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Related Manuals for GenScript eStain 2.0
Summary of Contents for GenScript eStain 2.0
- Page 1 ® For fast Coomassie blue staining of proteins in mini polyacrylamide gels Aug 15, 2014 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188, 732-885-9688 Fax: 732-210-0262, 732-885-5878 Email: product@genscript.com...
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Page 2: Table Of Contents
® eStain 2.0 Protein Staining System www.genscript.com Table of Contents Product Contents Product Specifications eStain ® 2.0 Protein Staining Device Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix... -
Page 3: Product Contents
Examine the unit carefully for any damage incurred during transit. File any the Instrument damage claims with the carrier. The warranty does not cover in-transit The following eStain ® 2.0 Graphite Electrode is available from GenScript: eStain ® 2.0 Graphite damage. -
Page 4: Product Specifications
Electrode Specifications electrode of eStain ® 2.0 Protein Staining Device and available separately Materials: Acrylonitrile butadiene styrene, Polycarbonate, from GenScript. The specifications for eStain ® 2.0 Graphite Electrode are Aluminum, Titanium, Plasticized silicone. listed below: Operating Temperature: Room temperature Dimensions: 100 mm (l) ×... -
Page 5: 2.0 Protein Staining Device
® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com eStain ® 2.0 Protein Staining Device eStain ® 2.0 Protein Staining Device, continued Front View of Control Panel of The front top view showing various parts of the eStain ®... -
Page 6: Introduction
Protein Staining Pads are used to perform electric staining of proteins in gels with Coomassie blue dye. Two types of eStain ® Protein Staining Pads, R-250 and G-250, are available separately from GenScript and for different requirements. Each pack of eStain ® Protein Staining Pad contains an eStain ®... -
Page 7: Quick Reference Guide
® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Introduction, Quick Reference Guide continued System Mechanism, Schematic mechanism of eStain ® 2.0 Protein Staining System showing the A quick reference guide for operating the eStain ®... -
Page 8: Protocols
® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Protocols Protocols, continued Recommendations Follow these recommendations for best results: Installing the Note: For a new eStain ® 2.0 Protein Staining Device, two pieces of Sponge Cushion and one unit of eStain ®... - Page 9 ® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Protocols, Protocols, continued continued 1. Open the closed lid by pressing the Open button. Assembling Staining 5. Place the gel on the eStain ® Anode Pad. Gently remove air bubbles...
- Page 10 ® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Protocols, Protocols, continued continued Performing Electric After assembling the staining stack, perform electric staining as described below. Performing Electric 3. At the end of the staining, current automatically shuts off and the eStain ®...
- Page 11 ® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Protocols, Protocols, continued continued Replacing the eStain ® During electric staining process, the eStain ® 2.0 Graphite Electrode will absorb ions Replacing the eStain ® 4. Take a spring from the bag, and install it on the electrode column which...
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Page 12: Troubleshooting
® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Protocols, Troubleshooting continued Replacing the eStain ® 6. Close the lid and switch On the eStain ® 2.0 Protein Staining Device. Press and hold Problem Cause... -
Page 13: Examples Of Results
2.0 Protein Staining System www.genscript.com 2.0 Protein Staining System www.genscript.com ® ® eStain eStain Troubleshooting, Examples of Results continued Tris-Glycine Gel Samples were separated on a 12% Tris-Glycine gel and stained using eStain ® Problem Cause Solution Ring-like spots observed on 1. -
Page 14: Technical Support
If any defects occur in the product during this warranty period, 2. Complete technical support contact information GenScript will, at its option, repair, replace, or refund the purchase price of 3. Access to the GenScript Online Catalog this product at no charge to you. The following defects, however, are 4. -
Page 15: Appendix
® ® eStain 2.0 Protein Staining System www.genscript.com eStain 2.0 Protein Staining System www.genscript.com Appendix Appendix, continued - 25 - - 26 -... - Page 16 ® eStain 2.0 Protein Staining System www.genscript.com Appendix, continued - 27 -...
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