GenScript eBlot L2 Quick Manual

eBlot™ L2 Fast Protein Transfer Device
Quick Guide
Step 1.
Reagents and Materials Preparation
・
Pre-run PAGE gel
・
eBlot L2 Transfer Buffer, 1X (300 mL per standard transfer)
・
eBlot L2 Equilibrium Buffer, 1X (60 mL per membrane in the
plastic container provided)
・
Distilled water (250 mL per transfer in the silver tray provided)
・
PVDF or Nitrocellulose membrane
・
Dry transfer sponges (2 pieces per transfer)
Note:
・
Regularly empty the waste container.
Step 3.
Wet PVDF/NC membrane with eBlot L2 Equilibrium
buffer for 1 minute (for PVDF membrane: pre-wet the
membrane with methanol, ethanol, or isopropanol
before equilibrium).
After gel electrophoresis, rinse the
gel cassette with water (5 seconds)
to remove any remaining running
buffer. Open the gel cassette and
cut off the gel well fingers. Place the
gel in the eBlot L2 Equilibrium
buffer for 1 minute*.
Note:
・
Cut off any uneven part of the gel
The gel equilibrium time should NOT exceed 5 minutes
*
Step 2.
Place the transfer cassette on the bench.
a
・
Release the cassette latch by rotating it upward.
・
Lift the latch and fully open the cassette cover to approximately >90°.
Note:
・
The anode side of the transfer cassette is marked with a red sealing strip.
Step 4.
On the red anode side, assemble the transfer sandwich in the following order: dry sponge,
equilibrated membrane, equilibrated gel. Use the roller to roll out bubbles between the
membrane and the gel, then place another piece of dry sponge on top of the gel.
Dry sponge
Gel
PVDF/NC membrane
Dry sponge
Note:
・
The sponge doesn't need to be pre-wet
・
Note the correct size of the components: Sponge ≥ Membrane ≥ Gel
・
Place the gel and membrane close to the bottom, with large molecular proteins close to the inlet/outlet end.
・
Avoid overlapping between two mini gels or two membranes.
GenScript USA Inc.
Email: product@genscript.com
Address: 860 Centennial Ave, Piscataway NJ, 08854 USA
Tel: 1-732-885-9188 | 1-877-436-7274 (Toll-Free)
Fax: 1-732-518-5150
b
Transfer 2 mini gels in one cassette Transfer 1 midi gel in one cassette
Inlet/outlet end
Bottom
Bottom
-
+
c
Inlet/outlet end