Related Manuals for AmScope T720 Series
Summary of Contents for AmScope T720 Series
- Page 1 T720 SERIES OPERATOR MANUAL (Please Read This Manual Before Using the Microscope) T720 Series Compound Microscope...
- Page 2 AmScope, and is protected by laws pertaining to such materials. This document, the information in this document and all rights thereto are the sole and exclusive property of AmScope and are not to be copied, used or disclosed to anyone, in whole or in part, without the express written permission of AmScope.
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Page 3: Table Of Contents
T720 Series Microscope Contents BEFORE USE................................4 Introduction .................................4 Precautions ................................4 1. M ...............................5 ICROSCOPE ARTS 2. O ................................7 PERATION 2.1 Unpacking ..............................7 2.2 Assembly ................................8 2.3 Adjusting the View ............................11 2.4 Specimen Set Up............................12 2.5 Focusing...............................13 2.6 Using the Trinocular Port ..........................14 2.7 Attaching a Camera............................14... -
Page 4: Before Use
Congratulations on the purchase of your new AmScope Microscope! This manual is designed for T720 series microscopes. Though the microscope’s features may vary depending on your series model number, the T720 series functions remain the same. Please take a few minutes to familiarize yourself with the features and functions of your new microscope. -
Page 5: Microscope Parts
T720 Series Microscope 1. Microscope Parts 1.1 Parts Diagram AmScope Proprietary Page 5 of 46... - Page 6 T720 Series Microscope 1.2 Features This brand new professional trinocular compound microscope comes equipped with an infinity plan optical system and offers advanced features as well as research grade performance. Its crystal clear optical and precise mechanical systems promise high quality performances for advanced applications.
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Page 7: Operation
T720 Series Microscope 2. Operation 2.1 Unpacking 2.1.1 Unpack the Box 1. Very carefully slide the Styrofoam container out of the cardboard carton. 2. Lay the Styrofoam container on its side. Make sure the side labeled up is up. 3. Remove the tape. -
Page 8: Assembly
T720 Series Microscope 2.2 Assembly 2.2.1 Carefully Remove Microscope from Box Remove the microscope body from the box and remove the plastic protective covering. The body of the microscope is composed of the base, the stage, the arm, and the nosepiece. - Page 9 T720 Series Microscope 2.2.4 Install the Trinocular Port and C-Mount Unscrew and remove the photo port cap from the top side of the microscope head. Screw in the Trinocular port. If your camera has a c-mount, insert the c-mount adapter onto the Trinocular port.
- Page 10 T720 Series Microscope 2.2.6 Insert Objective Lenses Unpack the objective lenses. Screw the objectives into the microscope nosepiece. Start with the lowest magnification, and in order of magnification, insert each lens, without touching the glass. 2.2.7 Plug it in, turn it on, and adjust the dimmer if more light is needed.
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Page 11: Adjusting The View
T720 Series Microscope 2.3 Adjusting the View 2.3.1 Choose forward or reverse viewing The microscope head rotates to allow for forward or reverse viewing. There is no difference as far as the optics; it depends on what’s comfortable. In forward position you are looking over the stage, in reverse position you are looking at the stage. -
Page 12: Specimen Set Up
T720 Series Microscope 2.4 Specimen Set Up 2.4.1 Prepare the Specimen Place the specimen to be studied on a glass slide (or use a prepared slide, purchase separately). 2.4.2 Secure the Slide Place the slide on the stage, holding it snugly in place with the metal slide holders (clips) of the mechanical stage. -
Page 13: Focusing
T720 Series Microscope 2.5 Focusing 2.5.1 Choose an Objective Lens Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4X objective) to locate and focus on the specimen. As you move up in magnification you will need to refocus the image a little each time. -
Page 14: Using The Trinocular Port
T720 Series Microscope 2.6 Using the Trinocular Port The AmScope T720 model is uniquely designed so that you can view the image through the eyepieces and the Trinocular port simultaneously, as well as fine tune the focus of the camera with a C-mount focus adjustment. -
Page 15: Setting The Stage's Stop-Limit
T720 Series Microscope 2.8 Setting the Stage’s Stop-Limit 1. Unlock the stop-limit on the stage. 2. Adjust the stage to the desired maximum height. 3. Lock the stop-limit. This allows you to limit the movement of the stage from the bottom of the range up to the point it is set at. -
Page 16: Adjusting The Iris
T720 Series Microscope 2.11 Adjusting the Iris By changing the aperture (hole size) of the iris/diaphragm of the condenser, you can adjust the background brightness. Adjust the aperture of the iris diaphragm using the numbered iris adjustment ring located directly under the stage. -
Page 17: Using Oil Immersion
T720 Series Microscope 2.14 Using Oil Immersion When using the 100X objective, a drop of immersion oil should be placed between the cover slip and the objective to minimize distortion caused by air. 1. Place the slide on the stage and center the specimen. - Page 18 T720 Series Microscope 7. Re-engage the stage stop limit. 8. When the specimen is in focus, adjust the iris to open just larger than the condenser’s diameter. 9. After using the oil immersion lens, you will need to clean the lens and specimen before the oil dries.
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Page 19: Using Darkfield Condenser Lenses (Optional Accessories)
T720 Series Microscope 2.15 Using Darkfield Condenser Lenses (Optional Accessories) Darkfield Illumination is a lighting method for increasing contrast. A darkfield condenser lens produces bright images against a dark background for low contrast biological specimens. The dry darkfield condenser is for low power magnifications, such as with the 4X, 10X or 40X objective lenses, with numeric apertures of .65 or less. -
Page 20: Using A Dry Darkfield Condenser Lens (Optional Accessory)
T720 Series Microscope 2.16 Using a Dry Darkfield Condenser Lens (Optional Accessory) 2.16.1 Using the Dry Darkfield Condenser 1. The first step is centering the condenser. Move the 10X objective lens into the light path. 2. Remove an eyepiece and look through the empty eye tube, to see the condenser’s image directly. -
Page 21: Using Oil Darkfield Condenser Lens (Optional Accessory)
T720 Series Microscope 2.17 Using Oil Darkfield Condenser Lens (Optional Accessory) The condenser works with 100X oil objective and low power objectives (4X, 10X, etc) as well. Immersion oil is needed on top of the condenser lens for darkfield observation. Immersion oil is needed between the 100X oil objective and slide. - Page 22 T720 Series Microscope 2.17.2 Using Oil Immersion 1. Before you install the oil darkfield condenser lens, apply a drop of immersion oil on the top lens of condenser. If you’ve already installed it, remove the condenser to apply the drop of oil to the lens, and re-install it.
- Page 23 T720 Series Microscope 2.17.3 Cleaning the Oil Darkfield Condenser 1. When using oil immersion, once you have finished using the oil darkfield lens, you will need to clean the lens and specimen of the oil before it dries. 2. You can wipe the lens with a soft nonabrasive cloth to remove the oil. If you want to use a cleaner, you can use cigarette lighter fluid to safely remove the oil, as it will break it down.
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Page 24: Microscope Maintenance
T720 Series Microscope 2.18 Microscope Maintenance 2.18.1 Handle with Care As the microscope and all accessories are precision instruments, always handle them with care, avoiding impact or abrupt movement during transportation. Do not shake the package. 2.18.2 No Sun/Heat Do not place the microscope and accessories in direct sunlight or in high heat. -
Page 25: Darkfield Condenser Kit Maintenance
T720 Series Microscope 2.18.8 Do not take apart the microscope. Do not assemble or disassemble the microscope’s electrical components yourself. 2.19 Darkfield Condenser Kit Maintenance 2.19.1 Secure Setting Ensure that the microscope is located on a smooth, level and firm surface. -
Page 26: General Specifications
T720 Series Microscope 3. General Specifications 3.1 Features FEATURE SPECIFICATION Optical System Infinity corrected Nosepiece Reversed, ball bearing quintuple Head Gemel type trinocular head, 30-degree inclined Infinity Plan Objectives 4X, 10X, 20X, 40X (spring), 100X (spring, oil) Focusing Low position coaxial focus system Interpupillary Adjustment Range 2-3/16"... -
Page 27: Objectives
T720 Series Microscope 3.4 Objectives Numerical Aperture Magnification Model (N.A.) Marks Type Number Magnification Rating Medium (Color Ring) PA4X Plan 4X 0.10 PA10X Plan 10X 0.25 Yellow Plan Objective PA20X Plan 20X (195mm) PA40X Plan 40X 0.65 Light Blue PA100X Plan 100X 1.25... -
Page 28: Parameters
T720 Series Microscope 4. Parameters 4.1 Electrical System 90V-240 Wide Voltage, CE Certified 4.2 Microscope Parameters Magnification 10x-2500x Field of View Φ0.8mm~Φ4.5mm Mechanical Tube Length 160mm Object to Primary Image Distance 195mm Fine Focusing Sensitivity 0.002mm AmScope Proprietary Page 28 of 46... -
Page 29: Recommended Accessories
T720 Series Microscope 5. Recommended Accessories (Purchase separately. Please visit www.amscope.com and search with SKU #, for more information.) 5.1 Eyepieces 5X Eyepieces 20X Eyepieces 25X Eyepieces SKU: EP5X23 SKU: EP20X23 SKU: EP25X23 10X w/ Pointer 10X w/ Reticle SKU: EP10X23P SKU: EP10X23R 5.2 Darkfield Condensers... -
Page 30: Objective Lenses
T720 Series Microscope 5.3 Objective Lenses Achromatic SKU: A2X SKU: A5X SKU: A20X SKU: A60X Plan Achromatic Plan 4X Plan 10X Plan 40X Plan 100X SKU: PA4X SKU: PA10X SKU: PA40X SKU: PA100X 5.4 Fuses Fuses For Microscopes SKU: FS-M... -
Page 31: Cameras And Accessories
T720 Series Microscope 5.5 Cameras and Accessories Cameras To capture images, video, or view live display on a computer (PC/Mac OS X). Real- Time Live Video Microscope Digital Camera, Reduction Lens, High Sensitivity Sensor and Deluxe Calibration Kit. 350K 1.3 Mega... -
Page 32: Slides
T720 Series Microscope 5.6 Slides Prepared 25 Slides 50 Slides 100 Slides 200 Slides SKU: PS25W SKU: PS50 SKU: PS100A SKU: PS200 Blank 50 Blank Slides 100 Blank Slides 144 Blank Slides 300 Blank Slides SKU: BS-100P- SKU: BS-144P- SKU: BS-300P-... -
Page 33: Troubleshooting
T720 Series Microscope 6. Troubleshooting 6.1 Optical Issues SYMPTOM SYMPTOM CAUSE REMEDY OPTICAL ISSUES Turn the nosepiece until it clicks into The nosepiece is misaligned. place. One side of the field of view is Stains, dust, or dirt has darker... -
Page 34: Mechanical Issues
T720 Series Microscope SYMPTOM CAUSE REMEDY OPTICAL ISSUES Secure the slide to the stage with One side of the field of The specimen slide is not fixed. clips. view is dark or the image The nosepiece is not in the right... -
Page 35: Electrical Issues
T720 Series Microscope 6.3 Electrical Issues SYMPTOM CAUSE REMEDY ELECTRICAL ISSUES Replace the bulb. This unit uses our BH- The bulb/light source The bulb is close to 6V20W with our 20w unit or our BH- flickers. burning out. 6V30W for the 30W unit. -
Page 36: General Microscopy Guide
7. General Microscopy Guide Microscopes come in a wide variety of types with many different features. Each AmScope model is designed for specific uses and specific users. This glossary will help illustrate what the variations mean and why they are useful. - Page 37 T720 Series Microscope Base Lens Base lenses direct the light source towards the specimen. Binocular Head-Rotating A rotating binocular head is designed for maximum flexibility in viewing options. Binocular microscope heads allow for the easy adjustment of the interpupillary distance...
- Page 38 T720 Series Microscope Condenser Lens Adjustment Knob The Condenser Lens Adjustment Knob changes the distance between the light condenser and the base lens. This allows you to control the concentration of the light hitting your slide. Darkfield Condenser Lens A darkfield condenser lens produces bright images against a dark background for low contrast biological specimens.
- Page 39 T720 Series Microscope Diopters Small rings on the eyetubes, used to focus ocular lenses (eyepieces). Eye Guards Eye Guards fit over the eyepieces, they are for comfort and to protect the glass in the eyepiece. Eyepieces Eyepieces are also called ocular lenses. Eyepieces come in many magnifications and you replace them by swapping them out of the eyetubes.
- Page 40 T720 Series Microscope Field of View and Working Distance Field of view is how much of the specimen you can see through the eyepiece. The linear field of view of the eyepiece is divided by the magnification of the objective.
- Page 41 T720 Series Microscope Head Types Monocular Monocular microscope heads have one eyepiece. Binocular Binocular microscope heads have two eyepieces. Trinocular Trinocular microscope heads have two eyepieces and a photo port for a camera. This allows you to look at a specimen with a camera without removing an eyepiece.
- Page 42 Interpupillary Distance Interpupillary Distance is the distance between your eyes. AmScope uses 2 types of systems to change interpupillary distance. Siedentopf heads adjust the interpupillary distance by rotating the eyetubes around a central axis.
- Page 43 When changing light bulbs cover skin with cloth or paper to handle the bulb. This will protect both you and the bulb. 3. Hot bulbs will not damage the microscope. AmScope’s microscopes are designed specifically to handle the heat output of the model’s bulb.
- Page 44 T720 Series Microscope Limit Stop Knob The limit stop is designed to prevent impact between objective and slide. You may loosen the limit-stop knob in order to give yourself the full range of motion for fine tuning the focus when using the 40X and 100X objective lenses.
- Page 45 T720 Series Microscope Nosepiece This circular structure is where the different objective lenses are screwed in. To change the magnification power, rotate the turret. Numerical Aperture (N.A.) N.A. is a rating for the resolution of the objective lens. The N.A. rating ranks the objective lens’...
- Page 46 T720 Series Microscope Trinocular Port A trinocular microscope has three viewing ports. There are two eyepieces and a third port for photography and video. The trinocular C-Mount adapter. AmScope Proprietary Page 46 of 46...
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