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Summary of Contents for Shimadzu SPD-16
- Page 1 QMM-0162 Aug 2013 UV-VIS DETECTOR FOR SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH SPD-16 Service Manual This service manual is for professional service engineers. Do NOT use this manual unless you have received proper training.
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- Page 3 Inspection and Maintenance ......... In an Emergency ..........During a Power Outage ........Disposal Precautions ..........Static Electricity Precautions ............2.9.1 Preventing Static Electricity Accidents Precautions for Mobile Phase Selection and 2.10 Use ..............Specifications Performance Specifications ......... SPD-16...
- Page 4 5.7.2 List of primary internal parts for optical unit 5.8 Flow cell assembly drawing and component parts list ....5.8.1 Assembly drawing for Standard flow cell ... 5.8.2 Component parts list for standard flow cell assembly SPD-16...
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Page 5: Table Of Contents
Performance Inspection ................6.2.1 product information group ....... 6.2.2 Maintenance information group ......... 6.2.3 Validation Support group ........6.2.4 Calibration Support group List of factory functions .......... Adjustment SPD-16... - Page 6 Contents Maintenance and adjustments Cleaning the temperature-controlled flow cell ..... .. 7.1.1 Simplified cleaning of temperature-controlled cell ......7.1.2 Disassembled cleaning of flow cell ......... 7.1.3 Assembling the flow cell ..
- Page 7 Electrical parts list ..........Optional Parts......................9.4.1 Inert cell ..........9.4.2 Preparative cell ... 9.4.3 Parts list and diagram for solvent recycle valve control . SPD-16...
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- Page 9 Introduction Scope of Service Manual This service manual is intended for service engineers certified by Shimadzu and describes maintenance procedures for SPD-16 UV-VIS detector used in Shimadzu liquid chromatographs. Actual maintenance work should only be performed by a service engineer that has received training for that instrument.
- Page 10 Indicates information provided to improve product performance. Indicates the location of related reference information. Product Applications Do NOT use the instrument for any other application other than high performance liquid chromatography. Using this instrument for any other purpose could cause accidents. SPD-16...
- Page 11 Not doing so could cause poisoning or fire. Install the instrument near a sink or other source of tap water. If solvent gets into the eyes or onto the skin, flush it away immediately. SPD-16...
- Page 12 • Air currents from heating or air conditioning equipment are not directed onto the instrument. • The instrument is not exposed to direct sunlight. • There is no vibration. • Humidity is maintained within 20 to 85 %. • There is no condensation. SPD-16...
- Page 13 Otherwise, fire, electric shock or malfunction could occur. If the cord is damaged, immediately contact your Shimadzu representative. Take measures to prevent the instrument from falling in the event of an earthquake or other disaster. Strong vibrations could cause the instrument to fall over, resulting in injury.
- Page 14 Always wear protective gloves when handling any toxic or biologically infectious samples. Do NOT bend any tubing of the instrument at the same position repeatedly. Doing so could cause the tubing to rupture or crack, which could result in solvent spraying out. SPD-16...
- Page 15 Take the following measures in the event of a power outage. Turn the power to the instrument OFF. When power supply resumes, check in accordance with 1.1.1 Preparation Connectors at the Rear of the Instrument (left side), and use the standard startup procedure to start the instrument. SPD-16...
- Page 16 Disposal Precautions When disposing of the instrument, contact your Shimadzu representative. If you dispose them yourself, do so in accordance with the processing standards determined by law, separately from general industrial waste and household garbage. Static Electricity Precautions Static Electricity Precautions Liquid chromatograph (LC) uses flammable organic solvent(s) as the mobile phase.
- Page 17 Ignition of flammable If this happens and an electrical conductor is brought substances within a certain distance of the container, an electrical discharge will occur, releasing thermal energy which will ignite any flammable gas of sufficient density in the vicinity. SPD-16...
- Page 18 This will ensure that the electrical charges of the container and liquid pass to the ground. • Accessories for this measure Grounding wire with Part No. S228-21353-91 clip 18-L metal container Part No. S038-00044 4-L metal container Part No. S038-00043-01 SPD-16...
- Page 19 If the liquid to be drained into the waste container is virtually non-conductive S/m or less), it will be necessary to add properly conductive, and therefore safe, liquid to the tank. This conductive liquid may be added beforehand. SPD-16...
- Page 20 If it is not possible to use a conductive waste liquid container, take the following precautions: Ensure that the end of the inflow tubing is always submerged inside the container. Also, place some type of grounded metal object, such as a ground wire connected to the instrument, into the liquid. SPD-16...
- Page 21 • Fluorine organic solvents such as • Acetone hexafluoroisopropanol (HFIP) • Tetrahydrofuran (THF) *1 There is no problem with temporarily using low-concentration aqueous solutions with an acetone concentration of 0.5 % or less, e.g. for the purpose of checking the performance of the gradient. SPD-16...
- Page 22 1 Reproducibility when the wavelength is changed after turning the power ON in single wavelength mode. ∗ 2 In single wavelength mode. ∗ 3 Noise level in dual wavelength mode varies depending on the wavelength. It may be necessary to use less sensitive detector settings (higher Range values) when performing dual wavelength analysis. SPD-16...
- Page 23 Item SPD-16 Zero adjustment Auto zero function, baseline shift function Polarity switching Possible Performs wavelength scanning with flow stopped. Data stored in three Wavelength scanning function files, one of which stores background. Background is subtracted from sample scans. File data is not saved up (lost when power is turned OFF).
- Page 24 The filter is inserted in front of the slit at the 371－700 nm range for model SPD-16. After the light beam passes through the slit, it is reflected and focused by mirror M2 and hits a holographic grating of 1600 grooves per millimeter, where it is separated into its spectrum. The light separated by the grating emerges as parallel light beams that are reflected by mirror M3 and are wide enough to straddle and enter both of the sample and reference cells.
- Page 25 A flow-through type structure. The reference cell is simply a hole that lets light pass through. Then wavelength is checked using the bright line of D2 lamp. The checked value and the value stored in the memory are compared and CHECK GOOD is displayed in the screen if they are equal. SPD-16 Light path schematic SPD-16...
- Page 26 DA converters (M30 and M31) and a portion of that voltage is sent via resistors to the AUX terminal as integrator output. Similarly, resistors are also used to output 10 mV signals as data recorder output. (Measurementsi d e) Schematic of signal processing circuits SPD-16...
- Page 27 “D2” will appear in the lower left part of the initial display screen Hi g hvol t age tri g gerci r cui t D2l a mp Fixed current D2 circuit Fixed voltage D2 heater circuit Lamp illumination circuit schematic SPD-16...
- Page 28 Inside the front cover, and its top and left sides Refer to the Instruction Manual for details. Right and bottom sides Refer to the Instruction Manual for details. Back side Refer to the Instruction Manual for details. Inside the instrument Refer to the Instruction Manual for details. SPD-16...
- Page 29 Inside the optical unit 5.7.1 Illustration of optical unit interior ○ ○ ○ ○ ⑨ ○ ○ ○ ○ ○ ○ ② Optical unit interior SPD-16...
- Page 30 4) Verify there is no lens breakage. 5) Retighten screws to 0.8 Nm (8 kgf·cm) eight hours later. 5.8.2 Component parts list for standard flow cell assembly Ref. Part Name Part Number Standard Remarks Gasket 228-35097-01 Lens(R4,RD6) 228-14572 Packing 228-14569 Retaining Screw, Lens 228-14568 SPD-16...
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Page 31: Other Functions
Sets ending wavelength of scan Numeric keypad ④ SCAN STEP Sets wavelength scan interval size Numeric keypad ⑤ PLOT SPD Sets speed of sending spectral data output to data Numeric keypad ⑥ recorder SPC PLOT Outputs spectral data to data recorder Enter ⑦ SPD-16... -
Page 32: System Group
② estimated replacement time PART Used to enter part replacement record. Numeric keypad ③ REPLACEMENT MAINTENANCE Displays maintenance log display only ④ OPERATION Displays operation log display only ⑤ ERROR LOG Displays error log display only ⑥ SPD-16... -
Page 33: Validation Support Group
Can be set when parameters sent using HDLC are IP ADRESS tenkey ⑳ enabled and DHCP server is not used SUBNET (same as above) tenkey MASK DEFAULT (same as above) tenkey GATEWAY TRS MODE RS-232C transfer mode tenkey SPD-16... -
Page 34: List Of Factory Functions
D2 FIL CUR Displays D2 filament current value (mA) display *⑳ Normal value: 700 ±100 only *21 D2 TRIG CT Displays D2 lamp trigger count display only *22 W FIL CUR Displays W filament current value (mA) display SPD-16... - Page 35 32768: Reverses “FUNC” logic (Normally startup -> special mode -> If "FUNC" is pushed and a power supply switch is turned on -> normal mode) *32 CLEAR ALL Initializes logs and backup memory Enter *33 PRODUCT Used to enter instrument type. tenkey TYPE 1: 16 SPD-16...
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Page 36: Cleaning The Temperature-Controlled Flow Cell
Screws A quartz window is located behind the spring. Pull the spring out slowly and remove the window. Wipe any dirt from the window using a Kimwipe, etc., and reassemble the parts. SPD-16... -
Page 37: Replacing Lamps
7.3 Adjusting the optical system 7.3.1 Adjustment overview Adjustment of models SPD-16 involves adjusting the D2 lamp, mirror M1, slit, mirror M2, grating, mirror M3 and cell, as shown below. The procedure for adjusting each component is explained below. SPD-16... -
Page 38: Preparations
Open the lamp housing and spectroscope room by loosening the screws on the top covers (4 screws on lamp housing and 5 screws on spectroscope room). 7.3.3 Optical adjustment jigs (targets) (Jig: 228-34982) Make a full size copy of the following and cut them out for use as targets. SPD-16... -
Page 39: Adjusting M1
Loosen screws “A” on the M1 assembly and rotate the assembly. Vertical adjustments to image on M2 jig: Adjust the angle of elevation using the screws “C”. Do not loosen set screws “B” and the nuts that secure them. After making adjustments, tighten screws “A”. SPD-16... -
Page 40: Adjusting Slit Position
Adjusting slit position 7.3.5 Attach the slit assembly and adjust it in the X-direction so the image center (the brightest portion) is projected on the slit, then secure the slit assembly SPD-16... -
Page 41: Adjusting M2
M3. Loosen the grating lock screw and rotate the grating in direction “B” in the figure. Secure the grating in location when the spectrum becomes horizontal. Readjust the vertical angle of assembly M2 so the image is vertically centered on the M3 jig and tighten the nut. SPD-16... -
Page 42: Adjusting M3
the 0-order light image hits the center of the target on the cell jig. Remove the M1 jig, attach a dummy cell, and double check that the long narrow 0-order light image is over the cell hole. SPD-16... -
Page 43: Check The Position
Turn on the power while pressing the “Func” key. Use the “FILTER POSI” function from factory functions group to display the filter position and set it to “0”. Check that the filter semi assembly is not blocking any part of the slit. SPD-16... -
Page 44: Additional Explanations
7.4 Additional explanations This service manual refers to special mode functions (power is turned on while pressing the “Func” key), but this mode is reserved for Shimadzu service engineers. To avoid confusion, do not reveal this mode to customers. 1 Differences from previous models 7.4. -
Page 45: Additional Vp Functions
Correctly enter the first 12 characters, but the last two characters (an alphanumeric code indicating the manufacturing plant) do not need to be entered. Example: L21004101234J9 = L2 100 41 01234 J9 L2: LC model type 100: model # 41: Production time 01234: sequential # J9: factory SPD-16... - Page 46 None of the log records can be deleted. (4) Date and time Each log records the date and time of each event, but model SPD-16 does not have a backup clock, so the expression of date and time may differ depending on the system status.
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Page 47: Compatibility Of D2 Lamps With Previous Models
3 Compatibility of D2 lamps with previous models 7.4. D2 lamps for SPD-10A and SPD-M10Avp models can be used in SPD-16 systems. The inter compatibility of D2 lamps is shown below. In addition, D2 lamps for model SPD-16 are compatible with SPD-10Avp systems. Instrument SPD-10A... -
Page 48: Voltage At Data Recorder Output Terminal
4 Voltage at data recorder output terminal 7.4. The recorder output terminal voltage for model SPD-16 is 10 mV. If the recorder input full scale is 1 mV, use one of the following methods to change the full scale level. - Page 49 ⑤ Once the update is complete, the computer will display “ROM UPDATE IS COMPLETED”. ⑥ The instrument display will not change even after the update has been completed. Briefly turn off the power and restart the instrument. SPD-16...
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Page 50: Troubleshooting
Key operated during KEY CLOSE mode Warning → Press Del+CE to cancel KEY CLOSE ⑱ DATA NOT EXIST Spectrum outputted without scanning→ Perform scan Warning ⑲ NO D2 LAMP D2 lamp not installed Alarm → D2 lamp disconnected or defective, PCB defect SPD-16... -
Page 51: Problems With Electrical Circuits And Points To Check
M12, M13, M19, J19 D2 or W lamp current values J19, M19, R32, R33, R39, abnormal D5 -- D8 Hg lamp does not turn on/off M1, J1, Q1, C3 J2 connector output voltage for PWR PCB J19, M12, M19 not changing SPD-16... - Page 52 M34, R50, R102, R103, RADIATOR T = 530 “RADIATOR T” ±30 R105, D22 values ⑧ Contact output and event input circuits Item Allowable range Points to check Time program does not M14, M32, K1, K2, J16, stop R70, R71, F18 -- F21 SPD-16...
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Page 53: Spd20-Pwr(Power) Circuit Board
700 ±100 R/C around M8, R47, M8 FIL CUR] value W lamp does not R/C around M2, M3, M5, Q4, go on/off L1, D11, R32, R33,R19, M2 Abnormal [W FIL 1500 ±200 R/C around M2, R19, M2 CUR] value SPD-16... - Page 54 ④ Cooling fan control circuit Item Allowable Points to check range M12, J7, J9 Cannot control ⑤ Flow cell temperature control circuit Item Points to check Allowable range Abnormal temp 40 ±1°C R/C around M2, M13, Q7, J4, R65, display values M2/M13 SPD-16...
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Page 55: Maintenance Parts Lists
228-42008-91 Filter ASSY (UV) 2nd-order light cut filter 228-23027-92 Motor, GR ASSY Grating drive motor 228-25421-94 Photosensor A ASSY Sensor for mirror switching 228-34795 Window Quartz window pane 228-23016-91 Silicon photocell Silicon ASSY photodiode 228-23691-95 PB-1 SPD20A ASSY Pre-amplifier SPD-16... -
Page 56: Electrical Parts List
228-42098-92 Rear fan MD516B fan ASSY 228-39247-95 Leakage sensor Leak sensor ASSY 075-00070-87 with input voltage Filter, FN378-4151 selector 465-04115-92 Indicate state of Indicator light detector 465-04205-92 PCB SUB ASSY 9.4 Optional Parts 9.4.1 Inert cell (without temperature control) SPD-16... - Page 57 020-46535 Screw, SST sems P4 M3 x 6 020-46634 228-23424 Cover, cell 228-24181 Retaining bush Cover 228-32985 Screw, SST pan head 020-02906 M2 x 4 228-33255 Sponge 228-31638 Ferrule 1.6F PEEK FEP tube, NFL016 016-37722-06 FEP tube, NFL016 016-37722-06 SPD-16...
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Page 58: Preparative Cell
228-21385- Gasket 228-00147-02 Window, UV cell 228-21387 Gasket, cell window bracket 228-21386 Screw, cell window fixing 020-46548 Screw, SS sems P3 M4 x 10 020-46634 Screw, SS sems P4 M3 x 6 021-04633 Screw S WP M4 x 5 SPD-16... -
Page 59: Parts List And Diagram For Solvent Recycle Valve Control
Screw, SS truss head M4 x 8 020-46536 Screw, SS sems P3 M3 x 10 072-60319-01 Clamp, DKN-10GSP 228-35453 Cover, SV 075-08032-03 Filter core, EMI SFC-6 228-35673 Tray 228-35674-01 Spacer 228-35674-02 Spacer 228-30292 IM, recycle valve kit (JE) 037-02797-01 Knob, KDM-16-M4-10 228-25162-03 Tube, drain SI SPD-16... - Page 60 This page is intentionally left blank. SPD-16...
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