GenScript eStain L1 Manual

Protein staining system
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GenScript USA Inc.
860 Centennial Ave. Piscataway, NJ 08854
Tel: 732-885-9188 732-885-9688
Fax: 732-210-0262 732-885-5878
Email: product@genscript.com
eStain® L1
Protein Staining System
For fast coomassie blue staining of protein in mini polyacrylamide gels

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Summary of Contents for GenScript eStain L1

  • Page 1 L1 Protein Staining System For fast coomassie blue staining of protein in mini polyacrylamide gels GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188 732-885-9688 Fax: 732-210-0262 732-885-5878 Email: product@genscript.com...
  • Page 2: Table Of Contents

    Table of Contents Important Notice Warranty 1. Overview 1.1 Instrument Overview 1.2 Instrument Specifications 1.3 Ordering Information 1.4 Maintenance 2. Instructions 2.1 Instrument display and feature locations 2.2 Pre-Programmed Staining 2.3 Advanced Instruction 2.3.1 Working Interface 2.3.2 Method Interface 2.3.3 Parameters Interface 2.3.4 Engineering Interface 3.
  • Page 3: Important Notice Warranty

    Please verify that all parts listed below are included within your package GenScript warrants that eStain L1 Protein Staining Device will be free from defects in material and workmanship for a period of one year from the date of purchase or eStain ®...
  • Page 4: Overview

    10 minutes or less. Built-in Features: Digital display, alarm, press key, light LED eStain L1 protein PAGE gel staining system consists of two parts, one is the Suitable for fast Coomassie blue staining of proteins in mini Compatibility:...
  • Page 5: Ordering Information

    L1 Liquid container solutions 10 L L00661-10 eStain L1 Small Two Pass Screw Cap (Fit 5 L and 10 L container) 3 pk L00663 *Cleaning Solution Per 1L eStain L1 Gel Holder...
  • Page 6: Instrument Display And Feature Locations

    2. Instruction contents 2.1 Instrument display and feature locations eStain ® L1 Protein Staining eStain ® L1 Protein Staining Device Keypad as shown in the following Device Keypad SETTING Key eStain L1 Protein Staining ® Device TOP View UP Key Channel A START Key DOWN Key Channel B START Key...
  • Page 7: Pre-Programmed Staining

    Preparation before eStain L1 Protein Staining Kit (Cat. No. L00659-1) or eStain L1 Destaining Solution (Cat. No. M00549-1) can be used directly. If you are buying an eStain L1 Staining concentrated Protein Staining Kit (Cat. No. L00669L), please dilute as instructed below before use.
  • Page 8 The axis of the gel holder Examples Uniformity Test Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (Genscript, M41210) 5. Pre-wet a piece of filter paper (provided) with Samples: distilled water and place it on top of the gel. Program: Pre-programmed Stain Time: 9 min 30 s 6.
  • Page 9: Advanced Instruction

    2.2 Pre-Programmed Staining 2.3 Advanced Instruction The instrument has four interfaces: Working interface, Method Interface, Sensitivity Test Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (Genscript, M41210) Parameters Interface, and Engineering Interface Program: Pre-programmed Stain Time: 9 min 30 s 2.3.1 Working...
  • Page 10: Method Interface

    2. Instruction contents 2.3 Advanced Instruction 2.3.2 Method eStain L1 has 7 methods: Stain, Destain, Equilibrium and 4 custom methods. Follow 2.3.3 Parameters Equilibrium cycle number Program Equilibrium duration the instruction below to enter the Method Interface. Interface Interface Staining cycles...
  • Page 11: Troubleshooting

    Please shorten the tube or value. make a slope cut at the end of the tube. Users are advised to contact GenScript to replace the 4. Automatic adjustment due to corresponding components, so the pressure changes. No as to avoid unnecessary losses special actions required.
  • Page 12: Technical Support

    MSDSs, FAQs, formulations, citations, handbooks, etc. 3. If the PAGE gels contain high 2. Complete technical support contact information concentration of ions or with 3. Access to the GenScript Online Catalog reused electrophoresis buffer, 4. Additional product information and special offers please run an equilibrating program before staining.

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