Related Manuals for Radleys Carousel Work-Up Station
Summary of Contents for Radleys Carousel Work-Up Station
- Page 1 February 2023 | Issue 5 Instructions Carousel Work-Up Station ™ Cat No. RR99830 Your Local Distributor Radleys Shire Hill, Saffron Walden, Essex, CB11 3AZ. United Kingdom. Tel: +44 1799 513320 Email: sales@radleys.co Web: www.radleys.com...
- Page 2 RR99830 - Carousel Work-Up Station Index Page 1 Index Page 2 Introduction Warranty Page 3 Safety Information Important WARNINGS Page 4 Products & Accessories Page 5 Carousel Work-Up Set-Up & Operation Page 6 Using the SpeediFlow Booster Page 7 Using the SpeediFlow Booster - continued...
-
Page 3: Warranty
The lower rack supporting 12 corresponding Carousel Reaction Tubes or standard 1 inch boiling tubes for subsequent sample collection. The Carousel Work-Up Station allows up to two levels of 70ml columns stacked one above the other. This enables parallel and/or sequential work-up in 12 samples, using filtration, phase separation or solid phase extraction techniques. -
Page 4: Safety Information
Important, Important, Important, Important, Important WARNINGS Please read these instructions completely before using your Carousel Work-Up Station Only operate in a fume cupboard with protective safety sash. When using chemicals and reagents always follow appropriate safety instructions and chemical handling procedures. -
Page 5: Products & Accessories
RR99877 Liquid/Liquid Extraction, 20ml Sample, 70ml RR99878 SpeediFlow Gas Connection Tubing - 5m length RR99840 Carousel Work-Up Station Systems:- include all the important components and accessories required for efficient operation Cat No Description Pk Qty RR99876 - Carousel Work-Up Column Pack... - Page 6 RR99830 - Carousel Work-Up Station Carousel Work Up Set-up & Operation The Carousel Work-Up Station consists of two identical racks that stack on top of each other. The racks lock together with a pin so that the stack of two racks can be easily carried or moved safely and securely.
- Page 7 RR99830 - Carousel Work-Up Station Using the SpeediFlow Booster The SpeediFlow is used to apply top (positive) pressure to Filtration or SPE Columns. Flow rates through these columns (particularly SPE) can be slow with- out additional pressure being applied either via vacuum or top pressure. Pressure...
- Page 8 RR99830 - Carousel Work-Up Station Using the SpeediFlow Booster - continued 5. If you want to leave the SpeediFlow unsupported then press the thumb button when the trigger lever is fully compressed. This will lock the trigger into position and and allow a constant pressure to be maintained in the column without holding the trigger.
- Page 9 RR99830 - Carousel Work-Up Station The SpeediFlow is supplied with a Viton O-ring as standard. ® An optional Perlast O-ring is available for use with more aggressive solvents. ® Perlast is a perfluoroelastomer (similar to PTFE) that offers excellent resistance to a broad range of commonly used chemicals and exhibits minimal swelling even with prolonged exposure to vapours.
- Page 10 RR99830 - Carousel Work-Up Station Choice of Columns The Carousel Work-Up Station is designed to accept 70ml tubes and columns which is the most appropriate size for most work-ups on the Carousel Reaction Station scale. The choice of column depends on many factors including: •...
- Page 11 RR99830 - Carousel Work-Up Station Sample Preparation Prior to SPE Purification Prior to purification by SPE (Solid Phase Extraction) the crude reaction mixtures, from for example a parallel set of reactions in the Carousel Reaction Station, may need to be worked up.
- Page 12 RR99830 - Carousel Work-Up Station Using Phase Separation Columns The Phase Separation Columns contain a hydrophobic frit and are designed to separate organic phases that are denser than water (e.g. dichloromethane). They can also be used for sequential purification, e.g. phase separation followed by SPE.
- Page 13 RR99830 - Carousel Work-Up Station Using Liquid/liquid Extraction Columns The Liquid/liquid Extraction Columns contain a modified diatomaceous earth which efficiently absorbs water from aqueous samples. The diatomaceous earth is chemically inert and stable over the ph range 1-13. Unlike the Phase Separation Columns the Liquid/liquid Extraction Columns can be used with non-water miscible solvents that are either more or less dense than water.
- Page 14 RR99830 - Carousel Work-Up Station Selecting the Correct SPE Column RDT offer four types of SPE column that will cover the vast majority of purifications for the Carousel Work-Up Station. These are: Cat No Description Volume Pk Qty RR99870 Silica 10g/70ml...
- Page 15 RR99830 - Carousel Work-Up Station Guidelines for Using SPE Columns The overall purification process normally consists of 5 steps: 1. Sample Preparation – initial work-up See above for guidelines on the use of Filtration, Phase Separation and Liquid/liquid Extraction Columns.
- Page 16 RR99830 - Carousel Work-Up Station Guidelines for Using SPE Columns - continued 4. Elution of impurities Impurities are normally eluted using the same solvent as is used for solvating the column. Use the Speediflow to provide a positive pressure to increase flow rates.
- Page 17 Page 16...