Electron capture detector for gc-2010 plus (70 pages)
Summary of Contents for Shimadzu SPD-M20A
Page 1
228-30772N PHOTODIODE ARRAY UV-Vis DETECTOR FOR SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH SPD-M20A SERVICE MANUAL CAUTION This service manual has been edited for those that have received service related training. Those that have not been trained, should not use this manual.
Page 4
< When LCsolution is installed > ................51 < When CLASS-VP is installed > ................51 < When PDA Utility software of SPD-M20A attachment is installed > ....52 < Select connect instrument > ................52 < Select System > ....................52 <...
Page 5
> ........................ 79 < Preparative Flow cell(fixed path length,0.5mm) > ..........79 < High Sensitivity UHPLC Optional Flow cell > ............ 80 < Mechanical parts list > ..................81 < Electrical System Parts List > ................81 SPD-M20A Service Manual...
Page 6
Table of Contents 8.3 Optional List ................81 CHAPTER 9: PARTS TABLE ..........82 9.1 SPD-M20A (P/N 228-45005) ............. 82 9.2 Optical Unit (OPTICAL UNIT P/N 228-42510) ......85 9.3 Control Unit (CONTROL UNIT P/N 228-42511) ......87 9.4 Temperature Controlled Cell ASSY (P/N 228-42593) ....89 CHAPTER 10: CIRCUIT DIAGRAMS ........
<Scope of the Service Manual> This service manual describes maintenance procedures for the SPD-M20A photodiode array detector used in Shimadzu liquid chromatographs. The content of the service manual includes information concerning its operating principles and construction, a description of its control circuits and methods for testing its function and performance, and is compiled in a way that is easy to utilize by service personnel.
Page 8
P/N change of the maintenance part for "High Sensitivity UHPLC Optional Flow cell" 228-54357-45 -> 228-45609-41 Revision M: 7/Dec/2015 1 P/N of Cell window fixed screw ASSY and Heater SPD-20 ASSY is changed. Revision N: 26/Apr/2016 1 075-26489-11 IC,M95320-ZBN6 →S228-53010-41 PCB,EEPROM 32K SPD-M20A Service Manual...
CAUTION Used when there is the potential for physical damage to the instrument. NOTE Used when operational performance is improved, or understanding of explanatory content is facilitated. SPD-M20A Service Manual...
The operating temperature range of the entire system is influenced by the operating temperature range of the personal computer or printer. For the operating temperature ranges of the personal computer and printer, see the respective instruction manuals. SPD-M20A Service Manual...
(dark current) signal or when the filter is undergoing automatic wavelength correction or during an automatic wavelength check. The figure on the following page is a view of the optical system from above and from the side. SPD-M20A Service Manual...
Page 13
Mirror 2 (M2 ASSY) 20 25 mm Top view D2 lamp W Lamp (W Lamp ASSY) Half mirror (LM ASSY) 16 1T Mirror 1 (M1 ASSY) 20 25 3t Flow cell (Temperature controlled cell ASSY) Side view SPD-M20A Service Manual...
2) The internal parts are shown below. Optical system unit CPU board Main frame Duct Inside of main unit (with case cover removed) < Duct (P/N 228-42564)> SPD-M20A Service Manual...
3) Remove the connector that is attached to the leak sensor and panel from the CPU board. Positions of Leak Sensor and Panel Connector 4) Remove the two sets of two screws (M3x6) that fasten the hinges for the front panel and remove the hinges. Positions of Front Panel Fixing Screws SPD-M20A Service Manual...
NOTE When adjusting, use the following jigs. ・ Jig, right upper hinge (P/N 228-43222-91) ・ Jig, right lower hinge (P/N 228-43223) Front panel adjustment procedure diagram Attaching the control panel ASSY (right door) SPD-M20A Service Manual...
Page 20
Temporarily placing the right hinge (top and bottom) 3) Attach the right panel ASSY, and place the upper left part of the right door on of the diagram below. Tighten the screw lightly. Detail SPD-M20A Service Manual...
Page 21
[B][D], and hold while tightening the screws. Detail 3-(1)/(2) Detail Attaching the lower right hinge 7) Insert the lower right hinge jig (P/N 228-43223) in 7 of the diagram below (hatched parts in the diagram). Detail 7 SPD-M20A Service Manual...
Page 22
Due to the gutter between the hinge and right panel, the right panel will lean under its own weight and will contact 3 to align the top surface ( about 0.1 mm depending on the intersection). SPD-M20A Service Manual...
Page 23
Chapter 5: Assembly and Disassembly of Parts 9) When attached, check the following. SPD-M20A Service Manual...
Positions of Respective Sensor <Leak sensor ASSY 700 MM> 1) Remove the leak sensor connector connected to the CPU board. Leak sensor connector 2) Remove the screw (M3x6) fixing the front panel hinge, and remove the hinge. SPD-M20A Service Manual...
The screw fixing the sponge (plate) also tightens the guide fin for the W lamp housing underneath, and should not be removed. 2) Remove the thermistor connector connected to the CPU board. Thermistor connector 3) Remove the screw (sems, P3 M3x6) fixing the thermistor, and detach it. <Temperature controlled cell connector> SPD-M20A Service Manual...
Page 26
2) Remove the two screws (M3x6) fixing the temperature controlled cell connector, and remove the cell connector together with the holder 3) Remove the screw (M2.5x8) fixing the holder and the temperature controlled cell connector, and remove the temperature controlled cell connector. SPD-M20A Service Manual...
When replacing CPU board, it is necessary to insert it onto the new board. NOTE The EEPROM is not included on the replacement boards (board, PDA-CPU P/N 228-41823-91). Transfer the EEPROM on the old board to the new board. SPD-M20A Service Manual...
Page 28
3) Remove the spacer bolts to the left and right of the RS-232C connector. Positions of RS-232C Connector Fixing Bolts 4) Remove the four screws (sems, P3 M3x6) fixing the CPU board to the plate, and remove the CPU board. SPD-M20A Service Manual...
1) Remove the CPU board (see 5.1.5.1 CPU Board (PCB ASSY, PDA-CPU P/N 228-41820-91) ). 3) Remove each connector from the power supply board. 2) Remove four screws (sems, P3 M3x6) fixing the power supply board, and detach the power supply board. Positions of Power Supply Board Fixing Screws SPD-M20A Service Manual...
Bands for the ground and cable on the case cover. 3) Remove the three screws (truss head, M4x15) that attach the optical system unit, and slowly lift out the optical system unit. Positions of Optical System Unit Fixing Screws SPD-M20A Service Manual...
Page 31
33193) of the optical system unit, and remove the cover. Positions of Side Cover Fixing Screws 7) The inside of the cover is shown below. Half mirror LM ASSY P/N 228-33183-91 M1 ASSY P/N 228-33184-91 Optical system unit interior SPD-M20A Service Manual...
P/N 228-42561 Positions of PDA Holder Fixing Screws 5) Remove the PDA with the special tool (retraction tool, GX-3 P/N 086-16671-01, etc.). 6) To adjust the new PDA after installation, see 6.8 Photodiode Array (PDA) Adjustment. <Slit ASSY> SPD-M20A Service Manual...
The mirror with a mask attached to the mirror is the M1 ASSY. < Mirror M2 ASSY> 1) Remove the two screws (sems, P4 M4x8) fixing the M2 ASSY, and remove the M2 ASSY. Positions of M2 Fixing Screws 2) To adjust after installation, see 6.4 Mirror 2 Adjustment. SPD-M20A Service Manual...
Be careful to avoid contamination of the filter (P/N 228-42613). Filter P/N 228-42613 <W lamp ASSY> 1) Remove the screw (sems, P3 M4x30) fixing the W lamp housing, and remove the lamp housing. Positions of W Lamp Housing Fixing Screws SPD-M20A Service Manual...
1) Remove the single nut (M4) on the side of the case and the two nuts (M4) on the side of the lamp housing which attach the lamp housing. D2 lamp housing nut (left) and case nuts (right) <Half mirror (LM ASSY)> SPD-M20A Service Manual...
Simultaneously, detach the connectors that lead the cables around the lamp housing fan. 2) Remove the duct. 3) Remove the screws (sems, P3 M3x35) fixing the lamp housing fan, and detach Connector Positions of Lamp Housing Fan Fixing Screws <Fuses and Power Supply Voltage> SPD-M20A Service Manual...
Page 37
Fuse holders Voltage selector NOTE For details regarding fuse replacement and power supply voltage switching, see the Instruction Manual "8.7 Fuse Replacement, "9.1.3 Power Supply Connection". SPD-M20A Service Manual...
When performing adjustment of the optical axis, be sure to wear gloves. CAUTION Be careful to avoid scratching or contaminating mirrors, gratings, quartz plates, lenses, PDA and other optical elements. Tools Required for Adjustment and Testing SPD-M20A Service Manual...
5) Check the connection to the PC. NOTE For details regarding network settings, see the instruction manual "5.3.3 Network Settings". For details regarding wiring, see the instruction manual "9.1.6 Wiring". < Filling Flow Cell with Water > SPD-M20A Service Manual...
3) Pressing it once more lights the W lamp alone. 4) Pressing it once more turns off both lamps. 5) Thereafter, each time it is pressed, the sequence repeats from step 1) to 4) (D2→ D2+W → W → OFF). SPD-M20A Service Manual...
Former jig New jig The new-model mercury lamp holder consists of the following parts. Set screw Frosted glass (Frosted glass side is on the lamp side.) Slit Set screw Packing SPD-M20A Service Manual...
6.3.1 Process when Performing Wavelength Calibration The SPD-M20A has 1.2 nm/8 nm wide slits to adjust the optical path. When wavelength calibration is performed, the reference point of PDA element is determined using the 656 nm light using the 1.2 nm slit and span adjustment (wavelength assignment for each PDA element) is performed using the 360 nm light.
4) Adjust the light image so that is on the center point of the target cell mark using the rotation direction adjustment screw and elevation angle adjustment screws. 5) Tighten the M1 ASSY installation screws. 6) Loosen the rotation direction adjustment screw. 7) Tighten the nut on the rotation direction adjustment screw. SPD-M20A Service Manual...
・ Should be no distortion of image shape 6.3.4 W Lamp House Adjustment (W Lamp Adjustment, Please read Instruction manual) 1) Turn OFF the D2 lamp, and turn ON the W lamp. 2) Attach the mask M1-L. SPD-M20A Service Manual...
Y direction is 1 mm away from each other, move the mirror of the M2 1 mm closer to the slit ASSY. 4) Loosen the slit ASSY fixing screws and remove the slit ASSY. Do not pull out the connector. SPD-M20A Service Manual...
1) Press the slit ASSY in the Y direction shown in the figure below and move it in the X - X' direction so that the light spot on the slit aligns with the slit. SPD-M20A Service Manual...
Adjust the rotation direction so that the end of the 0 order light contacts the 0 order light mark. 0次光 0 order light 5) Fix the GR ASSY and mount the mask GR. 6) Replace the target PDA with the PDA ASSY. SPD-M20A Service Manual...
ASSY at the position where the half bandwidth of the emission line becomes 1.18 ele max. 15) Adjust the position of the emission line peak within a range 60 ele to 63 ele (maximum adjustment range: 52 ele to 72 ele). SPD-M20A Service Manual...
60 ele and 63 ele. Adjust the position of the 0 order light described in 6.3.8 Grating Adjustment so that the emission line is in such position. If the emission line is at 52 ele to 59 ele, move the 0 order light to the left. SPD-M20A Service Manual...
If the mask M1-L is off to the front, it may cause wavelength gap. Make sure that it is positioned at the correct position. *Type dose not have a groove in the base. *Type there is a groove in the base SPD-M20A Service Manual...
2) “regedit” is inputted into a name and [OK] is clicked. 3) The right click of the “HKEY_LOCAL_MACHINE\SOFTWARE\Shimadzu\Labsolutions” is chosen and carried out. 4) [Rename] is chosen and “Labsolutions” is changed into “Labsolutions_a” etc.. 5) Please return after use to the original name. SPD-M20A Service Manual...
Chapter 6: Maintenance and Adjustments < When PDA Utility software of SPD-M20A attachment is installed > 1) [Start], [Programs], [PDA Utility], [PDA Utility] are chosen and started. NOTE It is the same as CLASS-VP < Select connect instrument >...
When SPD- M10Avp is used, please be sure to choose the system which suited the software used. It is safer to choose, although it was not required in the case of SPD-M20A. < The service menu starting method> NOTE ...
< Optical Check > 1) The menu for PDA Utility service menu. 2) An “Optical Check” button is pushed. An “Optical Check” window is displayed. 3) The contents of the menu for service “Function” are as follows. SPD-M20A Service Manual...
Stray parameters is initialized. < Leak Sensor Check / Calibration > 1) The menu for PDA Utility service menu. 2) The “Leak sensor check / Calibration” button is pushed. The “Leak sensor check / Calibration” window is displayed. SPD-M20A Service Manual...
1) The menu for PDA Utility service menu. 2) The “Instrument Information” button is pushed. The “Instrument Information” window is displayed. Serial number and manufact date can be inputted on this screen. ROM Version, ExposureTime, and W Lamp Voltage are automatically changed after upgrade. SPD-M20A Service Manual...
In the "leak" state, and read the "Sensor Output" of the sensor..............B 7) Calculate the "Threshold" using the following expression, and set this threshold........................(R = 0.7 (B A) A SPD-M20A Service Manual...
0.5 AU and 2.0 AU solutions should be specialized for that purpose. Balance For weighing the caffeine. Disposable syringes (2 mL) Adaptors for disposable Male nuts (PEEK), couplings, etc. syringes < Absorbance Linearity Calibration (Stray Light Correction) Procedure > SPD-M20A Service Manual...
Page 65
Only D2 lamp Lamp: turned ON Cell Temperature: 40C 4) Select [Function] and perform [Stray Light Correction]. 5) Proceed through <Content> --> <Background Compensation>. 6) Use a syringe to inject 1 mL of methanol into the cell. SPD-M20A Service Manual...
Page 66
9) After confirming that the baseline is stable, press [Next]. 10) The spectra after the respective 1.2 nm and 8 nm slit width corrections are displayed. If there is no problem, press [Next] and set the correction value in the instrument. SPD-M20A Service Manual...
14) If the error does not satisfy the criterion, repeat the linearity correction procedure. ・ If the error (E) is +4% or more, narrow the light measurement wavelength bandwidth setting to 10 to 14 nm, and repeat the linearity correction procedure. SPD-M20A Service Manual...
20 L loop (about 50 L minimum to fill the loop), and measure the peak widening on a recorder. Perform the measurement twice, and confirm there is no difference in results. Time constant 640 ms Slit width 1.2 nm Wavelength 250 nm SPD-M20A Service Manual...
Page 69
When performing injection, be careful not to allow bubbles to enter. W is less than 10 seconds (with INERT cell, less than 15 Criteria seconds) b/a 2 W: Peak width b/a: Peak degree of warp SPD-M20A Service Manual...
Chapter 6: Maintenance and Adjustments 6.10 Version Upgrade There are 2 ways to upgrade the SPD-M20A, one via a LAN and the other via RS-232C connection. If the SPD-M20A is connected to a network, the upgrade can be performed via the LAN, and if this method presents problems or if there is no network, the upgrade can be performed via RS-232C connection.
Chapter 6: Maintenance and Adjustments NOTE For the SPD-M20A, the version upgrade file name is in the format "m20aXXX.rom" (where XXX indicates the version number). 7) During the version upgrade process, the status indicator on the front of the instrument continues blinking.
6) The instrument's LED enters the state described in step 2) of "6.10.2 Upgrade by Way of RS-232C (1)". Exit the terminal software, and complete the version upgrade by performing the steps from 3) to the end of the procedure in "6.10.2 Upgrade by Way of RS-232C (1)". SPD-M20A Service Manual...
0x8922 Error occurred during An error occurred during the Perform system check again. If same system check system check. Lamp failure, error occurs again, contact Shimadzu etc., occurred. Representative. 0x4001, Detector buffer memory LAN or PC breakdown Restart the instrument and the PC.
Measurement start time is Restart the instrument and the PC. abnormal, or there is a bug. 2100H Internal error An error occurred during the Perform system check again. If same system check. Lamp failure, error occurs again, contact Shimadzu SPD-M20A Service Manual...
Page 75
W lamp or circuit board is damaged, etc. power supply printed circuit board. C014H W lamp error By failure of Power board, Power board is exchanged. the voltage of W lamp is excessive. SPD-M20A Service Manual...
・ If there is a ping response even if the SPD-M20A is turned OFF, the IP address may be duplicated. ・ If there is a ping response from a PC on the same network as SPD-M20A, but no response from a PC on the different network, check the SPD-M20A subnet mask and default gateway settings.
Page 77
Chapter 7: Fault Diagnosis and Corrective Action ・ If they are not on the same network, communication is possible only via a router, etc. Change the IP address setting of the PC or the SPD-M20A. SPD-M20A Service Manual...
Chapter 8: System Configuration and Parts List 8.1. Consumable Parts List Interchangeab Part Name Remark ility with VP 228-34016 D2 lamp (deuterium Lamp) Common Ultraviolet light source 228-34410 W lamp (tungsten lamp) Common Visible light source Cell gasket 6 3 0.1 228-35097-03 Gasket for flow cell 228-14572...
< Temperature controlled semi-micro flow cell Parts List > Part Name Remark Temperature controlled semi-micro flow 228-45605-92/94/44 - 92:Old Cell cell 228-40239-91 Lens fixing screw ASSY Lens fixing screws for flow cell 228-14572 Lens Incident light side lens for flow cell 228-18058 Cell window Emission light side cell window for flow cell...
< High Sensitivity UHPLC Optional Flow cell > Part Name Remark For Fast Cell ID 1.0 Gasket 228-35097-01 For Fast Cell ID 0.8 Gasket 228-35097-05 Lens 228-14572 Cell window 228-18058 Lens Fixing Screw 228-40239-91 ASSY Inlet tubing ASSY 228-45609-41 Mail nut and Ferrule are attached. Mail nut,0.8MN-M4 228-42605 Ferrule,0.8F...
0.2 mm 228-34189-93/43 Fraction collection cell (Variable length cell) path length: 0.1 mm 228-25293-93 Semi-micro cell 228-45618-49 Fast Cell for SPD-M20A, ID 1.0 228-45618-46 Fast Cell for SPD-M20A, ID 0.8 Custom-made item 228-39306-91 Analog cable (RCA jack cable) 228-42622-91...
Chapter 9: Parts Table 9.1 SPD-M20A (P/N 228-45005) Top panel, Side Panel Rear Panel...
Page 85
Duct 228-30163-80 Certificate of compliance, SPDM20 228-33627 Certificate of compliance, entry directions 228-30164 Certificate of compliance, instructions (-01) Name plate, SPD-M20A 100V 228-42604 (-02) Name plate, SPD-M20A 120V (-03) Name plate, SPD-M20A 230V 228-90052 GLP test result certificate 072-60333-03 Clip, wire SD-15...
Page 86
037-02820-55 Knob, knurled N-1 M3 x 14 white 228-53010-41 PCB,EEPROM 32K 020-37057 Screw, SUS truss head M4X15 020-37054 Screw, SUS truss head M4X8 020-37501 Screw, SUS binding head M3X6 020-37503 Screw, SUS binding head M3X10 021-60314-01 Tapping screw, SUS pan head M3X10 023-66130 Washer, SUS flat M3 228-39613-03...
9.2 Optical Unit (OPTICAL UNIT P/N 228-42510) Optical Unit (Top Panel, Front Panel) Parts Table Part Name 228-33300-02 Base 228-42587-91 Cover ASSY 228-33193 Side plate 228-33183-91 LM ASSY 228-33184-91 M1 ASSY 228-33140-91 Shutter ASSY 228-33189-92 W lamp ASSY, M20A...
9.3 Control Unit (CONTROL UNIT P/N 228-42511) Control Unit (Top panel, Side Panel, Rear Panel)
Page 90
Control Unit (Inside Top Panel) Parts Table Part Name 228-42511 CONTROL UNIT 228-42513 Chassis 228-25436 Rubber feet 228-42582 Name plate, rear 228-42562 018-17101-04 Shield, finger 97-538-02 228-42515 Duct 228-42527 Holder, bearing SW 037-38760-04 Bushing, D4250-9130 228-42565 Shaft, SW 026-66203 Snap ring, for shaft E3 228-42543 Holder, SW 075-00070-87...
Need help?
Do you have a question about the SPD-M20A and is the answer not in the manual?
Questions and answers