Related Manuals for GenScript eStain
Summary of Contents for GenScript eStain
- Page 1 The Biology CRO Protein Staining System For electric staining of proteins in mini polyacrylamide gels Version 2.0 June 4, 2010 User Manual...
-
Page 2: Table Of Contents
Protein Staining System www.genscript.com Table of Contents Product Contents Product Specifications eStain Protein Staining Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty... -
Page 3: Product Contents
Protein Staining Pads, you will receive the components listed in the table below. Store the eStain Protein Staining Pads at room temperature. For best results, use the eStain Staining Pads before the expiration date printed on the package. -1 -... - Page 4 Anode Pad each pack eStain Protein Staining Pad (G-250) contains: 1×eStain Cathode Pad (G-250) 1×eStain Anode Pad The following eStain Graphite Electrode is available from GenScript: eStain Graphite Electrode Product Cat. No. eStain Graphite Electrode (11mm, 1-pak) L02014 For best results, when a Graphite Electrode was used for 200 times of electric staining, replace it with a new one.
-
Page 5: Product Specifications
Avoid acetone, dimethyl sulfoxide, and acetic acid. These reagents can erode or damage the device. eStain Protein Staining The eStain Protein Staining Pads are used with the eStain Protein Staining Pads Specifications Device. The specifications of the eStain Protein Staining Pads are listed below:... - Page 6 Product Specifications, continued eStain Graphite The eStain Graphite Electrode is used as the replaceable anode of eStain Electrode Specifications Protein Staining Device and available separately from GenScript. The specifications for eStain Graphite Electrode are listed below: eStain Graphite Electrode (11mm) Dimensions: 100 mm (l) ×90 mm (w) ×11mm (h)
-
Page 7: Protein Staining Device
Protein Staining System www.genscript.com eStain Protein Staining Device Front View of The front top view showing various parts of the eStain Protein Staining Device eStain Device is shown below. Control Panel Handle Bar Rear View of The rear view showing various parts of the eStain... - Page 8 The Time button is used to set running time and toggle between staining mode and numbering mode. The Start/Stop button is used to activate/stop the staining program. Inside View of The inside view showing various parts of the eStain Protein Staining eStain Device...
-
Page 9: Accessory Products
Cat. No. eStain Graphite Electrode (11mm, 1-pak) 1 unit L02014 Additional Products The precast Express PAGE Gels as well as premade buffers or buffer powders are available from GenScript. For details, contact Technical Support or visit www.genscript.com. - 7 -... -
Page 10: Introduction
The eStain Protein Staining Pads are disposable pads to perform electric staining of proteins in gels with Coomassie blue dye. Two types of eStain Protein Staining Pad, R-250 and G-250, are available separately from GenScript and for different requirements. Each pack eStain... - Page 11 Anode Pad act as ion reservoirs that contain the appropriate anode and cathode buffers incorporated into the gel matrix. Simultaneously the eStain Cathode Pad presoaked with proprietary cathode buffer containing CBB dye R-250 or G250, also supplies negatively charged staining reagents. The high...
- Page 12 Graphite Anode - 4e + 2H Features Important features of the eStain Protein Staining System are listed below: · Unique electric staining technology created for fast, reliable and efficient protein gel staining in as few as 7 minutes. · User-friendly electric staining unit allowing for easy and convenient procedures.
-
Page 13: Quick Reference Guide
Protein Staining System www.genscript.com Quick Reference Guide A quick reference guide for operating the eStain Protein Staining Device Introduction is provided below. Mode Action Sound Light Display eStain plugged in Connect eStain – Steady upper Default Device to an electrical... -
Page 14: Protocols
Use only properly grounded AC outlets and power cords. 6. When the electrophoresis of your samples is almost complete, press the power switch (located on the rear of the device) to turn ON the eStain Protein Staining Device. The upper status light is on indicating you are using staining mode. - Page 15 Protein Staining Pad from the eStain Protein Staining Pads box and tear the laminated sealing of the package. Remove the two small packages respectively labeled as 1 x eStain Cathode Pad R-250 (or G-250), and 1 x eStain Anode Pad. - 13 -...
- Page 16 Protein Staining System www.genscript.com Protocols, continued Assembling Staining 3. Tear the sealing of the 1 x eStain Anode Pad package. Remove the Stack with eStain eStain Anode Pad from the package and place it on the anode plate of Pads and Pre-run Gel, the eStain Protein Staining Device.
- Page 17 Cathode Pad from the package and place it on the gel. 8. Pull back the handle bar to close the lid of eStain Device while keeping the bar vertical to the lid, and then return the bar to horizontal position.
- Page 18 Protein Staining System www.genscript.com Protocols, continued After assembling the staining stack, perform electric staining as described Performing electric staining below. 1. Press the Time button to set appropriate running time based on the gel thickness (see table below). If an undesired running time is set by mistake, press Reset button to clear the wrong time, and then press again the Time button to choose the desired running time.
- Page 19 Protocols, continued Performing electric 3. At the end of the staining, current automatically shuts off and the eStain staining, continued Protein Staining Device signals the end of staining with repeated beeping sounds. Upper status light stops flashing and the Digital Display shows text (000).
- Page 20 Protocols, continued Replacing the eStain During electric staining process, the eStain Graphite Electrode will absorb ions Graphite Electrode from anode pad and as well as lose carbon composition, thereby changing the characteristics of the Graphite Electrode and composition of the anode buffer.
- Page 21 Protein Staining System www.genscript.com Protocols, continued Replacing the eStain 4. Tear the sealing of a new eStain Graphite Electrode package and take the Graphite Electrode, new Graphite Electrode out of the package. Place the new Graphite Electrode continued into the anode tank and close the lid of the device.
- Page 22 6. Press Reset button to zero the staining times. Replacing the eStain Graphite Electrode, continued After successfully installing Graphite Electrode into the eStain Device, you are ready to use the eStain Device for staining applications again. Note: For the best results, after using Graphite Electrode per 40 times of electric...
-
Page 23: Troubleshooting
Protein Staining System www.genscript.com eStain Troubleshooting Review the information below to troubleshoot your experiments using the eStain Introduction Protein Staining Device and eStain Protein Staining Pad. Problem Cause Solution The upper status light doesn’t Incomplete electric circuit due Ensure the staining stack is... -
Page 24: Examples Of Results
Protein Staining System www.genscript.com eStain Examples of Results Tris-Glycine gel Samples were separated on a 12% Tris-Glycine gel and stained using eStain results Protein Staining System (R-250). Lane 1 500ng rabbit IgG Lane 2 10μl lysate E.coli Lane 3 200ng reduced BSA... -
Page 25: Technical Support
1. Technical resources, including manuals, vector maps and sequences, application notes, MSDSs, FAQs, formulations, citations, handbooks, etc. 2. Complete technical support contact information 3. Access to the GenScript Online Catalog 4. Additional product information and special offers Contact Us For more information or technical assistance, call, write, fax, or email. -
Page 26: Warranty
If any defects occur in the product during this warranty period, GenScript will, at its option, repair, replace, or refund the purchase price of this product at no charge to you. The following defects, however, are specifically excluded: 1. - Page 27 GenScript USA Inc. 120 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188, 732-885-9688 Fax: 732-210-0262, 732-885-5878 Email: estain@genscript.com...