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Product Manual for ProPac
Figure 3 shows the separation of hemoglobin A2 using both ProPac SCX-10 and ProPac SCX-20 columns. A faster
separation could be achieved on ProPac SCX-20 within 16 minutes with a different selectivity and better resolution of the
main peak.
80.0
60.0
40.0
20.0
-10.0
mAU
45.0
25.0
12.5
-5.0
0.0
10.0
Separation of Hemoglobin A2 on ProPac SCX-10 and ProPac SCX-20 columns
Document No. 065403-01
®
SCX-20
ProPac SCX-20
ProPac SCX-10
20.0
Minutes
©2010 DIONEX CORPORATION
Column:
Eluent A :
Eluent B:
Gradient:
Flow Rate:
Inj. Volume: 5 µL
Temperature: 30 °C
Detection:
Samples:
30.0
35.0
Figure 3
4 x 250 mm
50 mM Sodium Phosphate pH 6.0
+ 2mM KCN
0.5M NaCl in Eluent A
3-12% B in 12 min; to 40% B in 30 min
1 mL/ min
UV, 280 nm
Hemoglobin A2
(Helena Laboratories)
September 2010
Page 10 of 21
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