GenScript eZwest Lite Manual
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GenScript eZwest Lite Manual

Automated western device
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Automated Western Device

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Summary of Contents for GenScript eZwest Lite

  • Page 1 Automated Western Device...

  • Page 3: Table Of Contents

    Version. 20200623 Table of Contents Components and initial setup ................3 Warranty ......................4 Instrument Overview .................. 5 Instrument overview ................5 Features and Benefits ..................5 Specifications..................6 Ordering information ................6 Safety & Maintenance ................7 Instructions ....................8 Instrument display and feature location ..........

  • Page 5: Components And Initial Setup

    Description Check and make sure the power plug matches the outlet Place eZwest Lite device on a steady and levelled lab bench Keep the vicinity of the instrument clean and ventilated Make sure the power switch is OFF before plug in the instrument...

  • Page 6: Warranty

    (10,000 minutes), whichever comes first. If any defects occur during this warranty period GenScript will at its option, repair, replace, or refund the purchase price of the product at no charge to you.

  • Page 7: Instrument Overview

    1. Instrument Overview 1.1 Instrument overview eZwest Lite is a fully automated blotting device that frees researchers from the manual labor of traditional western protocol. eZwest Lite can be programmed to run a sequence of western blot membrane incubation steps automatically including: blocking, primary antibody and secondary antibody incubations and washing.

  • Page 8: Specifications

    1.2 Specifications eZwest Lite automated western device weight： 6.48 Kg Dimensions(LxWxH)： 230×230×230 mm, 9.06x9.06x9.06 in Electrical Requirements： 100-120 V or 220-240 V，50/60 Hz Operating temperature： 15-40°C, 59-104°F Max membrane size(WxH) 90x83 mm, 3.5x3.2 in Throughput 1 channel for up to 2 membranes Application：...

  • Page 9: Safety & Maintenance

    1.4 Safety & Maintenance To ensure optimal performance, we recommend regularly cleaning the eZwest instrument. Component Description eZwest After each use, please clean the device following the device: procedures below. routine 1. Insert the membrane cassette into the device. cleaning 2.

  • Page 10: Instructions

    2. Instructions 2.1 Instrument display and feature location eZwest Lite automated western device Fig.1 Front view Fig.2 Back view...
  • Page 11 Fig.3 Top view Fig.4 Membrane cassette Fig.5 Display (main menu)

  • Page 12: Basic Operation

    Fig.6 Display (Method view) 2.2 Basic operation Save cassette ID when using the cassette for the first time 1. Position the membrane cassette as shown in Fig.7 2. Switch on the device, insert the membrane cassette into the device. Make sure the pins are pushed all the way in, and the side with the “eZwest”...

  • Page 13: View And Adjust Method

    3. After the cassette is inserted, you will be asked if you want to “save ID”. Press OK to save cassette ID as show in Fig.8 Note：Each eZwest Lite device could save up to 5 cassette IDs. Fig.8 View and adjust method 1.
  • Page 14 3. Select a step to show and modify the associated details. Note: Each Method may be programmed with 7 steps. Fig.11 4. Each step within a Method is customizable : a. Reagent option Fig.12 b. Number of Cycles required to repeat the step Fig.13...
  • Page 15 c. Run time for each cycle in minutes Fig.14 d. Pump Speed for recirculating the liquid flow through the membrane cassette Fig.15 5. Once you have finished setting up your Method, press “Save”...

  • Page 16: Set Cassette Specific Method

    Set cassette specific method The eZwest Lite device can store up to four dedicated methods for each cassette. Upon cassette installation, select the associated method. 1. Method settings can be changed as described above 2. Change name of membrane cassette a.
  • Page 17 2. Place the prepared solutions into eZwest Lite device as indicated below. Note: Assure all fittings and caps are tightly sealed. Fig.18 3. Open the membrane cassette: release the latch by rotating it up as shown in Fig.19(i), lift the latch and pull it towards the top of the cassette as in Fig.19(ii), the opened cassette is shown in Fig.19(iii).
  • Page 18 the high MW side aligning towards the bottom edge of the cassette as Fig.20(ii). Place a spacer across the high MW end (bottom) of the membrane along the membrane edge. b. Processing two membranes simultaneously: Place the first membrane as described in a. Then place a second spacer across the low MW side of the first membrane along the membrane edge.
  • Page 19 7. Check and ensure all the tubes and bottles contain adequate amount of reagents. Warning: insufficient buffer or incorrect solution placement may result in experiment failure. 8. Select the method on the main menu and click “START” to run 9. Once method finish, click “FINISH”, and replace the membrane cassette with cleaning cassette.

  • Page 20: Sample Protocols

    3. Sample Protocols Quick Protocol: Requires a sensitive antibody and/or higher abundant protein. All steps performed at room temperature. Step Cycles Time Pump Speed Blocking 30 min Normal Wash 3 min Normal 1°Antibody 60 min Normal Wash 3 min Normal 2°Antibody 30 min Normal...
  • Page 21 1° Antibody: Remove membrane and incubate with primary antibody overnight at 4°C with shaking The following day, insert membrane into eZwest and run the following method at room temperature: Step Cycles Time Pump Speed Wash 3 min Normal 2°Antibody 60 min Normal Wash 3 min...

  • Page 22: Troubleshooting And Faqs

    (set to normal) Weak signal 1. Antibody is too 1. Reduce antibody diluted dilution factor 2. Instrument needs 2. Clean eZwest Lite cleaning instrument 3. Insufficient 3. Increase antibody antibody incubation time or incubation time reduce incubation...

  • Page 23: Technical Support

    5. Technical Support Visit the GenScript web site at www.genscript.com for: 1. Technical resources, including manuals, vector maps and sequences, application notes, MSDSs, FAQs, formulations, citations, handbooks, etc. 2. Complete technical support contact information 3. Access to the GenScript Online Catalog 4.

  • Page 24: Appendix

    Appendix A-1. Buffer recipes eZwest cleaning solution (0.1M NaOH, 10% ethanol) Recipe NaOH Ethanol 100 ml Add water to Mix thoroughly Regular wash buffer Recipe 1× PBST / TBST 10x TBS buffer Recipe Tris base NaCl Dissolve in 900mL water, pH to 7.4 with 12N HCl Add water to 1x TBST buffer...
  • Page 25 1x PBST buffer Recipe 10× PBS 100mL Tween20 0.5mL Add water 900mL Mix thoroughly, pH should be around 7.4...
  • Page 28 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188 | 1-877-436-7274 FAX: 1-732-5185-5150 Email: product@genscript.com...

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