GenScript eZwest Lite Manual
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GenScript eZwest Lite Manual

Automated western device
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eZwest
Lite
TM
Automated Western Device
Start Your Easy Western from Here

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Summary of Contents for GenScript eZwest Lite

  • Page 1 eZwest Lite Automated Western Device Start Your Easy Western from Here...

  • Page 3: Table Of Contents

    Version 20220615 Table of Contents Components and Initial setup Warranty 1. Instrument Overview 1.1 Instrument overview 1.2 Specifications 1.3 Ordering information 1.4 Safety & Maintenance 2. Instructions 2.1 Instrument display and feature location 2.2 Basic operation Save cassette ID when using the cassette for the first time View and modify methods Set cassette specific methods 2.3 Using the Pre-programmed blotting method...

  • Page 4: Components And Initial Setup

    Components and Initial setup Important! eZwest Lite Automated Please check that all parts listed below are included with your package. Western Device Components Cat. Size Quantity eZwest Lite Automated Western Device L00816 1 unit eZwest Membrane Cassette L00820 1 unit eZwest Cleaning Cassette L00821 1 unit...

  • Page 5: Warranty

    • Repair or modification done by any other party than GenScript or an authorized agent. • Use of fittings or other spare parts supplied by any other party than GenScript. • Damages caused by disasters. For consultation and maintenance services, please contact GenScript’s customer service and provide the following information.

  • Page 6: Instrument Overview

    100-120 V or 220-240 V，50/60 Hz Operating Temperature： 15-40°C, 59-104°F Max Membrane Size(WxH)： 90x83 mm, 3.5x3.2 in Throughput： 1 channel for up to 2 membranes Application： Western blotting eZwest Lite： ABS，PP，stainless steel，silicone Forceps： Roller： Stainless steel, POM - 3 -...

  • Page 7: Ordering Information

    1.3 Ordering information eZwest Membrane Cassette Size Quantity Cat. Product (included in L00816) 1 unit L00820 eZwest Membrane Cassette Accessories and other Product Size Quantity Cat. reagents eZwest Cleaning Cassette 1 unit L00821 Membrane Spacer 50 pcs L00824 Stylus Pen 1 pc L00822 1.4 Safety &...

  • Page 8: Instructions

    2. Instructions 2.1 Instrument display and feature location eZwest Lite automated western device Touch screen Stylus pen Front view Front cover Reagent chamber Fig. 1 Back view Waste 1 Waste 2 System ON/OFF switch Wash buffer AC power receptacle Fig. 2 Top view Blocking buffer...
  • Page 9 Membrane cassette Latch Buffer ports Sensor chip Fig. 4 Current program name Screen display (main menu) System menu Method menu Program timer Current program steps Start program Fig. 5 Screen display (Method view) Exit Select program Save program Program steps Device programs System clean Cassette program...

  • Page 10: Basic Operation

    3. After the cassette is inserted, you will be asked if you want to “save ID”. 4. Press OK to save cassette ID as show in Fig. 8. Note: Each eZwest Lite device could save up to 5 cassette IDs. Fig. 8 View and modify methods 1.
  • Page 11 2. Select method on the left to show the steps of each method. Fig. 10 3. Select a step to show and modify the associated details. Note: Each Method may be programmed with 6 steps. Fig. 11 4. Each step within a Method is customizable : a.

  • Page 12: Set Cassette Specific Methods

    c. Run time for each cycle in minutes. Fig.14 d. Pump Speed for recirculating the liquid flow through the membrane cassette. Fig.15 5. Once the method is set up, press “Save” Set cassette specific methods The eZwest Lite device can store up to one dedicated method for each cassette. Upon cassette installation, select the associated method.

  • Page 13: Using The Pre-Programmed Blotting Method

    2.3 Using the Pre-programmed blotting method 1. Prepare blotting solutions a. Blocking buffer, 10 mL b. Primary antibody solution, 10 mL c. Secondary antibody solution, 10 mL d. Wash buffer, 10 mL per wash cycle, e.g. 90 mL if wash 3 times and each with 3 cycles 2.
  • Page 14 4. Placing membrane(s) in the casette a. Processing a single membrane: Place the membrane in the center of the cassette surface as shown below in Fig. 20(i), with the protein side facing up and the high MW side aligning towards the bottom edge of the cassette as Fig.

  • Page 15: Sample Protocols

    3. Sample Protocols 3.1 Quick Protocol: Requires sensitive antibodies and/or higher abundant proteins. All steps performed at room temperature. Step Cycles Time Pump speed Blocking 30min Slow Wash 5min Normal 1°Antibody 60min Slow Wash 5min Normal 2°Antibody 30min Slow Wash 5min Normal 3.2 Normal Protocol:...
  • Page 16 2. Select the cassette ID (e.g., “345”) to enter the cassette program. Fig. 23 3. Select the steps for customization (overnight option is only available to Blocking, 1°Ab, and 2°Ab steps). Fig. 24 4. Open the dropdown menu for “Cycles” and select “Overnight”. The same setting can be applied to 1°Ab or 2°Ab steps.
  • Page 17 8. Check buffer volumes for each required reagent. E.g., if select Overnight Cycle under the 1°Ab step, only Wash buffer, Blocking buffer and 1°Ab solution are required to start the overnight protocol in the eZwest device. Fig. 28 9. After 1° Ab incubation step is finished in eZwest, the machine will automatically pause with a notice as shown in Fig.
  • Page 18 14. Replace the blocking buffer with 20mL wash buffer and leave antibody tubes empty for the cleaning cycle. Check the buffer volumes and start system cleaning. Fig. 33 Fig. 34 15. Put the overnight cassette back into eZwest. The device will recognize the unfinished overnight protocol and request to continue.

  • Page 19: Troubleshooting And Faqs

    Replace cassette/ insert cassette cassette No Cassette Cassette not secured in Check Pins and Reinsert position cassette securely Pump Error Pump malfunction Contact GenScript System Error System malfunction Contact GenScript 4.2 FAQ Description Possible causes Solutions Strong background Insufficient wash 1.

  • Page 20: Technical Support

    5. Technical Support Visit the GenScript web site at www.genscript.com for: 1. Technical resources, including manuals, vector maps and sequences, application notes, MSDSs, FAQs, formulations, citations, handbooks, etc. 2. Complete technical support contact information 3. Access to the GenScript Online Catalog 4.

  • Page 21: Appendix

    Appendix A-1. Buffer recipes eZwest cleaning solution Recipe (0.1M NaOH, 10% ethanol) NaOH 100 mL Ethanol Add water to Mix thoroughly Regular wash buffer Recipe 1× PBST / TBST 7.5 g EDTA·2Na Mix thoroughly Recipe Blocking buffer (50 mL) 2.5 g Dry milk (defat) 50 mL 1 X PBS...
  • Page 22 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188 1-877-436-7274 FAX: 1-732-5185-5150 Email: product@genscript.com...

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