Shimadzu SPD-40 CL Instruction Manual
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Shimadzu SPD-40 CL Instruction Manual

Shimadzu SPD-40 CL Instruction Manual

Uv-vis detector for shimadzu liquid chromatograph
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228-97200A
Jun. 2022
UV-VIS Detector
For Shimadzu Liquid Chromatograph
SPD-40 CL
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

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Summary of Contents for Shimadzu SPD-40 CL

  • Page 1 228-97200A Jun. 2022 UV-VIS Detector For Shimadzu Liquid Chromatograph SPD-40 CL Instruction Manual Read this manual thoroughly before you use the product. Keep this manual for future reference.
  • Page 2 This page is intentionally left blank.
  • Page 3 Shimadzu representative to request a replacement. • To ensure safe operation, read the accompanying booklet "Safety Guideline" before using the product. • To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, re-installation (after the product is moved), or repair is required.

  • Page 4: Introduction

    • All rights are reserved, including those to reproduce this manual or parts thereof in any form without permission in writing from Shimadzu Corporation. • Nexera is a trademark of Shimadzu Corporation. Windows is a registered trademark of Microsoft Corporation in the United States and/or other countries.

  • Page 5: Electromagnetic Compatibility

    Electromagnetic Compatibility Descriptions in this section apply only to the following models: • 228-65312-55 SPD-40 CL This product complies with European standard EN61326, class B for electromagnetic interference (Emissions) and industrial electromagnetic environment (Immunity).

  • Page 6: Table Of Contents

    List of Auxiliary Functions (FUNCTION) ....... 3.6.2 Showing the Auxiliary Function Screen ........3.6.3 [PARAMETER] Setting Group ........3.6.4 [CONTROL] Setting Group ........3.6.5 [SYSTEM] Setting Group ........3.6.6 [UTILITY] Setting Group .......... 3.6.7 [EDIT] Setting Group VP Functions ............. SPD-40 CL...
  • Page 7 Removing/Installing the Flow Cell ............. 4.2.1 Removing the Flow Cell ........4.2.2 Installing the Flow Cell Flow Cell Inspection/Cleaning ......Disassembling and Simple Cleaning the Flow Cell ..............Replacing a Flow Cell Part ......... Storing the Flow Cell ......... SPD-40 CL...
  • Page 8 4.11.1 Usual cleaning ........4.11.2 Decontamination cleaning Technical Specifications ............ Information ........5.1.1 Performance Specifications ..........5.1.2 Standard Flow Cell ..........5.1.3 Detector Unit Consumable Parts/Maintenance Parts/Replacement Parts .................. 5.2.1 Consumable Parts ..........5.2.2 Maintenance Parts Index ............SPD-40 CL...

  • Page 9: Overview

    Overview The Shimadzu SPD-40 CL is an ultra-violet visible spectrophotometric detector, for use in liquid chromatography. This instrument uses a deuterium lamp as its light source and is intended primarily for analyses in the ultraviolet region. In this instrument, the sample is irradiated with light...

  • Page 10: Component Parts

    1 It contains cautions for use regarding the instrument. Optional Parts Optional parts available for this instrument are listed below. For information about other optional parts listed below, contact your Shimadzu representative. For features of each optional flow cell part, refer to "1.3.1 Optional Flow Cell"...

  • Page 11: Optional Flow Cell

    228-64725-42 A cell for semi-micro analysis. 1.3.1 Optional Flow Cell For details on the specifications, refer to the instruction manual of each optional flow cell. n Flow cell (Standard flow cell for SPD-40 CL) 228-62156-42 Item Features Optical path length...
  • Page 12 4.3 µL：I.D. 0.1 mm, length 550 mm (The length out of the cell Inlet tube is 315～340 mm.) Outlet tube 2.1 µL：I.D. 0.13 mm, length 160 mm Wetting part materials SUS316L, quartz, PFA, PEEK Cell pressure tolerance 12 MPa Cell temperature 〇 adjustment SPD-40 CL...

  • Page 13: Parts Identification And Function

    This cover must be closed during analysis. Switches ON/OFF the power. Pressing the switch turns on the power and pressing (Power button) it again turns off the power. The switch is enabled only when the main power switch on the back is on. SPD-40 CL...

  • Page 14: Behind Front Cover, Top, And Left Side

    Connect this outlet tubing to the drain tubing to flow Cell outlet tubing mobile phase used for analysis. Cell inlet tubing Connect to the column. (marked with red The length out of the cell is 315～340 mm. cover) SPD-40 CL...
  • Page 15 2.2 Behind Front Cover, Top, and Left Side Name Description Coupling holder Fixes the coupling of the cell outlet tubing. SPD-40 CL...

  • Page 16: Back

    If the system controller is connected and the main power switch is on, the user can use (power button) of the system controller to turn on/off the power from the front of the instrument. If the instrument is not used for a long time, turn off the main power switch. SPD-40 CL...

  • Page 17: Right Side And Bottom

    Name Description The instrument leaks drain from this port to the system component below. Leakage drain outlet System Guide "7.1 Installation" Fixes the inside of the instrument to prevent the shock Shipping screw during transportation. Remove before installation. SPD-40 CL...

  • Page 18: Names And Functions Of Display And Operation Area

    • Analysis ready: Illuminates in green • Error: Illuminates in red • Analysis in progress: Illuminates in blue Status LED • Preparation for analysis in progress: Illuminates in yellow • Sleep mode: Illuminates in orange • During update: Blinks in blue SPD-40 CL...
  • Page 19 *1 If CBM-40 CL is used, pressing the power button of the pump turns off the power of the entire system. Note, however, in the case of a system with multiple pumps, the system power can be turned off using the power button of the pump with the smallest optical link number. SPD-40 CL...

  • Page 20: Display Area

    Display [D2] , indicating the deuterium (D2) lamp is on. status Illuminates in the reverse polarity output mode (when the polarity is set to "-"). Polarity "Polarity" P.18 LAMP SCAN/MARK Not used in CL model. Highlighted during execution of a time program. ZERO SPD-40 CL...

  • Page 21: Operation Area

    Moves the cursor or switches the screen [↓][←] [·] to [9] Numeric Keys Inputs values for the setting items. Minus Key Used for inputting the parameters. *1: These keys cannot be operated while initializing the instrument at startup. SPD-40 CL...

  • Page 22: Operation

    Buffer solutions crystallize upon evaporation, and can clog the flow cell and tubing. Turning Power ON/OFF n Turning the Power ON Press and hold (power button) for at least 3 seconds. Power is supplied. SPD-40 CL...
  • Page 23 If an alarm sounds and a [WAVE CHECK NO GOOD] message is displayed on the screen: No peak has been detected within 1nm of 656nm or 254nm. To stop the alarm, press [CE]. Verify that the cell is installed properly. "4.2 Removing/Installing the Flow Cell" P.63 SPD-40 CL...
  • Page 24 When the calibration is complete, the [WAVE CHECK] will be run automatically to recheck the wavelength accuracy. If [WAVE CHECK NO GOOD] appears once again, turn off the power of the instrument and contact the nearest Shimadzu branch/sales office or agent.

  • Page 25: Setting Parameters

    0.1 ℃ 0.2 ℃ ready check range Do not change the factory default value. Parameters are set in the [PARAMETER] setting group of the auxiliary functions screen (FUNCTION). "3.6 Auxiliary Functions Screen (FUNCTION)" P.25 "3.6.3 [PARAMETER] Setting Group" P.27 SPD-40 CL...
  • Page 26 (using the indirect absorbance detection method), set the polarity to "-". "Setting the [POLARITY]" P.29 is displayed in the lower right portion of the MONITOR initial screen when the polarity is "-". SPD-40 CL...
  • Page 27 The ready check ensures that analysis starts after heat balance is established at temperature-controlled locations. The ready check is available for the flow cell and polychromator. "Setting the range where the cell temperature control is considered ready [CELL READY]" P.33 SPD-40 CL...

  • Page 28: Types Of Screens

    [FUNCTION] or VP functions screen [VP]. Move the cursor to the desired option with [↓] or [↑] and press [→] to display the screen. Press [←] to return to the previous screen. Press [CE] to return to the initial screen. SPD-40 CL...

  • Page 29: Monitoring Screens

    *1 Sample side: Refers to the light passing through the mobile phase and specimen.*1 Sample side: Refers to the light passing through the mobile phase and specimen. Reference side: Refers to the light used as the reference light without passing through the mobile phase. SPD-40 CL...
  • Page 30 The first row displays the output level of the sample light beam of the wavelength to be measured 1. The second row displays the output level of the reference light beam of the wavelength to be measured 1. SPD-40 CL...
  • Page 31 "[D2 LAMP USED TIME]" P.39. The D2 lamp used time is automatically reset when the D2 lamp is replaced. n Screen to display the total operation time [TOTAL OP TIME] Displays the total operation time of the instrument. SPD-40 CL...
  • Page 32 Screen to display the time that elapsed after a time program was started [PROG TIME] Displays the time that elapsed after a time program was started. The screen displays a maximum of 999.99 h. "3.10 Creating Time Program" P.54 SPD-40 CL...

  • Page 33: Auxiliary Functions Screen (Function)

    Sets the output polarity. P.29 AUX RANGE Numeric keypad Not used in CL model. RANGE Numeric keypad Not used in CL model. REC MODE Numeric keypad Not used in CL model. BL OFFSET ITG Numeric keypad Not used in CL model. SPD-40 CL...
  • Page 34 Sets the cell temperature control CELL READY Numeric keypad P.33 ready check function. DIRECT KEY3 SET Numeric keypad Not used in CL model. n [UTILITY] setting group Command Operation Function Page KEY CLOSE [Enter] Locks key input. P.34 SPD-40 CL...

  • Page 35: Showing The Auxiliary Function Screen

    The first item in the selected group appears. (Example: In the [PARAMETER] setting group) Press [↑] or [↓] to select a setting screen. Press [CE] to return to the initial screen. 3.6.3 [PARAMETER] Setting Group This group is for setting detector parameters. SPD-40 CL...
  • Page 36 [1] to switch the mode to single-wavelength chromatogram mode or press [2] to switch the mode to dual-wavelength mode before pressing [Enter]. The value displayed in the upper right is "1" in single-wavelength mode while the value is "2" in dual-wavelength mode. SPD-40 CL...
  • Page 37 This function is not used in the CL model. n Setting the [POLARITY] Setting “-” to polarity inverts the polarity of absorbance of the recorder output terminal and integrator output terminal from positive (negative) to negative (positive). Usually set as “+”. SPD-40 CL...

  • Page 38: Control] Setting Group

    After the result is displayed, the wavelength accuracy is checked. When the wavelength is confirmed to be accurate, [WAVE CHECK GOOD] is displayed. The figure shows that the wavelength accuracy is 0.3 for the absorbance peak of the SPD-40 CL...
  • Page 39 System Guide "5.4 Error Message List" How to measure the wavelength accuracy SPD-40 CL use zero-order light (0 nm) and the emission line (656.1 nm) of a deuterium lamp (D2 lamp) for calibrating the wavelength. The wavelength accuracy can be secured by using those wavelengths as the reference for adjustments.

  • Page 40: System] Setting Group

    Operation of the power button from the instrument is disabled according to the control of the system controller only. (Initial value) In addition to being controlled by the system controller, it is also possible to turn off the power individually from the instrument. SPD-40 CL...
  • Page 41 This function allows setting the range where the cell temperature control is considered ready. The screen displays the current setting in the upper right. Press [Enter] to display the numeric keypad, then input the values and press [Enter]. SPD-40 CL...

  • Page 42: Utility] Setting Group

    The key input sound, as well as the alarm sound to go off when an error occurs, are activated. (Default setting) Only the alarm sound that goes off when an error occurs is activated. All the buzzer sounds are deactivated. SPD-40 CL...

  • Page 43: Edit] Setting Group

    When 0 minutes is input, the screen is always lit, which could lower the life of the display screen. When the screen approaches its lifetime, the screen is gradually darkened, it does not suddenly disappear. 3.6.7 [EDIT] Setting Group Use this group to create a time program. "3.10 Creating Time Program" P.54 SPD-40 CL...

  • Page 44: Vp Functions

    Display Displays error log P.40 n Validation [VALIDAION] Support Group Command Operation Function Page Display・Numeric DATE Displays/sets the date P.41 keypad Display・Numeric TIME Displays/sets the time P.41 keypad LEAK SENSOR CHECK Display Runs check on leak sensor P.41 SPD-40 CL...

  • Page 45: Displaying The Vp Functions

    To specify whether to check the INITIAL 241nmCHK Numeric keypad wavelength accuracy at 241 nm during P.48 start-up of the instrument. Z WAVE Numeric keypad Not used in CL model. 3.7.2 Displaying the VP Functions Press [CE]. The initial screen is displayed. SPD-40 CL...

  • Page 46: Product Information Group

    The default password is "00000". 3.7.3 Product Information Group This group provides information about the instrument. n [SERIAL NUMBER] Shows the serial number of this instrument. n [Version] Shows the name of software (same as the model name) and version. SPD-40 CL...

  • Page 47: Maintenance Information Group

    12 hours) As for D2 lamp, it is impossible to reset the used time because the lamp itself memorizes the used time. The replacement alert time of the D2 lamp is 2,000 hours. "4.7 Replacing the Lamp" P.76 SPD-40 CL...
  • Page 48 Log10 in sequence, and return to the title screen. The figure shows an example that liquid leakage was detected on May 12, 2003. If less than 10 logs are recorded, the screen displays [No more Logs]. Press [CE] to return to the title screen. SPD-40 CL...

  • Page 49: Validation Support Group

    Example: Inputting 12:09:35 a.m. Press [Enter] to display the numeric keypad. Enter hour, minute and second in 2 digits and press [Enter]. The display uses the 24-hour clock. n [LEAK SENSOR CHECK] Carries on the operation test for leak sensor. SPD-40 CL...
  • Page 50 • Lamp operating time [D2 TIME] When all of checks pass, [CHECK GOOD] is displayed. 1: When the check fail, [CHECK NO GOOD] is displayed. 2: When the check fail, [CHECK WARNING] is displayed. Press [CE] to erase the resulting display. SPD-40 CL...
  • Page 51 The displays on the right appear alternately. Then, measure the mobile phase. After enclosing the mobile phase, press the [Enter] key. The display on the right appears for about 30 seconds. The wavelength of the mobile phase is measured. SPD-40 CL...

  • Page 52: Calibration Support Group

    Password should be registered by a system manager. Be sure to input five numbers. The default password is [00000]. When the password matches, the [OPERATION MODE] screen below appears on the screen. If the password is not input correctly, succeeding steps cannot be accessed. SPD-40 CL...
  • Page 53 [On] from [Off]. When [On] is set, the password screen is displayed. When this setting is changed or inputting the password releases the lock, the date is recorded in operation log. "[OPERATION LOG]" P.40 SPD-40 CL...
  • Page 54 The password must consist of five digits. To confirm, input the same password again. When the new password is registered, [PASSWORD CHANGED] is displayed. If not, [PASSWORD WRONG] is displayed. In this case, the password newly entered is not registered. SPD-40 CL...
  • Page 55 Recalibration operation is executed by service personnel. Press [Enter]. The instrument's wavelengths will be calibrated automatically. When calibration is complete, the [WAVE CHECK] (wavelength accuracy check) will be run automatically, and the result ([GOOD] or [NO GOOD]) displayed. SPD-40 CL...
  • Page 56 Specifying whether to check the wavelength accuracy at 241 nm during start-up [INITIAL 241nmCHK] This function allows specifying whether to check the wavelength accuracy at 241 nm during start-up. If a mobile phase with SPD-40 CL...
  • Page 57 Press the [Enter] key to display the numeric keypad. Enter the number you want to set, and press the [Enter] key again. After the wavelength accuracy test, be sure to return to the original setting "0". SPD-40 CL...

  • Page 58: Showing/Setting Cbm Parameter

    Shows the serial No. of CBM which controls the instrument. On the [CBM PARAMETER] screen, press [↑] or [↓] repeatedly until [SERIAL NUMBER] is displayed. The serial number of the system controller is shown on the bottom line. SPD-40 CL...
  • Page 59 Press [Enter] to display the numeric keypad, then input the values and press [Enter]. Set Value Transmitting speed Auto Detect 10 Mbps, Half Duplex 10 Mbps, Full Duplex 100 Mbps, Half Duplex SPD-40 CL...
  • Page 60 Setting the subnet mask [SUBNET MASK] Sets subnet mask of CBM which controls the instrument. On the [CBM PARAMETER] screen, press [↑] or [↓] repeatedly until [SUBNET MASK] is displayed. The current set value is shown on the bottom line. SPD-40 CL...

  • Page 61: Measuring In Single Wavelength Mode

    Please set the value to "0" in the CL model. Measuring in Single Wavelength Mode In the CL model, the parameters of this function are set by LabSolutions. Operation in Dual Wavelength Mode In the CL model, the parameters of this function are set by LabSolutions. SPD-40 CL...

  • Page 62: Creating Time Program

    Sets polarity of detector output 1: Reversed polarity 0: OFF LAMP Turns D2 lamp ON/OFF 1: D2 lamp ON 0: OFF CELT Sets temperature of the flow cell Room temperature 9 °C - 50 °C S.V. Not used in CL model SPD-40 CL...

  • Page 63: Setting Time Programming Parameters

    3.10 Creating Time Program Setting ranges for l1 and l2 Wavelength Mode Model Setting Range Single-wavelength SPD-40 CL 190 to 700 nm measurement mode Dual-wavelength 190 to 370 nm or SPD-40 CL measurement mode 371 to 700 nm 3.10.2 Setting Time Programming Parameters Sets parameters in the [EDIT] screen to create a time program.

  • Page 64: Creating A Typical Time Program

    The initial screen is displayed. Press [→] twice. Then, press [↑] or [↓] repeatedly until [EDIT] is displayed. Press [→] once. The time program edit mode screen is displayed. Press [Enter]. The time (minute) setting screen is displayed. Press [5] and [Enter]. SPD-40 CL...

  • Page 65: Repeating The Time Program Steps [Loop]

    • When setting several steps, they are ordered automatically in sequence. • Always end the program with a [STOP] command, unless the program is to run indefinitely and be stopped manually. 3.10.4 Repeating the Time Program Steps [LOOP] [LOOP] function can register the loop count of time program. SPD-40 CL...

  • Page 66: Deleting Steps

    There are 2 ways to stop the time program: • Press [run] to force a program in progress to stop. • Use the [STOP] command to force a program in progress to stop. "3.10.3 Creating a Typical Time Program" P.56 SPD-40 CL...

  • Page 67: Connection With The System Controller

    In the CL model, do not connect another external device. Wiring is done by service personnel during installation. SPD-40 CL...

  • Page 68: Periodic Inspection And Maintenance

    Periodic Inspection and Maintenance It is necessary to perform periodic inspections of this instrument to ensure its safe use. It is possible to have these periodic inspections performed by Shimadzu service representatives on a contractual basis. For information regarding the maintenance inspection contract, contact your Shimadzu representative.

  • Page 69: List Of Periodic Inspection And Maintenance

    Replace the lenses when some dirt Flow cell lens remains after disassembling and replacement cleaning the flow cell. Inspection of Every six to twelve months Wavelength Accuracy Inspection of Leak Every six to twelve months Sensor SPD-40 CL...

  • Page 70: Post-Inspection/Maintenance Leakage Check

    (The slot in cell window fastening screws is 1 mm wide and 8 mm long. The regular screwdriver part number is 086-10412-01.)　If tightening the cell window fastening screws does not resolve the leak, contact a Shimadzu representative. SPD-40 CL...

  • Page 71: Removing/Installing The Flow Cell

    When replacing with another cell, wavelength calibration is required after replacement. Wavelength calibration is performed by the service personnel, so contact your Shimadzu representative. 4.2.1 Removing the Flow Cell Open the front cover. Remove the coupling from the coupling holder (B).

  • Page 72: Installing The Flow Cell

    Connect the inlet tubing of the flow cell directly to the column. Via the coupling, connect the outlet tubing of the flow cell to the drain tubing. When performing the above operation, do not let any air enter the flow lines. Close the front cover. SPD-40 CL...

  • Page 73: Flow Cell Inspection/Cleaning

    Standard accessory 228-40998-10 Male nut 0.8MN-PEEK Standard accessory 228-46363 ― ― 2-propanol Remove the flow cell with connecting inlet / outlet piping. "4.2.1 Removing the Flow Cell" P.63 Set the flow rate to 1 mL/min and then start pumping. SPD-40 CL...
  • Page 74 Male nut 0.8MN-PEEK Cell inlet tubing Syringe needle Cell outlet tubing Syringe Male nut ETFE Plunger Coupling 1.6-0.8C Insert the syringe needle (G) on the tip of the syringe (H) and turn the syringe (H) clockwise to fix. SPD-40 CL...
  • Page 75 If an organic solvent is mixed with salts, crystals will form and clog the tubing. Flush the flow cell with distilled water or deionized water using a syringe to purge the rinsing solution (2-propanol). Perform step 3 to check for bubbles and dirt. SPD-40 CL...

  • Page 76: Disassembling And Simple Cleaning The Flow Cell

    Be sure to replace when Consumable 228-35097-95 (2 Gasket disassembling and cleaning the part pcs) flow cell. 2-Propanol (Mobile Phase Solution Used for Analysis) If use of the gaskets is continued without replacing, fluid may begin to leak. SPD-40 CL...
  • Page 77 Loosen the male nut (A) on the column side, and remove the flow cell inlet tubing (B). Remove the flow cell outlet tubing (C) in the same way. Remove the left and right setscrews (D) using a flathead screwdriver. SPD-40 CL...
  • Page 78 Put the removed lenses in 2-propanol and clean them with an ultrasonic bath for 5 minutes. Attach the cleaned lenses and two new cell gaskets to the cell. Use a piece of wiping paper soaked in 2-propanol to remove dirt inside the cell housing (G). SPD-40 CL...
  • Page 79 If there is a fluid leak, rinse the lenses again, and then tighten the setscrews again so that they are not loose. Loosen the male nut (A), then remove the coupling 1.6-0.8C (H) and the syringe needle. Attach the flow cell. "4.2.2 Installing the Flow Cell" P.64 SPD-40 CL...

  • Page 80: Replacing A Flow Cell Part

    Maintenance 228-73281-41 (2 pcs) Lens part 228-14572 (1 pc) 2-propanol If use of the gaskets is continued without replacing, fluid may begin to leak. Remove the flow cell. "4.2.1 Removing the Flow Cell" P.63 SPD-40 CL...
  • Page 81 Loosen the male nut (A) on the column side, and remove the flow cell inlet tubing (B). Remove the flow cell outlet tubing (C) in the same way. Remove the left and right setscrews (D) using a flathead screwdriver. Remove the lens (E) and gasket (F) using plastic tweezers. SPD-40 CL...
  • Page 82 If an air blower (for the lens) is not available, use a piece of wiping paper soaked in 2-propanol to remove the dust. Attach the new gaskets and lenses in that order using plastic tweezers, and attach the setscrews using a flathead screwdriver. SPD-40 CL...

  • Page 83: Storing The Flow Cell

    Before storing the flow cell, pump methanol into it. Keep the cell filled with methanol or aspirate the methanol in the flow cell with a syringe to dry it. Then, seal and inlet and outlet tubing of the cell with stop plugs. SPD-40 CL...

  • Page 84: Replacing The Lamp

    4 Maintenance Replacing the Lamp The SPD-40 CL has a D2 lamp. The lamps need to be replaced periodically. As the D2 lamp in this detector approach the end of their life, their intensity decreases and baseline noise increases. Replace the lamps with new ones using the guaranteed service life as a guide.

  • Page 85: Removal Of Top Cover And Radiating Fin Assembly

    Loosen the four screws (D) holding the radiating fin assembly in the lamp compartment, and remove the fin assembly (C). The D2 lamp (F) and mirror (G) will now be visible inside the lamp housing (E). The fin assembly's four screws cannot be removed. SPD-40 CL...

  • Page 86: Deuterium Lamp Replacement

    4 Maintenance Lamp installation layout 4.7.2 Deuterium Lamp Replacement Remove the connector (A) of the D2 lamp and then the lead wire (C) from the clamp (B). SPD-40 CL...
  • Page 87 Be careful not to break the lamp. Do not impart shocks or vibrations to the lamp. During replacement work, be sure to keep the surfaces of the mirror clean. A dirty surface of the mirror prevents accurate detection. SPD-40 CL...
  • Page 88 Be careful not to touch the glass parts directly with your hands when replacing the lamp. Wrap the lamp in cotton gauze when carrying it. Accurate readings cannot be taken when the glass surface is dirty. When the lamp is dirty, wipe it off with lens paper soaked in ethanol. SPD-40 CL...
  • Page 89 Failure to do so may cause the lead wire to be broken and result in malfunction. Attach the fin assembly and top cover of the lamp housing and detector respectively, and tighten the screws. Securely tighten the screw of the radiation fin. A loose screw may worsen the performance. SPD-40 CL...

  • Page 90: Wavelength Accuracy Calibration

    Wavelength Accuracy Calibration Measure the peak wavelengths in the emission line spectra produced by the deuterium lamp, and calibrate them to minimize wavelength variance. Do not use this function in the CL model. Recalibration operation is executed by service personnel. SPD-40 CL...

  • Page 91: Absorbance Accuracy Calibration

    For persistent stains, clean the exterior using the following procedure. Dip a piece of cloth in a dilute neutral detergent and twist firmly to remove excess liquid. Use this cloth to scrub the soiled area of the exterior surface of the instrument. SPD-40 CL...

  • Page 92: Decontamination Cleaning

    After handling the hazardous substances, use the following procedure. Clean with a cloth soaked in Aqueous ethanol solution (80%) or aqueous sodium hypochlorite solution (0.05%) and wrung tightly. Do not leave metal surfaces wet. This can cause rusting and discoloration. SPD-40 CL...

  • Page 93: Technical Information

    Technical Information Specifications 5.1.1 Performance Specifications Item SPD-40 CL Light source D2 lamp 190 to 700 nm Cut-off filter to eliminate second-order Wavelength range diffraction is automatically activated for the 371 to 700 nm range. Spectral bandwidth 8 nm Wavelength accuracy ±1 nm...

  • Page 94: Standard Flow Cell

    5 Technical Information 5.1.2 Standard Flow Cell Item SPD-40 CL Cell length 10 mm Cell volume 12 µL Cell pressure resistance 12 MPa Cell connection materials SUS316L, PFA (fluorine resin), Quartz, PEEK 1 to 13 (Using some mobile phases with pH 10 or...

  • Page 95: Consumable Parts/Maintenance Parts/Replacement Parts

    Breaking Voltage Part No. Consump- Frequency (indicated on Capacity tion SPD-40 CL the instrument) 100-240 V AC S228-65312-55 150 VA 50/60 HZ 50 A (100-240 V) *1 Mains supply voltage fluctuations are not to exceed 10 % of the nominal supply voltage.
  • Page 96 23, 39 Direct Key DISP OFF TIME UTILITY EDIT ERROR LOG Version INITIAL 241nmCHK WAVE CHECK Initial Screen WAVE MODE INITIALIZE PARAM Wavelength KEY CLOSE LAMBDA 1 LAMBDA 2 LAMP LEAK SENSOR CHECK LINK ADDRESS LOCAL MAINTENANCE LOG SPD-40 CL...

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