Related Manuals for VWR avantor 49893-2000
Summary of Contents for VWR avantor 49893-2000
- Page 1 Instruction manual Automated Cell Counter Fluo ® EU cat. no 49893-2000 Version 01 Issue 03/2023...
- Page 2 VWR International bv, Researchpark Haasrode 2020 Geldenaaksebaan 464 B-3001 Leuven +3216385011 be.vwr.com UK Importer: VWR International Ltd Hunter Boulevard, Magna Park Lutterworth, Leicestershire, LE17 4XN uk.vwr.com Country of Origin China vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
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Page 3: Table Of Contents
TECHNICAL SERVICE ............................. 32 WARRANTY ................................32 COMPLIANCE WITH LOCAL LAWS AND REGULATIONS................32 EQUIPMENT DISPOSAL ..............................33 APPENDIX — OUR LOCAL OFFICES IN EUROPE, AMEA AND NORTH AMERICA..........34 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 4: 1- Safety Information
In case the instrument breaks down or requires any service, shut down the instrument and contact with VWR customer service. 1.2 Electrical safety The following provisions should be followed for the safe use of the electrical devices accompanied with the instrument: a) Never disassemble the instrument without any authorization against any possible electric shock. -
Page 5: Biosafety
Complies with the requirements of the EC EMC directive 2014/30/EU with amendments. Test Standards: EN IEC 61326-1: 2021 EN IEC 61326-2-6: 2021 EN IEC 61000-3-2: 2019/A1: 2021 EN 61000-3-3: 2013/A2: 2021 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 6: 2- Package Contents
This equipment is designed for measurement and further fluorescence analysis of cell quantity and viability for cell lines, stem cells and primary cell samples. For research only. Not intended for diagnostics use. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 7: 4- Symbols And Conventions
I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 8: 5- Product Specifications
CV<5% Built-in memory 1,000 entries Touch screen resolution 1024*600 Operation response time 100 ms Optics 5 megapixel camera, 2.5X optical magnification DAPI(375/28Ex 460/50Em) Fluorescence cube (optional) AO/GFP(480/30Ex 535/40Em) PI(540/25Ex 620/60Em) vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 9: 6- Instructions For Use
⑨ Power input: users must use provided power cord and proper plug in compatible with the socket in user’s country to connect the instrument to the socket by the power input. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 10: 7- Operation
Click [Profiles] on the main interface to enter the profiles setting interface, with the instrument in Non- Trypan Blue Mode (Non-TP) by default under which the total cell concentration, histogram of total cell size distribution histogram and average size are available for view. Fig. 7-2 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... - Page 11 Note: if the BF channel is only selected on the Count interface in the FL mode, only the total cells are available for calculation, and fluorescent cell will be undistinguishable. Fig. 7-4 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 12 The circularity slider can only be used to set a single threshold value; cells within the set value range will be counted, excluding the cells beyond the range. Drag the slider toward the target to adjust the circularity threshold. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 13 Click the [Help] symbol in the upper right corner of the interface to learn more about the concepts of size, brightness, circularity, RFU and so on as shown in figure 7-6. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 14 Note: Autosaving succeeds only when the local storage is sufficient. Restore to Default Mode Click [Default] to restore part of the initial settings, including Auto FL Exposure, Auto Exposure, Quick Mode, Standard Mode, threshold value of each profiles, etc. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
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Page 15: Profiles Loading
Cell concentration range: 1 x 10 –1 x 10 cells/mL, optimally 1 x 10 –4 x 10 cells/mL. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 16: Methods Of Staining
Note: since DAPI stain solution is semi-permeable and can stain both live cells and fixed cells, longer staining time for live cells is required, otherwise the proportion of stained cells will be lower. For vwr.com I Instruction manual VWR ®... -
Page 17: Loading Samples Into Slide
After counting, just push the counting slide gently into the slide port until it clicks and is pushed out under spring pressure. Hold the counting slide by the edges to take it out. Note: do not reuse the disposable counting slide, which may influence the count results. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 18: Bright Field Assays
For setting the dilution factor of the sample. Click“1×”to pop up a numeric keypad. The range of setting value: 1.00~999.00. Note: The default dilution of Trypan Blue mode is “2×”, and the default dilution of "AO+PI" mode is “2×”. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... - Page 19 The overexposed, moderate and underexposed effects of the bright field are as shown in Fig. 7-10 (A-C) below. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 20 It is critical to ensure proper focus to accurately identify cells and distinguish dead or live cells. Cell images with correct and incorrect focus are as shown in Figure 7-11(A-B) vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 21 Upon completion of operations on the [Count] page, the [Result] page will pop up, where users are allowed to zoom in, mark cells, or view histograms, adjust profiles and count for the captured images. The results page of TP Mode as shown below. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 22 Fig. 7-13 Click to mark cells, as shown in the Fig.7-14 below. Fig. 7-14 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
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Page 23: Fluorescence Assays
Note: for the icons and their functions in FL assays, please refer to the corresponding introductions of BF assays. Click to switch to the data acquisition page of FL assays Fig. 7-16 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... - Page 24 Since the application of fluorescence is complex, the result of auto FL exposure is affected by the RFU and the quantity of fluorescent cells. The result of the overexposure, moderate and underexposure of fluorescence is as shown in Figure 7-17(A-C) Fig. 7-17 vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 25 Take AO & PI for an example: Results page of [Channel] Fig. 7-18 Results page of [Application] Fig. 7-19 is a channel button; the channel menu will pop up when it is clicked. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
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Page 26: Results Storage
Note: 1000 entries are available for storage in the system at most. If users want to save new data beyond this limit, the earliest count results saved will be deleted by default. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 27: Previous Counts
Click [System Settings] on the Homepage to enter the following interface. It allows users to perform such actions as software update, date / time setting, language switching, fluorescence cube resetting (for replacement of fluorescence cube). Note: “Maintenance” is only for after-sales personnel. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... - Page 28 After the reset is successful, please replace the fluorescent column when turns off the power switch and disconnects the device power. 2) Switch off the power switch and power off the device and immediately replace the cube. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
- Page 29 6) Install the prepared fluorescent cube in the original position and lock it tightly to complete the installation. Install the other cube with the same method. vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo...
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Page 30: 8- Troubleshooting
FL mode will be disabled” services or visit www.vwr.com pops up Alarm prompt “Camera error, please Power off the device and contact local Avantor contact after-sales personnel!” services or visit www.vwr.com vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 31: 9- General Maintenance
User replaceable accessories and spare parts Description Quantity Cat. No. VWR® Fluo Cube Green GFP 49893-4951 VWR® Fluo Cube Red RFP / PI 49893-4952 VWR® Fluo Cube Blue DAPI 49893-4950 AC adapter VWR® Cell counting slides 1 box of 50 pcs 10228-0050 vwr.com I Instruction manual VWR... -
Page 32: 11- Technical Service
VWR warrants that this product will be free from defects in material and workmanship for a period of two (2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period. -
Page 33: Equipment Disposal
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you vwr.com I Instruction manual VWR ® Automated Cell Counter Fluo... -
Page 34: Appendix - Our Local Offices In Europe, Amea And North America
VWR International A/S VWR International GmbH Tobaksvejen 21 Mexico Lerzenstrasse 16/18 2860 Søborg VWR International, S.de R.L. de C.V. 8953 Dietikon Tel.: +45 43 86 87 88 Km. 14.5 Carretera Tel.: +41 044 745 13 13 Email: info.dk@vwr.com Tlalnepantla-Cuautitlán Email: info.ch@vwr.com...
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