowing stream. In this mode, users can freely modify settings such as exposure time, gain mode, region of interest, etc., for real-time preview and image capture Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 26 Figure 3-16 In the acquisition module, users can set the save path, file name, total number of frames and other information, and the image can be taken after the setting is completed. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 27: Software Trigger Mode

    Figure 3-18 3.16.3. Hardware Trigger Mode Hardware trigger mode waits for an external level trigger signal command to capture an image. It includes three modes: Standard Overlap, Standard Non-Overlap, and Synchronization. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 28 To suppress interference in the external trigger signal, external trigger filtering supports high and low-level filtering. This function filters out spikes in the level signal. The filtering time range can be set to [0, 1000000] μs, with the default being 0. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 29 , within the range of two line times. If the Trigger Filter idelay logic function is used, a Trigger Filter Delay Time T is added to this. filter Figure 3-22 Schematic of trigger delay Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 30 Standard (Non-Overlap) Trigger Mode: In this mode, when the camera is in live mode, it responds to the external trigger signal only after the readout ends. Figure 3-24 Standard (Non-Overlap) Trigger timing Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 31 (i.e., the reciprocal of the frame rate) to ensure that a frame of image is complete and error-free. Figure 3-26 Edge Trigger timing diagram Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 32 Dhyana 95 V2 camera supports single-trigger multi-frame acquisition function, you can modify the number of triggering out of the picture by setting the frame/signal in the standard triggering mode (refer to Figure 3-19), for example, by setting the number of 2, the triggering...
  • Page 33: Trigger Output

    The camera has three external trigger output interfaces, each independently capable of outputting six timing signals. Each output signal can be independently configured on the three output ports and can simultaneously output to different devices. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 34 (valid when the exposure time is larger than the readout time), the default pulse width is 5ms, which is customizable. And the output is in the TRIG.OUT2 interface by default. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 35: Cooling

    For complete low-vibration performance, the fan of the Dhyana 95 V2 camera can be completely turned off in the software, and a water cooling port is provided (refer to section 4.3 for installing water cooling).
  • Page 36: Installation

    4.2. Camera installation Figure 4-1 Dhyana 95 V2 camera connection diagram ① Dhyana 95 V2 camera ② Power adapter ③ USB 3.0 data cable ④...
  • Page 37: Water Cooling Pipe Installation

    (1) Place the camera on a stable workbench; (2) Connect the water cooling pipe to the water pipe joint on the camera and make sure it is inserted in place, as shown in Figure 4-2 below; Figure 4-2 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 38: Software Fan Status

    (2) Following the instructions for the recirculating water chiller, unplug the water hose from the chiller and drain the water from the chiller; (3) Press the adapter valve sleeve and remove the water hose from the recirculating water chiller to drain the internal water; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 39 7) After operation, turn off the power to the camera and chiller, and drain the water from both the chiller and the camera. Warning: 1) Do not turn off the recirculating water chiller during camera operation, as this may Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 40: Frame Grabber Installation

    1) When installing and disassembling the Frame grabber, be sure to operate with power off; 2) When connecting the CamraLink cable, please operate with the camera power off and do not hot plug or unplug. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 41: Driver Installation And Uninstallation

    (4) Selecting the contents of the installation, by default check the box to install Microsoft runtime libraries vcredist_2008 and vcredist_2013, unchecking the box may result in the software or third-party plug-ins not working; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 42 Figure 4-8 (5) Waiting for the driver installation to be completed; Figure 4-9 (6) Click "Finish" to complete the driver installation; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 43: Cameralink Driver Installation

    (1) The camera is connected to the installed CameraLink Frame grabber CameraLink1 and CameraLink2 interfaces on the computer host through two CameraLink cables, and the interface sequence needs to be one-to-one corresponding; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 44 CameraLink Frame grabber will appear in the device manager and display FireBird Baseboard. After connecting the camera, it will occupy the COM port, as shown in the following figure. Figure 4-13 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 45: Cameralink Driver Uninstallation

    Operation steps: (1) Open the supporting USB drive, double-click to run Mosaic V3 software; (2) Select the installation path, default to C drive, users can customize the installation path according to your needs; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 46 (3) Select the installation content. By default, select the option to install drivers and Microsoft runtime library vc-redist_2015, canceling the installation can result in the camera not being recognized by the software; Figure 4-17 (4) Configure installation parameters and select whether to generate desktop shortcuts; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 47 Figure 4-18 (5) After confirming all installation parameters, click "Install" to start executing the installation action; Figure 4-19 (6) Waiting for installation to complete; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 48: Uninstallation

    C drive path will take effect; Figure 4-21 Uninstalling through installation package (2) Under the installation path, find unins000.exe to uninstall, double-click uninstall; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 49 Figure 4-22 (3) Uninstall from the computer program management interface; Note: After uninstalling and reinstalling the software, all software configuration information and calibration table data will be deleted. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 50: Maintenance

    1) Priority is given to the use of air blowing, the use of ear washing ball or air blowing off the general dust; together with the brush can remove most of the dust; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 51 2) If the dirt still exists after following the above steps, try wiping again by following the above steps. If this does not solve the problem, consider that the chip is internally dirty. Please be sure to contact us at this time. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 52: Troubleshooting

    4) If water-cooling is used, make sure that the water-cooling cycle is functioning properly. 6.4. The frame rate cannot reach the nominal level 1) Confirm whether the exposure time affects the frame rate, and set the minimum exposure Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 53 5) If the frame rate cannot reach the nominal value using the CameraLink interface, it is necessary to check whether the bandwidth of the grabber card is limited/whether the recommended grabber card interface is used. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 54: Faqs

    If it is an ambient light source, switching off the illumination source is sufficient. If from an irradiated sample light source, a regulated light source is required for illumination. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 55: After-Sales Support

    3) Contact professionals for technical support: ⚫ TEL: +86-591-28055080-818 ⚫ Email: service@tucsen.com ⚫ Visiting the official website to leave a message: http://www.tucsen.com 4) Please prepare the following information in advance: ⚫ Camera model and S/N (product serial number); ⚫ Software version number and computer system information;...
  • Page 56: Appendix 1 : Dimensions

    Appendix 1 : Dimensions Unit: mm, Diameter: ø. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 57: Appendix 2 : Specifications

    Appendix 2 : Specifications Camera Model Dhyana 95 V2 Sensor Type BSI sCMOS Sensor Model Gpixel GSENSE400BSI Peak QE 95% @ 560 nm Color / Mono Mono Array Diagonal 31.9 mm Effective Area 22.5 mm x 22.5 mm Resolution 2048 (H) x 2048 (V) Pixel Size 11 μm x 11 μm...
  • Page 58 PCIe 2x4, supports 2GB/s transfer from motherboard to computer Transmission Speed 1.7 GB/s Format Half length PCI Express Note: The parameters in this table are typical values and are subject to change without notice. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 59: Appendix 3 : Third Party Software Applications

    Appendix 3 : Third party software applications Provide plugins for calling third-party software (LabVIEW, Matlab, Micro-Manager, etc.), please Tucsen camera software download - click on the link to download the configuration: Tucsen Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 60: Appendix 4 : Third Party Certification

    Appendix 4 : Third party certification Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 61: Appendix 5 : Dew Point Table

    20.3 22.3 10.5 13.9 16.7 19.1 21.3 23.2 11.4 14.8 17.6 20.1 22.3 24.2 12.3 15.7 18.6 21.1 23.3 25.2 13.2 16.6 19.5 22.0 24.2 26.2 14.0 17.5 20.4 23.0 25.2 27.2 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 62: Appendix 6 : Update Log

    Appendix 6 : Update log Version Date Modified Content V1.0.0 2024-2-26 Create this document. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

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