Related Manuals for GenScript eStain L1
Summary of Contents for GenScript eStain L1
- Page 1 eStain Protein Staining System For fast coomassie blue staining of protein in mini polyacrylamide gels Version 09302022...
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Page 3: Table Of Contents
Table of Contents Important Notice Warranty 1. Overview 1.1 Instrument Overview 1.2 Instrument Specifications 1.3 Ordering Information 1.4 Maintenance 2. Instructions 2.1 Instrument display and feature locations 2.2 General guidelines and Buffer preparation 2.3 Using the Pre-programmed Staining method 2.4 Advanced Instruction 2.4.1 Working Interface 2.4.2 Method Interface 2.4.3 Parameters Interface... -
Page 4: Important Notice
Important Notice Please verify that all parts listed below are included within your package L1 Protein eStain Cat. NO. Quantity Component Staining Device and L00657 eStain L1 Protein Staining Device accessories L00658 eStain Gel holder (Installed inside the device) Power Cord Forceps Shovel (for cutting gels) Stainless steel Tray... -
Page 5: Warranty
Notice：Damage caused by improper transportation, or any of the following actions are excluded: • Improper operation. • Repair or modification done by any other party than GenScript or an authorized agent • Use of fittings or other spare parts supplied by any other party than GenScript. -
Page 6: Overview
L1 is a highly efficient protein PAGE gel staining system, which uses Coomassie Brilliant Blue and a patented protein staining technology developedby Overview Genscript. eStain L1 staining system integrates the traditional three steps of fixing- staining-destaining into one step and can stain/destain two protein PAGE gels simultaneously in 10 minutes or less. -
Page 7: Instrument Specifications
1.2 Instrument eStain L1 Protein Staining Device Specifications Weight: 7.58 Kg Dimensions: 410 mm (L) × 270 mm (W) × 260 mm (H) Electrical Parameters: 110-120 V, 220-240 V, 50/60 Hz, 10 A Built-in Features: Digital display, alarm, press key, light LED Suitable for fast Coomassie blue staining of proteins in mini Compatibility: polyacrylamide gels... -
Page 8: Ordering Information
1. Overview 1.3 Ordering Information Consumables Package Cat. NO. Component 1 kit L00753 eStain L1C Staining Kit Some reagents may crystallize at low temperature, equilibrate and ensure the reagent is fully dissolved before use eStain L1C Staining Kit Size Quantity Cat. -
Page 9: Maintenance
1.4 Maintenance To ensure the quality of Component Maintenance description gel staining, we Gel Holder 1. Per 50 times use, immerse the gel holder in 75% alcohol (or recommend regular per 900 ml 75% ethanol and 100 ml concentrated cleaning maintenance of the solution) for 4 hours. -
Page 10: Instructions
2. Instruction contents 2.1 Instrument display and feature locations eStain L1 Protein Staining Device TOP View Display Keypad Staining Solution inlet Dastaining Solution inlet Waste Outlet Channel A and Gel Holder Channel B and Gel Holder eStain L1 Protein Staining Device BACK View Power Switch Power Input... - Page 11 eStain L1 Protein eStain L1 Protein Staining Device Keypad as shown in the following Staining Device Keypad SETTING Key UP Key Channel A START Key DOWN Key Channel B START Key Keypad function Interface Function Operation key Working Interface Method Interface Increase cycle number / Increase reaction Parameters Interface time...
- Page 12 2. Instruction contents 2.1 Instrument display and feature locations Keypad function Interface Function Operation key Working Interface Press all together until beeps, Channel A starts to run Destain Program Press all together until beeps, Channel B Working Interface starts to run Destain Program Press all together until beeps, Channel A Working Interface starts to run Equilibrium Program...
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Page 13: General Guidelines And Buffer Preparation
2.2 General guidelines and Buffer Preparation Use the following recommendations for best results: • Wear gloves at all times during the entire staining procedures to prevent contamination of filter papers and gels. • Always use the kits before the specified expiration date printed on the package. -
Page 14: Using The Pre-Programmed Staining Method
2. Instruction contents 2.3 Using the Pre-programmed Staining method Staining procedure 1. Add distilled water into the tray provided 2. After electrophoresis, carefully remove the pre-run gel from the gel cassette and briefly rinse the gel with distilled water for 1 min. Notice: If you’re using precast gels from Life Technology, please remove the upper thicker part of the gel beneath the comb before staining to ensure the gel is in close contact with the filter paper. - Page 15 10. The channel stops flashing and the screen returns to normal. Examples Uniformity Test Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (Genscript, M41210) Samples: PAGE-MASTER Protein Standard Plus, 5 μl (GenScript, MM1397-500) Program: Pre-programmed Stain Time: 9 min 30 s...
- Page 16 2. Instruction contents 2.3 Using the Pre-programmed Staining method Sensitivity Test Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (Genscript, M41210) Program: Pre-programmed Stain Time: 9 min 30 s 1. E.coli cell lysis 2. PAGE-MASTER Protein Standard Plus, 5 μl (GenScript, MM1397-500) 3.
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Page 17: Advanced Instruction
2.4 Advanced Instruction The instrument has four interfaces: Working interface, Method Interface, Parameters Interface, and Engineering Interface 2.4.1 Working After turning on the power, the instrument enters the Working Interface as illustrated below: Interface Present state The remainder of channel of current program Information of The program of... -
Page 18: Working Interface
2. Instruction contents 2.4 Advanced Instruction 2.4.2 Method eStain L1 has 7 methods: Stain, Destain, Equilibrium and 4 custom Interface methods. Followthe instruction below to enter the Method Interface. The List of Programs Current Program Choosed Channel 1. Press UP or DOWN to select the channel to highlight 2. -
Page 19: Parameters Interface
2.4.3 Parameters Equilibrium cycle number Program Equilibrium duration Interface Staining cycles Staining duration Destaining cycles Destaining duration The workflow of eStain L1 contains equilibrium cycles, staining cycles and destaining cycles. In this interface, you can set the number of cycles for the equilibrium, staining and destaining program, and the duration for each cycle Note: Cycle number can be set to 0-4 range and each cycle time can be set to 0-5 minutes. -
Page 20: Troubleshooting
The lifetime of the part highlighted in blue has reached its recommended maximum value. Users are advised to contact GenScript to replace the corresponding components, so as to avoid unnecessary losses Return to working interface by press any button. During Not enough equilibrium/destaining solution in channel A. - Page 21 The warning means there is leakage in the machine. For your safety, please power off the machine and contact GenScript immediately. Do not try to move or use the machine before the problem is solved. - 18 -...
- Page 22 3. Troubleshooting Description of the problem Solution Problems Other devicer Q7 machine repeatedly gives Please check the tubes to see if "Not enough solutions" warnings. elated issues. there are twists and that the end of the tubes form a seal with the (Q7~Q8) bottom of the container, If not, please enter the "engineering...
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Page 23: Technical Support
1. Technical resources, including manuals, vector maps and sequences, application notes, MSDSs, FAQs, formulations, citations, handbooks, etc. 2. Complete technical support contact information 3. Access to the GenScript Online Catalog 4. Additional product information and special offers For more information or technical assistance, call, write, fax, or email. - Page 24 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188 732-885-9688 Fax: 732-210-0262 732-885-5878 Email: product@genscript.com...
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