Advertisement
ADJUSTING THE COLLECTION SCHEDULE
Once the appropriate column has been selected and mounted to the AFC tower, the collection
schedule must be set.
1.
From the home screen press
volume, and buffer volume for collection. Press
save your parameters. Press
SETTING UP THE CAROUSEL
1.
Load the collection tubes onto the carousel with the lids facing inwards. Ensure you have
loaded enough tubes for your collection protocol.
2.
Gently place the carousel onto the carousel plate. Ensure that the carousel alignment
hole engages with the raised carousel alignment pin on the carousel plate.
Remember to label your tubes before placing them into the AFC carousel.
FLUSHING THE COLUMN
1.
The next screen will ask you to mount the column reservoir. Note: If using a qEVsingle, a
column reservoir is not needed.
2.
Press
OK
to continue, The next screen will ask you to load 1 mL of buffer onto the column.
If this is your first use of the column for the day, we recommend flushing of all columns
with fresh, filtered PBS buffer. Instead of loading 1 mL of buffer, load two column
volumes of buffer and press
of the buffer to pass through the column while on the 'Priming Column' screen, then press
OK
to continue.
SAMPLE LOADING AND INITIATING COLLECTION
1.
The AFC will ask you to have sufficient buffer available to run the column and to load the
sample.
2.
Ensure that there is no residual buffer left on the top of the column, remove any excess
with a pipette. Now add your sample.
It is important to only load the sample at this point. Adding buffer at the same time or
before the sample has fully entered the loading frit of the column will result in incorrect
sample loading.
3.
Press
OK
to continue. The column will start running and the carousel will move to the
buffer collection position. The lighting will change to
has started.
www.izon.com
COLLECTION
SCHEDULE, you can adjust the count,
-/+
START COLLECTION
OK
to continue. If you choose to flush the column, wait for all
and follow the on-screen prompts to
to begin.
red
to indicate that the collection
3
Advertisement
