GenScript eBlot L2 User Manual
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GenScript eBlot L2 User Manual

Fast protein transfer device for high quality wet protein transfer
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User Manual
eBlot
TM
Fast Protein Transfer Device
For High Quality Wet Protein Transfer
Version：V1
Cat. No：L00981
L2

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Summary of Contents for GenScript eBlot L2

  • Page 1 User Manual eBlot Fast Protein Transfer Device For High Quality Wet Protein Transfer Version：V1 Cat. No：L00981...

  • Page 3: Table Of Contents

    CONTENTS Important Notice Quick Guide 1. Product Overview 1.1 Instrument Overview and Features 1.2 Components, Specifications and Installation 2. Device and Interface Display 2.1 Device and Transfer Cassette 2.2 Interface Display and Program Setting 3. Device Operation and Application Data 3.1 Reagent Preparation 3.2 Program Selection Guide 3.3 Operation Instructions...

  • Page 4: Important Notice

    Version：V1; Date: 20240115 Please be aware that GenScript will not be held responsible for any personal injury or damage to the instrument caused by failure to follow the instructions provided in the manual.

  • Page 5: Quick Guide

    Other Materials Diluted eBlot L2 Transfer Buffer (300 Pre-run PAGE gel mL/Standard transfer） NC membrane or Pre-activated PVDF Diluted eBlot L2 Equilibrium Buffer (60 mL membrane per membrane in the plastic container Dry transfer sponges (2 pieces per provided) transfer) Distilled water（250 mL per transfer in the...
  • Page 6 Rinse the gel cassette under running water (5 seconds) to remove remaining running buffer. Open the gel cassette and cut off the gel well fingers. Place the gel and membrane in the eBlot L2 Equilibrium buffer for 1 minute*. Note: a.
  • Page 7 Start Transfer Close the transfer cassette, and insert the assembled cassette into the selected channel with the scale lines completely covered. Press to initiate the transfer program. Note: a. The latch and scale lines are facing toward the user. b. Start transfer no longer than 5 minutes after the assembly of transfer sandwich. c.

  • Page 8: Product Overview

    1. Product Overview 1.1 Instrument overview eBlot™ L2 Fast Transfer System is the second-generation protein transfer instrument engineered to efficiently handle the transfer of proteins. eBlot™ L2 can simultaneously complete the transfer process for up to 4 mini gels or 2 midi gels in 5-15 minutes. The unique eBlot technology seamlessly combines the benefits of traditional wet transfer and semidry/dry transfer methods, ensuring with very short transfer times producing exceptional transfer efficiency and consistent results for proteins of all sizes.

  • Page 9: Components, Specifications And Installation

    L2 Fast Wet Transfer System includes the following components. Please carefully check the components before installation. Products Catalog Number Quantity eBlot L2 Fast Transfer Device L00980 eBlot L2 Transfer Cassette, midi L00982 Power cord Forceps Spatula Silver tray Roller Silicon tubing...
  • Page 10 Silver tray： Stainless steel Note: Avoid contact with acid, alkaline, acetone or any other reagents that might erode or damage the exterior surface of the device. eBlot L2 Transfer Cassette, midi Catalog Number： L00982 Dimension： 205 mm (L)× 160 mm (W)× 40 mm (H) Weight：...
  • Page 11 Installation Users must follow the instructions below when installing the instrument Steps Descriptions Check if the power cord matches with the outlet Place eBlot L2 on a levelled laboratory bench Maintain cleanliness and ventilation around the instrument, especially behind the device Ensure the power switch is off Connect the inlet and outlet tubes to the color-matched reagent containers as followed:...

  • Page 12: Device And Interface Display

    Waste outlet 2 Waste outlet 1 Figure 1. eBlot L2 Device Figure 2. eBlot L2 Back View Latch Transfer cathode Scale line Red sealing strip Transfer anode Transfer cassette (Closed) Transfer cassette (Open) Figure 3. eBlot L2 Transfer Cassette midi...

  • Page 13: Interface Display And Program Setting

    2.2 Interface Display and Program Setting 2.2.1 Interface Display eBlot™ L2 has three interfaces: main interface, program setting and system setting. The initial interface after powered on is the main interface which shows Channel A and B, and the selected programs. Main Interface Figure 4.
  • Page 14 Program Selection Interface Press to enter the program selection interface. SELECT Fig 5. Program selection interface for channel B Interface notation Description List of program options, with the selected one highlighted Program settings Channel B Current channel SELECT Apply the selected program to the indicated channel and return to main interface EDIT Edit the selected program.
  • Page 15 Program Setting Interface Navigate into for program setting interface and select the program. to edit the program settings. EDIT Fig 6. Program Selection Fig 7. Program Editting Interface notation Description Buffer Cycles Cycle Choose number of transfer cycles Cycle 1 Type in transfer time.
  • Page 16 System Setting Interface Navigate into for system settings. Fig 8. System Setting Notation Description Language English Select between English/Chinese interface Drain Residue Reagents Drain Drain the residue reagents from the instruments tubing Reset Pump Setting Reset Reset to the default buffer inflow time Reload Default Method Reset to the default program settings of Standard, Short, Long protocols Default...
  • Page 17 2.2.2 Transfer Program Overview eBlot L2 contains 3 default programs (Standard, Short, Long), and 3 customizable programs for various transfer needs. eBlot L2 Default Program Protocols Standard Figure 9. Default Standard Program Short Figure 10. Default Short Program Long Figure 11. Default Long Program...

  • Page 18: Device Operation And Application Data

    3.1 Reagent Preparation Before you use the proprietary transfer reagent kit (Cat. No. L01015) on eBlot L2, dilute the concentrated transfer buffer (Cat. No. B0056) and the concentrated equilibrium buffer (Cat. No. B0057) according to the protocol below.

  • Page 19: Program Selection Guide

    0.22 μm PVDF/NC membranes are recommended for proteins ＜20 kDa. b. If you are using GenScript SurePAGE Mini or Midi Gel 4-12% or 4-20%，please select Standard program. c. Users can customize the transfer programs to optimize the transfer efficiency.

  • Page 20: Operation Instructions

    3.3 Operation Instructions 1. Prepare 60 mL 1X equilibrium buffer in the provided plastic container. 2. Prepare 250 mL distilled water in the provided tray. 3. Ensure adequate transfer buffer and regularly empty the waste container. Note：Each Standard program consumes approximately 300 mL 1X transfer buffer. 4.
  • Page 21 7. Place one dry sponge on the red anode side. Note： a. The anode side is marked with red sealing strip. b. Use dry sponges for transfer. Sponges should NOT be wet. c. Sponges are NOT reusable and should be discarded after the transfer.
  • Page 22 9. Gently remove bubbles between the gel and membrane using the roller dipped in the equilibrium buffer. Note：Do not press the gel to cause gel deformation. 10. Place another dry sponge on top of gel. Note：Do not frequently remove the top dry sponge to create bubbles between gel and membrane. Dry Sponge PVDF/NC Membrane Dry Sponge...
  • Page 23 12. Fully insert the assembled transfer cassette into the channel until the scale lines of the cassette completely covered as shown in Figure 18. Note： a. Note the proper orientation and loading of the cassette. The scale lines of the cassette should be facing towards the user.
  • Page 24 14. The device will beep when the countdown reaches 00:00 as shown in Figure 20. Press the stop button to return to the initial interface. FIgure 20. Transfer completed Remove the transfer cassette from the channel. Note：Slightly heated transfer cassette is normal. Open the cassette according to Step 4.

  • Page 25: Application Data

    3.4 Application Data Side-by-side comparison data between eBlot™ L2 Standard Program and traditional wet transfer using GenScript 4-12% Precast Gel. Traditional wet transfer：100V，60 min eBlot™ L2：2x5 min (NC membrane, 0.22 μm) (NC membrane , 0.22μm) EGFR ~150 kDa β-actin ~42 kDa...

  • Page 26: Device Maintenance

    4. Device Maintenance Regular maintenance is highly recommended to maintain the upkeep of the device and performance of protein transfer. Components Maintenance guide Transfer cassette After each transfer, rinse the cassette with water for 60 seconds and set dry on the bench. Perform system cleaning once every 100 transfers, or once a month for higher usage frequency.

  • Page 27: Troubleshooting

    2. Do not reuse PVDF membrane activation contamination membrane activation reagent reagent. (methanol, ethanol, isopropanol). 3. Do not reuse eBlot L2 equilibrium buffer. 3. Contamination in the eBlot L2 4. Do not reuse transfer sponges. equilibrium buffer. 5. Rinse the transfer cassette for 60 seconds 4.
  • Page 28 2. Increase transfer time or cycles bands appropriate 3. Choose lower concentration gels 0.22μm PVDF, eBlot L2: 2x5min 0.45μm PVDF, eBlot L2: 2x5min EGFR 150 kDa 1. Inappropriate membrane pore size 1. Use 0.22 μm PVDF/NC membrane if signal is Low signal for 2.
  • Page 29 3. Inspect if there are abnormal substances 3. Abnormal substances exist inside inside the transfer cassette; contact the transfer cassette that causes GenScript Tech Support if not resolved short circuits between anode and 4. Properly assemble the transfer sandwich cathode 5.
  • Page 30 GenScript transfer buffer spraying out, Technical Support triggering a liquid leakage warning liquid detection error pump error Hardware malfunction Please contact GenScript Tech Support valve error Integrated driver error...

  • Page 31: Consumables Offering

    L2 concentrated transfer reagents for 30 midi or 60 mini transfers with L01015-30 eBlot L2 Transfer Basic Kit, Midi 1 Kit standard method L01015-30, eBlot L2 Transfer Basic Kit, Midi Component name Size Quantity eBlot L2 Concentrated Transfer Buffer，5X 1.8 L eBlot L2 Equilibration Buffer (5X) 0.4 L...

  • Page 32: Related Products

    7. Related Products SurePAGE Mini&Midi Bis-Tris Precast Gel High Resolution and Fast Run Time eBlot Fast Wet eStain Fast Transfer System Staining System All size protein transfer Gel or membrane staining in 5-15 minutes in 10 minutes eZwest Automated Western Device Automated antibody incubation and washing Saving more time with...
  • Page 33 SurePAGE Bis-Tris Gel, Midi, 10pk/box Cat. No. Concentration Well format M00995 4-12% M00996 4-12% SurePAGE Bis-Tris Gel, Mini, 10pk/box Cat. No. Concentration Well format M00652 4-12% M00653 4-12% M00654 4-12% M00655 4-20% M00656 4-20% M00657 4-20% M00658 8-16% M00659 8-16% M00660 8-16% M00661...
  • Page 34 Other Electrophoresis Products Cat. No. Product Name Quantity L01001 GenBox Midi Electrophoresis Tank L01021 GenBox Mini Plus Electrophoresis Tank L01022 GenBox Midi/Mini Electrophoresis Tank L00780 GenBox Mini Electrophoresis tank L00781 GenBox Mini Blot module L00782 GenBox Mini Blot System M00138 Tris-MOPS-SDS Running Buffer Powder 5 PK/Box M00677...

  • Page 35: Warranty

    GenScript warrants that eBlot L2 to be free from defects in materials and workmanship under normal use and service for a period of twelve (12) months from the date of installation by GenScript or its authorized distributor. GenScript agrees within the warranty period, at its discretion, to repair defects in the material or workmanship or to furnish a repaired or refurbished product of equal value in exchange without a fee.
  • Page 36 Tel: +1-732-885-9188 Toll-Free: +1-877-436-7274 Fax: +1-732-210-0262 Email: support.US@genscript.com Address: 860 Centennial Ave. Piscataway NJ 08854 China Tel: +86-025-58897288 ext 5810 Fax: +86-025-58897288-5815 Email: product@genscript.com.cn Address: 28 YongXi Road, JiangNing District, Nanjing, China Asia/Pacific Tel: +65 3159 1898 Email: sales.AP@genscript.com Fax: +65 6491 5073...

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